A 280 bp DNA band that presented RASSF1A expression at transcriptional level and a 40 kD protein band that presented RASSF1A expression at protein level were detected by RT-PCR and Western blot respectively in the experimental group and there were no corresponding bands in the control group.
SDS-PAGE analysis revealed that the GST-MIH1 fusion protein was highly expressed after the induction with IPTG for 4 h. A protein band of 34 kD Mr appeared on SDS-PAGE gel.
By analysis of SDS-PAGE, a new protein band, the recombinant hOP-1 mature peptide about 16×10 3 was found and made up of 40% of the total bacteria proteins.
There were seven major protein bands always in sonicate and freeze-thawing antigens of Helicobac-ter pylori by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. They were 71kd,62kd,58kd,51kd,29kd,22kd,and 12kd.
The SDS-PAGE indicated that in the venom there were approximately 12 protein bands, in which most of them were less than 100 kD with 43.5, 25.9 and 20.1 kD bands in the highest concentration, while in the Dufour's there were approximately 15 protein bands, in which the most abundant bands were 121.4, 77.0, 51.5, 42.7 and 36.5 kD.
SDS-PAGE analysis exhibited that the major protein of the ECPs comprised of three abundant protein components with the molecular weight about 35 ku,45 ku, 53 ku, respectively.
SDS PAGE分析显示,胞外产物的SDS PAGE图谱主要包括35 ku,45 ku,53 ku 3个主要蛋白质条带。
Specificity of immunostaining was confirmed by preincubation of HRP-labeled anti-SERT antibodies with excess of corresponding antigen, which resulted in disappearance of protein band staining.
SDS-polyacrylamide gel electrophoresis gel gave a single protein band and the molecular mass of purified enzyme was estimated to be approximately 138 kDa.
In addition, purification and concentration of the major HSA protein band (monomer) of 68 kD from the other oligomeric protein bands were achieved by use of a two-membrane system in a simple electroelution apparatus.
By using a Phospho-Ser/Thr PKA substrate antibody, we have detected an increased phosphorylation of 150, 200 and 220-kDa protein bands when starfish spermatozoa treated with EJ.
The integral tegumental membrane proteins have been obtained from the crude tegument membrane preparation by the extraction and removal of the water soluble proteins and other peripheral protein components with sodium lauryl sulphate. Five' membrane proteins were identified by SDS-PAGE electrophoresis with the following molecular weight: Ⅰ. 64,000; Ⅱ. 58,000; Ⅲ. 39,000; Ⅳ. 27,000 and Ⅴ. 18,700. The membrane proteins of Ⅰ, Ⅲ and Ⅳ are glycoproteins. Among these membrane proteins two components can be recogni...
Duing 1980-1982, six maize parental lines of "zymogram purity" and their hybrid were used as experimental materials. The grains at 5, 10-days and the endosperms, embryos at 15, 20, 25, 30, 35, 40, 45 and 50-days after pollination were taken, and dried to constant weight. The results indicated that the course of the dry matter accumulation is a "S" curve and that of average weight increasing is a "two peak" curve during the development of endosperms, embryos and grains. The course of development can be divid...
After electrophoretic transfer of antigens from polyacrylamide gel tonitrocellulose, the membrane was used for determining the specificity of themnnoclonal antibodies against thyroglobin by ELISA.The immunological activity ofthemonoclonal antibodies can be tested and verified on the blot by the appearancerown bands at the position of the corresponding antigens.