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真核表达     
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  eukaryotic expression
     Construction and eukaryotic expression of anti-LPS Fab,BPI and Fab-BPI fusion protein
     抗LPS Fab、BPI和Fab-BPI融合蛋白真核表达载体的构建及其在CHO细胞中的表达
短句来源
     The effects of antisense oligonucleotide for c-myc, antisense eukaryotic expression vectors for c-myc and antisense recombinant adenoviral vector for c-myc on the proliferation of rat airway smooth muscle cells
     反义c-myc寡核苷酸、反义c-myc真核表达载体和反义c-myc重组腺病毒载体在大鼠气道平滑肌细胞增殖中的作用
短句来源
     Cloning of Suilysin Gene of Streptococcus Suis and Its Prokaryotic and Eukaryotic Expression
     猪链球菌溶血素基因的克隆及其原核和真核表达
短句来源
     siRNAs Interference the Nanog Gene of ES Cells and the Eukaryotic Expression Vector Construction of Nanog
     siRNA干扰ES细胞nanog基因及nanog基因真核表达载体的构建
短句来源
     Construction of Eukaryotic Expression Vector of SiRNA Specific for BCR/ABL Fusion Gene and Its Effects on K562 Cells
     BCR/ABL特异性siRNA真核表达载体构建及其对K562细胞的影响
短句来源
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  mammalian expression
     Methods: (1) Construction of antisense hTOP3 mammalian expression vector pcDNA3.0-antihTOP3;
     方法:(1)构建hTOP3的反义真核表达载体pcDNA3.0-antihTOP3;
短句来源
     Cloning of Xeroderma Pigmentosum Complementation Group B (XPB) Gene and Construction of Mammalian Expression Recombinant pcDNA3.1(+)-XPB
     人XPB基因cDNA克隆及真核表达重组体pcDNA3.1(+)-XPB的构建
短句来源
     Cloning of Xeroderma Pigmentosum Complementation Group D (XPD) Gene and Construction of Mammalian Expression Recombinant pEGFP-N2/XPD
     人XPD基因的cDNA克隆及真核表达载体pEGFP-N2/XPD的构建
短句来源
     Methods:1: Construct a mammalian expression vector pcDNA3.1/myc-His(-)C-bFGF with gene cloning technique.
     一:通过基因克隆技术,构建并大量制备pcDNA3.1/myc-His(-)C-bFGF真核表达体系;
短句来源
     Methods:Construct a mammalian expression vector PCMV-MCS-VEGF-IRES2-EGFP.
     方法:构建PCMV-MCS-VEGF-IRES2-EGFP真核表达载体。
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  eucaryotic expression
     Construction of Human Parvovirus B19-VP2 Gene in Eucaryotic Expression of Plasmid
     人微小病毒B19-VP2基因真核表达质粒的构建
短句来源
     The recombinant plasmids pcDNA3.1+/flk-l(n1-4) wasconstructed and transfected into eucaryotic expression system COS-7 cells byLipofectamine. Then detecting protein expression of flk-l(n1-4) by Western blot.
     并经脂质体法将重组质粒pcDNA3.1+/flk-1_((n1-4))转染真核表达系统COS-7细胞,通过Western blot实验检测重组质粒pcDNA3.1+/flk-1_((n1-4))在真核表达系统COS-7细胞中蛋白的表达。
短句来源
     Construction of eucaryotic expression vector of T-cell receptor γV_1 and its expression
     pcDNA3/TCRγV_1真核表达载体的构建及表达
短句来源
     2(1) LRP16 eucaryotic expression vector (pcDNA3.1-LRP16) was constructed and transfected into ALVA41 and DU145 cells with the control pcDNA3.1(+) vector, respectively, and then four groups of cells expressed LRP16 gene or empty vector (A16, A3.1, D16, D3.1) were selected by G418 and cultured for further study;
     2.(1)构建LRPl6真核表达载体(pcDNA3.1-LRP16)并与对照空载体(pcDNA3.1)分别转染ALVA41和DU145细胞,将G418筛选获得的表达外源LRP16基因以及空载体的四组细胞(A16、A3.1、D16、D3.1)继续传代培养。
短句来源
     Methods:Four mutants of PKCa developed by PCR method were combined with pEGFP - N 1by DNA ligase and transferred into plasmids to construct eucaryotic expression vectors of PKC mutant - EGFP.
     方法:用PCR的方法构建了4个PKCα突变体,在DNA连接酶的作用下与pEGFP-N1结合后转移至质粒中,构建PKCα突变体-EGFP真核表达载体。
短句来源
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  an eukaryotic expression
     AIM: To construct an eukaryotic expression vector of CD80-IgG1 Fc, and to express the fusion protein in CHO cells.
     目的构建人CD80-IgG1Fc段融合蛋白的真核表达载体CD80-IgG1Fc/pcDNA3.1(+),并在CHO细胞中进行表达。
短句来源
     Construction of an eukaryotic expression vector containing IBDV VP2/4/3 and ChIL-2 fusion gene and their expression in Vero cells.
     IBDV VP2/4/3-ChIL-2融合基因真核表达载体的构建及其在Vero细胞中的表达
短句来源
     Construction of an Eukaryotic Expression Vector for SARS-CoV Sars7a and EGFP Fusion Protein Expression
     SARS-CoV Sars7a和EGFP融合蛋白真核表达载体构建及其表达
短句来源
     The construction of an eukaryotic expression plasmid encoding Toxoplasma gondii multi-antigenic gene P30,P22 and A_2/B genes of Cholera Toxin
     弓形虫表面抗原P30、P22与霍乱毒素A_2/B复合基因真核表达质粒的构建
短句来源
     After confirmed by DNA sequencing, the α1,3 galactosyltransferase gene was then subcloned into an eukaryotic expression vector pcDNA3.1/V5 HisA.
     经DNA测序证实序列无误后 ,将α1,3 半乳糖基转移酶基因片段亚克隆入pcDNA3.1/V5 HisA真核表达载体 .
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  eukaryotic expression
A recombinant strain of Salmonella choleraesuis C500, containing a eukaryotic expression plasmid pBO1 with the immune-dominant epitope of foot-and-mouth disease virus, was constructed.
      
The recombinant Sj14-3-3 of eukaryotic expression in Pichia pastoris was successfully harvested.
      
Eukaryotic expression vectors and immunoconjugates for cancer therapy
      
These include transcriptional activation of tissue-and tumor-specific promoters in eukaryotic expression vectors and use of antitumor-lirected immunoconjugates.
      
In addition, the soluble KIR2DL4 extracellular domain used in many studies was produced by eukaryotic expression, which is less efficient than prokaryotic expression.
      
更多          
  mammalian expression
Both sense and antisense clones permanently incorporated mammalian expression vectors into their genomes.
      
This has resulted in the development of baculoviruses as mammalian expression systems and even as vestors for gene therapy.
      
Recently, we demonstrated that pUMVC3-hFLex, an active plasmid, mammalian expression vector for the secretion of Flt3-L, reversed established airway hyperresponsiveness (AHR) in a murine model of acute allergic airway inflammation.
      
Cloning of cDNAs from a mammalian expression library by a direct selection-amplification method
      
A sensitive method was devised for cloning cDNAs from a mammalian expression library based on singlecell detection and selection of transfected cells.
      
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  eucaryotic expression
The eucaryotic expression plasmids were constructed to express the complete (HCc191) or the truncated (HCc69 and HCc40) HCV core genes, solely or fused with the HBV core gene (HBc144).
      
Thereafter, this epitope was incorporated into a eucaryotic expression plasmid containing a previously defined hepatitis delta virus epitope for protein tagging.
      
The entirevpr gene of human immunodeficiency virus type 1 (HIV-1) was cloned into procaryotic and eucaryotic expression vectors.
      
Budding of 100-160 nm p 55 core particles, resembling immature HIV-virions, was observed in the eucaryotic expression systems only.
      
Familiarity with procaryotic and eucaryotic expression systems and vector construction is desirable.
      
更多          
  an eukaryotic expression
The HSV1 gD gene (pDNAgD) served as an immunogen; mTNFα or its gene cloned in an eukaryotic expression vector (pDNAmTNF) were used to modulate the immune response.
      
In this report, an eukaryotic expression plasmid pEGFP-C1-RA28 was constructed and transfected with parental cell line GLC-82 to analyze protein expression and its distribution in living cells.
      
To observe its antiviral effect against Hepatitis B virus (HBV) and Coxsackie virus B3 (CVB3), we constructed an eukaryotic expression vector of human GBP-1(hGBP-1).
      
Methods: Functional part of M-CSF cDNA was inserted into an eukaryotic expression plasmid pCMV/myc/nuc, which can add three NLS to the C-terminal of the expressed protein and direct the protein into the cell nuclei.
      
Methods: An eukaryotic expression vector pEGFP/hDaxx, which could express a fusion protein GFP-Daxx, was transfected into macrophages.
      
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