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初级代谢
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  primary metabolite
     Nisin production by Streptococcus lactis SM526 was studied in batch fermentation process using a complex medium. Nisin production showed primary metabolite kinetics:nisin biosynthesis took place during the active growth phase and completely stopped when cells entered the stationary phase.
     利用分批培养,对乳酸链球菌SM526产生乳链菌肽(nisin)的代谢调控及发酵条件进行研究,代谢动力学分析表明,nisin在SM526指数生长期产生,到指数生长后期达到高峰,进入稳定期nisin逐渐停止产生,表现出一种初级代谢动力学特征。
短句来源
     Nisin production showed primary metabolite kinetics.
     乳链菌肽的合成表现为初级代谢动力学特征.
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  primary metabolism
     After function annotation,the most prevalent category was energy and primary metabolism which accounted for 32.5%,genes related with photosynthesis showed the highest frequency;
     对功能已知的contigs分类,能量和初级代谢类占32.5%,其中与光合作用相关的基因出现频率最高;
短句来源
     According to the putative function analysis, the 50 ESTs from Brassica napus were related to the primary metabolism, energy metabolism, secondary metabolism, disease defence, cell structure, transcription, protein synthesis, and signal transduction. The transcription products involved in the oxgen burst and jasnonade pathway and related metabolism pathway.
     从其功能看,50个ESTs序列分属于初级代谢、能量代谢、次生代谢、抗病防御、细胞结构、转录因子、蛋白质合成、信号转导等生命过程,关系到抗病反应过程包括参与氧爆发过程、茉莉酸途径,细胞保护和抗病防御有关代谢反应途径等。
短句来源
     Lignolytic degradation with fungi is a secondary metabolic activity, and in contrast to bacteria, it happens in primary metabolism.
     腐霉的降解作用发生在次级代谢阶段,而细菌发生在初级代谢阶段。
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     The primary problem that metabolic engineering should resolve is to restructure the metabolic flux in some pathways or to redirect the flux distributions in different pathways. The target is to trim the primary metabolism, to introduce the metabolic flux into the flux of end product.
     代谢工程解决的主要问题就是改变某些途径中的碳架物质流量或改变碳架物质流在不同途径中的流量分布,其目标就是修饰初级代谢,将碳架物质流导入目的产物的载流途径,以获得产物的最大转化率.
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     Phenylalanine ammonia-lyase (PAL) is the key enzyme that catalyzes the first reaction in phenylpropanoid pathway and links primary metabolism to phenylpropanoid metabolism.
     苯丙氨酸解氨酶(phenylalanineammonia-lyase,PAL)是连接植物初级代谢和苯丙烷类代谢、催化苯丙烷类代谢第一步反应的酶。
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  “初级代谢”译为未确定词的双语例句
     The optimum temperature and pH was 35℃ and 7.0-7.5 respectively. The optimum inoculation amount was 5%-6%. In addition,the shaking culture was more proper for nisin production than still culture.
     Nisin的产生显示了初级代谢动力学特征 ,发酵的最适温度是 35℃ ,培养基最适初始 pH为 7.0 - 7.5 ,最适接种量 5 %~ 6 % ,振荡培养的效价稍高于静止培养。
短句来源
     Thus,the correlations between ATP and spiramyc-ia synthesis and other variables in the course of fermentation were studied. It was found that high level of ATP in the culture broth was unfavorable to spiramycin biosynthesis.
     为此,本文研究了发酵过程中ATP的变化与螺旋霉素生物合成的关系,从中发现,过程ATP水平过高,对SPM生物合成不利,因基质主要用于初级代谢
短句来源
     The kinetics of cell growth and nisin production during culture of the strain was also explored.
     发酵动力学分析表明,该菌株产乳链菌肽表现出初级代谢动力学特征。
短句来源
     GSTs appear to have many different functions in plants in primary and secondary metabolism, in stress tolerance and in cell signaling.
     GST在植物的初级代谢和二级代谢、胁迫耐受、细胞信号等方面行使功能。
短句来源
     Cell growth and fermentation conditions of yield strain Streptococcus lactis P-99 were studied. Results showed that nisin production was synchronous with cell growth.
     文章对天然生物防腐剂乳链菌肽产生菌——乳链球菌P-99的摇瓶细胞生长和发酵条件进行了研究,发现该菌株细胞的生长与发酵是同步的,最适种龄为8~18h,产乳链菌肽表现出初级代谢动力学特征;
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  primary metabolite
4-Hydroxyifosfamide is the primary metabolite in vivo of the bifunctional alkylating cytostatic ifosfamide.
      
Simultaneous determination of difloxacin and its primary metabolite sarafloxacin in rabbit plasma
      
Simultaneous determination of enrofloxacin and its primary metabolite, ciprofloxacin, in chicken tissues
      
Primary metabolite measurements showed no nutritional differences between the two squash varieties.
      
Surprisingly, some genes show high similarity to primary metabolite genes not commonly identified in any antibiotic biosynthesis cluster.
      
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  primary metabolism
Erythromycin biosynthesis is a highly complicated process, which involves both primary metabolism and secondary metabolism.
      
Exogenously added amino acids were mostly utilized in primary metabolism.
      
Organic acids, such as lactic, malic and citric acids, are involved in primary metabolism of all living organisms.
      
It was found that the shear rate below 458 s-1 enhanced the primary metabolism, increasing mitochondrial activity and protein expression and inhibiting the activity of phenylalanine ammonia lyase (PAL), an enzyme relevant to the secondary metabolism.
      
The high 'degree of chemical freedom' of secondary metabolism which, in contrast to primary metabolism, allows structural modifications with almost no restrictions, is stressed as mechanistic basis for the generation of chemical diversity.
      
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The mechanism of short fatty acids on the biosynthesis of salinomy-cin was studied by using 14-C labelled compounds and radioimmunoassay methods. The results showed that acetate was of benefit to the primary metabolism of the strain and malonate was of benefit to the secondary metabolism of the strain and that 0.25 percent acetate could stimulate cell growth and increase the amount of cAMP(cylic s's'-adenosine monophosphate)but decreased the biosynthesis of salinomycin a great extent,On the contrary,0.25 percent...

The mechanism of short fatty acids on the biosynthesis of salinomy-cin was studied by using 14-C labelled compounds and radioimmunoassay methods. The results showed that acetate was of benefit to the primary metabolism of the strain and malonate was of benefit to the secondary metabolism of the strain and that 0.25 percent acetate could stimulate cell growth and increase the amount of cAMP(cylic s's'-adenosine monophosphate)but decreased the biosynthesis of salinomycin a great extent,On the contrary,0.25 percent malonate significantly stimulated the to biosynthesis of salinomycin but obviously decreased the amount of cAMP.

本文采用14-C标记化合物及放射免疫分析技术,研究了短链脂肪酸在盐霉素生物合成过程中的代谢途径及其对产生菌环-磷酸腺苷(cAMP)的影响和对盐霉素合成的影响.实验结果表明:乙酸有利于产生菌的初级代谢,丙二酸则有利于产生菌的次级代谢.0.25%浓度的乙酸能够促进菌体生长和提高胞内外cAMP浓度,但盐霉素合成受到明显抑制;0.25%浓度的丙二酸能够明显地促进盐霉素的合成,但其产生菌胞内cAMP浓度明显降低.

Spiramycin biosynthesis is liable to be subjected to carbon and nitrogen cataboltte repression and end product inhibition. Spiramycin fermentation is highly sensitive to its operation conditions, since there are numerous variables which affect the fermentation process. However, only a few of them can be monitored and controlled during the fermentation. Thus,the correlations between ATP and spiramyc-ia synthesis and other variables in the course of fermentation were studied. It was found that high level of ATP...

Spiramycin biosynthesis is liable to be subjected to carbon and nitrogen cataboltte repression and end product inhibition. Spiramycin fermentation is highly sensitive to its operation conditions, since there are numerous variables which affect the fermentation process. However, only a few of them can be monitored and controlled during the fermentation. Thus,the correlations between ATP and spiramyc-ia synthesis and other variables in the course of fermentation were studied. It was found that high level of ATP in the culture broth was unfavorable to spiramycin biosynthesis. Besides, compared with the control, ATP level was lower, glucose consume rate was less, while average spiramycin potency was enhanced by 42% in the fermentation batches with vegetable oil supplement. Dissolved phosporous compound cotent could affect the generation of ATP and thereby the biosynthesis of spiramycin. Nev ertheless, the supplement of KH2PO4 at late stage of the fermentation had a favorable effect on spiramycin production. It was elucidated in resting cell system that phosphate played the role of alleviating the glucose and NH4 repression and end product inhibition of spiramyctn biosynthesis, and promoting the biosynthesis and glycosidation of the sugar moieties precusors to macrolide.

螺旋霉素的生物合成受碳、氮分解代谢物的阻遏和终点产物的抑制。螺旋霉素发酵对工艺条件很敏感,影响发酵过程的因素很多,且错综复杂。对发酵控制可资借鉴的参数极少。为此,本文研究了发酵过程中ATP的变化与螺旋霉素生物合成的关系,从中发现,过程ATP水平过高,对SPM生物合成不利,因基质主要用于初级代谢。另外,基础配方加油的批号比不加油的批号的ATP水平低,糖耗小,SPM单位平均增长42%。ATP含量高会抑制脂肪的代谢。发酵液的溶磷会影响ATP的含量,从而影响发酵单位。但发酵后期加入少量磷酸盐有利于SPM的合成,静息细胞系统中证明磷酸盐的作用在于减轻葡萄糖和NH_4~+的阻遏和SPM的反馈抑制,还促进SPM中三个糖的合成与连接。

A mutant 880103 was obtained from parent strain A03 by an improved selection procedure of mutant. The selection of new mutant and the result of test production was described in this article.

麦迪霉素产生菌生米加链霉菌A03菌株经过多次诱变筛选及抗自身代谢物突变,得到了产量正变株;通过推理筛选的方法,应用初级代谢产物生物合成途径的调节突变株,分离得到了一主要组分麦迪霉素A_1高于对照菌株的优良菌株880103。并通过加入前体的方法,使其麦迪霉素A_1组分进一步提高。在试产中,该菌株与对照菌株相比,发酵单位相对提高16%,麦迪霉素 A_1组分相对提高 28%,成品效价相对提高4.7%;且发酵周期短、温度适中、遗传性能稳定、适应性强,是一株优于对照菌株的优质高产菌株。

 
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