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神经再生
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  nerve regeneration
Tissue engineering and peripheral nerve regeneration (III)
      
The process of the nerve regeneration was investigated by histological assessment, electrophysiological examination, and determination of wet weight recovery rate of the gastrocnemius muscle.
      
At the conclusion of 12 weeks, proximal and distal end of nerves were anastomosed by nerve regeneration and the conduit vanished completely.
      
The results suggest that PDLLA conduits may serve for peripheral nerve regeneration and PDLLA is a sort of hopeful candidate for tissue engineering.
      
The first axons to grow very early in the course of nerve regeneration belong primarily to the phrenic nerve which is thus reinnervating some of the denervated shoulder muscles.
      
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  regeneration of nerve
The regeneration of nerve fibres in pigs was studied three months after transsection and end-to-end suture of the dorsal and ventral spinal nerve roots L 5 and S 1.
      
The regeneration of nerve fibres in pigs was studied three months after transsection and end-to-end suture of the dorsal and ventral spinal nerve roots L 5 and S 1.
      
On the 7th day, mitosis of Schwann cells was observed and on the 250 th day, myelinated fibres were noticeably decreased in numbers, with regeneration of nerve fibres and an increase of collagen in the intercellular spaces.
      
Pathological alterations indicating demyelination, remyelination and regeneration of nerve fibers were occasionally observed.
      
Reappearance of epidermal fibers was delayed compared to the regeneration of nerve fibers in the sciatic nerve.
      
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IM: To study the promoting activity of 2 5 s murine nerve growth factor (NGF) for the regeneration of injuried sciatic nerve. METHODS: The sciatic nerves of mice and rats were crushed. The axon counts and differential axon counts were made. Evoked potential latency (NMEPL) was recorded at sites of proximal (Pro) and distal (Dis) segment of soleus muscles. RESULTS: In mice, NGF im 0 5-1 kBU·kg -1 increased the axon counts of regenerated nerves 20 d after crush; 2-4 kBU·kg -1 simultaneously alleviated...

IM: To study the promoting activity of 2 5 s murine nerve growth factor (NGF) for the regeneration of injuried sciatic nerve. METHODS: The sciatic nerves of mice and rats were crushed. The axon counts and differential axon counts were made. Evoked potential latency (NMEPL) was recorded at sites of proximal (Pro) and distal (Dis) segment of soleus muscles. RESULTS: In mice, NGF im 0 5-1 kBU·kg -1 increased the axon counts of regenerated nerves 20 d after crush; 2-4 kBU·kg -1 simultaneously alleviated the denervated atrophy of soleus muscles. In rats, NGF im increased the axon counts at dose of 1 kBU·kg -1 for 40 d, 2 kBU·kg -1 for 30 d and 40 d, and 4 kBU·kg -1 for 20 d, 30 d and 40 d after crush; the number of large axons of regenerated nerves were also increased by NGF im 2 kBU·kg -1 for 40 d and 4 kBU·kg -1 for 30 d and 40 d; Pro NMEPL was shortened by NGF 1, 2, and 4 kBU·kg -1 , Dis NMEPL was shortened by NGF 2 and 4 kBU·kg -1 for 40 d. CONCLUSION: NGF im evidently promotes the regeneration of injuried sciatic nerve, and attenuates the skeletal muscular atrophy caused by nerve injury.

目的:证实NGF促进坐骨神经再生的作用.方法:夹断小鼠和大鼠坐骨神经轴索,测再生轴索计数及分类,在比目鱼肌远(Dis)、近(Pro)端测神经肌肉电潜伏期(NMEPL).结果:小鼠NGFim05-1kBU·kg-120d增加轴索再生率,2-4kBU·kg-1减轻比目鱼肌萎缩.大鼠NGFim1(40d),2(30和40d),4(20,30,40d)kBU·kg-1均显著增加损伤神经的轴索再生率;高、中剂量增加粗轴索计数;各剂量均缩短DisNMEPL(20d,30d)和ProNMEPL(40d);高剂量在各时点使两者均缩短.结论:NGFim明显促进大鼠和小鼠坐骨神经损伤后再生并减轻骨骼肌萎缩.

bjective: X-ray radiation was done partially on the spinal cord of newborn Wistar rats at the third day after birth to study the effect of X-ray radiation on spinal neurons and oligodendrocytes.Method :The animals were divided into experimental and control groups, with X-ray dosage of 25,35,45 and 55 Gy once each on the experimental group.The general condition and motion function after radiation were observed.13 days after birth,the specimens of spinal cords were obtained for histological examination,quantitative...

bjective: X-ray radiation was done partially on the spinal cord of newborn Wistar rats at the third day after birth to study the effect of X-ray radiation on spinal neurons and oligodendrocytes.Method :The animals were divided into experimental and control groups, with X-ray dosage of 25,35,45 and 55 Gy once each on the experimental group.The general condition and motion function after radiation were observed.13 days after birth,the specimens of spinal cords were obtained for histological examination,quantitative analysis with image analyzer,as well as qualitativly observed with transmission electron microscope. Results: There were significant differences on numbers of oligodendrocytes between experimental and control group,as well as among experimental groups.(1)There were significant decreases of oligodendrocytes in radiated areas compared with normal areas in the vicinity, most prominent in the white matter;(2)There was extremely significant positive relativity between dosage of radiation and the amount of decrease of oligodendrocytes;(3)The dosage of X-ray over 35 Gy might lead to the impairment of spinal neurons;(4)The dosage of 25 Gy and 35 Gy could lessen the number of oligodendrocytes without injury to the spinal neurons. Conclusion: The inhibition effect of X-ray to oligodendrocytes provided an experimental basis for the research on mechanisms of neuron and spinal cord regeneration following injury.  

目的:通过X线照射新生(生后3天)Wistar大鼠部分脊髓,研究X线照射对脊髓神经元及少突胶质细胞的效应。方法:实验动物分为实验组、对照组。实验组分别给予一次25、35、45和55Gy的X线照射剂量,观察照射后动物一般状态及运动功能情况。取生后13天大鼠的已照射脊髓标本进行组织学评价,用图像分析仪对其定量;用透射电镜观察超微结构的变化。结果:对照组与实验组少突胶质细胞数量间差异,以及各实验组间的差异均具有非常显著性意义。(1)在X线辐射区与相邻正常区相比有明显的少突胶质细胞减少,以脊髓白质区减少最为明显;(2)X线照射剂量与少突胶质细胞数目减少程度有极显著正相关;(3)X线照射剂量超过35Gy可以造成脊髓神经元的损伤;(4)25、35Gy照射既可使少突胶质细胞减少,又不损伤神经元。结论:X线照射,可抑制少突胶质细胞生长。这为促进神经再生机理的研究,以及脊髓损伤后的再生提供了一定的实验基础。

Objective To obtain the effects of the L-NAME therapy in peripheral nerve regeneration .Methods Twenty four SD male rats were used in this study.Their bilateral sciatic nerves were cut off at 1cm from the site under the musculus piriformis then distant end of the nerve were cut off of 4mm.The two ends of nerve were bridged by silicone chamber.They were divided into two groups (silicone chamber injection abdomenal cavity injection )at random .Then each of them was divided into the right inject L-NAME one...

Objective To obtain the effects of the L-NAME therapy in peripheral nerve regeneration .Methods Twenty four SD male rats were used in this study.Their bilateral sciatic nerves were cut off at 1cm from the site under the musculus piriformis then distant end of the nerve were cut off of 4mm.The two ends of nerve were bridged by silicone chamber.They were divided into two groups (silicone chamber injection abdomenal cavity injection )at random .Then each of them was divided into the right inject L-NAME one and left inject NSL one in the group of the silicome chamber injection.Histologic examination was performed.Result L NAME can improve peripheral nerve regeneration .Conclusion L NAME can improve peripheral nerve regeneration.

目的 探索一氧化氮合成酶 (NOS)抑制剂对断裂周围神经再生的影响。方法 用 SD雄性大鼠 24只。在距梨状肌下缘 1cm处切断坐骨神经,远断端去除 4mm长,取硅胶管桥接两神经断端,并使其相距 4mm,随机分成硅胶管和腹腔注射两组。一组其右侧注入 10mg/kg亚硝基左旋精氨酸甲酯 (L-NAME),左侧注入等量生理盐水作对照。另组用同等剂量 L-NAME改为腹腔注射 10d。每组分成二半,分别于术后 1、 2月取材,作组织学检测。结果 发现 L-NAME对周围神经的再生有明显的促进作用,而局部注射药较腹腔注射给药神经再生优。结论 L-NAME能促进周围神经再生

 
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