Methods: The protein coding region of pc 1 cDNA was amplified using nested PCR technology from the primary cloning plasmid and subcloned into the eukaryotic expressing vectors pEGFP N1 and pCDNA3.1( )/myc his containing EGFP and MYC tag,respectively,then transfected into the human prostatic cancer cell line C4 2.The location of its expression product in C4 2 was observed with immunocytochemistry and laser confocal scanning microscopy.
To obtain a new member of the human prostate carcinoma tumor inducing gene ( PTI 1) family in human prostate carcinoma cell line PC 3, human PTI 1 cDNA was synthesized from human prostate carcinoma cell line PC 3 by RT PCR, which was then subcloned into pUC19 vector and sequenced.
Methods After treatment of 1×10 -9 ? mol/L for 24 h, expression of cell cycle regulation molecules including CDK2, CDK4, CDK6 and p27 kipl in human prostate carcinoma cell line LNCaP and PC 3 was detected by using immunoprecipitation and Western blot analysis after 0, 8 and 24 h of withdrawal of androgen.
To obtain a new member of the human prostate carcinoma tumor-inducing (PTI-1) gene family in human prostate carcinoma cell line PC-3. The human PTI-1 cDNA was synthesized from human prostatic carcinoma cell line PC-3 by RT-PCR, then subcloned into pUC19 vector and sequenced.
Following incubation, the cultured cells containing immature DCs were concentrated and transfected with tumour mRNA from human prostate cancer cell lines employing a highly efficient electroporation procedure.
Screening of differently expressed genes in human prostate cancer cell lines with different metastasis potentials
It was concluded that two subtractive libraries of human prostate cancer cell lines with different metastasis potentials were constructed successfully.
Effect of tumor necrosis factor-α and interferon-γ on the growth of human prostate cancer cell lines
Endopeptidase 24.11 activity in the human prostate cancer cell lines LNCaP and PPC-1
Normal genital skin fibroblasts (GSF) and the human prostate carcinoma cell line LNCaP have been used widely as cell culture models of genital origin to study androgen receptor (AR) signaling.
Antiproliferative effect of interferons on human prostate carcinoma cell lines
The effect of purified human fibroblast beta-interferon (B-IFN) and recombinant alpha-2b-interferon (A-IFN) on cell proliferation was investigated in two human prostate carcinoma cell lines, named PC-3 and DU-145.
High mannose-type glycans were sought on the human prostate carcinoma cell surface.
Results: BE-4-4-4-4 was shown to be approximately 4 to 86 times more cytotoxic in clonogenic assays than DENSPM in both rat and human prostate carcinoma cell lines.
Monoclonal antibody-defined antigens of human prostate cancer cell line PC3
Cell cycle perturbations and radiosensitization effects in a human prostate cancer cell line
Unchecked DNA synthesis and blocked cell division induced by methionine deprivation in a human prostate cancer cell line
In order to establish an animal model of bone metastasis, cells from a human prostate cancer cell line (PC-3) were injected into the tail veins of athymic nude mice while the inferior vena cava was occluded.
To evaluate the role of MKP-1 in regulating cell death and tumor growth in prostate cancer, MKP-1 was conditionally overexpressed in the human prostate cancer cell line DU145.