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解链
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  melting
     METHODS Melting curves were determined in the DNA microarray,in terms of the thermodynamic traits of DNA duplexes,and the Tm values was calculated for SNP analysis.
     方法根据DNA二聚体的热力学性质,在DNA阵列芯片上测定其解链曲线,用数学的方法获得其解链温度(Tm)值,再根据Tm值进行SNP分析。
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     The G+C content of the DNAs ranged between 42.9 and 72.1 mol% as estimated from their melting temperatures.
     根据解链温度计算出噬菌体DNA的G+C含量在42.9与72.1mol%之间。
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     Predicting Melting Temperature of DNA Duplex by BP Neural Network
     基于BP神经网络的DNA解链温度预测模型
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     Its melting temperature is 80℃.
     解链温度为80.0℃。
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     From the UV melting temperature measurements, we find that TETA is able to stabilize the G-quadruplex structures with the △Tm of about 10℃ and 26℃ for d(TTAGGG)4 and d(TGGGGT) respectively at the TETA concentration of 100 μM.
     对TETA与寡核苷酸作用后的DNA热变性曲线的分析发现,当TETA的浓度为100μM时,与K~+存在条件下相比,寡核苷酸d(TTAGGG)_4和d(TGGGGT)形成的G4-DNA的解链温度分别升高了10℃和26℃,表明TETA能显著增强G4-DNA的稳定性。
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  unwinding
     The DNA helioaae activity of BstH1 requires a 3'-terminal single-stranded DNA binding site to initiate the unwinding reaction in the 3'→5' direction.
     BstH1具有DNA解链活力,解链反应需要3'端单链DNA结合区并以3'→5'方向进行解链
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     DNA unwinding assay′s principle and application
     DNA解链分析法的原理及应用
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     DNA Unwinding and Control of Information Expressions
     DNA解链和信息表达的调节
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     These changes include the unwinding, breaking and crosslinking of DNA double chain, the inserting of intercalators, the dropping bases, the replacing of abnormal bases, the chemical modifying of bases and the changing of relative high-order structure of DNA.
     原则上,DNA分子结构的任何异常变化均称为DNA损伤,包括DNA双螺旋的非正常解链、链的断裂与交联、嵌合剂在双链间的插入、碱基的异构化与脱落、非正常碱基的掺入、碱基上的化学修饰以及由此引起的DNA二级结构、三级结构的变化等等。
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     HCV helicase is responsible for unwinding of RNA genome and play a key role in the replication of HCV genome. The HCV helicase gene was amplified by RT-PCR from the HCV positive serum.
     解旋酶(helicase)在HCV基因组复制中负责RNA的解链,在复制中起着关键的作用。
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  “解链”译为未确定词的双语例句
     The CPV-dsRNA were denatured and melted into ssRNA with 90% dimethyl sulfoxide dr 8M urea.
     我们采用90%的二甲基亚砜或8M的尿素使CPV—dsRNA解链.
短句来源
     Purification and Characterization of DNA Helicase BstH1 from Bacillus stearothermophilus
     嗜热脂肪芽孢杆菌DNA解链蛋白1的纯化及性质
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     The poly- (2-(dimethylamino) ethyl methacrylate)-b- potassium polyacrylate (PDMAEMA-b-PPA) with pH and temperature response was polymerized by Reversible Addition-fragmentation Transfer. The Mn and Mw/Mn of PDMAEMA-b-PPA were 24500g/mol and 1.10, respectively.
     通过可逆加成-裂解链转移法(Reversible Addition-fragmentation Transfer,RAFT)制备嵌段聚合物PDMAEMA-b-PPA,分子量为24500 g/mol,分布为1.10。
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     We have partially Purified a DNA helicase BstH1 from Bacillus stearothermophilus through Polymin P precipitation,ammonia sulfate precipitation and column chromategraphic steps with Phenyl-Sepharose,DEAE-cellulose, phosphocellulose,FPLC Mono Q and Superose 12.BstH1 possesses a DNA-dependent ATPase activity in the presence of Mg2+.
     以嗜热脂肪芽孢杆菌(Bacillusstearothermophilus)为材料,通过PolyminP沉淀、硫酸铵分级及Phenyl-Sepharose、DEAE纤维素、磷酸纤维素、FPLCMonoQ、FPLCSuperose12等柱层析,得到了部分纯化的DNA解链蛋白1(BstH1)。
短句来源
     MALDI-TOF-MS showed that the highly expressed proteins were: an unknown protein,proliferation-associated gene A,Up1,alternative splicing factor ASF-3,cofilin1(non-muscle),eukaryotic translation initiation factor 5A,beta galactoside binding lectin,and glutathione-S-transferase Pi.
     质谱分析高丰度表达的差异蛋白分别为:未知蛋白、增殖相关基因A、解链蛋白Up1、交替拼接因子ASF-3、cofilin 1、真核转录启动因子5A、β半乳糖苷酶结合凝集素、Pi类谷光苷肽-S-转移酶。
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  melting
Ultraviolet (UV) melting curves of the DNA at 260 nm as well as the calorimetric measurements were used to estimate the binding constants (K), melting enthalpy (ΔH°m) and binding enthalpy (ΔH°b).
      
Both infrared spectra and melting point of the compound were consistent with those reported earlier.
      
It was found by DSC measurements that the melting point of samples gradually shifted to lower temperatures with the decrease of the solution concentration used for sample preparation.
      
The yield of the final product was 78%, and the melting point was 231-233°C.
      
The thermotropic properties, the melting point (Tm) and the isotropization temperature (Ti) of the synthesized polyurethanes were characterized by DSC, IR and POM.
      
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  unwinding
At the same time, in comparison with the spiral unwinding flights from low near-circular orbits, this scheme minimizes the harmful effect of the radiation belts.
      
A joint application of four optimizing transformations for purging imperative programs-elimination of useless statements, unwinding of degenerate loops, removal from loops, and removal from branch statements-is considered.
      
Algorithms for the elimination of useless statements, unwinding of degenerate loops, and removal from loops and hammocks are described.
      
Calculations for the unwinding (winding) of the helix in the process of the jump were performed to investigate the case of infinitely strong anchoring on one surface and finite anchoring on the other, which is important in applications.
      
These solutions describe the so-called spherulitic domains which have been observed in a large-pitch cholesteric near the unwinding transition.
      
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Surfactants in low concentration were used to study their effects on K~+ transport of excised roots.The surfactants used are:cetyltrimethylammonium chloride (CTMA, cationic),sodium dodecyl sulfate (SDS, anionic) and polyoxyethylene sorbitan monolaurate (Tween-20, non-iomc).

表面活性剂对小麦离体根K~+吸收和呼吸的抑制效应不同于解链剂DNP,表现在表面活性剂对小麦离体根K~+吸收的抑制作用大于它对呼吸的抑制作用,和对KCI刺激的呼吸几乎没有影响;而DNP对小麦离体根呼吸的影响大于它对K~+吸收的抑制作用,并几乎消除了KCI刺激的呼吸。看来两者通过不同的途径影响小麦离体根K~+的吸收,DNP的抑制作用主要是氧化磷酸化解链的次生效应,而表面活性剂则可能直接对细胞膜和细胞膜的K~+传递系统起作用。三种不同类型的表面活性剂对小麦离体根K~+吸收显现出某些不同的效应,阳离子型表面活性剂氯化十六烷基三甲基铵(CTMA)和非离子型表面活性剂聚氧乙烯山梨醇月桂酸酯(Tween-20)在低浓度时对小麦离体根K~+吸收有促进作用,阴离子型表面活性剂十二烷基硫酸钠(SDS)没有这种表现。这种现象可能与表面活性剂带电荷的性质,和它们与膜的作用方式有关。对CTMA和SDS引起小麦离体根K~+外流的溶液紫外吸收光谱检测的结果表明,有在260和280mμ呈现吸收峰的物质从根上掉下来,并与Folin酚试剂起呈色反应,这些物质可能与小麦离体根K~+吸收能力的丧失有联系。

Three colony types (330 S, 330 L, and 330 V) were isolated from a cowpea Rhizobium sp. strain 330. Some of their characteristics were studied. Both symbiotic and free-living (non-associative) nitrogen fixation analysis revealed that the strain 330Ⅴ was significantly more effective than the 330 S, that the strain 330 S was moderately effective, whereas that the strain 330 L was ineffective. On the basis of comparing their properties, it was found that five characteristics seem to be correlative: High non-associative...

Three colony types (330 S, 330 L, and 330 V) were isolated from a cowpea Rhizobium sp. strain 330. Some of their characteristics were studied. Both symbiotic and free-living (non-associative) nitrogen fixation analysis revealed that the strain 330Ⅴ was significantly more effective than the 330 S, that the strain 330 S was moderately effective, whereas that the strain 330 L was ineffective. On the basis of comparing their properties, it was found that five characteristics seem to be correlative: High non-associative nitrogenase activity, high symbiotic nitrogen-fixing activity formed by a strain with cowpea host plant, small colony morphology, long doubling time, and low specific viscosity.The three colony types were all gram-negative rods (0.70×2.0 μ) with polar flagella. The thermal denaturation temperature of their DNAs was same. It corresponds to a mole per cent quanine plus cytosine composition of 60%. The soluble antigens from these strains and antibodies from anti-330 S serum separately form a precipitin band in gel-immune diffusion. Experiment showed that 330Ⅴ and 330 S were antigenically identical and that 330 L was related, but not identical, to 330Ⅴ or 330 S. Their sensitivity to 50 mM NaCl was similar. Their correlation between relatives was discussed in the paper.

从豇豆根瘤菌330菌株分离获得三株菌落型(330S,330L和330V)。共生固氮和自生(非共生)固氮研究表明,330V的固氮作用比330S明显有效,330S中等有效,而330L无效。对它们的一些特征作了比较,看来有五个特征是有相关性的:非共生固氮活性高,菌株与豇豆宿主形成的共生固氮活性高,菌落形态小;世代时间长;比粘度低。 这三个菌落型均为革兰氏阴性杆菌,菌体大小为0.7×2.0微米,都有极生鞭毛。它们DNA的解链温度相同。在凝胶免疫扩散中,各菌株的可溶性抗原分别与330S抗血清的抗体形成一条沉淀带,330S与330V抗原性相同,330L与330S或330V有关但不同一。它们对50毫克分子盐的敏感度亦相似。本文讨论了它们的亲缘关系。

Vanadate inhibited K~+ uptake and H~+ extrusion in wheat roots.The inhibitory effect of vanadate on K~+ uptake and H~+ extrusion was not parallel.Depression of H~+ extrusion by vanadate began at 5 minutes after commencement of experiment and the inhibition of K~+ uptake occurred at 15 minutes.In the presence of vana-date,K~+ uptake in low rate continued,but H~+ extrusion stopped after 30 minutes. Vanadate inhibited plasma mem-brane ATPase of wheat roots in vitro.The mode of inhibition of vanadate is non-competitive.While...

Vanadate inhibited K~+ uptake and H~+ extrusion in wheat roots.The inhibitory effect of vanadate on K~+ uptake and H~+ extrusion was not parallel.Depression of H~+ extrusion by vanadate began at 5 minutes after commencement of experiment and the inhibition of K~+ uptake occurred at 15 minutes.In the presence of vana-date,K~+ uptake in low rate continued,but H~+ extrusion stopped after 30 minutes. Vanadate inhibited plasma mem-brane ATPase of wheat roots in vitro.The mode of inhibition of vanadate is non-competitive.While excised wheat roots being immersed in KCLsolution,vanadate enhanced ATP level in root cells.It is suggested that plasma membrane ATPase may being inhibited by vanadate in vivo and so depressing the dephosphoryla-tion of ATP. K~+ uptake was inhibited imme-diately after treatment with azide,but the H~+ extrusion was not inhibited until 15 minutes after experiment.No inhibitory effect of azide on plasma membrane ATPase activity in vitro was observed. From above results,mode of H~+/K~+ exchange and plasma membraneATPase action are discussed.

Neurospora细胞膜质子泵(H~+-ATPase)专一性抑制剂钒酸钠,抑制小麦离体根K~+的吸收与H~+分泌,并抑制小麦根细胞膜-K~+-Mg~(2+)-ATPase活力。它对K~+吸收的抑制效应,可能是抑制质膜K~+-Mg~(2+)-ATPase活力的结果。而且在起抑制作用的时间上有明显地不同,表明钒酸钠对K~+、H~+在细胞膜中的通道影响不同。叠氮钠解链小麦根的呼吸,降低根细胞的ATP水平,但从实验开始就完全抑制小麦根K~+的吸收,对质膜K~+-Mg~(2+)-ATP-ase的活力没有影响。可能叠氮钠只阻止“载体”对K~+接受的过程。应用~86R_b+示踪的K~+吸收试验表明,钒酸钠对小麦根K~+吸收的抑制%,不为增加外部溶液K~+浓度而减低。增加底物ATP浓度,也不能减低钒酸钠对质膜-ATPase的抑制%。钒酸钠的抑制作用是非竞争性抑制。~3H-亮氨酸渗入试验表明钒酸钠对“载体”的合成没有干扰作用。VO_4~(3-)离子明显促进小麦根的呼吸,并提高根细胞的ATP水平,这种ATP水平的提高,可能是质膜-ATPase受到抑制,主动运输过程减弱的结果。

 
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