The effects of antisense oligonucleotide for c-myc, antisense eukaryotic expression vectors for c-myc and antisense recombinant adenoviral vector for c-myc on the proliferation of rat airway smooth muscle cells
The recombinant plasmids pcDNA3.1+/flk-l(n1-4) wasconstructed and transfected into eucaryotic expression system COS-7 cells byLipofectamine. Then detecting protein expression of flk-l(n1-4) by Western blot.
2(1) LRP16 eucaryotic expression vector (pcDNA3.1-LRP16) was constructed and transfected into ALVA41 and DU145 cells with the control pcDNA3.1(+) vector, respectively, and then four groups of cells expressed LRP16 gene or empty vector (A16, A3.1, D16, D3.1) were selected by G418 and cultured for further study;
Both sense and antisense clones permanently incorporated mammalian expression vectors into their genomes.
This has resulted in the development of baculoviruses as mammalian expression systems and even as vestors for gene therapy.
Recently, we demonstrated that pUMVC3-hFLex, an active plasmid, mammalian expression vector for the secretion of Flt3-L, reversed established airway hyperresponsiveness (AHR) in a murine model of acute allergic airway inflammation.
Cloning of cDNAs from a mammalian expression library by a direct selection-amplification method
A sensitive method was devised for cloning cDNAs from a mammalian expression library based on singlecell detection and selection of transfected cells.
The HSV1 gD gene (pDNAgD) served as an immunogen; mTNFα or its gene cloned in an eukaryotic expression vector (pDNAmTNF) were used to modulate the immune response.
In this report, an eukaryotic expression plasmid pEGFP-C1-RA28 was constructed and transfected with parental cell line GLC-82 to analyze protein expression and its distribution in living cells.
To observe its antiviral effect against Hepatitis B virus (HBV) and Coxsackie virus B3 (CVB3), we constructed an eukaryotic expression vector of human GBP-1(hGBP-1).
Methods: Functional part of M-CSF cDNA was inserted into an eukaryotic expression plasmid pCMV/myc/nuc, which can add three NLS to the C-terminal of the expressed protein and direct the protein into the cell nuclei.
Methods: An eukaryotic expression vector pEGFP/hDaxx, which could express a fusion protein GFP-Daxx, was transfected into macrophages.