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诱导产物
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  induced products
     LOW DOSE RADIATION INDUCED PRODUCTS IN MOUSE SPLEEN AND THEIR BIOLOGICAL ACTIVITY
     小鼠脾细胞中低剂量辐射诱导产物及其生物学活性
短句来源
     Low dose radiation (LDR) induced products were extracted from mouse spleen and its biological activity was preliminarily detected. The mouse spleen cells were sonicated and the induced products in the lysate were obtained with centrifugation.
     采用不同的低剂量辐射 (LDR)剂量、LDR诱导产物用量、照射方式、照射后蛋白提取时间为处理因素 ,观测诱导产物生物学活性的变化。
短句来源
     Results showed that the transformation ability of mouse spleen cells was significantly enhanced after stimulation of these cells with LDR-induced products. And the above-mentioned changes in experimental conditions could influence the activity of the induced products to different extents.
     实验证实 ,经 5- 15cGyLDR后 2 - 16h都可从小鼠脾脏细胞中提取得到LDR诱导产物 ,诱导产物具有生物学活性 ,可刺激脾脏细胞转化 ,但是 ,其活性受到多种因素的影响。
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  “诱导产物”译为未确定词的双语例句
     Conclusion: TP could significantly down-regulate TNFα-induced COX-2, iNOS expression and production of PGE2, NO in human RASF, which is associated with the suppression of NF-κB activity.
     结论 :TP可显著抑制 TNFα刺激的 RASF COX- 2和 i NOS的表达及其诱导产物PGE2和 NO的生成 ,这?
短句来源
     About 15-20 mg of recombinant protein was purified from 1 L of culture supernatant of recombinant E. coli by Ni~2+-NTA affinity column chromatography,and reached a purity of more than 80%.
     通过Ni2+-NTA亲和柱,从1L诱导产物中纯化出约1520mg重组蛋白,纯度在80%以上。
短句来源
     OBJECTIVE To assess the mutation and expression of the p53 gene in in anaplastic large-cell lymphoma (ALCL).
     目的:通过检测P53基因及P53蛋白诱导产物P21,MDM2在间变性大细胞瘤(ALCL)中的突变和表达。
短句来源
     The objective proteins were purified by 12%SDS-PAGE firstly and subsequently by 5%~20% gradient SDS-PAGE. The antisera against the CPs were raised in mice and their specificities were determined by Western blot.
     通过12%SDS-PAGE和5%~20%梯度SDS-PAGE二次制备电泳纯化诱导产物,免疫小鼠,获得高纯度的抗CP血清。
短句来源
     The result of a some 4.0KD specific protein band appearing in 12% SDS-PAGE electrophoresis demonstrated that rabbit NP-1 gene expressed successfully in E.
     诱导产物经12%的聚丙烯酸胺凝胶电泳进行检测,结果显示有分子量约为4.0KD的特异蛋白质条带出现,说明兔NP-1基因在大肠杆菌中得到成功表达。
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  相似匹配句对
     Apoptosis Induced by Natural Products on Hepatic Stellate Cell
     天然产物诱导肝星状细胞凋亡的研究
短句来源
     After induction,a 52 kDa new protein band appeared in SDS\|PAGE.
     S D S P A G E 显示一52 k Da 的诱导产物
短句来源
     in troducing stimulus;
     诱导刺激;
短句来源
     Smu.
     产物Smu.
短句来源
     laevis and no products were gained.
     laevis无产物
短句来源
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  induced products
HPLC analysis of γ-irradiation-induced products of tryptophan in peptides and lysozyme
      
Physical analysis of 51 DSB-induced products arising from multiple recombinational repair events suggested that hDNA formation is generally limiting, but that some hDNA regions may extend more than 600 bp.
      
The morphology and crystallography of the stress-induced products have been examined in detail.
      
Stress-induced products in a Ti-14.8 V alloy deformed in tension
      
Three LPS-induced products are shown that were reduced or completely blocked in the presence of SB202190.
      
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This papaer deals with the immunological response of three species of silkworms and the results are summarized as follows.1.The pupae of Antheraea pernyi, Philosamia cynthia ricini and Bombyx mori could all be induced to producing antibacterial substances in the haemolymph by injection with Escherichia coli or treatment with ultrasonic waves. Both methods induced the pupae of the same species of silkworms to yield the same products, but different antibacterial spectra appeared in the different species.2.Similar...

This papaer deals with the immunological response of three species of silkworms and the results are summarized as follows.1.The pupae of Antheraea pernyi, Philosamia cynthia ricini and Bombyx mori could all be induced to producing antibacterial substances in the haemolymph by injection with Escherichia coli or treatment with ultrasonic waves. Both methods induced the pupae of the same species of silkworms to yield the same products, but different antibacterial spectra appeared in the different species.2.Similar antibacterial substances were induced by E. coli at different developmental stages of B. mori. The ratios of antibacterial substances induced by E. coli in B. mori pupae were different in different sexes and the period of response was also different in the different strains of B. mori.3. In addition to the common antibacterial protein bands, a product which was more basic and moved faster in electrophoresis (PAGE) than the others was induced by injection with poly I:C. Separation and identification of this new substance are now in progress.All of our results indicate that the inducible antibacterial substances in the silkworms are nonspecific for the inducers and corresponding relationship cannot be found.

本文报道:1.蓖麻蚕Philosamia cynthia ricini、家蚕Bombyx mori及柞蚕Antheraea pernyi注射大肠杆菌Escherichia coli或超声波处理均能诱导血淋巴产生抗菌物质。同一蚕种诱导产物相同,不同蚕种间差异明显。2.家蚕不同发育阶段的个体诱导产生的抗菌物质基本相同;不同性别的家蚕蛹的诱导产物的相对量有差异;不同品种家蚕蛹对诱导的应答潜伏期也不尽相同。3.注射聚肌胞核苷酸(PolyI:C)诱导家蚕血淋巴产生一种明显地不同于其它诱导源诱导的抗菌物质,此物质在酸性聚丙烯酰胺凝胶电泳中泳动速度较快。 本研究的所有结果均表明,诱导绢丝昆虫产生抗菌物质的诱导源是非专一性的,即诱导源与诱导产物之间无对应的关系。

The structural effect on the induced asymmetric addition of dialkyl phosphites to C=N double bond was investigated by MNDO and MM2 (85) calculations. As appro ximation, the structure of products was used as model of the transition state of the reaction. The influence of structure of dialkyl phosphites, the type and concentration of catalysts as well as the solvent effect on the induced direction and the d. e. values can be well rationalized by these calculation results. These data also reveal that the Induced...

The structural effect on the induced asymmetric addition of dialkyl phosphites to C=N double bond was investigated by MNDO and MM2 (85) calculations. As appro ximation, the structure of products was used as model of the transition state of the reaction. The influence of structure of dialkyl phosphites, the type and concentration of catalysts as well as the solvent effect on the induced direction and the d. e. values can be well rationalized by these calculation results. These data also reveal that the Induced direction and the de values are substantially governed by the formation of coordination species resulted from interactions between catalyst and the π electrons of benzene ring connected with asymmetric inductive substituents. If the catalyst ia unable to coordinate with benzene nuclei, the principal product usually has R-configu ration, otherwise, the S-configuration product will be predominant.

本文应用MNDO和MM2(85)研究了亚磷酸二酯对碳氮双键的诱导不对称加成反应的结构效应,催化剂种类和浓度以及溶剂等因素对诱导方向及de值的影响.结果表明,反应的诱导方向及诱导效果强烈地受到在反应过渡态中诱导基团的苯环与催化剂配合作用的影响.当苯环不参与配合时,诱导产物以R构型为主;当苯环参与配合时,诱导产物以S构型为主.该模型解释了催化剂种类和浓度、溶剂及酯烷基等结构效应.

The RNA genome was purified from beet necrotic yellow vein virus (BNYVV) (G1 strain) and full length double-stranded cDNA of RNA3 was synthesized by using the RNA3 as template, and oligo(dT)15 and RNA3-specific primer. The double-stranded cDNA was cloned into pGEM3zf( + ) and generated recombinant plasmid pGB533. Determination of the nucleotide sequence of the cDNA fragment shows that RNA3 is 1776nt in length and it contains three open reading frames (ORFs), which encode two proteins with molecular weights of...

The RNA genome was purified from beet necrotic yellow vein virus (BNYVV) (G1 strain) and full length double-stranded cDNA of RNA3 was synthesized by using the RNA3 as template, and oligo(dT)15 and RNA3-specific primer. The double-stranded cDNA was cloned into pGEM3zf( + ) and generated recombinant plasmid pGB533. Determination of the nucleotide sequence of the cDNA fragment shows that RNA3 is 1776nt in length and it contains three open reading frames (ORFs), which encode two proteins with molecular weights of 25kD, 4.6kD and a polypeptide with 59 amino acids in length called protein N. The nucleotide sequence shows homology of 96.8% with that of F2 strain. As for the deduced amino acid sequence, ORF P25, ORFN and ORF P4.6 have homology of 93.6%, 93.2% and 92.3%, respectively. Analysis of the amino acid sequence indicate a zinc finger in P25, and also extremely acidic and basic domanis. Protein N is extremely hyd-rophobic. 5' and 3' extremeties of RNA3 are highly conserved. A double hairpin motif is present at the 3' extremety of RNA3, which is essential during encapsida-tion and replication of the virus. After site-directed in vitro mutagenesis, outhor have succeed in expression of the β-gataclosidase-P25 fusion protein in E. colt.

以G1株系的甜菜坏死黄脉病毒(BNYVV)RNA3为模板,以Oligo(dT)_(15)为引物合成cDNA第一链,以RNA3特异引物合成cDNA第二链。然后将双链DNA克隆在pGEM3zf(+)载体中。通过限制性内切酶图谱分析,构建了五个亚克隆,完成全序列1776bp的序列测定(不含Poly(A)尾巴)。对核苷酸序列和推测的氨基酸序列分析结果发现,RNA3含三个开放阅读框架(ORF),分别编码三个多肽,即P25、P4.6和蛋白N。与法国F2株系RNA3在核昔酸水平上的同源率达96.8%,相应的CRF F25、ORF P4.6和ORFN编码多肽的同源率分别为93.6%、93.2%和92.3%。P25含一个钉指结构(zinc finger),和酸性及碱性区域;蛋白N为疏水蛋白。ORF以外的两端序列高度保守,且3'端可形成双发卡结构(double hairpin motif),说明这段序列在病毒侵染或复制中有十分重要的作用。进一步将RNA3 eDNA体外定点突变后使P25 ORS 与卢-半乳糖苷酶基因位于同一阅读框架以表达卢-半乳糖苷酶和P25融合蛋白,在诱导产物中检测到卢-半乳糖苷酶与P25的融合蛋白...

以G1株系的甜菜坏死黄脉病毒(BNYVV)RNA3为模板,以Oligo(dT)_(15)为引物合成cDNA第一链,以RNA3特异引物合成cDNA第二链。然后将双链DNA克隆在pGEM3zf(+)载体中。通过限制性内切酶图谱分析,构建了五个亚克隆,完成全序列1776bp的序列测定(不含Poly(A)尾巴)。对核苷酸序列和推测的氨基酸序列分析结果发现,RNA3含三个开放阅读框架(ORF),分别编码三个多肽,即P25、P4.6和蛋白N。与法国F2株系RNA3在核昔酸水平上的同源率达96.8%,相应的CRF F25、ORF P4.6和ORFN编码多肽的同源率分别为93.6%、93.2%和92.3%。P25含一个钉指结构(zinc finger),和酸性及碱性区域;蛋白N为疏水蛋白。ORF以外的两端序列高度保守,且3'端可形成双发卡结构(double hairpin motif),说明这段序列在病毒侵染或复制中有十分重要的作用。进一步将RNA3 eDNA体外定点突变后使P25 ORS 与卢-半乳糖苷酶基因位于同一阅读框架以表达卢-半乳糖苷酶和P25融合蛋白,在诱导产物中检测到卢-半乳糖苷酶与P25的融合蛋白已在大肠杆菌中得到表达。

 
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