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限制培养基
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  limiting medium
     Escherichia coli (E. coli) K-- 12 IAM 1264 cells were synchronized by a modified version of phosphate starvation described by Kepes and Kepes. The cells which were obtained by repeating 8 cycles in synchronization step lasted for two or three cycles of synchrony under a free growth in phosphate non--limiting medium.
     用改良的Kepes磷酸饥饿法诱导大肠杆菌K—12AM1264同步化生长,细胞经8轮同步化步骤后可在磷酸不限制培养基中自由生长保持2~3次同步化细胞周期。
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  “限制培养基”译为未确定词的双语例句
     Aeromonas hydrophila could not produce detectable siderophore when being incubated at iron limited medium (M70-Fe) and was analyzed by the chrome azurol S (CAS) method.
     嗜水气单胞菌(Aeromonashydrophila)在铁限制培养基(M70 Fe)中培养,并用铬天青S(CAS)方法分析,不能产生可检测的含铁细胞;
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     To increase the production of ligninolytic enzymes, the present work studied the influence of glucose feeding on ligninolytic enzyme production of P.chrysosporium in nitrogen limited (C/N ratio is 56.0/8.8) medium.
     为了提高木质素降解酶的产量,采用氮限制培养基(C/N=56.0/8.8),研究了葡萄糖补料对P. chrysosporium产木质素降解酶的影响.
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  相似匹配句对
     The composition of culture medium
     培养基的组成
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     Restrictions on the Right of Trade Mark
     论商标权的限制
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     Furthermore,the optimum enrichment medium of L.b-S1 were screened by orthogonality test.
     b-S1的增殖培养基
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     The Restriction of The Peotics of Culture
     文化诗学的限制
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     So the medium for conservation of strawberry in vitro should contain proper concentration of nutrient substance to limit the plantlet growing.
     草莓试管苗保存培养基应选择适宜浓度的营养物质来限制试管苗的生长。
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  limiting medium
putida 5-x cultured in sulfate-limiting medium reached maximum during the late stationary growth phase or early death phase, and reached minimum during the log growth phase.
      
Growth of Streptococcus mutans in an iron-limiting medium
      
In citrate limiting medium the esterase activity of Aspergillus niger had a maximum value at the lowest dilution rate (D=0.013 h-1) and at all higher dilution rates progressively decreased in activity.
      
In glucose limiting medium the esterase activity values were always lower than in citrate limiting medium and did not show much variation with varying dilution rate.
      
Electrophoresis of cell free extracts from all dilution rates revealed a multimolecular esterase profile only at D=0.013 h-1 in citrate limiting medium, which was also the only dilution rate to support good conidiation.
      
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Mydecamycin significantly inhibits the growth of Bacillus subtilis BF 7658.

麦迪霉素(Mydecamycin)明显地抑制枯草杆菌BF7658的生长。在无机磷酸的含量受到限制的培养基中生长的枯草杆菌BF7658的细胞,能迅速地诱导生成碱性磷酸酶。在碱性磷酸酶诱导合成的过程中,加进0.5微克/毫升的麦迪加霉素,该酶的合成几乎完全被阻遏。在碱性磷酸酶催化腺苷酸(AMP)水解的反应液中,即使加进100微克/毫升的麦迪霉素,该酶的催化活性也不受抑制。

Escherichia coli (E. coli) K-- 12 IAM 1264 cells were synchronized by a modified version of phosphate starvation described by Kepes and Kepes. The cells which were obtained by repeating 8 cycles in synchronization step lasted for two or three cycles of synchrony under a free growth in phosphate non--limiting medium. The doubling time and the cell division period of this E. coli were 55 and 15 min, respecitively. The cell cycle was divided into three periods, cell division period (P ), the period between cell...

Escherichia coli (E. coli) K-- 12 IAM 1264 cells were synchronized by a modified version of phosphate starvation described by Kepes and Kepes. The cells which were obtained by repeating 8 cycles in synchronization step lasted for two or three cycles of synchrony under a free growth in phosphate non--limiting medium. The doubling time and the cell division period of this E. coli were 55 and 15 min, respecitively. The cell cycle was divided into three periods, cell division period (P ), the period between cell division and initiation of chromosome replication (Q ) and the period between initiation of chromosome replication and cell division (R). The R period was subdivided into two periods, R1 in which the rate of thymidine uptake into DNA was increasing,and R2 in which it was constant. The periods of R1 and R2 were 15 and 10 min, respectively.

用改良的Kepes磷酸饥饿法诱导大肠杆菌K—12AM1264同步化生长,细胞经8轮同步化步骤后可在磷酸不限制培养基中自由生长保持2~3次同步化细胞周期。K—12AM1264大肠杆菌的细胞增倍和分化周期分别为55和15min,同步化细胞周期可分达3个时相。细胞分裂期(P),细胞分裂和染色体复制起始间期(Q)以及染色体复制起始和细胞分裂间期(R)。R期又可分R1和R2两个亚期。在R1亚期胸腺呼啶掺入DNA的速度增加,在R2亚期掺入速度保持恒定。R1和R2期分别为15和10min。

Objective To isolate neural precursor cells from human embryonic cortical tissue of 20 weeks and identify their proliferation capacity as well as differentiating potential. Methods Human fetuses of about 20 weeks from spontaneous abortion were adopted. A serum free medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) was used to make the neural precursor cell divide continuously in the culture. The proliferating ability of these cells was fested with BrdU. Growth factors...

Objective To isolate neural precursor cells from human embryonic cortical tissue of 20 weeks and identify their proliferation capacity as well as differentiating potential. Methods Human fetuses of about 20 weeks from spontaneous abortion were adopted. A serum free medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) was used to make the neural precursor cell divide continuously in the culture. The proliferating ability of these cells was fested with BrdU. Growth factors were remored so as to induce differentiation of the precursor cells. The cell cycle of the cells in the neurosphere was analysed using flow cytometry. Results Cells from human embryonic cortical tissue could be maintained and propagated in the presence of growth factors. Neurospheres generated continually and the cells in the sphere could incorporate into BrdU. Upon differentiation, the precursor cells gave rise to mature neurons, astrocytes and oligodendrocytes. They retained their multilineage potential over repeated passages.The cell cycle analysis indicated that the cells proliferated actively. Conclusion Cells that able to self renew and differentiate into mature cells in culture cam be isolated from human embryonic tissues. They are werified to be neural precursor cells.

目的 从 2 0周人胚皮层中分离神经前体细胞并鉴定其增殖及分化能力。方法 应用包含碱性成纤维细胞生长因子 (bFGF)和表皮细胞生长因子 (EGF)的无血清限制培养基从 2 0周自然流产人胚胎皮层组织中分离神经前体细胞 ,通过神经球形成法使其不断增殖 ,利用BrdU检测细胞的增殖能力 ,去除生长因子后诱导神经前体细胞分化。利用流式细胞仪检测神经球内细胞的增殖细胞周期。结果 人胚胎皮层存在神经前体细胞 ,这些细胞于EGF及bFGF作用下可不断增殖形成神经球 ,并能自我更新和结合BrdU ,于生长因子去除后能够分化成熟的神经元、星形胶质细胞和少突胶质细胞。反复传代后这些细胞可保持其增殖及分化能力。细胞周期结果显示神经球内细胞增殖较为活跃。结论 从人胚胎中分离的细胞能于体外自我更新和分化为成熟的细胞 ,证实为神经前体细胞。

 
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