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黑大豆
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  black soybean
    Antiatherogenic Effect and Antioxidation Mechanism of Anthocyanins Extracted from Black Soybean Seed Coat
    黑大豆种皮花色苷抗动脉粥样硬化及其抗氧化机制研究
短句来源
    Estrogenic activity and its mechanism of ethanol extract from black soybean
    黑大豆乙醇提取物的雌激素样作用及其机制研究
短句来源
    Objective: To explore the estrogenic activity and its mechanism of ethanol extract from black soybean(BSE).
    目的:评价黑大豆乙醇提取物(black soybean ethanol extract,BSE)的雌激素样作用及其作用机制。
短句来源
    The results showed that the ranges of total antioxidant capacity (TAC), total phenolics and anthocyanin contents in 127 black soybean accessions were 0.44~3.56 mmol/g, 7.05~74.82mg/g and 0.22~1.87mg/g, respectively, displaying significant genotype difference.
    127份黑大豆种质按快速聚类法可聚成6大类群,分别由3、24、20、31、37和12份种质组成。 黑大豆的TAC与其总酚和花色苷含量之间存在显著的相关性,相关系数均达到极显著水平(P<0.01)。
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  black soybean
The results showed that ethanol extracts of Adzuki bean (100? μg/ml), Black soybean (1-200?μg/ml) and Lima bean (100?μg/ml), could strongly stimulated the proliferation of MCF-7 cells (P>amp;lt;0.01 vs.
      
Using RT-PCR measurement, it was found that ethanol extracts of Adzuki bean, Black soybean and Lima bean could induce pS2 transcription in MCF-7 cells.
      
The brown and black soybean seed coat contained much higher levels of phenolic compounds and anthocyanins in seed coat tissue than the yellow or green coat soybean.
      
Hydrolysis of black soybean isoflavone glycosides by Bacillus subtilis natto
      
Ground pork-fat patties were cooked alone (control), with NaCl solution, with NaCl solution containing sucrose, with fermented soybean broth, with fermented black soybean broth, and with commercial soy sauce products at 125°C for 2 h.
      


Objective: To explore the estrogenic activity and its mechanism of ethanol extract from black soybean(BSE).Method: The modified MCF-7 cell proliferation assay was used to evaluate the estrogenic activity of BSE.And the possible mechanisms were addressed using RT-PCR measurements in which pure estrogen receptor antagonist ICI182,780 was employed as a tool.Result: The estrogenic activity of BSE at various concentrations(1-1 000 μg·mL~(-1)),expressed as proliferative effect(PE) relative to that of solvent control,was...

Objective: To explore the estrogenic activity and its mechanism of ethanol extract from black soybean(BSE).Method: The modified MCF-7 cell proliferation assay was used to evaluate the estrogenic activity of BSE.And the possible mechanisms were addressed using RT-PCR measurements in which pure estrogen receptor antagonist ICI182,780 was employed as a tool.Result: The estrogenic activity of BSE at various concentrations(1-1 000 μg·mL~(-1)),expressed as proliferative effect(PE) relative to that of solvent control,was examined.The results indicated that,at low concentration range(10-200 μg·mL~(-1)),BSE was able to induce MCF-7 cell growth with a maximum at 100 μg·mL~(-1).The results of RT-PCR showed that pS_(2) and PR mRNA could be significantly induced by BSE in MCF-7 cells,which was relative to the concentration of BSE.Moreover,the specific estrogen receptor antagonist,ICI182,780 could block these reactions.Conclusion: Ethanol extract of black soybean can stimulate the growth of MCF-7 cells and increase the expression of estrogen receptor-responsive gene,suggesting that the estrogenic effects of BSE are mediated by the estrogen receptor.

目的:评价黑大豆乙醇提取物(black soybean ethanol extract,BSE)的雌激素样作用及其作用机制。方法:采用富含雌激素受体的雌激素依赖性人乳腺癌细胞株MCF-7细胞,分别以E-SCREEN法观察BSE对其增殖的影响、以RT-PCR法观察BSE对其雌激素效应基因mRNA表达的影响,并以雌激素受体拮抗剂ICI182,780为工具药来评价BSE发挥雌激素样作用的机制。结果:与溶剂对照组相比较,BSE(10~200μg.mL-1)能显著促进MCF-7细胞的增殖,100μg.mL-1时作用达到最大;BSE能明显诱导MCF-7细胞pS2,孕激素受体(PR)基因mRNA的表达,并与药物浓度有一定相关性;以上作用均能被雌激素受体拮抗剂所完全拮抗。结论:BSE能促进MCF-7细胞的增殖,诱导雌激素效应基因mRNA的表达,即BSE能通过雌激素受体发挥雌激素样作用。

 
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