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荧光方法
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  fluorescence method
     A new sodium resonance fluorescence lidar is set up, which is for measurements of Sodium density by resonance fluorescence method over 80~110km altitude.
     介绍了一台监测高空钠层的激光雷达,通过共振荧光方法探测80~110km高度的钠层密度。
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     Upon excitation by femtosecond laser with a pulse-width of 200 fs, both compounds exhibit strong blue two-photon excited fluorescence in THF with λ_(max)=484 nm for 1 and λ_(max)=440 nm for 2. Their two-photon absorption cross-sections obtained by two-photon fluorescence method are 425 GM (1, excited at 800 nm) and 116 GM (2, excited at 730 nm).
     在脉宽为 2 0 0fs的飞秒激光激发下 ,它们在THF中发出强的蓝色上转换荧光 ( 1:λmax=484nm ;2 :λmax=44 0nm) . 用双光子荧光方法测得它们的双光子吸收截面分别为 42 5GM(激发波长为 80 0nm)和 116GM(激发波长为 73 0nm)
短句来源
     Application of radioisotope multichannel X-ray fluorescence method to the study of modern stratigraphy
     多道X射线荧光方法在现代地层学研究中的初步应用
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     APPLICATIONOF FLUORESCENCE METHOD TO THE STUDY OF HYDROCARBON MIGRATION
     荧光方法应用于油气运移研究初探
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     THE STUDY OF A NEW METHOD FOR COPPER OREPROSPECTING──X-RAY FLUORESCENCE METHOD
     普查铜矿的新方法——X荧光方法研究
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  fluorometric method
     A new fluorometric method has been developed for the determination of trace chromium, based on the fluoresence quenching effect of Cr( Ⅵ )2. 6.7-trihydroxy-9 (3, 5-cli-bromo-4-hydroxyphenyl)fluorone (DBH-PF)-emulgent OP system.
     本文基于Cr(Ⅵ)-二溴羟基苯基荧光酮(DBH-PF)-OP体系的荧光熄灭效应,提出一种测定痕量铬(Ⅵ)的新荧光方法
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     The affinity of selected peptides were assayed by ELISA and the inhibitory activity to influenza virus A/Sydney/5/97 neuraminidase were detected by fluorometric method.
     用ELISA方法鉴定噬菌体克隆与靶分子的结合力,用荧光方法测定噬菌体克隆对流感病毒A/Sydney/5/97(H3N2)神经氨酸酶的抑制活性。
短句来源
     A new fluorometric method has been developed for the determination of trace tungsten,based on the fluorescence quenching effect of W(Ⅳ) 5' nitro salicylfluorone (5' N SAF) Tween 80 system.
     本文基于W(Ⅵ)-5′-硝基水杨基荧光酮(5′-N-SAF)-Tween-80体系的荧光熄灭效应,提出一种测定微量钨的新荧光方法
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     Direct Measurement of Nitric Oxide by Fluorometric Method.
     荧光方法直接测定一氧化氮
短句来源
     A new fluorometric method has been developed for the determination of trace chromium based on the fluorescence quenching effect of Cr (VI)lorophenyl fluorone CTMAB system.
     基于 Cr( VI)—邻氯苯基荧光酮 ( O-Cl-PF)— CTMAB体系的荧光熄灭效应 ,提出了一种测定痕量铬 ( VI)的新荧光方法
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  “荧光方法”译为未确定词的双语例句
     The Dynamical Study of the Reactions Ba+C_6H_5L p-C_6H_4INH_2、C_6H_5Br、m,p-C_6H_4BrCH_3 by Means of LIF and Molecular Beam
     用分子束和激光诱导荧光方法来研究Ba与p-C_6H_4INH_2、n-C_4H_9I、C_6H_5Br、m-C_6H_4BrCH_3、n-C_4H_9Br的反应
短句来源
     By using the method of Discharge Flow-Resonance Fluorescence, the reaction of OH+SO_2+M was studied.
     用放电流动-共振荧光方法(DF-RF)研究SO_2+OH+M反应。
短句来源
     The paper reported that the emission spectrum of CF2(3B1) carbene produced by the reaction of O(3p) + CF2 = CFCl(or CF2 = CFBr) has been observed and the CFH carbene produced by the reaction of O(3p) + CF2 = CH2 has been identified by laser induced fluorescence spectrum, respectively for the first time.
     在O(~3P)和CF_2=CFBr(或CF_2=CFBr)反应中,首次观察到反应生成的CF_2(~3B_1)的发射光谱,而在O与CF_2=CH_2反应中,用激光诱导荧光方法检测到CFH卡宾,确定了在此反应中CFH卡宾的生成,这一结果未见文献报道.
短句来源
     The study of SiH_4/N_2 gas discharge plasma with LIF
     激光诱导荧光方法对SiH_4N_2混合气体放电等离子体的研究
短句来源
     By using the combination of laser induced fluorescence and crossed molecular beam meth- od, the processes of the state to state reaction dynamics of the reaction F + C_2F_5I→ IF + C_2F_5 were studied in detail in a wide wavelength region.
     本文用激光诱导荧光方法与分子束技术相结合,在较宽的波段范围内详细研究了F+C_2F_3I→IF+C_2F_5的态-态反应动力学过程。
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  fluorescence method
The effective segregation coefficients of W and Sr elements in SrWO4 single crystal are close to 1 by the X-ray fluorescence method.
      
The effect of dimethyl sulfoxide (DMSO) and diethyl sulfoxide (DESO) on micellization of sodium dodecyl sulfate in aqueous solutions is studied by fluorescence method using pyrene as a luminescence probe.
      
An Absorption-Fluorescence Method for Estimation of the Efficiency of Nonradiative Relaxation of Cyanine Dyes
      
The accuracy of the determination of elements by the proposed sorption-X-ray fluorescence method in river and sea water and in soil extract was verified by the added-found method.
      
The reaction between hydrogen iodide and ozone at 295 K has been investigated by the resonance fluorescence method applied to the detection of iodine atoms.
      
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  fluorometric method
A new, simple, sensitive and selective fluorometric method for the determination of nitrite has been developed.
      
A new highly sensitive, stable, and accurate fluorometric method for the determination of indium(III) has been established in cetyltrimethylammonium bromide(CTMAB) microemulsion media.
      
The automated fluorometric method was used for the determination of H2O2 .
      
Mitochondrial DNA (mtDNA) was assayed by a fluorometric method.
      
A fluorometric method for determining indomethacin in serum by reversed-phase high performance liquid chromatography has been developed.
      
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1.In this work the technique of perfusion of cerebral ventricular system inconscious rabbits was applied to the study of 5-hydroxytrytamine(5-HT)releaseand the effect of Midaflur on it.Midaflur(4mg/kg i.v)could not directly modify5-HT release in brain in conscious rabbits,but the increase in the 5-HT releasewas observed in rabbit struggling with the procedure of injection of Midaflur.Therefore,the behavior of the conscious rabbits must be considered in studyingthe effect of drugs on the release of neurotransmitters...

1.In this work the technique of perfusion of cerebral ventricular system inconscious rabbits was applied to the study of 5-hydroxytrytamine(5-HT)releaseand the effect of Midaflur on it.Midaflur(4mg/kg i.v)could not directly modify5-HT release in brain in conscious rabbits,but the increase in the 5-HT releasewas observed in rabbit struggling with the procedure of injection of Midaflur.Therefore,the behavior of the conscious rabbits must be considered in studyingthe effect of drugs on the release of neurotransmitters in brain by perfusion of thecerebral ventricular system.2.Dopamine content was determined by the spectrophotofluorometric me-thod.The experimental results demonstrated that no significant change in dopa-mine content in caudate nucleus as well as in hippocampus was observed duringthe muscle relaxing.

1.用~3H-5-羟色胺脑室灌流清醒动物的方法,测定了脑内5-羟色胺的释放。实验结果表明,给兔静注咪达氟(4mg/kg)后,因动物挣扎引起5-羟色胺释放加强,可能咪达氟不直接加强兔脑5-羟色胺的释放。因此,必须指出,在用清醒动物脑室灌流的方法研究药物对神经递质释放是否有影响时,必须考虑到清醒动物的活动状态。2.用荧光方法测定了多巴胺含量。实验结果表明,给兔静注眯达氟(4或8mg/kg)以后10分钟,颈部和前肢产生明显肌松时海马中多巴胺含量虽有增加的趋势,但无统计学意义,尾核中多巴胺含量基本不变。因此,可以认为咪达氟所引起的肌肉松弛可能与脑内多巴胺含量关系不大。

A convenient ultra-micromethod for determining free amino acid contents in caudatoputamen of mice was presented.The determination of γ-aminobutyric acid (GABA) and taurine contents was carried out with Dansyl chloride and microchromatography on polyamide thin layer and measured by the fluorescence method with some modification.It was found that the mixture solvent, alcohol: conc. ammonia water (9 : 1, v/v), is the suitable solvent for eluating Dans-taurine from the polyamide thin layer (made in China). Recoveries...

A convenient ultra-micromethod for determining free amino acid contents in caudatoputamen of mice was presented.The determination of γ-aminobutyric acid (GABA) and taurine contents was carried out with Dansyl chloride and microchromatography on polyamide thin layer and measured by the fluorescence method with some modification.It was found that the mixture solvent, alcohol: conc. ammonia water (9 : 1, v/v), is the suitable solvent for eluating Dans-taurine from the polyamide thin layer (made in China). Recoveries of GABA and taurine amount to 95-113% and 82-94% respectively as measured with this method and the sensitivity of which is about 10~(-12) M, approximate to that of liquid scintillation counting. It would be convenient and sensitive enough to determine 0.5 mg of caudatoputamen in mice and still applicable even less amount. And it was shown that the relation between emission of Dans-GABA and Dans-taurine and concentration of GABA and taurine are well linear, covering 0-60×10~(-12)M and 0-300×10~(-12)M respectively.With this available method the GABA and taurine contents in caudatoputamen of mice are determined to be 1.26±0.06 and 15.5±1.0 μM/g wet weight respectively.

本工作将DANS反应和微量薄膜层析技术与荧光测量加以修改后联用,并注意了组织样品的去蛋白和DANS-牛磺酸洗脱剂的改进,获得了满意的定量测定氨基酸的结果。GABA、牛磺酸的浓度(GABA:0—60×10~(-12)克分子;牛磺酸:0—300×10~(-12)克分子)与其相应的DANS衍生物的荧光强度成线性关系。回收率分别为95—113%和82—94%。灵敏度比原有荧光方法提高了近100倍(10~(-12)克分子数量级)。用此法测得小鼠尾壳核中的GABA和牛磺酸分别为1.26±0.06和15.5±1.0微克分子/克湿重,组织样品只需0.5毫克或更少些。这为测定微量神经组织中的自由氨基酸提供了一种简便的超微量方法.

The content of γ-aminobutyric acid(GABA),taurine,aspartic acid,glutamicacid,alanine and glycine in several parts of mouse brain at different ages was de-termined by using the available ultramicrodansyl method with polyamide TLC,modified in this laboratory.What was found was as follows:(1)In the caudatoputamen the“inhibitory”transmitter GABA content significantly increased with age.And the taurine contentdecreased while the content of the“excitatory”transmitter aspartic acid and glutamicacid significantly increased...

The content of γ-aminobutyric acid(GABA),taurine,aspartic acid,glutamicacid,alanine and glycine in several parts of mouse brain at different ages was de-termined by using the available ultramicrodansyl method with polyamide TLC,modified in this laboratory.What was found was as follows:(1)In the caudatoputamen the“inhibitory”transmitter GABA content significantly increased with age.And the taurine contentdecreased while the content of the“excitatory”transmitter aspartic acid and glutamicacid significantly increased during postnatal development up to adulthood.However,there was no change in content of these amino acids except in GABAcontent from adulthood to old age.(2)In the motor cortex the content of the“inhibitory”transmitter taurine and glycine decreased while the“excitatory”transmitter glutamic acid increased during postnatal development and the“in-hibitory”transmitter GABA continued to increase during ageing.(3)In thespinal cord the“inhibitory”transmitter GABA and taUrine decreased whilethe“excitatory”transmitter aspartic acid increased during postnatal development.(4)The ratio of total“inhibitory”,to“excitatory”transmitter amino acid contentin the caudatoputamen,motor,cortex as well as in the spinal cord significantlydecreased during postnatal development,and no significant change in the ratio du-ring ageing was noted.The ratio is about 1 in the adult,》1 in the newborn,and only in the spinal cord <1 in the old age.The highest ratio of“in-hibitory”to“excitatory”transmitter amino acids was noted in the caudatoputa-men,especially in newborn mice.It could be biochemically characterized by thefunction of the caudatoputamen during development.

用本实验室改进的超微量 DANS 反应-聚酰胺薄膜层析-荧光方法测定了新生、成年和老年小鼠尾壳核、皮层运动区和脊髓的递质氨基酸:GABA、牛磺酸、甘氨酸、谷氨酸、天冬氨酸和丙氨酸。实验结果表明,不同年龄小鼠中枢神经系统不同部位的“抑制性”和“兴奋性”递质氨基酸的含量不同:(1)尾壳核的“抑制性”递质 GABA 含量随年龄的增长而增加。在发育过程中“兴奋性”递质天冬氨酸、谷氨酸也显著升高,而“抑制性”递质牛磺酸含量却显著降低。老化时除 GABA 继续升高外,所测定的其他递质氨基酸皆无显著变化。(2)皮层运动区的“抑制性”递质牛磺酸和甘氨酸含量在发育过程中是降低的,而“兴奋性”递质谷氨酸显著升高。在老化时只有“抑制性”递质 GABA 继续升高。(3)脊髓的“抑制性”递质 GABA 和牛磺酸含量在发育过程中显著降低,而“兴奋性”递质天冬氨酸含量却显著升高。(4)由新生到成年,尾壳核、皮层运动区和脊髓的“抑制性”与“兴奋性”递质氨基酸总量的比值是降低的。成年小鼠的此比值近似于1,新生小鼠此比值(?)1。而老年小鼠的脊髓此比值<1。新生小鼠尾壳核的高比值可能在生物化学上反映了其在发育期的功能特点。

 
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