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小鼠卵
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  mouse oocyte
     THE ROLE OF CHANGES IN INTRACELLULAR FREE CALCIUM LEVEL OF MOUSE OOCYTE DURING FERTILIZATION AND ARTIFICIAL ACTIVATION
     小鼠卵受精和人工激活过程中胞质游离Ca~(2+)变化及其在卵激活中的作用
短句来源
     Development of Reconstructed Mouse Oocyte from In Vivo Matured Ooplasm Transfered with Cumulus Cell Nucleus
     小鼠卵丘细胞核移植到体内成熟卵浆构成的重构卵的发育
短句来源
     The present experiment used cultured mouse cumulus enclosed oocyte (CEO) and denuded oocyte (DO) to study the mechanism of the regulation of gonadotrophin in mouse oocyte meiotic resumption.
     本实验利用卵母细胞的体外培养模型,将小鼠卵丘-卵母细胞复合体(CEO)和去卵丘卵母细胞(DO)在体外培养,系统研究了促性腺激素(FSH、hCG)诱导小鼠卵母细胞减数分裂的机制。
短句来源
     To observe the fertilization and development of reconstructed mouse oocyte derived from in vivo matured ooplasm transfered with mouse cumulus cell nucleus.
     【目的】观察小鼠卵丘细胞核移植到体内成熟卵浆构成的重构卵的发育。
短句来源
  “小鼠卵”译为未确定词的双语例句
     Cloning, Sequencing of mouse Zone Pellucida 3(mZP3) cDNA and Constructing mZP3 DNA vaccine
     小鼠卵透明带3(mZP3) cDNA的克隆、测序及mZP3 DNA疫苗的构建
短句来源
     Expression of Integrin α_5、α_6、β_1 Subunits on Human and Mouse Oocytes and Their Relationship with Maturation of Oocytes
     整合素α_5、α_6、β_1在人、小鼠卵的表达及其与卵成熟的关系
短句来源
     Results: ① MT at all doses of 0.1 g/L、0.02 g/L、0.4 g/L or 0.8 g/L inhibited the formation of PB1 in CEO cultured in maturation medium and had no effect on GVBD.
     结果 :① 0 .1g/L、0 .0 2g/L、0 .0 0 4 g/L及 0 .0 0 0 8g/L浓度的MT均能显著抑制小鼠卵丘卵母细胞复合体 (CEOs)自发成熟过程中第一极体 (PB1)的释放 (P <0 .0 1) ;
短句来源
     The results show MT at all doses of 0. 1mg/ml、0. 02mg/ml、0. 004mg/ml or 0. 0008mg/ml inhibited the formation of PB1 in CEOs cultured in maturation medium and ha no effect on GVBD.
     结果表明:0.1mg/ml、0.02mg/ml、0.004mg/mg及0.0008mg/ml浓度的MT均能显著抑制小鼠卵丘卵母细胞复合体(CEO_s)自发成熟过程(P<0.01)。
短句来源
     CD_(44) and Inter-α-Trypsin Inhibitor Expressionin the Cumulus-Oocyte Complexes of Mice
     小鼠卵丘-卵母细胞复合体CD_(44)和间α胰蛋白酶抑制剂的表达
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  相似匹配句对
     Production and Study of Mouse Reconstructed Oocytes with Somatic Cells
     小鼠重构的制作及研究
短句来源
     1. Superovulation, natural mating, collection of fertilized eggs;
     1.小鼠超排及取
短句来源
     than that in WT mice.
     通小鼠
短句来源
     The G-banding Patterns in Mouse (Maa musculus) Ghromosomes
     小鼠G显带核型
短句来源
     The nucleus is egg-shaped.
     细胞核圆形。
短句来源
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  mouse oocyte
A map of the mouse oocyte has been constructed utilizing early and mid/late pachytene stages.
      
Morphology and transcriptional activity of mouse oocyte chromosomes
      
We have examined pachytene and early diplotene mouse oocyte chromosomes through the light microscope using sections of plastic-embedded material and air dried spreads stained with either silver nitrate or methyl green pyronin.
      
Chromatin behaviour under influence of puromycin and 6-DMAP at different stages of mouse oocyte maturation
      
We have examined the dynamics of the localisation of the polo-like kinase 1 (Plk1) during maturation of the mouse oocyte.
      
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Blastomeres at various stages be-fore blastula of the early mouse em-bryos were observed under transmis-sion electron microscope.A great deal of highly ordered lattice-like struc-tures distributed throughout the cy-toplasm of blastomeres of the early mouse embryos.They were seen in oocyte as early as the stage of early primary follicle.Microscopic sec-tions of ovary and early embryos were stained with toluidine blue for show-ing the changes of cytoplasmic ba-sophilia during oocyte development and early cleavage.The...

Blastomeres at various stages be-fore blastula of the early mouse em-bryos were observed under transmis-sion electron microscope.A great deal of highly ordered lattice-like struc-tures distributed throughout the cy-toplasm of blastomeres of the early mouse embryos.They were seen in oocyte as early as the stage of early primary follicle.Microscopic sec-tions of ovary and early embryos were stained with toluidine blue for show-ing the changes of cytoplasmic ba-sophilia during oocyte development and early cleavage.The cytoplasm of oocytes in primordial follicle with a single layer of flattened granulosa cells contained fibers and granules in-tensely stained with toluidine blue.The cytoplasm of oocytes in advan-ced growing follicle and of blastomeres at early cleavage stages exhibited a homogeneously pale feature after to-luidine blue staining.Consequently,there was a corresponding relation between the appearance of lattice-like structures and the change of cy-toplasmic basophilia during oocytegrowth.The weakening of cytoplas-mic basophilia of oocytes in advanced growing and mature follicles and of blastomeres at early cleavage stages presumably related to the incorpora-tion of ribosomes into lattice-like stru-ctures.Each chain of lattices was composed of a series of granules avera-ged 210-230 A in diameter.At 4-cell,8-cell and 16-cell stages many parallel chains among lattice-like struc-tures were highly curved in a semi-circle or fingerprint feature,and the chain at the lesser curvature (con-cave) edge of curved parallel chains was transformed into a cluster of granules in a spiral array (polyribo-some).This change represented the transition of ribosomes from an in-active form to an active one.The observations present here support the idea that the granules in the chain of lattice-like structure of mouse oocy-tes and early embryos are the ribo-somes in a storage form.These ri-bosomes progressively become active during development of the early em-bryos.

对小鼠受精卵至16细胞阶段的早期胚胎细胞做了电镜观察。这些阶段的细胞质中含有大量规则排列的网架状结构,在切面上表现为平行链。每条链是由颗粒物质串连而成,颗粒的直径约为210-230埃。自4细胞阶段以后,网架状结构中有的平行链弯曲成半圆形或指纹状,小弯侧之链转变成螺旋排列的多核糖体。这种转变反映出核糖体由储备状态变为活动状态。网架状结构在卵母细胞发育中形成。生长卵母细胞的细胞质嗜碱性减弱与网架状结构的形成和多核糖体数量的减少有着相应的关系。早期胚胎细胞的弱嗜碱性,可能仍系因含大量网架状结构和多核糖体较少所致。根据超微结构形态变化和染色反应表明,网架状结构是小鼠卵母细胞和早期胚胎细胞中核糖体的储备形式。这些母体来源的核糖体在早期胚胎发育中逐渐动用。

According to the morphology,number,and arrangment of the granularcells,and the presence or absence of follicular antrums in the follicles.The oo-cytes in the mature ovaries of the two breeds(Northeastern Native and Harbinwhite sows)were divided into five developing stages:Ⅰ,Ⅱ Ⅲ(including Ⅲ_1、Ⅲ_2),Ⅳ,Ⅴ。Electron microscopic findings were shown as follows:A few short microvilli began to emerge on the oolemma of oocytes stage 11.The zone pellueida appeared around oocytes stage 111.There were cortical granules(CGs)in...

According to the morphology,number,and arrangment of the granularcells,and the presence or absence of follicular antrums in the follicles.The oo-cytes in the mature ovaries of the two breeds(Northeastern Native and Harbinwhite sows)were divided into five developing stages:Ⅰ,Ⅱ Ⅲ(including Ⅲ_1、Ⅲ_2),Ⅳ,Ⅴ。Electron microscopic findings were shown as follows:A few short microvilli began to emerge on the oolemma of oocytes stage 11.The zone pellueida appeared around oocytes stage 111.There were cortical granules(CGs)in the oocytes of the two breeds.At first CGs were produced by the Golgi complex(GO)secretive surface nearthe nucleus in stage 11.At this time,CGs assumed the form of concentrated vesic-ular or dark granules in the mutivescular bodies.Thereafter,they migrated gradu-ally into the cortical region together wich GO,Mitochondria(Mi),etc.CGs mostly were ball-shaped and elliptical.They were differentiated into darkgranules and light granules according to their electron density.The diameter and number of CGs varied with size ot cocytes.The signifi-cant linear positive correlation(P<0.01)exsised between the averag numberof CGS and the average diameter of oocytes in various developing stages.while the oocytes were ovulating,the number of CGs reached the maximum.in themThere were not significant differences in genesis,size,morphology,numberand distribution of CGs between the two breeds.There were Annuli lamellae in the oocytoplasm in stage Ⅲ_1.The dominant Golgi complexes were found in the oocytes from stage 1 to Ⅳ.They appeared as the form of clusters of small vesioulae together with an arrayof cisternae.At the stage when the oocytes were synthesizing nutritive materials,there was an increase in the number of Mi,GO and rough endoplasmic reticulum(RER),and always the partial coalescence of Mi and RER,of GO and RERhad been found.This phenomenon probably reflected their functional relation-ships.In stage Ⅴ,GO were almost disappearance,RER were strikingly diminished,Mi changed uniformly small and sphere.But,lipid drops were even larger andmore than those of stage 111 and Ⅵ.Furthermore,the total area of lipid drops in one section was about 2/5 of the area of the whole cytoplasm.It was likelythat the large number of accumalation of the lipid drops was one of the charac-teristics in swine's oocytes.There was a first polar body in the perivitellinespace of this developing stage.A ccording to the morphological findings of submicroscopic level,we conclu-ded that the cytoplasm and oolemma of the oocytes in stage V had matured comple-tely.Noticeably,these differed greatly from the oocytes of rabbits and mice,which need continuous maturation in the oviducts.

本研究根据卵泡内颗粒细胞的形态、数量和卵泡腔的有无,把东北民猪、哈尔滨白猪卵巢内的卵母细胞划分成五个发育阶段:Ⅰ、Ⅱ、Ⅲ(包括Ⅲ_1、Ⅲ_2)、Ⅳ、Ⅴ。电镜观察发现:第Ⅱ阶段卵母细胞质膜开始产生少量、短的微绒毛。继后,微绒毛长大,密度增加。透明带于第Ⅲ阶段的卵母细胞开始形成。两品种猪的卵母细胞中均有皮质颗粒(CGs)存在。最初,CGs 由第Ⅱ阶段卵母细胞近核区的高尔基复合体(Go)分泌面产生。此时,CGs 呈浓缩小泡和多泡小体中暗色颗粒的形式存在。其后,它们和 Go、线粒体(Mi)等逐步迁移到细胞质的皮质区。CGs 大多呈球形。根据它们的电子致密度可分为暗粒和明粒。CGs 的直径和数量随着卵母细胞体积而变化。在各发育阶段的卵母细胞中,CGs 平均数量和卵母细胞平均直径增长之间呈非常显著的强相关(P<0.01)。当卵母细胞将排卵时,CGs 达到最大数量。两品种猪之间,在 CGs 发生、大小、形态、数量和分布上均无明显差异。第Ⅲ_1阶段卵母细胞中有环层板产生。从第Ⅰ到第Ⅳ阶段的卵母细胞中,我们都发现了占优势的 Go,它们由小泡集团和膜性囊组成。当卵母细胞制造营养物质的时候,Mi、Go 和粗面内质网(RER)出现一...

本研究根据卵泡内颗粒细胞的形态、数量和卵泡腔的有无,把东北民猪、哈尔滨白猪卵巢内的卵母细胞划分成五个发育阶段:Ⅰ、Ⅱ、Ⅲ(包括Ⅲ_1、Ⅲ_2)、Ⅳ、Ⅴ。电镜观察发现:第Ⅱ阶段卵母细胞质膜开始产生少量、短的微绒毛。继后,微绒毛长大,密度增加。透明带于第Ⅲ阶段的卵母细胞开始形成。两品种猪的卵母细胞中均有皮质颗粒(CGs)存在。最初,CGs 由第Ⅱ阶段卵母细胞近核区的高尔基复合体(Go)分泌面产生。此时,CGs 呈浓缩小泡和多泡小体中暗色颗粒的形式存在。其后,它们和 Go、线粒体(Mi)等逐步迁移到细胞质的皮质区。CGs 大多呈球形。根据它们的电子致密度可分为暗粒和明粒。CGs 的直径和数量随着卵母细胞体积而变化。在各发育阶段的卵母细胞中,CGs 平均数量和卵母细胞平均直径增长之间呈非常显著的强相关(P<0.01)。当卵母细胞将排卵时,CGs 达到最大数量。两品种猪之间,在 CGs 发生、大小、形态、数量和分布上均无明显差异。第Ⅲ_1阶段卵母细胞中有环层板产生。从第Ⅰ到第Ⅳ阶段的卵母细胞中,我们都发现了占优势的 Go,它们由小泡集团和膜性囊组成。当卵母细胞制造营养物质的时候,Mi、Go 和粗面内质网(RER)出现一个数量上的增加。并屡见 Mi 和 RER、Go 和 RER 相互连接的情况。这反映了它们的机能关系。第Ⅴ阶段的卵母细胞中,Go 几乎全部消失,RER 显著减少,Mi 一律变圆变小。但是,脂滴比第Ⅲ、Ⅳ阶段卵母细胞中的脂滴更大、更多。此时,脂滴约占胞质的2/5;脂滴的大量积聚是猪卵母细胞的特征之一。在卵周隙中有第一极体。根据超微结构的形态学所见,我们认为第Ⅴ阶段的猪卵母细胞质和质膜已经最终成熟。显然,这与排卵后还需在输卵管内进一步成熟的家兔、小鼠卵母细胞大不相同。

We report a study on the effects of millimeter-microwave irradiation on the enzymatic activity of mouse blastomere in vitro and in vivo, the source of microwave being 36.11 GHz, 8mm, and 2-4 mW/cm2 in frequency, wave length and power density respectively. The results show that the enzymatic activity on the surface of blastomere cells has bit influence. The AKPase, ATPase and -5'-Nase of blastomere cells irradiated in vitro were reduced significantly. But AKPase activity of blastomere cells irradiated in vivo...

We report a study on the effects of millimeter-microwave irradiation on the enzymatic activity of mouse blastomere in vitro and in vivo, the source of microwave being 36.11 GHz, 8mm, and 2-4 mW/cm2 in frequency, wave length and power density respectively. The results show that the enzymatic activity on the surface of blastomere cells has bit influence. The AKPase, ATPase and -5'-Nase of blastomere cells irradiated in vitro were reduced significantly. But AKPase activity of blastomere cells irradiated in vivo was reduced only a little. All the changes are discussed in the article.

作者报道用毫米波(频率36.11GHz,波长8mm,功率密度2~4mW/cm~2)辐照在体及体外小鼠卵胚,结果证明它可以影响卵胚细胞表面一些酶类活性。体外照后卵胚的碱性磷酸酶、三磷酸腺苷酶及5′-核苷酸酶活性均明显降低。在体照后三磷酸腺苷酸及5′-核苷酸酶活性明显降低,而碱性磷酸酶活性降低不明显。文章对这些酶类活性改变的影响作了讨论。

 
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