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酶谱技术
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  “酶谱技术”译为未确定词的双语例句
     Methods The expressions of MMP-2 and MMP-9 over a time course ranging from 3 h to 7 d after reperfusion were measured by gelatin-containing SDS-PAGE Zymography.
     方法 应用含明胶的定量酶谱技术,检测大鼠局灶性脑缺血再灌注7 d内MMP-2和MMP-9的表达。
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     The expression of MMP-2 and MMP-9 in rat brains over a time course ranging from 3h to 7d after reperfusion was measured by gelatin-containing SDS-PAGE zymography.
     应用含明胶的定量酶谱技术检测再灌注3h到7d内MMP-2、MMP-9的表达,用甲酰胺浸泡法检测大鼠脑内EB含量。
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     The authors have analysed the changes of peroxidase isozyme of 18 parents in 13 hybrid combinations in the sprouting stage of tomato by m-eans of the zymogramic technique.
     作者用酶谱技术分析了番茄13个组合的18个亲本芽期过氧化物酶同工酶变化情况。 按生长顺序分三次取样。
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  相似匹配句对
     Technology;
     技术
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     TECHNOLOGY
     技术
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     The results also show that the relations of E.
     从POD酶谱上分析,E.
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     QUANTITATIVE ANALYSIS OF ESTERASE ISOZYMES IN RICE
     水稻酯酶酶谱的定量分析
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     Myocardial enzymes were measured.
     监测心肌酶谱变化。
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  zymogram technique
A newly developed zymogram technique was positive for amylase activity in all 10 protein bands.
      
In order to detect the plasminogen phenotypes the immunofixation technique was more suitable than the zymogram technique.
      
Genetic polymorphism of the glucose dehydrogenase in white cells extracts from random adult Japanese was investigated using polyacrylamide gel isoelectric focusing or agarose gel isoelectric focusing, followed by a specific zymogram technique.
      
Genetic variability in 6 neotropical anopheline species, analyzed, by zymogram technique, has been described.
      
Four major xylanases were detected in the cell-associated fraction using the zymogram technique.
      
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In this article are reported preliminary experimental results of the changes in isozymes of peroxidase,esterase and cytochrome oxidase in rice sprouts irradiated by ~(60)Co γ-rays.

本试验用聚丙烯酰胺盘状凝胶电永法研究了在~(60)Coγ—射线作用下,水稻芽中酯酶等同工酶的变化情况。通过酶谱技术显示的谱带图样看出:与对照比较,处理的水稻芽中酯酶同工酶,缺少酶带3,增多酶带8′,各酶带活力降低,处理的水稻芽中过氧化物酶同工酶,缺少酶带4,增多酶带4′,各主要酶带活力增高,细胞色素氧化酶同工酶处理与对照相同。结果表明,水稻芽中酯酶,过氧化物酶同工酶的变化与~(60)C0γ—射线作用有密切关系,而此二酶可衡量遗传效应。因此,分析同工酶的变化规律,有可能为预测辐射的遗传效应,探讨辐射的作用机理提供依据。

The authors have analysed the changes of peroxidase isozyme of 18 parents in 13 hybrid combinations in the sprouting stage of tomato by m-eans of the zymogramic technique. The samples were taken at 3 times during plant development. The zymogram patterns shown may be classifi-ed into two types and four subtypes:

作者用酶谱技术分析了番茄13个组合的18个亲本芽期过氧化物酶同工酶变化情况。按生长顺序分三次取样。酶谱表现可划分为两种类型、四种亚型,即: 第一型(F型)表现为第一次取样谱带数多,酶活性较强。F—a亚型表现为从第一次取样到第二次取样酶谱带数增加,酶活性也增强;但第三次取样的酶谱带数则比第二次取样减少,酶活性也减弱。F一b亚型表现为从第一次取样到第三次取样谱带数一直增加,酶活性也持续较强。 第二型(S型)表现为第一次取样谱带数少,酶活性较弱。S—a亚型表现为从第一次取样到第三次取样谱带数逐渐增多,酶活性增强。S—b亚型表现为从第一次取样到第三次取样谱带数变化较小,酶活性也一直较弱。 实验探讨了亲本酶谱型和杂种优势之间的关系。高优组合的父本多为F型,母本多为S型。两亲本酶谱差异适宜,把两个亲本的第三次取样的酶谱扫描曲线绘在同一座标中呈多点交叉。低优组合则无此交叉。这些,可为番茄杂种优势利用的亲本选配、早期预测等工作开辟一条新途径。

A Chinese boy with Hb Q-H disease was studied by restriction endonuclease mapping to analyse the organization of the α-globin genes. DNA was isolated from peripheral blood leukocytes and from spleen tissues of the patient, and then digested with restriction endonucleases Eco EⅠ and Bgl Ⅱ, respectively. The digested DNA was transferred to a nitrocellulose filter after agarose gel electrophoresis. DNA fragments on the nitrocellulose filter was hybrized with (32P) α-cDNA and autoradiographed.When 32P-α-cDNA was...

A Chinese boy with Hb Q-H disease was studied by restriction endonuclease mapping to analyse the organization of the α-globin genes. DNA was isolated from peripheral blood leukocytes and from spleen tissues of the patient, and then digested with restriction endonucleases Eco EⅠ and Bgl Ⅱ, respectively. The digested DNA was transferred to a nitrocellulose filter after agarose gel electrophoresis. DNA fragments on the nitrocellulose filter was hybrized with (32P) α-cDNA and autoradiographed.When 32P-α-cDNA was used as probe, the normal 23 kb Eco RI fragment and the 12.5 kb Bgl Ⅱ fragment were absent but the 19 kb Eco RI fragment and 7.7 kb Bgl Ⅱ fragment were present in the endonuclease digestion patterns. This thus indicated that this case of Hb Q-H disease corresponds to a leftward type (4.2 kb) deletional form of α-thalassemia and the genotype of patient is -α0/--. That is, both α-globin genes (α1 and α1) deleted in one No. 16 chromosome and the α2 gene deleted in another No. 16 chromosome bearing a mutational a-globin gene (α0) on the α1 locus.

本文报道用限制性内切酶酶谱技术对我国江西一例HbQ-H病人进行α-珠蛋白基因分析,研究结果表明,本例HbQ-H病属左侧缺失型α-地中海贫血,患者的基因型为-α~Q/--。

 
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