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     accepting this premise, the presence of dsRNA in a plant is a footprint of an RNA virus or Virus-like agents. The dsRNA detected could be the genome of a dsRNA virus or the replicative dsRNA of a single-stranded RNA (ssRNA) virus.
     在植物体内dsRNA是RNA病毒和类病毒因子存在的迹象,dsRNA包括dsRNA病毒的基因或ssRNA病的复制中间型
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     BEAUTIFUL COPY
     完美复制
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     THE REPLICATION OF CHROMOSOME
     染色体的复制
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     3)intermidiate tpye;
     3.中间型
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     (3)semidwarf with medium long leaf andmedium dense panicle;
     3型是中间型;
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     accepting this premise, the presence of dsRNA in a plant is a footprint of an RNA virus or Virus-like agents. The dsRNA detected could be the genome of a dsRNA virus or the replicative dsRNA of a single-stranded RNA (ssRNA) virus.
     在植物体内dsRNA是RNA病毒和类病毒因子存在的迹象,dsRNA包括dsRNA病毒的基因或ssRNA病的复制中间型
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  replicative intermediate
The replicative form and the replicative intermediate of TMV-RNA were isolated from synchronously infected tobacco leaves, labeled with H3-uridine for 1 hour.
      
The replicative intermediate showed only partial resistance to RNase and heterogeneous sedimentation behavior in sucrose gradients.
      
After mild RNase treatment the replicative intermediate sedimented homogeneously, and with an S value slightly lower than the replicative form.
      
Electron microscope examination and measurements of the EcoRI treated replicative intermediate molecules indicate that replication can be initiated at two sites on the plasmid DNA molecule.
      
Therefore, in some sequences integrated by subgenomic HBV DNA, the integration of replicative intermediate of the HBV minus strand DNA into the chromosomal DNA may take place and one of the viral integration sites seemsto be in the region X.
      
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The principle and techniqes of dsRNA analysis was summarized for the identification of RNA viruses. The dsRNA approach is based on the premise that plants not infected with RNA viruses or virus-like agents do not Contain readily detectable amount of homogeneous Segements of high molecular Weight (>0.1×106) dsRNA. accepting this premise, the presence of dsRNA in a plant is a footprint of an RNA virus or Virus-like agents. The dsRNA detected could be the genome of a dsRNA virus or the replicative dsRNA of a single-stranded...

The principle and techniqes of dsRNA analysis was summarized for the identification of RNA viruses. The dsRNA approach is based on the premise that plants not infected with RNA viruses or virus-like agents do not Contain readily detectable amount of homogeneous Segements of high molecular Weight (>0.1×106) dsRNA. accepting this premise, the presence of dsRNA in a plant is a footprint of an RNA virus or Virus-like agents. The dsRNA detected could be the genome of a dsRNA virus or the replicative dsRNA of a single-stranded RNA (ssRNA) virus. the dsRNA quite stable and can usually be isolated by physical or chemical method. Techniques for the isolation, purification and analysis of dsRNA were described.Applications of dsRNA analysis to the defection of plant viruses,the 19 groups of plant viruses、 cryptic virus、 viruses of a crop, the 26 solates of CMV, Satellites RNA. virold, etc. were identified by dsRNA analysis.

dsRNA技术是以无RNA病毒或类似病毒因子侵染的植物组织中不含有容易鉴定的同原大分子的dsRNA(>0.1×106)存在为前提。在植物体内dsRNA是RNA病毒和类病毒因子存在的迹象,dsRNA包括dsRNA病毒的基因或ssRNA病的复制中间型。dsRNA在寄主组织相当稳定,可以用物理和化学的方法分离出来。叙述了dsRNA技术用于植物病毒群、成员、同一病毒的不同株系、感染作物的新病毒及其复合侵染、类病毒、卫星病毒等的鉴定。证明该方法使用广泛、快速准确,不需要贵重设备,在一般实验室中即可进行。该方法有重要应用和学术价值,已引起植物病毒和植物病理学工作者广泛兴趣。

In vivo transfection with a plasmid-linked tandem DHBV DNA dimer on 2-day old DHBV-free Furong ducklings was carried out. Most of the animals (86%) developed transient viremia. Serum DHBs/pre S Ag and DHBV DNA appeared on 9th day, reached to its peak on12th~14th day and disappeared on 28th after transfection. Whereas, there were a few ducks whose viremia persisted for more than 50 days. DHBV DNA replicative intermediates were alsofound in the liver tissue of transfected ducks. DHBV intact viral panicals were...

In vivo transfection with a plasmid-linked tandem DHBV DNA dimer on 2-day old DHBV-free Furong ducklings was carried out. Most of the animals (86%) developed transient viremia. Serum DHBs/pre S Ag and DHBV DNA appeared on 9th day, reached to its peak on12th~14th day and disappeared on 28th after transfection. Whereas, there were a few ducks whose viremia persisted for more than 50 days. DHBV DNA replicative intermediates were alsofound in the liver tissue of transfected ducks. DHBV intact viral panicals were detected in viremic sera under electronic microscope. Intraperitoneal injection of the transfected ducks' viremic sera into 1-day old DHBV-free ducklings resulted in productive DHBV infection on 60% animals,demonstrating the production of biologically active virus after in via transfection. The establishmant of this method is valuable for the research of DHBV variant and the relation between DHBVgene structure and its function.

用一种含头尾相连DHBVDNA双体的质粒体内转染2日龄芙蓉鸭,大多数鸭(86%)产生了短暂病毒血症。血清DHBs/preSAg和DHBVDNA于转染后第9天出现,第12~14天达峰值,第28天时多数转阴;少数鸭的病毒血症可持续50天以上。转染鸭肝组织中也检测到复制中间型DHBVDNA的存在。用转染鸭病毒血症期的血清作磷钨酸负染电镜观察,找到了完整的DHBV病毒颗粒,并且用此血清腹腔注射1日龄鸭,60%的鸭被感染成功,证明体内转染后有生物活性的DHBV病毒颗粒的产生。该研究方法的建立.对于研究DHBV变异株.DHBV基因结构与功能的关系等,均有一定理论意义及应用价值。

 
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