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野生蜂
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  “野生蜂”译为未确定词的双语例句
     Investigation on the Resources of Bombus in Jilin Province
     吉林省熊蜂野生蜂种资源调查
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     STUDY ON TRAPPING METHODS OF WILD MASON BEES ( OSMIA ))
     野生的采集技术研究
     Investigation on the Resources of Bombus in Jilin Province
     吉林省熊野生种资源调查
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     The original E(w(?)
     野生型E(W~(?)
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     tomentella and G.
     野生种G.
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     Killer Bees
     “杀人”无人机
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Task specialization in a wild bee, Apis florea (Hymenoptera: Apidae), revealed by RFLP banding
      
Flower-feeding specialization in wild bee and wasp communities in seasonal neotropical habitats
      
A total of 865 wild bee individuals was observed in the field, and 475 individuals were caught for species identification.
      
Blueberry and cranberry weight were related to bumble bee abundance but not to honey or other wild bee abundance.
      
Modem agriculture can negatively impact wild bee populations, resulting in lower yields in bee-pollinated crops.
      
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Random Amplified Polymorphic DNA(RAPD) is used to analyse the genetic diversity of the wild grouper (Epinephelus merra Bloch) population in Zhanjiang coastal waters. 16 Out of 120 primers are selected to carry out the analysis of RAPD and 154 loci are detected among 10 individuals. The percentage of polymorphic loci(P) is 65.58%. The average genetic similarity index(S) and the average genetic distance(D) are 0.788 7 and 0.211 3 respectively. The genetic diversity index(H) is 0.177 6. The result indicates that...

Random Amplified Polymorphic DNA(RAPD) is used to analyse the genetic diversity of the wild grouper (Epinephelus merra Bloch) population in Zhanjiang coastal waters. 16 Out of 120 primers are selected to carry out the analysis of RAPD and 154 loci are detected among 10 individuals. The percentage of polymorphic loci(P) is 65.58%. The average genetic similarity index(S) and the average genetic distance(D) are 0.788 7 and 0.211 3 respectively. The genetic diversity index(H) is 0.177 6. The result indicates that the genetic polymorphism of the wild fish(Epinephelus merra Bloch) is higher.

采用RAPD技术分析了湛江沿海野生蜂巢石斑鱼的遗传多样性 ,从 6个系列 1 2 0个引物筛选出1 6个引物 ,对 1 0尾蜂巢石斑鱼进行RAPD分析 ,1 6个引物共检测到 1 5 4个位点 ,其中 ,多态位点比例 (P)为 6 5 5 8% ,平均个体间遗传相似系数 (S)为 0 7887,平均个体间遗传距离 (D)为 0 2 1 1 3,遗传多样性指数 (H)为 0 1 776。研究结果表明 ,湛江沿海的野生蜂巢石斑鱼的遗传多样性较高。

Genotypes of four wild grouper species (Epinephelus merra Bloch, E. fario, E. malabaricus and E. fasciatus) in Zhanjiang littoral are analysed using 9 random primers by the method of random amplified polymorphic DNA (RAPD). The results sugguest that: (1) The inter- species genetic distance(Dij) are 0.4009(E .merra and E. fario), 0.4805 (E. merra and E. malabaricus), 0.5703 (E. merra and E. fasciatus), 0.4409 (E. fario and E. malabaricus), 0.4561(E. fario and E. fasciatus) and 0.4729 (E. malabaricus and E. fasciatus)....

Genotypes of four wild grouper species (Epinephelus merra Bloch, E. fario, E. malabaricus and E. fasciatus) in Zhanjiang littoral are analysed using 9 random primers by the method of random amplified polymorphic DNA (RAPD). The results sugguest that: (1) The inter- species genetic distance(Dij) are 0.4009(E .merra and E. fario), 0.4805 (E. merra and E. malabaricus), 0.5703 (E. merra and E. fasciatus), 0.4409 (E. fario and E. malabaricus), 0.4561(E. fario and E. fasciatus) and 0.4729 (E. malabaricus and E. fasciatus). (2) According to UPGMA, first, E. merra and E. fario are clustered together (0.4009); second, they and E. malabaricus are clustered (0.4611), finaly, all of them are clustered (0.4951). Therefore, the relationship of E. merra and E. fario is closer.

采用9个引物,对湛江沿海野生蜂巢石斑鱼、鲑点石斑鱼、点带石斑鱼及黑边石斑鱼的基因组DNA进行了RAPD分析,研究结果表明:蜂巢石斑鱼、鲑点石斑鱼、点带石斑鱼及黑边石斑鱼种间遗传距离(Dij)分别为0 4009(蜂巢石斑鱼-鲑点石斑鱼)、0 4805(蜂巢石斑鱼-点带石斑鱼)、0 5703(蜂巢石斑鱼-黑边石斑鱼)、0 4409(鲑点石斑鱼-点带石斑鱼)、0 4561(鲑点石斑鱼-黑边石斑鱼)及0 4729(点带石斑鱼-黑边石斑鱼);用UPGMA法对这4种石斑鱼进行聚类分析可知,蜂巢石斑鱼与鲑点石斑鱼首先聚在一起(0 4009),而后蜂巢石斑鱼-鲑点石斑鱼与点带石斑鱼聚在一起(0 4611),最后,蜂巢石斑鱼-鲑点石斑鱼-点带石斑鱼再与黑边石斑鱼聚在一起(0 4951)。在所研究的类群中,蜂巢石斑鱼和鲑点石斑鱼有较近的亲缘关系。

The restriction fragment length polymorphism(RFLP) of mitochondrial DNA(mtDNA) of Epinephelus spp.(E.merra,E.fario,E.awoara,E.akaara and E.septemfasciatus) was studied using 17 restriction endonucleases with 5-?and 6-bp recognition sites to construct their physical maps of mtDNA and establish a panel of molecular markers for the purposes of protection,exploitation and utilization.The samples were collected from the coastal area of Zhanjiang,Guangdong Province.These enzymes included BamHⅠ,BglⅠ,BglⅡ,DraⅠ,EcoRⅠ,EcoRⅤ,HindⅢ,KpnⅠ,MluⅠ,PstⅠ,PvuⅡ,SalⅠ,ScaⅠ,SmaⅠ,StyⅠ,XbaⅠ...

The restriction fragment length polymorphism(RFLP) of mitochondrial DNA(mtDNA) of Epinephelus spp.(E.merra,E.fario,E.awoara,E.akaara and E.septemfasciatus) was studied using 17 restriction endonucleases with 5-?and 6-bp recognition sites to construct their physical maps of mtDNA and establish a panel of molecular markers for the purposes of protection,exploitation and utilization.The samples were collected from the coastal area of Zhanjiang,Guangdong Province.These enzymes included BamHⅠ,BglⅠ,BglⅡ,DraⅠ,EcoRⅠ,EcoRⅤ,HindⅢ,KpnⅠ,MluⅠ,PstⅠ,PvuⅡ,SalⅠ,ScaⅠ,SmaⅠ,StyⅠ,XbaⅠ and XhoⅠ.mtDNA was extracted from the fresh liver tissue by applying a difference centrifugation procedures.The purified mtDNA was cleaved by single or double enzymes.Electrophorese of digested products was conducted through 0.7% agarose gels in 0.5 TBE running buffer at room temperature and 5 V/cm for 2 h.The size of mtDNA was estimated using the Gelpro32 software.The estimated size of mtDNA was(18.52±0.21) kb,(18.44±0.21) kb,(18.56±0.18) kb,(18.57±0.19) kb and(18.36±0.11) kb respectively for E.merra, E.fario, E.awoara, E.akaara and E.septemfasciatus,which almost accorded with the results that had been published of more than fifty other species of fishes.There were 20,17,16,12 and 13 cleavage sites detected for E.merra, E.fario, E.awoara, E.akaara and E.septemfasciatus with 10(including BamHⅠ,BglⅠ,BglⅡ,EcoRⅠ,EcoRⅤ,PstⅠ,SalⅠ,ScaⅠ,SmaⅠ and XhoⅠ),9(including BglⅠ,EcoRⅠ,EcoRⅤ,KpnⅠ,MluⅠ,PstⅠ,ScaⅠ,SmaⅠ and XhoⅠ),8(including BglⅠ,BglⅡ,EcoRⅠ,EcoRⅤ,MluⅠ,PstⅠ,SmaⅠ and XbaⅠ),7(including BamHⅠ,EcoRⅠ,EcoRⅤ,PstⅠ,PvuⅡ,SmaⅠ and XhoⅠ) and 7(including BglⅠ,BglⅡ,EcoRⅠ,EcoRⅤ,PstⅠ,PvuⅡ and XhoⅠ) restriction enzymes,respectively.According to these cleavage sites,the physical map of mtDNA from the five species was constructed.The enzymes BglⅡ and XhoⅠcan serve as diagnostic markers for identification of these five species.The cleavage sites of the enzymes BglⅡ and XhoⅠ showed no genetic diversity of intraspecies among these five species.There were 2,3,1,3 and 1 cleavage sites detected with enzyme BglⅡ.And the relevant restriction fragments lengths of mtDNA were 14.04 kb and 4.54 kb;8.19 kb,5.46 kb and 4.58 kb;18.47 kb;12.93 kb,2.93 kb and 2.63 kb;18.21 kb for E.merra, E.fario, E.awoara, E.akaara and E.septemfasciatus,respectively.There were 2,2,0,2 and 1 cleavage sites detected with the enzyme XhoⅠ.The relevant restriction fragments lengths of mtDNA were 16.76 kb and 1.85 kb;10.10 kb and 8.40 kb; 16.57 kb and 1.77 kb;18.21 kb for E.merra, E.fario, E.akaara and E.septemfasciatus respectively.

用识别5、6碱基序列的17种限制性内切酶,即BamHⅠ、BglⅠ、BglⅡ、DraⅠ、EcoRⅠ、EcoRⅤ、HindⅢ、KpnⅠ、MluⅠ、PstⅠ、PvuⅡ、SalⅠ、ScaⅠ、SmaⅠ、StyⅠ、XbaⅠ和XhoⅠ,对南海海域石斑鱼属(EpinephelusBloch)野生蜂巢石斑鱼(E.merra)、鲑点石斑鱼(E.fario)、青石斑鱼(E.awoara)、赤点石斑鱼(E.akaara)和七带石斑鱼(E.septem-fasciatus)的线粒体DNA(mtDNA)进行RFLP分析。测算出蜂巢石斑鱼、鲑点石斑鱼、青石斑鱼、赤点石斑鱼和七带石斑鱼的mtDNA分子大小分别为(18.52±0.21)kb、(18.44±0.21)kb(、18.56±0.18)kb、(18.57±0.19)kb和(18.36±0.11)kb。通过单、双酶切分析,分别构建了蜂巢石斑鱼10种酶共20个酶切位点、鲑点石斑鱼9种酶共17个酶切位点、青石斑鱼8种酶共16个酶切位点、赤点石斑鱼7种酶共12个酶切位点和七带石斑鱼7种酶共13个酶切位点的酶切物理图谱。BglⅡ和XhoⅠ两种内切酶的酶切谱带可作为鉴别这5种石斑鱼的...

用识别5、6碱基序列的17种限制性内切酶,即BamHⅠ、BglⅠ、BglⅡ、DraⅠ、EcoRⅠ、EcoRⅤ、HindⅢ、KpnⅠ、MluⅠ、PstⅠ、PvuⅡ、SalⅠ、ScaⅠ、SmaⅠ、StyⅠ、XbaⅠ和XhoⅠ,对南海海域石斑鱼属(EpinephelusBloch)野生蜂巢石斑鱼(E.merra)、鲑点石斑鱼(E.fario)、青石斑鱼(E.awoara)、赤点石斑鱼(E.akaara)和七带石斑鱼(E.septem-fasciatus)的线粒体DNA(mtDNA)进行RFLP分析。测算出蜂巢石斑鱼、鲑点石斑鱼、青石斑鱼、赤点石斑鱼和七带石斑鱼的mtDNA分子大小分别为(18.52±0.21)kb、(18.44±0.21)kb(、18.56±0.18)kb、(18.57±0.19)kb和(18.36±0.11)kb。通过单、双酶切分析,分别构建了蜂巢石斑鱼10种酶共20个酶切位点、鲑点石斑鱼9种酶共17个酶切位点、青石斑鱼8种酶共16个酶切位点、赤点石斑鱼7种酶共12个酶切位点和七带石斑鱼7种酶共13个酶切位点的酶切物理图谱。BglⅡ和XhoⅠ两种内切酶的酶切谱带可作为鉴别这5种石斑鱼的RFLP标记。

 
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