助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   rna斑点杂交 的翻译结果: 查询用时:1.279秒
图标索引 在分类学科中查询
所有学科
生物学
肿瘤学
基础医学
园艺
药学
内分泌腺及全身性疾病
中药学
更多类别查询

图标索引 历史查询
 

rna斑点杂交     
相关语句
  rna dot blot
     OPN mRNA expression was detected with RNA dot blot and RT-PCR.
     采用RNA斑点杂交和 RT-PCR技术检测OPN mRNA的表达。
短句来源
     RNA dot blot analyses with RPA3 (a cDNA clone of rice Phy A) as a probe showed: the abundances of Phy A mRNA in top leaves of Nongken 58S and "Nongken 58" were obviously higher in SD than those in LD at the end of dark phase of 5 d and 10 d photoperiod treatments.
     以光敏色素A基因(phy A)的特异性片段RPA3作探针,用RNA斑点杂交的方法对叶片中Phy A mRNA丰度进行分析的结果表明,光周期处理5d和10d时,两品种水稻的Phy A mRNA丰度都是SD处理的比LD的高,而且SD下农垦58S Phy A mRNA的丰度均比“农垦58”的高。
短句来源
     TGF β 1 mRNA in the lesioned ischemic cortex and hippocampus was monitored on different times after HIBD by reverse transcription polymerase chain reaction(RT PCR) and DNA RNA dot blot hybridization. Semiquantitative analysis of RT PCR product of TGF β 1 was done with internal control of glyceraldehyde 3 phosphate dehydrogenase (GAPDH) by White/UV Transilluminator.
     利用反转录聚合酶链反应 (RT- PCR)和 DNA- RNA斑点杂交技术检测新生大鼠 H IBD后不同时间海马、皮质 TGF-β1 基因转录水平的表达 ,经凝胶成像及分析系统扫描 RT- PCR扩增产物 ,以内参照甘油醛 - 3-磷酸脱氢酶 (GAPDH)半定量分析 TGF- β1m RNA的动态变化。
短句来源
     The present study employed RNA dot blot hybridization and immunohistochemistry to explore the expression of basic fibroblast growth factor in normal prostate tissue (NP) ,benign prostate hyperplasia(BPH) and prostatic carcinoma(Pca), aiming to study possible role of b-FGF.
     本实验应用RNA斑点杂交技术和免疫组织化学的方法检测正常前列腺(NP)、良性前列腺增生(BPH)和前列腺癌(Pca)组织中碱性成纤维第四军医大学硕士学位论文细胞生长因子(b一FGF)的表达,旨在探讨其在良性前列腺增生(BPH)和前列腺癌(Pca)中的可能作用,能否成为一种前列腺癌的新的肿瘤标记物。
短句来源
     The vector was transfected into human ovarian cancer cell HO-8910 and the positive clone was screened by G418,VEGF expression was confirmed by RNA dot blot.
     用此真核表达载体转染人卵巢癌细胞HO 8910 ,G418筛选获得阳性克隆 ,RNA斑点杂交鉴定HO 8910细胞中VEGF表达。
短句来源
更多       
  rna dot hybridization
     Methods The gene expression of Th1/Th2 cytokines in human tumor cells was detected by RT PCR and RNA dot hybridization using IL 2 and IFNγ as Th1 type cytokine genes, IL 4, IL 10 and IL 13 as Th2 type cytokine genes.
     方法以IL-2、IFNγ代表Th1类细胞因子,IL-4、IL-10和IL-13代表Th2类细胞因子,用逆转录聚合酶链反应和RNA斑点杂交检测人肿瘤细胞中Th1/Th2类细胞因子的基因表达。
短句来源
     By determination of RNA dot hybridization, the amount of mRNA produced in the transcription of argSΔ4, argSΔ5 and argSΔ6 was about 2—3 times than that of the wild type argS, argSΔ7 and argSΔ8 . This indicated that the deletion of a 19 nt sequence (AATAGTGAAAACGGCAATA) located between -52 nt and -70 nt of the gene increased the transcription of argS .
     通过RNA斑点杂交测定发现 ,argSΔ4、argSΔ5和argSΔ6的mRNA转录量为argS及argSΔ7的 2到3倍。
短句来源
     When hybridizing with Ⅱ 32A,Ⅱ 32B and Ⅱ you 949 by OPJ18 1000 probe,we found OPJ18 1000 which had effects on rice Ⅱ 32A and Ⅱ you 949 cytoplasmic male sterility in transcription had not the same effects on Ⅱ 32B.Though RNA dot hybridization was negative,OPJ18 1400 had a conservative domain of seven base pairs DNA sequence:5′ TTCCCTC 3′,which was homologous recombination hotspot domain in atp6 gene.
     用OPJ18 10 0 0作探针与Ⅱ 32A、Ⅱ 32B、Ⅱ 优 94 9的mtRNA斑点杂交结果表明 ,除Ⅱ 32B外 ,都在转录水平上对水稻雄性不育有一定影响。 虽然OPJ18 14 0 0对应的RNA斑点杂交呈阴性 ,但其DNA序列中存在一个七碱基 5′ TTC CCTC 3′的保守系列。
短句来源
     However, by RNA dot hybridization the amount of mRNA produced in the transcription of parg-leuS was about 5 times that of the wild type le uS, and was similar to that of pKK-leuS, suggesting that the promoter of argS is very strong.
     但是 ,RNA斑点杂交却发现从 parg leuS转录出来的leuSmRNA是野生型leuS转录的 5倍 ,几乎与从 pKK leuS中转录出来的量差不多 ,说明argS的启动子很强 ,接近于trp lac。
短句来源
     The conditions for detecting tobacco mosaic virus (TMV) RNA in tobacco leave extract by RNA dot hybridization using a radioactive probe containing the cDNA of 3' terminal RNA of TMV strain OM have been studied.
     用普通烟草花叶病毒OM株3′-端约2kb的cDNA为探针,探索了用RNA斑点杂交法对烟草组织中烟草花叶病毒RNA进行检测的条件。
短句来源
更多       
  rna dot blot hybridization
     TGF β 1 mRNA in the lesioned ischemic cortex and hippocampus was monitored on different times after HIBD by reverse transcription polymerase chain reaction(RT PCR) and DNA RNA dot blot hybridization. Semiquantitative analysis of RT PCR product of TGF β 1 was done with internal control of glyceraldehyde 3 phosphate dehydrogenase (GAPDH) by White/UV Transilluminator.
     利用反转录聚合酶链反应 (RT- PCR)和 DNA- RNA斑点杂交技术检测新生大鼠 H IBD后不同时间海马、皮质 TGF-β1 基因转录水平的表达 ,经凝胶成像及分析系统扫描 RT- PCR扩增产物 ,以内参照甘油醛 - 3-磷酸脱氢酶 (GAPDH)半定量分析 TGF- β1m RNA的动态变化。
短句来源
     The present study employed RNA dot blot hybridization and immunohistochemistry to explore the expression of basic fibroblast growth factor in normal prostate tissue (NP) ,benign prostate hyperplasia(BPH) and prostatic carcinoma(Pca), aiming to study possible role of b-FGF.
     本实验应用RNA斑点杂交技术和免疫组织化学的方法检测正常前列腺(NP)、良性前列腺增生(BPH)和前列腺癌(Pca)组织中碱性成纤维第四军医大学硕士学位论文细胞生长因子(b一FGF)的表达,旨在探讨其在良性前列腺增生(BPH)和前列腺癌(Pca)中的可能作用,能否成为一种前列腺癌的新的肿瘤标记物。
短句来源
     Detection of Laminin mRNA in Human Primary Liver Cancer Tissue by in situ Hybridization and RNA Dot Blot Hybridization
     用原位杂交和RNA斑点杂交法检测人原发性肝癌层粘蛋白mRNA
短句来源
     The RZ expression was observed by Southern, RNA dot blot hybridization and flow cytometry (FCM) .
     用Southern印迹杂交、RNA斑点杂交和流式细胞仪(FCM),观察RZ基因在HL-60细胞内的表达。
短句来源
     METHODS Primary epidermal keratinocytes were isolated, cultured in serum free medium, transfected by Lipofectamine and selected with antibiotic G418. The expression and activity of IL 2 were assessed with techniques of DNA dot blot, RNA dot blot, hybridization in situ , immunohistochemistry, Western blot and MTT colorimetric assay.
     方法 分离原代皮肤角质形成细胞 ,无血清培养基培养 ,L ipofectamine介导转染 ,G41 8筛选 ,DNA斑点杂交、RNA斑点杂交、原位杂交、免疫组织化学、Western blot分析及 MTT法检测 IL- 2在细胞中的表达及分泌情况 .
短句来源
更多       
  rna hybridization
     Method: sixpairs of Primers specific for the IL-2, IL-4, IL-10, IL-13, IFNr and B-actin were designed. A Sensitive and rapid method for detection of Th1/Th2cytokine genes by RT-RCR was established. Meanwhile another method: the RNA hybridization with sir cDNA probes were established.
     方法:设计6对用于IL-2、IL-4、IL-10、IL-13、IFNr和β-actin基因扩增的引物,建立可用于Th1/Th2相关细胞因子检测的RT-PCR技术,同时用切口平移法制备6种细胞因子的cDNA探针,建立RNA斑点杂交技术。
短句来源
     Results:The RT-PCR reaction and RNA hybridization was used to test five kinds of Cytokine mRNAs expressed 20 tumor cell lines on mRNAs were expressed in eleven Cell lines; The mRNAs in eight cell lines.
     结果:用RT-PCR和RNA斑点杂交技术检测20种肿瘤细胞株,发现11/20为Th2型细胞因子表达,8/20为Th0型,1/20未检测到细胞因子。
短句来源

 

查询“rna斑点杂交”译词为其他词的双语例句

 

查询“rna斑点杂交”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  rna dot blot
The expression of three heat shock proteins (HSPs)-HSP90α, HSP70, HSP27 in cells obtained from 22 patients with leukemia, K562 erythroleukemia cell line, and normal blood cells was observed by means of RNA dot blot analysis.
      
Quantitative evaluation of this difference by RNA dot blot analysis revealed that the amount of GFAP mRNA expressed in CL3 was about 1/5 and in CL4 about 1/10 the amount expressed in U-251 MG, CL1, and CL2.
      
By RNA dot blot analysis, MDR1 transcripts were detectable in 11 of 34 specimens.
      
Northern and RNA dot blot analysis demonstrate that the expression of these clones is higher by at least a factor of 100-500 in leaves as compared to roots and tubers.
      
Northern and RNA dot blot analyses revealed enhanced accumulation of psam1 RNA after inoculation with G.
      
更多          
  rna dot hybridization
The expression of vascular endothelial growth factor (VEGF) was detected by RNA dot hybridization and immunohistochemical method.
      
RNA dot hybridization indicates that the plasmid is transcribed in mitochondria.
      
  rna dot blot hybridization
RNA dot blot hybridization further confirmed the results after excluding false positives.
      
Both Virtual Northern blot and RNA dot blot hybridization has showed such different expression.
      
  rna hybridization
A new RNA of about 900 nt was found in the virions of cocksfoot mottle virus (CfMV) and in infected plants by RNA hybridization and RT-PCR.
      
The potential regions of intermolecular RNA-RNA hybridization, or clinger fragments, in 16S rRNA, which are complementary to the sites frequently occurring in mRNAs and tRNAs, were found.
      
A comparative localization of Na+,K+-ATPase and ouabain-sensitive H+,K+-ATPase in rat skin was performed using in situ RNA hybridization and immunohistochemistry.
      
DNA -RNA hybridization was carried out with the probes and RNAs isolated from different strains.
      
The temporal and spatial expression patterns of calmodulin mRNA in the developing tobacco anthers were investigated byin situ RNA hybridization, using digoxigening-labeled anti-RNA probe.
      
更多          
  其他


The conditions for detecting tobacco mosaic virus (TMV) RNA in tobacco leave extract by RNA dot hybridization using a radioactive probe containing the cDNA of 3' terminal RNA of TMV strain OM have been studied. These conditions include the homology between the TMV strains isolated from Yunnan and Shanghai and the strain OM, the method for extracting viral RNA from tobacco leave and for adsorbing the extracted viral RNA onto nitrocellulose filter efficiently, the material interrupting the adsorption of viral...

The conditions for detecting tobacco mosaic virus (TMV) RNA in tobacco leave extract by RNA dot hybridization using a radioactive probe containing the cDNA of 3' terminal RNA of TMV strain OM have been studied. These conditions include the homology between the TMV strains isolated from Yunnan and Shanghai and the strain OM, the method for extracting viral RNA from tobacco leave and for adsorbing the extracted viral RNA onto nitrocellulose filter efficiently, the material interrupting the adsorption of viral RNA onto nitrocellulose filter and the hybridization of viral RNA with probe, and the speciality, sensitivity of the RNA dot hybridization method for detecting TMV-RNA in tobacco leave.

用普通烟草花叶病毒OM株3′-端约2kb的cDNA为探针,探索了用RNA斑点杂交法对烟草组织中烟草花叶病毒RNA进行检测的条件。这些条件包括用分子杂交法观察云南烟区和上海烟草上分到的烟草花叶病毒与OM株的同源性,从烟草组织中提取烟草花叶病毒的几种方法的比较,使RNA有效地固定在硝酸纤维素滤膜上的方法,烟草组织中是否有干扰RNA固定和杂交的物质,斑点杂交方法检测烟草花叶病毒的特异性、灵敏度等。

Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA of dengue virus 2 RNA. Using the [a-~(32)p] dCTP labelled pEH 920 DNA as the probe, dengue virus RNA in the supernatants of infected C6/36 mosquito cells have been detected by DNA-RNA dot-spot hybridization. The results show that the probe strongly reacted with dengue 2 virus RNA though it also cross-hybridized with dengue 1, 3 and 4 virus RNA. The preliminary experiments show that the probe can at least detect...

Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA of dengue virus 2 RNA. Using the [a-~(32)p] dCTP labelled pEH 920 DNA as the probe, dengue virus RNA in the supernatants of infected C6/36 mosquito cells have been detected by DNA-RNA dot-spot hybridization. The results show that the probe strongly reacted with dengue 2 virus RNA though it also cross-hybridized with dengue 1, 3 and 4 virus RNA. The preliminary experiments show that the probe can at least detect 625 TCID_(50) of dengue 2 virus RNA. The Chinese isolates of dengue 2 virus can also be detected by the probe.

新近制备了大量纯化的pEH920 DNA,该质粒DNA插入了登革病毒2型核酸片段的互补DNA。以[a-~(32)P]dCTP按缺口转译法标记pEH 920 DNA作为探针,以感染病毒的蚊细胞c_6/36培养上清作标本,应用DNA-RNA斑点杂交法检测了登革病毒核酸。结果显示同位素标记探针(pEH 920)与登革病毒2型标本反应最强,具有一定的型特异性。但与其它血清型登革病毒也呈一定交叉反应。初步探讨了探针的敏感性,至少可检出TCID_(50)625的登革病毒2型核酸。

Four of positive clones were obtainedby three runs of screening the cDNA libraryof human liver with rabbit antihumanapolipoprotein (apo) CⅢ IgG. One of themwas identified to be the positive clone ofapoCⅢcDNA by restriction endonucleasemap studids of tbe cDNA insert fragmentand by Western blot analysis of the ex-pression products. The apoCⅢcDNA-γgtllrecombinant DNA prepared from the posi-tive clone can be used in RNA--dot blotanalysis as apoCⅢ cDNA probe. And theapoCⅢcDNA clone can be also used to pro-duced apoCⅢ...

Four of positive clones were obtainedby three runs of screening the cDNA libraryof human liver with rabbit antihumanapolipoprotein (apo) CⅢ IgG. One of themwas identified to be the positive clone ofapoCⅢcDNA by restriction endonucleasemap studids of tbe cDNA insert fragmentand by Western blot analysis of the ex-pression products. The apoCⅢcDNA-γgtllrecombinant DNA prepared from the posi-tive clone can be used in RNA--dot blotanalysis as apoCⅢ cDNA probe. And theapoCⅢcDNA clone can be also used to pro-duced apoCⅢ peptide under the induction ofisopropy1--β--D--thiogalactoside. The apoCⅢpeptide, after purification,has lot of usein further studies.

用载脂蛋白(apo)CⅢ抗体探针对正常人肝cDNA库进行筛选,得到4个阳性克隆。经限制性内切酶谱对其cDNA插入片段及Western blot杂交时,其表达产物的鉴定表明筛选出的4个阳性克隆为apoCⅢcDNA克隆。以此克隆制备了含apoCⅢcDNA的重组DNA,经RNA-斑点杂交分析说明,此重组DNA可用来作为apoCⅢcDNA探针。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关rna斑点杂交的内容
在知识搜索中查有关rna斑点杂交的内容
在数字搜索中查有关rna斑点杂交的内容
在概念知识元中查有关rna斑点杂交的内容
在学术趋势中查有关rna斑点杂交的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社