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     A Preliminary Assay of Several Toxic Peptides from Amanita fuliginea by HPLC
     黑鹅膏菌(Amanita fuliginea)毒素的HPLC初步分离鉴定
短句来源
     The amanitatoxins from Amanita fuliginea were analyzed by a reversed- high performance liquid chromatography with a column C1 8and the mobile phase of a mixture of different concentration of0 .0 2 mol/L ammonium acetate acid- acetonitrile.
     本文报道了用反相高效液相色谱法 (HPLC)分离黑鹅膏实体内几种鹅膏毒素的结果 .
短句来源
     Six toxic peptides were purified and identified from the fruitingbody of A. fuliginea which includeα- amanitin, β- amanitin,phalloidin,phallacidin,phallisin,phalloin. The content were 5 .1 9mmol;
     采用 C1 8液相柱 ,以不同浓度的 0 .0 2 mol/L 乙酸铵—乙腈混合液为流动相 ,从黑鹅膏子实体中分离并鉴定出了 6种毒素 ,每公斤干重含量分别为 (mmol) :α-毒伞肽 :5 .1 9mmol;
短句来源
     The results of the heterozygosity were consistent with that of PIC. UPGMA tree was completed through analysis of D_A genetic distances.
     对DA遗传距离的计算表明,用UPGMA法进行聚类分析,结果4个鹅品种被聚为3类:道州黑鹅与酃县白鹅聚为第1类;
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  相似匹配句对
     Black Tulips
     郁金香
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     The Black Cat
    
短句来源
     A Preliminary Assay of Several Toxic Peptides from Amanita fuliginea by HPLC
     膏菌(Amanita fuliginea)毒素的HPLC初步分离鉴定
短句来源
     Nutrition of domestic geese
     家的营养
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     Studies on goose plague
     瘟的研究
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The amanitatoxins from Amanita fuliginea were analyzed by a reversed- high performance liquid chromatography with a column C1 8and the mobile phase of a mixture of different concentration of0 .0 2 mol/L ammonium acetate acid- acetonitrile.Six toxic peptides were purified and identified from the fruitingbody of A.fuliginea which includeα- amanitin, β- amanitin,phalloidin,phallacidin,phallisin,phalloin.The content were 5 .1 9mmol;1 . 1 7mmol;1 .5 0 mmol;0 .40 mmol;0 .2 6 mmol;0 .0 6 mmol respectively per...

The amanitatoxins from Amanita fuliginea were analyzed by a reversed- high performance liquid chromatography with a column C1 8and the mobile phase of a mixture of different concentration of0 .0 2 mol/L ammonium acetate acid- acetonitrile.Six toxic peptides were purified and identified from the fruitingbody of A.fuliginea which includeα- amanitin, β- amanitin,phalloidin,phallacidin,phallisin,phalloin.The content were 5 .1 9mmol;1 . 1 7mmol;1 .5 0 mmol;0 .40 mmol;0 .2 6 mmol;0 .0 6 mmol respectively per kilogram dried.

本文报道了用反相高效液相色谱法 (HPLC)分离黑鹅膏实体内几种鹅膏毒素的结果 .采用 C1 8液相柱 ,以不同浓度的 0 .0 2 mol/L 乙酸铵—乙腈混合液为流动相 ,从黑鹅膏子实体中分离并鉴定出了 6种毒素 ,每公斤干重含量分别为 (mmol) :α-毒伞肽 :5 .1 9mmol;β-毒伞肽 :1 .1 7mmol;phalloidin:1 .5 mmol;phallacidin:0 .40 mmol;phallisin:0 .2 6 mmol;phalloin:0 .0 6 mmol

Thirty-one microsatellite markers with medium polymorphisms were selected to detect the genetic diversity of 4 indigenous goose breeds in Hunan Province.According to the allele frequencies of 31 microsatellite sites,mean heterozygosity(H),polymorphism information content(PIC) and D_A genetic distances were calculated for each breed.The results showed that 25 of 31 microsatellite sites were middle polymorphic,so the 25 microsatellite markers were effective markers for analysis of genetic relationship among goose...

Thirty-one microsatellite markers with medium polymorphisms were selected to detect the genetic diversity of 4 indigenous goose breeds in Hunan Province.According to the allele frequencies of 31 microsatellite sites,mean heterozygosity(H),polymorphism information content(PIC) and D_A genetic distances were calculated for each breed.The results showed that 25 of 31 microsatellite sites were middle polymorphic,so the 25 microsatellite markers were effective markers for analysis of genetic relationship among goose breeds.The mean heterozygosity of 4 goose breeds all exceeded 0.5.The highest one was in the Lingxian White(0.6705),and the lowest one was in the Wugang Tong(0.5010).The high diversity of 4 goose breeds might lie in the traffic obstruction.The results of the heterozygosity were consistent with that of PIC.UPGMA tree was completed through analysis of D_A genetic distances.Daozhou Dark and Lingxian White were in the first group;Wugang Tong was in the second group and Xupu was in the third group.

通过选用31个多态性较好的微卫星标记,检测了湖南4个地方鹅品种的遗传多样性。利用等位基因频率计算出各群体的平均遗传杂合度(H)、多态信息含量(PIC)和群体间的DA遗传距离。结果表明,31个微卫星位点中有25个微卫星位点在4个鹅群体中的多态信息含量均为中度多态,可作为有效的遗传标记用于各鹅品种的遗传多样性和系统发生关系的分析。4个鹅品种中,平均杂合度范围是0.5010~0.6705,各鹅种的杂合度都较高,最高的是酃县白鹅(0.6705),最低的是武冈铜鹅,而且杂合度的高低与PIC值的大小体现了较高的一致性。对DA遗传距离的计算表明,用UPGMA法进行聚类分析,结果4个鹅品种被聚为3类:道州黑鹅与酃县白鹅聚为第1类;武冈铜鹅自聚为第2类;溆浦鹅自聚为第3类。

 
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