Objective : To reveal the mechanism of action that fucoidan counteracts renal interstitium fibrosis,we investigated the effects of fucoidan(FPS) on proliferation , apoptosis and expression of fibronectin(FN),laminin(LN) and transforming growth factorβ1(TGF-β1) in the human renal interstitial fibroblast(HRIF) in vitro.
The results showed that expression of Fas/APO1 was strong in T24 cell line and in primary in vitro cultured renal fibroblast, medium in PC3M cell line, weak in RCC949 and BIU87 cell lines and negative in GRC1 and EJ cell lines.
To explore the role of Fas/APO1 in the occurrence and progression of human urinary malignant tumors, expression of Fas/APO1 was tested with immunocytochemical technique in 6 kinds of urinary tumor cell lines consisting of bladder cancer (T24, EJ, BIU87), renal cell carcinoma (GRC1,RCC949),prostatic cancer (PC3M) and a primary in vitro cultured normal renal fibroblast.
Conclusion Possibly through abrogating TGF-β1/Smads signaling pathway, PPARγ agonists inhibit TGF-β1-induced renal fibroblast extracellular matrix synthesis and may play a potential role in preventing tubulointerstitial fibrosis as a novel approach to prevent the progress of end stage renal dysfunction.
Transmission electron microscopy of autopsy specimens from the SCHADD patient demonstrated that most renal interstitial fibroblasts and ～50% of fibroblasts in the heart had microvesicular steatosis.
Prohibitin suppresses renal interstitial fibroblasts proliferation and phenotypic change induced by transforming growth factor-β
Therefore, over-expression of PHB suppresses renal interstitial fibroblasts proliferation and cell phenotypic change induced by TGF-β1, which indicates PHB as a potential therapeutic target to halt the progression of TIF.
Erk-1/2 activation is sufficient for MMP-9 induction in renal interstitial fibroblasts.
HGF does not increase the protein levels of Smad transcriptional co-repressors in renal interstitial fibroblasts.
The effects of cardiac myocytes on interstitial fibroblasts in toxic iron overload
There is increasing evidence that lipid interstitial fibroblasts play an important role in injury-repair mechanisms in various organ systems.
We detected lacZ expression exclusively in the interstitial fibroblasts near the PTCs of the kidney by immunoelectron microscopy.
Vimentin-positive cells are identified as interstitial fibroblasts, capillary endothelia and other vascular wall cells as well as practically all glomerular cells, including podocytes and mesangial cells.
MMP-2 was mildly expressed in the interstitial fibroblasts and MMP-3 was faintly stained in the extracellular matrix of the synovial membrane.