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观察型
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    AN UNDERICE OPERATION OF SJT-5 ROV
    SJT-5观察型潜水器冰下作业
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    In this research, a visual mold is utilized to directly and dynamically observe the filling process as well as generation process of molding defects.
    本研究采用可视化方法,通过可视化技术直接观察型腔内树脂流动过程,以及各种注射成型缺陷的产生过程,发现加工成型过程中的某些未知现象。
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    Methods:Collagen type Ⅰ and Ⅲ in OSF were investigated by immunohistochemical examination and image pattern analysis.
    方法:应用免疫组织化学方法观察型及型胶原在OSF中的分布,并应用图像分析仪对正常颊粘膜及OSF各期中的、型胶原进行定量分析。
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    Methods The expression of type Ⅰ collagen and integrin α 2β 1 in the primary, sixth and fifteenth passage of osteoblasts were detected by S P immunohistological staining technique, and their mRNA expression by quantity RT PCR technique.
    方法 选用原代、第 6代及第 15代人胚骨膜成骨细胞 ,S- P免疫组织化学染色观察 型胶原及整合素α2 β1 的表达情况 ,定量 RT- PCR技术检测 型胶原及整合素α2 β1 的 m RNA表达情况。
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    In renal cortex, zymography was used to investigate the gelatinolytic activity of MMP 2. Western Blot was used to evalulate the content of TIMP 2, and Northern Blot was used to observe the gene expression of MMP 2 and TIMP 2. Results In renal cortex from the type 2 diabetic rats, type Ⅳ collagen staining in glomeruli was increased at the 12th and 20th week;
    方法 利用免疫组织化学观察 型胶原在肾小球的表达 ,酶谱法测定肾皮质MMP- 2的活性 ,蛋白印迹法检测肾皮质 TIMP- 2的含量 ,Northern Blot观察肾皮质 MMP- 2和 TIMP- 2的基因表达。 结果 类似人类 2型糖尿病大鼠肾小球 型胶原成分表达增强 ;
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  observed pattern
The observed pattern of genetic differentiation was probably caused largely by genetic drift under the conditions of a limited gene flow because of homing (which is typical of the Dolly Varden) and the presence of isolated nonanadromous populations.
      
We show that most of the peculiar manifestations of this object in the hard spectral range and the observed pattern of mass transfer in the system can be explained in terms of a model in which the state of the white dwarf is classified as an ejector.
      
The observed pattern of variations in the fluxes and radial velocities of the features can be explained by the presence of inhomogeneities in the medium, which can form elongated structures like filaments or chains.
      
The well-known model that attributes the formation of a bipolar sunspot group to the emergence of a flux tube disagrees sharply with the usual observed pattern of phenomena.
      
Furthermore, the observed pattern of the photospheric velocities shows periodic motions (with a period of 5 min) directed from the inner boundary of the penumbra and superpenumbra toward the line of maximum Evershed velocity.
      
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The Effects of time factors on the compacting process of foundry sands were seldom mentioned and reported. In this paper, the influence of different squeeze acting time on the deformation of moulding sands was studied. Properties of moulding sandsrelated to its visco-properties, such as creep, spring-back and deformation under recompaction were investigated. Equations for creep of moulding sands were obtained. With comparison tests, it was given that the creep and springback of foundry sands during compacting...

The Effects of time factors on the compacting process of foundry sands were seldom mentioned and reported. In this paper, the influence of different squeeze acting time on the deformation of moulding sands was studied. Properties of moulding sandsrelated to its visco-properties, such as creep, spring-back and deformation under recompaction were investigated. Equations for creep of moulding sands were obtained. With comparison tests, it was given that the creep and springback of foundry sands during compacting are caused chiefly by the deformation and visco-elastic properties of the betonit thin film on the contact points between sand grains and not by the sand grains themselves. Some concepts of rheology were introduced to analyse those properties of sands and some conclusions were drawn out.

型砂紧实过程中的时间因素的影响,以前很少有人研究过。本文经过实际试验观察了型砂在保压时间不同时变形情况的变化,研究了型砂的蠕变、回弹、再紧实变形等与型砂的粘弹性和粘塑性有关的变形,提出了型砂蠕变方程式。通过对比试验,得出型砂的这些变形主要是由于砂粒接触点上粘土膜的变形和粘弹性,而不是由于砂粒本身的变形。文中用流变学的概念分析型砂的这些性能,并得出了有关型砂紧实机理的一些结论。

The plaque character and temperature sensitivity of herpes simplex virus type 1 ( HSV-1 ) and type 2 (HSV-2) were studied.The plaques formed by HSV-2 ( 3.27±0.33mm) in guinea pig embryo ( GPE ) cells were bigger than those by HSV-1 ( 1.79±0.24mm ) 3 days postinoculation. Also, the plaque margin of HSV-2 was neat,but that of HSV-1,non-neat. Although no difference of themosensitivity to 37℃ waterbath was found between HSV-1 and HSV-2, HSV-1 was more resistant to low temperature storage (-70℃) than HSV-2.After...

The plaque character and temperature sensitivity of herpes simplex virus type 1 ( HSV-1 ) and type 2 (HSV-2) were studied.The plaques formed by HSV-2 ( 3.27±0.33mm) in guinea pig embryo ( GPE ) cells were bigger than those by HSV-1 ( 1.79±0.24mm ) 3 days postinoculation. Also, the plaque margin of HSV-2 was neat,but that of HSV-1,non-neat. Although no difference of themosensitivity to 37℃ waterbath was found between HSV-1 and HSV-2, HSV-1 was more resistant to low temperature storage (-70℃) than HSV-2.After EcoRI digestion, HSV-1 DNA showed five well-separated bands and only two HSV-2 DNA bands were found in the electrophoretic patterns. Moreover, over eight well-separated bands were shown in the HpaⅠ cleaved HSV-1 DNA,whereas only one band was clearly visible in the HapⅠ cleaved HSV-2 DNA.

本实验观察了Ⅰ型和Ⅱ型单纯疱疹病毒(简称HSV-1和HSV-2)的空斑特征和对温度的敏感性。病毒接种豚鼠胚细胞3天后,HSV-2形成的空斑较HSV-1的大,边缘也较整齐。两者对热(37℃水浴)灭活无明显差别,但HSV-2对低温(-70℃)的耐受性比HSV-1弱。HSV-1 DNA经EcoRI消化后,在我们的实验条件下,凝胶电泳可分辨出5条清晰的DNA带,而在此分子量范围内,HSV-2仅有两条带;用Hpa Ⅰ消化后,HSV-1有8条以上分辨清晰的带,HSV-2仅有一条可分辨的带。 本研究为HSV-1和HSV-2的分型鉴定、保藏提供了简便、准确的检测手段和实验依据。

16 rat lungs were evaluated by cytologic studies after intra-tracheally instillation with 40mg of silica crystals at day 0, 15, 30 and 51~54 since then. The lavaged fluid was used for motility assay and as a source of macrophages. After enzymatic dissociation of cells in the whole washed lung, the free cells from the lungs including the type 2 pneumocytes were obtained. After the first overnight incubation was needed to get the type 2 pneumocytes for the motility assay as well as the type 2 cell supernatant....

16 rat lungs were evaluated by cytologic studies after intra-tracheally instillation with 40mg of silica crystals at day 0, 15, 30 and 51~54 since then. The lavaged fluid was used for motility assay and as a source of macrophages. After enzymatic dissociation of cells in the whole washed lung, the free cells from the lungs including the type 2 pneumocytes were obtained. After the first overnight incubation was needed to get the type 2 pneumocytes for the motility assay as well as the type 2 cell supernatant. In both the Agarose assay and Boyden chamber assay. The alveolar macrophage appeared to migrate toward the type 2 cell supernatant in greater extent (at 15, 30 day, P<0.01) than toward the serum free RPMI-1640, supernatant of fibroblast and silica.So, it was considered that the accumulation of macrophages in the silicotic rat lungs might be related to the action of the type 2 cells since the cultured type 2 pneumocytes supernatants might stimulate macrophage motility as chemotaxis.

大白鼠实验性矽肺分别于染尘后15~54天内处死,取离体肺作气管—肺冲洗获取肺泡巨噬细胞后,全肺酶化切碎获取肺Ⅱ型上皮,经短时培养获取其上清液,体外用琼脂糖滴与Boyden室观察Ⅱ型上皮上清液对肺泡巨噬细胞的趋化作用。结果指出,染尘后的第15、30以及54天的肺Ⅱ型上皮上清液对肺泡巨噬细胞均有明显的趋化作用。

 
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