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重组植酸酶
相关语句
  recombinant phytase
     Specific activity of the rude recombinant phytase is 461575.8 U/mg.
     重组植酸酶粗酶比活力为461575.8 u/mg。
短句来源
     Studies on the partial enzyme characters of recombinant phytase showed that the recombinant phytase had two pH optima (2.5-3.0 and 5.0-5.5) and a temperature optimum 55℃, and with higher activity under the pH ranged from 4.5 to 6.0. The result of SDS-PAGE indicated that the molecular weight of the recombinant phytase is between 70kDa-97kDa.
     重组植酸酶部分酶学性质的研究结果表明:重组酶在pH值2.5一3.0和5.0一5.5时酶活性最高,且在pH4.5一6.5之间均有较高的酶活性,最适作用温度为55℃。 sDs一PAGE的结果显示重组植酸酶的分子量介于70kDa~97kDa之间。
短句来源
     Four hundred seven-day old Avian broiler chickens were used to evaluate the effect of 0,300,600 and 900 IU/kg recombinant phytase on the performance and phosphorus utilization in comparison with dicalcium phosphate supplemented diet.
     选用400只7日龄艾维茵雏鸡 ,研究玉米—豆粕型日粮中添加0 ,300 ,600 ,900IU/kg 重组植酸酶和标准水平磷酸氢钙对肉鸡生长性能和磷利用的效果。
短句来源
     Recombinant phytase evolved by DNA shuffling was purified by ultra-filtration and DEAE-Sepharose F.
     经DNA改组的重组植酸酶通过有机膜超滤、DEAE -SepharoseF .
短句来源
     Study of Enhancement of Optical Brightener VBL on Peroral Infection of Recombinant Phytase Virus
     荧光增白剂VBL增进重组植酸酶病毒食下感染率的研究
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  “重组植酸酶”译为未确定词的双语例句
     So using the expression vector , successfully constucted the gene engineering strains Ecoli. BL21-pET30a+-phyA and lactobacillus MG1363-pMG36e-phyA. Then we wished to obtain the phytase of mass high activity 、obearing acids and good stability .
     BL21(pET30a+-phyA)和lacto bacillus MG1363(pMG36e-phyA),以期得到大量高活性、耐酸性和热稳定性好的重组植酸酶,为开发含植酸酶的新型、多功能微生态制剂提供基础数据和科学依据。
短句来源
     The recombinant yeast strains were genetically stable. The expressed phytases had two pH optima (2.5~3.0 and 5.0~5.5) and a temperature optimum of 55℃, and were active at pH ranged from 4.5 to 6.0.
     重组植酸酶在pH值 2 5~ 3 0和 5 0~ 5 5时酶活性最高 ,且在pH4 5~ 6 5之间均有相当高的酶活性 ,最适作用温度为5 5℃。
短句来源
     phyA gene was isolated from pMD18T- phyA with restriction enzyme and directly subcloned into expression vector pET30a+ and pMG36e.
     得到电泳纯的重组植酸酶,其分子量约为50kDa,表达蛋白占大肠杆菌总蛋白的36.62%;
短句来源
     Clones expressing phytase were selected from 37 transformants. Twoimportant results were found by determining property of purified phytase. Atfirst, the expressed phytase retained approximately 48% of its activity afterbeing subjected to 85℃ for 10 minutes.
     热失活试验表明该重组植酸酶在85℃加热10分钟仍然有48%的活性剩余。
短句来源
     BL21-pET32a+-phyA and Pichia PMAD-Pmet32a+-phyA. Then we wished to obtain the phytase of mass high activity, obearing acids and good stability. And provided basic datas and science accordings for developing a new type of multi-functional ecological agent, which had much phytase.
     BL21-pET32a+-phyA表达载体和酵母PMADl6-pMET αA-phyA,以期得到大量高活性、耐酸性和热稳定性好的重组植酸酶,为开发含植酸酶的新型、多功能微生态制剂提供基础数据和科学依据,将为今后研究和开发多功能微生态制剂,实现畜牧业综合化、环保化发展等应用研究奠定坚实的基础。
短句来源
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  相似匹配句对
     The recombinant plasmid expressed well in E.
     从重组E.
短句来源
     Enzymology property evaluation of phytase separated from gene-engineering recombined yeasts
     基因工程重组酵母植酸酶性质研究
短句来源
     Expression of Recombinant Phytase in Transgenic Rice
     转基因水稻中重组植酸酶的表达
短句来源
     Reform of the Order
     秩序的重组
短句来源
     The Research Development of Phytase
     植酸酶的研究进展
短句来源
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  recombinant phytase
The recombinant phytase had one optimum pH (pH?4.5) and an optimum temperature of 50°C.
      
Phytase expression in transgenic plant was enhanced with a synthetic phytase gene according to the Brassica codon usage and an endoplasmic reticulum (ER) retention signal KDEL that confers an ER accumulation of the recombinant phytase.
      
These results suggest a commercial feasibility of producing a stable recombinant phytase in canola at a high level for animal feed supplement and for reducing phosphorus eutrophication problems.
      
Detailed analysis of immature seeds by immunofluorescence and electron microscopy showed that recombinant phytase carrying a signal peptide for entry into the endoplasmic reticulum was efficiently secreted from storage cotyledon cells.
      
The medium for recombinant phytase production by E.
      
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Four hundred seven-day old Avian broiler chickens were used to evaluate the effect of 0,300,600 and 900 IU/kg recombinant phytase on the performance and phosphorus utilization in comparison with dicalcium phosphate supplemented diet.The result indicated that body weight gain and phosphorus deposition in tibia were improved by supplementation of the phytase with a dosage dependent relationship,but the phytase could not replace phosphorus supplementation completely.Phytase supplementation reduced excreta nitrogen...

Four hundred seven-day old Avian broiler chickens were used to evaluate the effect of 0,300,600 and 900 IU/kg recombinant phytase on the performance and phosphorus utilization in comparison with dicalcium phosphate supplemented diet.The result indicated that body weight gain and phosphorus deposition in tibia were improved by supplementation of the phytase with a dosage dependent relationship,but the phytase could not replace phosphorus supplementation completely.Phytase supplementation reduced excreta nitrogen and phosphorus output and could relieve the pollution.

选用400只7日龄艾维茵雏鸡 ,研究玉米—豆粕型日粮中添加0 ,300 ,600 ,900IU/kg 重组植酸酶和标准水平磷酸氢钙对肉鸡生长性能和磷利用的效果。结果表明 ,肉鸡体增重和胫骨磷沉积随植酸酶添加剂量而提高 ,但添加植酸酶不能完全取代无机磷。添加植酸酶可减少肉鸡粪便氮磷排泄量 ,减轻环境污染。

The chromosomal DNA was extracted from Aspergillus nigerN14 strain by benzyl chloride. A pair of primers were designed and synthesized according to the full sequence of the phyA gene (GenBank Accession No. AY426977) and used to amplify the 1.4kb gene using the high-fidelity DNA polymerase Pyrobest. The amplified fragment was cloned into pMD18-T to generate the recombinant expression vector pPIC9K-phyA containing the phytase encoding gene free of the signal peptide and intron sequence. Linearized by XbaⅠ restriction...

The chromosomal DNA was extracted from Aspergillus nigerN14 strain by benzyl chloride. A pair of primers were designed and synthesized according to the full sequence of the phyA gene (GenBank Accession No. AY426977) and used to amplify the 1.4kb gene using the high-fidelity DNA polymerase Pyrobest. The amplified fragment was cloned into pMD18-T to generate the recombinant expression vector pPIC9K-phyA containing the phytase encoding gene free of the signal peptide and intron sequence. Linearized by XbaⅠ restriction enzyme, the recombinant plasmid pPIC9K-phyA was transformed into Pichia pastoris GS115 by electroporation. Two recombinant strains, named PP-N14-22 and PP-N14-44, of high level of expression producing phytase of 143 958.3u/mL and 148 908.3u/mL respectively under shake cultivation, were identifed by G418 resistance, enzyme activity test, PCR verification and SDS-PAGE analysis. The enzyme activity was 341.13 times and 352.86 times higher than that of the original strain (422u/mL). The recombinant yeast strains were genetically stable. The expressed phytases had two pH optima (2.5~3.0 and 5.0~5.5) and a temperature optimum of 55℃, and were active at pH ranged from 4.5 to 6.0.

从黑曲霉N14基因组DNA中扩增出植酸酶phyA基因表达片段 ,并将其克隆到pMD18 T载体中。以此片段构建了pPIC9K phyA重组表达载体 ,目的片段以正确的阅读框架插入到pPIC9K的多克隆位点EcoRⅠ和NotⅠ之间。重组表达载体经XbaⅠ线性化处理 ,电击转化毕赤酵母 ,经G4 18抗性筛选、酶活性测定、PCR鉴定和SDS PAGE分析 ,获得了两株产酶活性分别为 14 395 8 3u mL发酵液 (PP N14 2 2 )和 14 890 8 3u mL发酵液 (PP N14 4 4 )的高产工程菌 ,其酶活性分别是出发菌株酶活性 (4 2 2u mL)的 341 13倍和 35 2 86倍 ,重组酵母具有很好的遗传稳定性。重组植酸酶在pH值 2 5~ 3 0和 5 0~ 5 5时酶活性最高 ,且在pH4 5~ 6 5之间均有相当高的酶活性 ,最适作用温度为5 5℃。

Recombinant phytase evolved by DNA shuffling was purified by ultra-filtration and DEAE-Sepharose F.F chromatography. Its molecular weight was estimated to be 85 000 by SDS-PAGE. Its optimum pH and temperature for phytase activity were pH 4.5 and 40 ℃, respectively. The thermal stability of phytase could be increased by 1~3 times to the addition of materials such as MgSO 4,arabinose,fructose,galactose ,lactose,trehalose,sucrose and glucose(100 g L -1). The kinetic parameters for the hydrolysis of sodium phytate...

Recombinant phytase evolved by DNA shuffling was purified by ultra-filtration and DEAE-Sepharose F.F chromatography. Its molecular weight was estimated to be 85 000 by SDS-PAGE. Its optimum pH and temperature for phytase activity were pH 4.5 and 40 ℃, respectively. The thermal stability of phytase could be increased by 1~3 times to the addition of materials such as MgSO 4,arabinose,fructose,galactose ,lactose,trehalose,sucrose and glucose(100 g L -1). The kinetic parameters for the hydrolysis of sodium phytate were K m 0.11 mmol L -1 and V max 964 mmol L -1min -1 at 37 ℃ and pH 4.5. Its activity was not dependent on the existence of metal ions. The phytase activity was inhibited by EDTA, SDS,Cu 2+, Zn 2+, K + and activated by Mn 2+, Mg 2+, Fe 2+, Ba 2+,Ca 2+, Co 2+ ; however, Mg 2+ could be an inhibitor when its concentration was up to 10 mmol L -1. Fig 4, Tab 4, Ref 19

经DNA改组的重组植酸酶通过有机膜超滤、DEAE -SepharoseF .F离子交换层析两步纯化 ,其纯度可达90 %左右 .SDS -PAGE分析表明 ,重组植酸酶的分子量Mr 约为 85 0 0 0 .酶学实验结果表明 :该酶反应最适pH为 4 .5 ,最适温度为 4 0℃ ,Km为 0 .11mmolL-1,Vmax为 96 4mmolL-1min-1,植酸酶活性不依赖任何金属离子的存在 .向酶液中分别添加MgSO4、阿拉伯糖、果糖、半乳糖、乳糖、海藻糖、蔗糖及葡萄糖 (10 0 g/L)后 ,植酸酶在 90℃处理 10min的活性比对照增加了 1~ 3倍 ,说明这些物质为酶保护剂 ;在 37℃下以植酸钠为底物的SDS对酶活性具有强烈的抑制作用 ;金属离子如Cu2 + ,Zn2 + ,K+ 以及EDTA对酶活性具有较弱抑制作用 ;金属离子如Mn2 + ,Mg2 + 、Fe2 + 、Ba2 + 、Ca2 + 、Co2 + 低浓度时对酶活性有促进作用 ,当Mg2 + 浓度增加到 10mmolL-1时 ,则对酶活性起抑制效应 .图 4表 4参 19

 
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