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   体细胞转变 的翻译结果: 查询用时:0.419秒
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体细胞转变
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     After that time, the somatic embryos swelled and bacame brown, finaly lost their viability.
     超过12天后,体细胞胚由绿色向褐色转变,体细胞胚肥大肿胀,最后死亡。
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  somatic conversion
This variegation is caused by somatic conversion (paramutation).
      
Within the subgenus Eulycopersicon the frequency of somatic conversion (paramutation) is - within the range of random and modificative fluctuations - determined only by the conversion activity (paramutagenicity) of the special sulf allele present.
      
pennellii, however, intensely inhibit the occurrence of somatic conversion in F1.
      
New cases of somatic conversion (paramutation) in tomato (Lycopersicon esculentum Mill.)
      
In this organism only one VH gene is usually expressed, and other VH genes or pseudogenes are used for somatic conversion of this expressible gene.
      
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It was an important turning point of cell differentiation to transform somatic cell into embryonic cell during somatic embryogenesis of plant. It was,therefore,very important to study cell differentiation of embryonic cell. In view of this,the subject of this study is Lycium barbarum L.during its early stage of embryonic cell,the differentiation and development of it is described the ultrastructural characteristic. The results indicated (1) Embryonic cell of Lycium barbarum L.mostly come from the parenchyma...

It was an important turning point of cell differentiation to transform somatic cell into embryonic cell during somatic embryogenesis of plant. It was,therefore,very important to study cell differentiation of embryonic cell. In view of this,the subject of this study is Lycium barbarum L.during its early stage of embryonic cell,the differentiation and development of it is described the ultrastructural characteristic. The results indicated (1) Embryonic cell of Lycium barbarum L.mostly come from the parenchyma cells of callus epiderm. In callus undifferentiation cells were bulky,irregular,thin walls,a small nucleus,a few cytoplasm and so on.(2) Embryonic cells were egg in shape,had a large nucleus,dense cytoplasm and rich organelle content.(3) Late embryonic cells which thickened cell wall,disappeared plasmodesmate,deviated nucleus and formed large vacuole. (4) The first division of embryonic cells in which bicellular stage were formed was mostly equal. When bicellular stage continuously broke up into multi\|cellular stage,the cells of embryo had a large nucleus,the nucleus were irregular in shape,dense cytoplasm,rich organelle and starch accumulation. 

在植物体细胞胚发生过程中,体细胞转变为胚性细胞是细胞分化的转折点,因此对胚性细胞分化的研究是十分重要的。为此,本研究以枸杞胚性细胞的分化和发育早期为重点,用半薄切片定位,进行超薄切片,以观察胚性细胞超微结构的特点。结果表明:(1)枸杞的胚性细胞多由愈伤组织表层的薄壁细胞分化而来;愈伤组织中未分化的细胞体积大,细胞壁薄,形状不规则,一般都具有中央大液泡,核小,细胞质少,细胞器少。(2)胚性细胞呈卵圆形,细胞核大,核仁明显,细胞质浓厚并含有丰富的细胞器。(3)胚性细胞发育到晚期,细胞壁加厚,胞间连丝逐渐消失,细胞核向一端偏移,有大液泡形成。(4)胚性细胞的第一次分裂多为均等分裂,形成二细胞原胚,继续分裂,发育形成多细胞原胚。组成多细胞原胚胚体的细胞核大,核形状不规则,细胞质浓厚,细胞器丰富,在质体中出现淀粉积累。

When calluses of Lycium barbarum L. were transferred into differentiation medium the putrescine content rapidly increased and reached to the first peak,then droped relatively .The putrescine content increased again and arrived at the highest peak (six times of control) in multicellular preembryo stage.Spermidine was only synthesized in early stag of somatic embryo development.Spermine was discovered only in later stage of somatic embryo development.Exogenous Put not only raised the rate of somatic embryogenesis...

When calluses of Lycium barbarum L. were transferred into differentiation medium the putrescine content rapidly increased and reached to the first peak,then droped relatively .The putrescine content increased again and arrived at the highest peak (six times of control) in multicellular preembryo stage.Spermidine was only synthesized in early stag of somatic embryo development.Spermine was discovered only in later stage of somatic embryo development.Exogenous Put not only raised the rate of somatic embryogenesis but also increased content of three kind endogenous polyamine abovementioned.Supplement of DFMA which was an inhibitor of polyamine biosynthesis could make the content of polyamine drop and somatic embryogenesis block.Polyamine biosynthesis was renewed partly and DFMA inhibition was removed partly when exogenous Put was supplemented.The result showed that polyamine level was essential somatic embryogenesis.

完全脱分化的枸杞继代愈伤组织在转入分化培养后第 1天就开始启动分化 ,接着体细胞转变为胚性细胞 ,继而分裂形成多细胞原胚、球形胚和成熟胚等。与此同时 ,Put含量迅速上升形成第 1个峰值 ,随后有所下降 ,但到多细胞原胚期 Put含量又上升 ,并达到最高峰 ,为对照的 6倍。 Spd只在胚性细胞分化早期出现 ,Spm仅在体细胞胚发育晚期存在。外源 Put不仅可提高体细胞胚发生频率 ,而且使 3种内源多胺含量均有所提高。加入多胺生物合成抑制剂 DFMA后 ,多胺水平下降 ,体细胞胚发生几乎完全被抑制。补充外源 Put后 ,多胺的生物合成得到部分恢复 ,DFMA对体细胞胚发生的抑制效应也被部分解除。结果表明 ,维持一定量的多胺水平是枸杞体细胞胚发生的必要因素

 
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