The paper deals with the lactate dehydrogenase (LDH) and esterase (EST) isoenzymes of queen bee, spado, drone and larvae with two different sizes of Italian honeybee (Apis mellifera) by using polyacrylamide gel electrophoresis and optical density scanning.
The results show that:queen bee larvae developed better under the condition of relative humidity 90%±2%and temperature 35.5±0.1℃ than the temperature 34.5±0.1℃,and larvae could benefit from fresh pure royal jelly added with alittle honey.
In the developmental stages of queen bees from larvae of 4 days age to sexmatutity the contents of E2, T, FSH and LH were always much higher than that in other stages. From emerging period to sexmaturity the contents of E2, T and LH went up significantly.
The results indicated: ① when larvae of workerbees and queen bees were compared at the stage of 4~5 days old, the content of E2 in the former was significantly lower than that in the latter. At this stage the content of T was significantly higher than that of E2 in larvae of worker bees, while in larvae of queen bees the very contrary was the case.
The contents of T and E2 in royal jelly were significantly higher than that in larvae of queen bees, while the content of FSH was significantly lower. The content of E2 in the royal jelly fed larvae of queen bees at different stages was always significantly higher than that of T.
The results show that whenthe colonies are fed by pure pollen and pollen adding 20% bean powder, the weight of workers bred by those have no dif-ference. The weight of queen bees have greater difference and that of drones have greatest difference.
Because reduction of honey yield also may be affected by other factors, such as location of the hives relative to a food source and vigor of the queen bee, the final assessment of honey yield was complicated.
A queen bee extract (Apis mellifera L.) (Hymenoptera) reduces the fecundity ofTenebrio molitor L.
3 different doses of crude queen bee extract were administered to pairs of adultTenebrio molitor L.; the extract exerted an inhibitory action on fecundity, but did not induce complete sterility of the females.
1.The reproductive organs fixed in picro-alcohol, according to the formula of the author, have yielded equally good results as with picro-formol.2.Each ovary of the queen bee consists of 73 polytrophic ovarioles.
The ovary of the newly emerged queen bee is small in size and does not show mature stages of the oocyte.
We examined the effects of synthetic honey bee queen mandibular pheromone (QMP), four newly identified queen retinue pheromone components, and whole-queen extracts on the ovary development of caged worker bees.
Also the multiple mating system of the honey bee queen supports the propagation of alleles causing reproductive dominance of workers.
Production and transmission of honey bee queen (Apis mellifera L.) mandibular gland pheromone
Sperm usage was investigated in a naturally mated honey bee queen.
Queen substances from the abdomen of the honey bee queen