程序性降温 programmed cryopreservation(2)   programmed cryopreservation      To investigate the effect of cryopreservation on the viability of tenocytes, and compare the difference between normal cryopreservation and programmed cryopreservation.      目的研究深低温冷冻方法对肌腱细胞活性的影响,比较程序性降温和普通深低温冷冻法对腱细胞活性的影响。 短句来源      Methods Twenty-four 21-day-old Sprague-Dawley rats were randomly divided into 3 groups, control group (A) , normal cryopreservation group (B), and programmed cryopreservation group (C) .      方法纯种SD大鼠24只(出生21 d),随机分为3组,取双侧跟腱。 新鲜肌腱对照组(A),常规深低温冷冻组(B),程序性降温深低温冷冻组(C)。 短句来源   相似匹配句对      On Procedural Defense      程序性辩护研究 短句来源      On the Procedural Sanction of the Criminal Actions      刑事诉讼的程序性制裁 短句来源      The Comparison of Cooling Methods of Central High Fever      中枢性高热降温方法的比较 短句来源      A Programmed Cooling Method Used for the Preparation of Homograft      程序降温法用于同种瓣膜的制备 短句来源      A program adopting the H.Cross iterative method is suggested.      该程序采用H. 短句来源 查询“程序性降温”译词为用户自定义的双语例句     我想查看译文中含有：的双语例句 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。   programmed cryopreservation One sample from every patient was cryopreserved by means of the technique of programmed cryopreservation: programmed freezing of human parathyroid tissue at a controlled rate of -1°C/min to -80°C prior to transfer to liquid nitrogen (group II). 例句来源       A successful solution to this problem could be achieved by programmed cryopreservation with cryoprotectant. 例句来源       Programmed cryopreservation with 15% dimethyl sulfoxide (cryoprotectant) was used. 例句来源       These results confirm that programmed cryopreservation with cryoprotectant does not alter the molecular or geometric configuration of collagen or elastic fibers. 例句来源                Objective To show the effects of different methods on preservation of cord blood hematopoietic cells below zero degree. Methods MNCs were isolated with Ficoll, and the dectection included methods of observing Trpanblue dying, killing activity to K562 cells, and CFU total number. Results We were able to benefit from controlling the v/v of DMSO and gradually lowering the temperature of frozen cells. Conclusion The results can help us to select a good method when a cord blood bank is established.             目的 探讨不同冻存方法对脐血造血细胞的影响。方法 冻存前后脐血单个核细胞通过台盼蓝拒染率、对K562杀伤活性及总集落形成数来进行检测。结果 控制冻存保护剂浓度和程序性降温可减小对脐血造血细胞的损害。结论 该研究可为脐血建库提供一种较好的冻存方法。 文摘来源          To investigate the effect of cryopreservation on the viability of tenocytes, and compare the difference between normal cryopreservation and programmed cryopreservation. Methods Twenty-four 21-day-old Sprague-Dawley rats were randomly divided into 3 groups, control group (A) , normal cryopreservation group (B), and programmed cryopreservation group (C) . Tenocytes from the 3 groups were cultured with the same method. Primary and secondary passage cells were observed under phase microscope. The growth curve o...             目的研究深低温冷冻方法对肌腱细胞活性的影响,比较程序性降温和普通深低温冷冻法对腱细胞活性的影响。方法纯种SD大鼠24只(出生21 d),随机分为3组,取双侧跟腱。新鲜肌腱对照组(A),常规深低温冷冻组(B),程序性降温深低温冷冻组(C)。采用相同的方法对3组肌腱细胞进行细胞培养。相差显微镜观察原代和传代后细胞的生长,绘制细胞的生长曲线,考察细胞的活性;对细胞进行成纤维细胞染色、胶原染色和对细胞进行形态观察(扫描电镜);水解法定量分析细胞培养基中羟脯氨酸浓度的变化,检测细胞合成胶原的能力。结果原代细胞培养时A组细胞的生长速度快于B组和C组 (P<0．01),C组细胞的生长速度快于B组(P<0．01),这种生长速度的差异在细胞传代后消失。细胞的形态学和组织学符合成纤维细胞形态。3组细胞培养基中羟脯氨酸浓度变化的差异无统计意义(P> 0．05)。结论经深低温冷冻处理的肌腱中仍存在具有活性的腱细胞,但数量显著少于新鲜肌腱中活细胞的数量。应用计算机控制程序性慢速降温方法处理的肌腱其活细胞的数量有所提高,但仍低于新鲜肌腱中活细胞的数量。 文摘来源       相关查询: 程序性 程序性权利 程序性事实 程序性监督 程序性缺陷 实证性程序 程序性电刺激 程序和平性 程序性裁判 程序移植性 程序性辩 程序主体性  对查询结果不满意

CNKI小工具 在Springer中查有关的英文论文 在知识搜索中查有关程序性降温的内容 在数字搜索中查有关程序性降温的内容 在概念知识元中查有关程序性降温的内容 在学术趋势中查有关程序性降温 的内容

CNKI主页 |  设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务

2008 CNKI－中国知网

京ICP证040431号 互联网出版许可证 新出网证(京)字008号 北京市公安局海淀分局 备案号：110 1081725  2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社