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胚胎学观察
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  embryological observation
     AN EMBRYOLOGICAL OBSERVATION ON APOSPORY IN MALUS TORINGOIDES (REHD.) HUGHES
     变叶海棠(Malus toringoides(Rehd.)Hughes)无孢子生殖的胚胎学观察
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     EMBRYOLOGICAL OBSERVATION ON THE APOMICTIC RICE ApⅢ(SHUANG 13)
     多胚水稻ApⅢ(双13)的胚胎学观察
短句来源
     THE EMBRYOLOGICAL OBSERVATION OF THE SEED DEVELOPMENT OF POPULUS ADENOPODA
     响叶杨(Populus adenopoda Maxim)种子发育的胚胎学观察
短句来源
     A study on Induction, Identification,and Utilization of Trisomic Plants in Upland Cotton Ⅲ. Embryological Observation of Female Gametophyte Development
     陆地棉三体的诱发、鉴定及其利用研究──Ⅲ雌配子发育的胚胎学观察
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     CYTOLOGICAL AND EMBRYOLOGICAL OBSERVATION ON THE CROSS OF AUTOTRIPLOID x DIPLOID IN RICE
     水稻同源三倍体与二倍体杂交的细胞胚胎学观察(简报)
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  “胚胎学观察”译为未确定词的双语例句
     Cyto embryological Study on Emasculated Florets of Genotype HS 86-11 in Wheat(Triticum aestivum)
     基因型HS86-11去雄穗细胞胚胎学观察
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     OBSERVATION AND STUDY ON EMBRYOLOGY OF CHIMONOBAMBUSA MARMOREA
     寒竹胚胎学观察与研究
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     Anatomical observations and embryological studies were conducted for rice material C1001.The results showed that twin-embryos existed at a very low frequency in hybrid caryopse of C100lA×C1001 B.The major type of apomixis in rice C1001 is adventitious embryo which originated from nucellar cell.
     对水稻C1001成熟种子进行形态解剖观察和各发育时期胚珠细胞胚胎学观察。 获得以下结果:C100lA×C1001B的杂种后代种子中存在低频率的双胚。
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     Embryologyic development of apomixis in Malus toringoides(Rehd. ) H. (3x)was stud-ied.
     对3x变叶海棠(M.toringoides(Rehd.)H.)无融合生殖作了胚胎学观察
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     1. The result of embryonic observation in W3338. The total of embryo sac are 374, the embryo sac of W3338 was typical polygonum type.
     1.W3338的胚胎学观察:总的观察了374个子房,其胚囊属于典型的蓼型胚囊,受精前的313个子房中,有1个子房的胚囊存在异常现象,即在极核周围出现三个类卵细胞,占未受精子房总数的0.27%;
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  相似匹配句对
     OBSERVATION
     观察
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     OBSERVATION AND STUDY ON EMBRYOLOGY OF CHIMONOBAMBUSA MARMOREA
     寒竹胚胎学观察与研究
短句来源
     Embryology Observation on Drug Abortive Embryos at Early Stages
     药物流产早期胎儿的胚胎学观察
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     Embryology of Tussilago farfara (Asteraceae:Senecioneae)
     款冬的胚胎学
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     Methods24 healthy crossbred dogs were chosen.
     观察方法:
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Haploid rice plantlets were induced from cultured ovaries in our previously reported experiment.The present paper is an embryological study on this subject. Young flowers of two japonica cultivars were excised and cultured just in the same manner as before.Liquid medium used for float culture was N6+3% sucrose+0.125 ppm MCPA.The inoculated materials were checked to be at late uninucleate pollen stage which corresponded mainly to the uninucleate embryo sac stage,but as well as some 2- or 4-nucleate embryo sacs.Samples...

Haploid rice plantlets were induced from cultured ovaries in our previously reported experiment.The present paper is an embryological study on this subject. Young flowers of two japonica cultivars were excised and cultured just in the same manner as before.Liquid medium used for float culture was N6+3% sucrose+0.125 ppm MCPA.The inoculated materials were checked to be at late uninucleate pollen stage which corresponded mainly to the uninucleate embryo sac stage,but as well as some 2- or 4-nucleate embryo sacs.Samples were fixed at 2-3 days' intervals in aceto-methanol (1:3),stained in toto with diluted Ehrlich's hematoxylin and sectioned by paraffin method for microscopical observation. 4 days after inoculation most of the embryo sacs developed up to 8-nucleate stage with polarized differentiation of the egg apparatus,central cell and antipodals.From 7th day on,proembryos of various sizes and shapes appeared in the micropylar region of some embryo sacs; some consisted of meristematic cells,others were highly vacuolated. One-celled as well as linear multicellular suspensors atypical of in vivo zygote proem- bryos were observed.However,it was uncertain whether the proembryos originated from the egg cell,the synergids,or the differentiating egg apparatus as a whole.Another peculiar event occured during culture was the formation of endosperm-like free nuclei from the unfertilized polar nuclei in some embryo sacs.Sometimes the free nuclei were numerous and showed a tendency of ceil formation in localized areas.12-15 days after inoculation,the proembryos developed into microscopical calli with globular or pearlike shape,which continued enlarging to visible size with,naked eyes at about 18-24 th day. Further growth eventually led the calli protruding out the ovary wall beyond 32-35 th day. These observations indicate that the embryo sac,similarly as the pollen,can be in- duced to embryogenesis in vitro.This may open a new way to study the mechanism con- trolling gametophytic and sporophytic developmental pathways of embryo sac and pro- vide means for large-scale production of“embryo sac plants”in future.

未受精的水稻幼花在液体漂浮培养条件下,由子房内产生愈伤组织并分化了小植株。胚胎学观察证明愈伤组织是起源于胚囊内部而非子房壁与胚珠体细胞组织。观察到胚囊珠孔端的原胚发生及其形态上的多样性。还发现由未受精极核形成类似胚乳游离核的现象。讨论了胚囊离体孢子体发育的意义与特点。

Experiments were Carried out in 1979 on the Institute of Genetics, Academia Sinica.The results obtained are summarized as follows: When the inoculated N.tabacum were checked to be at uninuc- leate pollen stage Which corresponded mainly to the megaspore mot- her cell stage (possess 71.89%) as well as some was dyad stage or tetrad of megaspores stage.When 3 days after inoculated most of the ovules expanded. For embryological observations.Ovaries of N.tabacum (Copus yeusuheku No4),N.rustica and Tr.aestivum (Rf_1)...

Experiments were Carried out in 1979 on the Institute of Genetics, Academia Sinica.The results obtained are summarized as follows: When the inoculated N.tabacum were checked to be at uninuc- leate pollen stage Which corresponded mainly to the megaspore mot- her cell stage (possess 71.89%) as well as some was dyad stage or tetrad of megaspores stage.When 3 days after inoculated most of the ovules expanded. For embryological observations.Ovaries of N.tabacum (Copus yeusuheku No4),N.rustica and Tr.aestivum (Rf_1) were sampled at 2—4days intervals after inoculation,fixed in Carnoy's Fluid (1 part of glacial acetic acid plus 3 parts of ethanol) for 18—24h, stored in 70% ethanol,stained in toto for14—16h in Heidenhain's haematoxylin,washed thoroughly in several changes of tap water, each for 10 minutes.The stained materials were passed through aroutine procedure of paraffin method,sectioned to 4—6um,and after being dewaxed with xy101,mounted directly in Canada balsam. Embryological observations revealed that after inoculated the unpollinated ovaries of N.tabacum were found to contain embryo sacs of different stages.Induction the megaspore mother cell were carried meiosis in vitro and formed one megaspore.It went on mitosis of formation 2—8 nuclei as more irregular free nuclei.From about 7 days on,emhryogenesis took place mass of cells in some embryo sacs,It were developed into embryoids. The results obtained revealed that there are two ways of plantlet production:(1)Ovary haploid plant was derived from the macrospore without an intervening Callus phase.For example,the N.tabacum regenerated plantlets of haploid were derived from macrospore.(2) Ovary haploid plant was derived directly from the egg cell of mature embryo sac.For example,the Tr.aegtivum regenerated plantlets of haploid were derived from differentiating Callus.In same medium embryoids not were derived from Cullas. Finally,Plantlets were developed.

未传粉的子房离体培养后,通过细胞胚胎学的观察结果表明,胚囊中出现接种时不存在的各个发育时期,并发现未传粉烟草子房中含有2—8个游离核,有的胚囊含有更多个排列不规则的游离核。当培养7天时,胚胎发生,胚囊中形成细胞团(即原胚状体),它进一步发育成为球形胚状体。单倍体烟草和小麦植株的形成来自两条途径:(1)来自大孢子,由它直接进行多次分裂,经细胞团、胚状体各阶段发育形成植株。(2)来自卵细胞,经胚状体各阶段发育而形成的。并非起源于子房壁和胚珠体细胞组织。

Unpollinated young ovaries of lily[Lilium davidii var.willmottiae(Wilson)Rof- fill]were inoculated on modified MS medium and N_6 medium.Ovary cultures were incubated at 25—28℃,and ilhnninated with a fluorescent light of about 800—1200 Lux.Cultured ovaries gradually became thicker and longer after 10 days.The calli (about 6—12 mm in size)were produced after 40 days.The calli were then transferred to the differentiation medium.After 50 days,regeneration plantlets were formed.Em- bryoids were directly produced...

Unpollinated young ovaries of lily[Lilium davidii var.willmottiae(Wilson)Rof- fill]were inoculated on modified MS medium and N_6 medium.Ovary cultures were incubated at 25—28℃,and ilhnninated with a fluorescent light of about 800—1200 Lux.Cultured ovaries gradually became thicker and longer after 10 days.The calli (about 6—12 mm in size)were produced after 40 days.The calli were then transferred to the differentiation medium.After 50 days,regeneration plantlets were formed.Em- bryoids were directly produced from some ovaries,which then developed into plantlets. Observation of chromosome number of regeneration plants shows: 65.71% regeneration plants are haploid plants,34.29% are diploid.Embryological observation of ovary cul- ture shows that haploid plants are from megaspore tetrad,while diploid plants are pro- bably from nucellus cell.

取未授粉的百合[Lilium davidii var.willmottiae(Wilson)Roffill]子房,放入改良 MS和 N固体培养基中,在25—28℃的弱光下培养,10天后子房显著膨大,40天后可产生6—12mm 大小的愈伤组织。转移到分化培养基上40—50天可形成完整的植株。经根尖染色体倍性观察有65.71%的再生植株为单倍体植株,具12条染色体;其余34.29%的再生植株为二倍体植株。在于房培养过程中进行了胚胎学观察,发现在离体培养条件下百合子房出现了非正常发育途径,单倍体植株来源于大孢子四分体,而不是来源于卵细胞。同时在珠心细胞中观察到旺盛的分裂活动和细胞团的形成,这可能是二倍体植株的来源。

 
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