The constructed structure was then inserted into plant vector pCAMBIA3301 and the recombinant plasmid pCAMBIA3301-fad2i was obtained,in which,bar gene is a selection marker and gus gene is a detection marker.
Upon the optimized Agrobacterum-mediated transformation systems mentioned above, the herbicide-resistant gene bar has proved to be successfully incorporated into genomes of the Cynodon dactylon after PCR assay of hpt gene and bar gene, GUS histochemical assay and hpt resistance evaluation.
A rolled-leaf mutant was obtained in a T-DNA( containing bar gene and Ds element) insertion population, which consist of transgenic japonica rice Zhonghua 11 mediated by Agrobacterium tumefaciens.
This study deals with the polymerase chain reaction (PCR) detection technology of the marker gene bar , CaMV 35 S promoter and Nos terminator DNA sequence in both crude transgenic rice and its processed food stuffs.
The barstar gene coding for the barnase inhibitor was cloned from Bacillus amyloliquefaciens genomic DNA. Chimeric genes containing the 5′ regulatory region (-1300 to +3) of TA 29 gene fused to the barstar coding region, and the CaMV 35S promoter fused to the herbicide (PPT)-resistant gene bar were constructed into pBI121.1, resulting in plasmid pBBS.
Upon the optimized Agrobacterum-mediated transformation systems mentioned above, the herbicide-resistant gene bar has proved to be successfully incorporated into genomes of the Cynodon dactylon after PCR assay of hpt gene and bar gene, GUS histochemical assay and hpt resistance evaluation.
(4) The gene bar was transferred into 4 elite upland rice cultivars using particle bombardment and lots of plantlets with herbicide tolerance were obtained.
Four green plants were finally regenerated from 388 immature embryos bombarded with plasmid pARN6 containing rice chitinase gene (RC24) and pD containing reporter gene (gus) as well as selective marker gene (bar) on the medium supplemented with 2mg/L Basta.
Recombinant expression vector was constructed in which the r-PA(reteplase, r-PA) and bar genes were inserted under the control of promoters of SV40 and CaMV 35S respectively.
Three systems (gradation, delayed, and regeneration) for in vitro selection of transgenic wheat tissue using the bar gene, providing resistance to the herbicide phosphinothricin (PPT), were compared.
violaceus plants that had been previously transformed by a vector containing the maize transposon system Spm/dSPm with bar gene located within the nonautonomous transposon.
Northern blotting hybridizations showed that the bar transcript was most abundant in cells grown in 2 mol/L NaCl, and less abundant in 0.5 mol/L NaCl, indicating that expression of the bar gene was induced at high salinity.
We utilized gene transfer technology for genetic perennial ryegrass improvement, efficient regeneration, and Agrobacterium-mediated transformation of phosphinothricin acetyltransferase gene (bar).
The unselectable gene (uidA) and the selectable gene (bar), on two separate plasmids, were transferred to maize (Hi-II derivative) by particle bombardment of embryogenic calli or suspension cells.
A 640-bp DNA fragment upstream to the start codon was employed to drive the expression of the reporter protein sGFP or a dominant selectable marker, the gene bar (resistance to ammonium glufosinate).
Hartog) were electroporated in the presence of plasmid pEmuGN and/or pEmuPAT, which contained the reporter gene gus and selectable marker gene bar, respectively.
The engineered male sterile gene barnase together with the mark gene bar were transformed to the isolated cotyledon of a sesane variety Yuzhi 4 by microprojectile bomebardment.The transformed cotyledon was cultured under darkness for 12 days then converted into dark 1ight alternative conditions on the culture media MS+5mg·L -1 6-BA+1mg·L -1 IAA+1mg·L -1 ABA+5mg·L - AgNO 3 Resistant calli were selected on herbicide Basta 2mg.L -1 11 anti Basta plants were obtained.Southern blot of 3...
The barstar gene coding for the barnase inhibitor was cloned from Bacillus amyloliquefaciens genomic DNA. Chimeric genes containing the 5′ regulatory region (-1300 to +3) of TA 29 gene fused to the barstar coding region, and the CaMV 35S promoter fused to the herbicide (PPT)-resistant gene bar were constructed into pBI121.1, resulting in plasmid pBBS. Cotyledonary petioles of a “Double Low” variety of oilseed rape, “5-4”, were transformed with A. tumefaciens strain LBA4404 harboring pBBS....
Total RNA was isolated from ripen fruit of tomato.Ethylene-forming enzyme(EFE) cDNA was synthesized and recombined into Bluescript Vector at EcoRV site to clone EFE gene with reverse-transcription polymerase chain reaction(RT-PCR).The inserted fragment was analyzed with restriction endonuclease and subclones were constructed,meanwhile the whole cDNA of EFE was sequenced,then EFE antisense gene and BAR gene with 35S promoter and nos-terminater were inserted into expression vector pSPROK.Finally,the plant exp...
基因bar
bar gene
查询“基因bar”译词为用户自定义的双语例句
相关查询:
2008 CNKI－中国知网