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   角质纤维 的翻译结果: 查询用时:0.207秒
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角质纤维
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     RESULTS: At the 7th and 14th day after osteoblastic induction, 11.5% and 37.5% of the cells stained with modified Gomori were positive, respectively.
     做神经元特异性烯醇化酶、角质纤维酸性蛋白、神经丝蛋白免疫组化染色 . 结果 :在加入成骨细胞分化培养液后 7和1 4d钙钴法碱性磷酸酶染色阳性细胞百分率分别为 1 1 .5 %和37.5 % .
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     Special Style Fibres
     特殊风格纤维
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     fibre trachieds;
     纤维管胞;
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     Definition of Fiber Hornification
     纤维角质化的表征
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AIM: To study the differentiating characteristics of rat mesenchymal stem cells in vitro . METHODS: The rat marrow stem cells were isolated by Percoll (1.073 kg/L). The third passage cells had been induced by 0.1 μmol/L dexamethasome, 10 mmol/L β glycerophosphate and 50 μmol/L ascorbic acid 2 phosphate. On the 7th day and 14th day, the cells were stained by modified Gomori to show the activities of alkaline phosphatase. On the 21st day, the cells were stained by Von Kassa to show calcium salts. The...

AIM: To study the differentiating characteristics of rat mesenchymal stem cells in vitro . METHODS: The rat marrow stem cells were isolated by Percoll (1.073 kg/L). The third passage cells had been induced by 0.1 μmol/L dexamethasome, 10 mmol/L β glycerophosphate and 50 μmol/L ascorbic acid 2 phosphate. On the 7th day and 14th day, the cells were stained by modified Gomori to show the activities of alkaline phosphatase. On the 21st day, the cells were stained by Von Kassa to show calcium salts. The MSCs were induced by 1 μmol/L Dexamethasome, 10 mg/L bovine insulin, 0.25 mmol/L 1 methyl 3 isobutylxamthine and 100 μmol/L indomethacin. The MSCs were induced by 5 mmol/L β mercaptoethanol for 5 h and analyzed by immunohistochemistry. RESULTS: At the 7th and 14th day after osteoblastic induction, 11.5% and 37.5% of the cells stained with modified Gomori were positive, respectively. At the 21st day, the calcium salts were observed by Von Kassa. After 3 and 7 days induction, the positive cells stained by Oil Red O were 22% and 90.33%, respectively. After a 5 hour β mercaptoethanol induction of MSCs, immunohistochemistry showed that the positive rates of NSE and NF 200 were 33% and 37.5%, and GFAP was negative. CONCLUSION: MSCs can differentiate into osteroblast, adipocyte and neur like cells.

目的 :研究大鼠骨髓间充质干细胞体外分化特性 .方法 :用密度为 1 .0 73kg/L的细胞分离液分离骨髓间充质干细胞 .取第 3代培养细胞用含 0 .1 μmol/L地塞米松、1 0mmol/Lβ 甘油磷酸钠、5 0 μmol/L维生素C的成骨细胞分化培养液培养 .于培养 7,1 4d取细胞爬片做改良的Gomori钙钴法碱性磷酸酶染色 ,2 1d做VonKassa染色 ;脂肪细胞分化培养用含 1 0mg/L胰岛素、1 μmol/L地塞米松、0 .2 5mmol/L 1 甲基 3 异丁基黄嘌呤和 1 0 0 μmol/L吲哚美辛的培养液诱导培养 ,于诱导 3,7d取细胞爬片做油红O染色 .用含 1mmol/Lβ 巯基乙醇和 1 5 0mL/LFBS的DMEM培养基先预诱导分化 2 4h .再用含有 5mmol/Lβ 巯基乙醇的DMEM无血清培养基诱导分化5h .做神经元特异性烯醇化酶、角质纤维酸性蛋白、神经丝蛋白免疫组化染色 .结果 :在加入成骨细胞分化培养液后 7和1 4d钙钴法碱性磷酸酶染色阳性细胞百分率分别为 1 1 .5 %和37.5 % .2 1d的细胞爬片VonKassa染色显示钙...

目的 :研究大鼠骨髓间充质干细胞体外分化特性 .方法 :用密度为 1 .0 73kg/L的细胞分离液分离骨髓间充质干细胞 .取第 3代培养细胞用含 0 .1 μmol/L地塞米松、1 0mmol/Lβ 甘油磷酸钠、5 0 μmol/L维生素C的成骨细胞分化培养液培养 .于培养 7,1 4d取细胞爬片做改良的Gomori钙钴法碱性磷酸酶染色 ,2 1d做VonKassa染色 ;脂肪细胞分化培养用含 1 0mg/L胰岛素、1 μmol/L地塞米松、0 .2 5mmol/L 1 甲基 3 异丁基黄嘌呤和 1 0 0 μmol/L吲哚美辛的培养液诱导培养 ,于诱导 3,7d取细胞爬片做油红O染色 .用含 1mmol/Lβ 巯基乙醇和 1 5 0mL/LFBS的DMEM培养基先预诱导分化 2 4h .再用含有 5mmol/Lβ 巯基乙醇的DMEM无血清培养基诱导分化5h .做神经元特异性烯醇化酶、角质纤维酸性蛋白、神经丝蛋白免疫组化染色 .结果 :在加入成骨细胞分化培养液后 7和1 4d钙钴法碱性磷酸酶染色阳性细胞百分率分别为 1 1 .5 %和37.5 % .2 1d的细胞爬片VonKassa染色显示钙盐沉积 .在脂肪细胞诱导 3和 7d分别得到 2 2 %和 90 .33%油红O染色阳性细胞 .5h神经细胞诱导分化后NSE免疫组化染色阳性率为 33% ,NF 2 0 0阳性细胞率为 37.5 % ,GFAP染色阴性 .结论 :骨髓间充质干细胞在体外可定向加药诱导分化为成骨细胞、脂肪细胞和神经元样

 
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