转基因白桦 transgenic birch(18)   transgenic birch      Analysis of Genetic Variation of Transgenic Birch      转基因白桦的遗传变异分析 短句来源      Stability of Integration and Expression of Foreign Gene in Transgenic Birch and Analysis of Genetic Variation      转基因白桦中外源基因整合表达稳定性与遗传变异分析 短句来源      The result proved an improved reverse PCR method applied to T-DNA flanking sequence analysis of the transgenic birch was feasibility and rationality.      证明改良的反向PCR方法运用到转基因白桦T-DNA旁侧序列的可行性和合理性。 短句来源      2. The condition of reverse primer PCR (RP-PCR) and direct primer PCR method in transgenic birch was conformed. The result of reverse primer PCR (RP-PCR) and direct primer PCR associating with Southern blotting could determine the copy number of T-DNA integrated into the host genome.      2.确定用反向引物PCR(RP-PCR)和正向引物PCR结合的方法研究转抗虫基因白桦外源基因T-DNA整合的拷贝数的条件,并结合Southern杂交确定外源基因T-DNA整合到宿主基因组上的拷贝数,对转基因白桦整合的拷贝数进行分类。 短句来源      This paper took the insect-resistant transgenic birch (Betula platyphylla) grown in greenhouse for four years as study materials. The genomic DNA and RNA of leaves were extracted and the integration and the expression characteristic of the extraneous genes in the insect-resistant transgenic birch were analysed by methods of multiplex PCR (MPCR), reverse primer PCR (RP-PCR), inverse PCR (IPCR), RT-PCR, and insect-feeding experiment of the transgenic plants respectively.      本文以温室四年生转抗虫基因白桦(Betula platyphylla)为研究材料,提取叶片的基因组DNA和RNA,运用多重PCR,反向引物PCR,反向PCR和RT-PCR等方法,并结合转基因白桦的喂虫实验,分别对转基因白桦外源基因的整合特性和表达特性进行分析,所得结果如下: 短句来源 更多          “转基因白桦”译为未确定词的双语例句      Gus gene and npt II was 100%.      5.在20株转基因白桦中gus、nptⅡ、bt三个基因均已整合,三个基因的共整合频率达100%。 短句来源      The resistances to Lymantria dispar for 39 transgene clones and the control of Betula platyphylla were determined. The result showed that the highest resistant clones with the mortality more than 75% accounted for 15.38%, the middle level resistant clones with mortality of 41%~75% accounted for 46.1%, the lower resistant clones with mortality of 15%~40% accounted for 21.51%, clones with mortality less than 15% accounted for 17.95%.      利用39个转基因白桦无性系和对照植株分别对舞毒蛾幼虫进行了抗虫性测定,结果死亡率75%以上的高抗无性系占15. 38%,死亡率在41% ~75%的中抗无性系占46. 15%,死亡率15% ~40%的低抗无性系占20. 51%,死亡率15%以下的无性系占17. 95%。 短句来源      Class Division of Resistance to Lymantria dispar for Transgene Betula platyphylla and Its Effect on Larvae Midgut      转基因白桦抗性等级划分及其对幼虫中肠的影响 短句来源      Resistance of Transgenic Betula platyphylla to the Defoliator Lymantria dispar      转基因白桦对舞毒蛾的抗性研究 短句来源      In this study 14 arbitrary primers were used to analyze genetic variation between transgenic plant population and control population.      同时用RAPD标记方法研究了转基因白桦在DNA水平的变异情况,结果显示在转基因苗群体中,14个引物共检测到90个位点,其中多态位点57个,占63.33%; 短句来源 更多          相似匹配句对      Analysis of Genetic Variation of Transgenic Birch      转基因白桦的遗传变异分析 短句来源      Resistance of Transgenic Betula platyphylla to the Defoliator Lymantria dispar      转基因白桦对舞毒蛾的抗性研究 短句来源      Genetically Modified Oil Crops      转基因油料作物 短句来源      GM FOOD Genetically Modified Food      转基因食品 短句来源      Research of Ectomycorrhiza of Betula Platyphylla Suk      白桦外生菌根的研究 短句来源 查询“转基因白桦”译词为用户自定义的双语例句     我想查看译文中含有：的双语例句 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。   transgenic birch This report demonstrates that the constitutive expression of this gene in transgenic birch lines increased the resistance of birch against the leaf spot fungus P. 例句来源       Evidence for ET insensitivity in the transgenic birch lines carrying Arabidopsis ET receptor gene with the dominant etr1-1 mutation. 例句来源                The paper reported for the first time that transgenic plants of Betula platyphylla had been obtained through mediation with Agrobacterium , in which the chimeric gene of spider insecticidal peptide gene and the C peptide sequence of Bt gene was used as purpose foreign gene for this transformation experiment. The effects of duration of co culture, AS and the type of explants on the Agrobacterium mediated transformation were studied. It showed that 2 d co culture had been suitable for bi...             应用农杆菌介导法首次向白桦 (Betula platy phyllaSuk .)基因组中导入抗虫基因 [蜘蛛杀虫肽与苏云金杆菌杀虫毒蛋白基因 (Bt基因 )C肽序列的嵌合基因 ]。转化结果表明 :共培养 2d的脱菌及防止腐烂的效果和转化率均高 ,液体共培养及液体除菌优于固体共培养及固体除菌 ;最佳外植体为大叶片 ;侵染 2个月之后产生抗性愈伤组织 ,转化率达到 2 2 %。卡那霉素抗性植株中 ,GUS阳性率为 4 3%。进行杀虫肽和nptⅡ基因的PCR(聚合酶链式反应 )均检测出目的带。转化植株目的基因的PCRSouthern(DNA印记杂交 )杂交呈阳性 ,证明获得了转基因植株。初步的喂虫试验表明转基因白桦具有一定的抗虫性 文摘来源          The occurrence of somaclonal variation in transgenic birch plants produced by infecting calli with Agrobacterium tumefaciens has been verified by cytological and RAPD analysis. The cytological analysis of transgenic plants showed that the frequency of non-diploid is 78.5%, and the non-transgenic control is 15.3%. The RAPD result indicated the transgenic plants' PI is 31.67.The comparison of data produced in previous analyses of transgenic poplar obtained by infecting calli with Agrobacterium tumefaciens ( P...             应用细胞学方法分析了由农杆菌介导法获得的转基因白桦的细胞学变异情况,结果表明转基因白桦的染色体变异频率为78.5％,远远高于非转基因白桦的变异频率(15.3％),且变异以非整倍体占多数。同时用RAPD标记方法研究了转基因白桦在DNA水平的变异情况,结果显示DNA多态性指数为31.67,并与其它转基因植物的变异情况作了比较研究。最后分析、讨论了产生变异的原因:(1)组织培养过程中产生突变;(2)外源基因的整合及重排时宿主基因组的插入位点及相邻基因转录表达的干扰;(3)应用抗生素和除草剂等筛选转基因植株时促进了转基因植株的变异程度。并提出减少转基因植物体细胞克隆变异的建议。 文摘来源          The resistances to Lymantria dispar for 39 transgene clones and the control of Betula platyphylla were determined. The result showed that the highest resistant clones with the mortality more than 75% accounted for 15.38%, the middle level resistant clones with mortality of 41%~75% accounted for 46.1%, the lower resistant clones with mortality of 15%~40% accounted for 21.51%, clones with mortality less than 15% accounted for 17.95%. For larvae of Lymantria dispar fed by leaves of transgene Betula platyphyll...             利用39个转基因白桦无性系和对照植株分别对舞毒蛾幼虫进行了抗虫性测定,结果死亡率75%以上的高抗无性系占15. 38%,死亡率在41% ~75%的中抗无性系占46. 15%,死亡率15% ~40%的低抗无性系占20. 51%,死亡率15%以下的无性系占17. 95%。通过转基因白桦叶片饲喂舞毒蛾幼虫,取食90h后中肠受到破坏,取食量开始明显减少; 144h取食量后受到严重影响,中肠内食物与对照相比明显减少,证明转基因白桦叶片中的毒素可以影响和破坏舞毒蛾幼虫中肠,降低其取食量,从而达到抑制害虫保护林木的目的。 文摘来源   << 更多相关文摘         相关查询: 白桦 转基因 转基因苜蓿 转基因白菜 抗病转基因 转基因安全性 转基因品系 转基因草鱼 同源转基因 转基因柞蚕 转基因算子 转基因小球藻  对查询结果不满意

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