The results showed that the induction frequency of directly induced somatic embryos is highest when immature zygotic embryos are cultured on MS medium supplemented with 1mg/LIAA and 20mg/LBA.
Medium Y5: MS+2,4-D 4.0 mg/L+KIN 1.0 mg/L or medium B5 Ⅲ: B5+2,4-D 3.0 mg/L+KIN 0.1 mg/L+Phe 0.1 mol/L was confirmed optimum callus induction medium in which callus induction rate had reached to 100%.
培养基Y 5:M S+2,4-D 4.0 m g/L+K IN 1.0 m g/L或B 5Ⅲ:B 5+2,4-D 3.0 m g/L+K IN 0.1 m g/L+Phe 0.1 m o l/L为最佳诱导愈伤培养基,其诱导频率高达100%。
Haploid were induced from thick and thin rind melon, line 4810 and cultivar Xinfuyu, to which the induction rate of haploid were 0.55% and 0.63% respectively, when the dose was 300 Gy.
Endosperm of Passiflora edulis was used as explant. The callus induction rate (84.7%) was obtained when endosperm was cultured on MS supplemented with 0.2mg/L BA and 2.0 mg/L NAA.
The final induced frequency reached its highest point when using B 5 with 0.3 mg/L 2,4 D and 3 mg/L 6 BA as induced medium and MS with 3 mg/L 6 BA and 0.5 mg/L GA 3 as differentiated medium.
It was found that organs of sterile seedlings of Pueraria phaseoloides (Roxb.) Benth. formed Calli on MS medium contained 1 mg/L NAA and 1mg/L 2,4,5 T for 20 days, the callus induced frequency of cotyledon was higher than those frequency of leaves, stems, roots, embryonal axis and radicle .
The callus derived from their hypocotls initiated earlier,developed faster with a higher induced frequency. 0.3～0.5 cm,4 day hypocotls close to root or to cotyleden were better explants.
Tests of exogenous hormones and explant ages revealed: 1. Supplement with exogenous cytokinins alone could induce the explants excised from the pistils to regenerate the fruit like structures, and the highest induced frequency (50%) was obtained when 0 5 mg/L zeatin was supplemented. Exogenous auxin seems un necessary.
外源激素和外植体年龄的试验揭示：1．以雌蕊组织作外植体时，仅附加外源细胞分裂素就可以诱导果实状结构的再生，外源生长素似乎不是必需的，最高的诱导频率（50．0％ ）出现在仅附加玉米素0．5 m g／L的组合。
Theresults showed that the ability of callus formation from the mature embryo was popular easy in30 corn genotypes, but the induced frequency was greatly different between different genotypes,and callus quality were different between different genotypes.
The best result was obtained from the anthers cultured continuously at 7—10℃ for 120 hours on medium added with 2:6 mg/l IAA and kinetin after inoculation. Under these conditions the frequency of induction increased to 6.2%.
After one or two months, turned the cormels into the medium which was MS supplemented with 5mg/L NAA, 5mg/L BA and cultured in darkness. Tufty buds can be induced after one or two subcultures. The frequency of induction was as high as 74%.
Cefotaxime did not affect the induction frequency and growth of the embryogenic callus but enhanced its morphogenesis.
Ovary culture on media without charcoal resulted in a similar induction frequency.
Callus induction frequency ranged from 23-100%, with Mol7 having the highest frequency.
Callus induction frequency and plantlet production were highest (25.0% and 45.8%, respectively) for Thinopyrum ponticum (2N=70) (tall wheatgrass).
The addition of 60 μM dicamba (3,6-dichloro-o-anisic acid) to the kernel culture medium resulted in an induction frequency of 27-38% compared to 0% for controls on standard kernel culture medium.
The frequency of this mode exhibits a strong linear dependence in the pressure region from 5 to 18 GPa, whereas, beyond this region, the slope of the pressure-induced frequency shift is reduced by about a factor of two.
The Rayleigh phonon dispersion on Cu(100): A stress induced frequency shift
Pressure broadening widths and pressure-induced frequency shifts for the
A simple expression is obtained for pressure induced frequency shifts in the resonator, which is examined for design optimization.
In this paper, we put emphasis on the analysis the mechanism of the photon-induced frequency conversion β-BaB2O4 crystal inside a borate glass using femtosecond laser.
Brief heating of the fraction changed its capacity to produce organised mesodermal tissues, such as notochord and somite, and the frequency of induction of undifferentiated cells was reduced.
Differences in frequency of induction were observed among genotypes.
The estimated frequency of induction of invasive variants was smaller than 1 in 106 cells.
The high frequency of induction of vegetative shoots and flowers before roots with isatin suggests its utility in micropropagation from plant tissue cultures.
Medium containing 6-benzylaminepurine (BA) (8.9 μM)+NAA (0.54 μM); Zeatin (45.62 μM)+NAA (5.37 μM) or BA (8.9 μM) stimulated both organogenesis in callus (frequency of induction =50%) and semi-organized tissue in shoot buds.
诱导频率
induction frequency
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