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The PEVE genome codes for two helicases, one homologous to the circovirus replication initiation protein (Rep) and one corresponding to the helicase domain of papillomavirus E1.
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The system consists of plasmids that are all based on the broad-host-range lactococcal replicon pWV01 which has been deprived of its gene encoding the replication initiation protein RepA.
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Activation of the α origin depends on the R6K replication initiation protein π.
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N-terminal truncated forms of the bifunctional π initiation protein express negative activity on plasmid R6K replication
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The replication initiation protein π of the Escherichia coli plasmid R6K is a dual regulator in the control of plasmid copy number, functioning both as a specific initiator and inhibitor of replication.
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