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愈伤组织细胞
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  callus cells
     Identification of Gravity-related Esterases (grEST1 and grEST2) in Carrot Callus Cells
     胡萝卜愈伤组织细胞中与重力相关酯酶(grEST1和grEST2)的鉴别
短句来源
     The callus subcutured on MS + 0. 5 mg/L 2,4-D+0. 2 mg/L 6-BA grew well and formed green-thick callus. The callus cells were mostly diploid,a few sub-diploid and other ploidy cells.
     愈伤组织继代增殖的最佳培养基为MS+0.5mg/L 2,4-D +0.2 mg/L 6-BA,在其上继续生长,形成淡绿色、致密的愈伤组织,愈伤组织细胞主要为二倍体,也有少量的亚二倍体细胞和其它倍性细胞产生。
短句来源
     The Study of Human Interferon-alpha 2b Expressed by Ginseng Callus Cells
     人参愈伤组织细胞表达人干扰素α 2b的研究
短句来源
     Results:Stable integration of the hIFN-α2b gene in the Ginseng callus cells′ genome was confirmed by PCR analysis.
     结果:经PCR检测表明hIFN-α2b基因已成功整合到人参愈伤组织细胞基因组中。
短句来源
     The pBIFN was transformed into Agrobacterium tumefaciens strain LBA4404. Then hIFN-α2b gene was introduced into Ginseng callus cells via Agrobacterium-mediated transformation.
     用冻融法将pBIFN导入根瘤农杆菌LBA4404,经卡那霉素和利福霉素筛选后利用农杆菌介导的转化法将hIFN-α2基因导入人参愈伤组织细胞
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  callus cell
     The chromosome number and ploidy of callus cell and the root tip cell of the plant obtained are variable. Among the regenerated plant there were euploid cells (2n=10, 20, 30, 40, 50, etc) and several kinds of aneuploid cells (2n = 5-49)
     所得到的愈伤组织细胞和植株根尖细胞染色体数目和倍性是不稳定的,二者有相同的趋向,其中有整倍体的细胞(2n=10,20,30,40,50),也有各种非整倍体的细胞(2n=5—49)。
短句来源
     The callus cell derived from the rape mesophyll cell resembled parenchymacell of intact plant and there was also a central vacuole and a layer of cytoplasm withsome organelles.
     脱分化的愈伤组织细胞类似于薄壁细胞,中央也有一个大液泡,液泡周围有一层含有一些细胞器的细胞质。
短句来源
     Histocytological observation revealed that the callus cell was haploidy with, N=12. Differentiation of green buds occurred on the medium supplemented with 0 1 mg/L ABA, whereas no plantlet was developed, however.
     经细胞学观察证明:愈伤组织细胞确为单倍性的,染色体数目为n= 12,正常的体细胞染色体数目为2n= 24,并在含有ABA 的原诱导愈伤组织培养基上分化出绿色小芽
短句来源
     Analysis of free amino acid content shows that transformed tumor tissue has two to three times higher total free amino acid contents than normal callus cell line, and aspartic acid content in transformed tumor tissue is 18 to 37 times contents in callus cell line. There are obvious discrepancy between C58 transformed tumor tissue and T37 transformed tumor tissue,and T37 transformed tumor tissue has much higher contents of glutamic acid,arginine and prolinet than C58 transformed tumor tissue.
     游离氨基酸含量的分析表明,转化瘤组织的总游离氨基酸含量比正常愈伤组织细胞系高2-3倍,其中除转化瘤组织中的天门冬氨酸含量比正常愈伤组织细胞系高18-37倍,C58转化瘤组织和T37转化瘤组织也显示出明显差异,其中,T37转瘤组织中的谷氨酸,精氨酸和脯氨酸含量比C58转化瘤组织和正常愈伤组织细胞系高得多的.
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  “愈伤组织细胞”译为未确定词的双语例句
     B_5 medium with the addition of 2,4-D(5 mg·L~(-1)), 6-BA(0.5 mg·L~(-1)) and AC(1 g/L) gave optimum cell growth and the darkening in subculture also could be inhibited someway.
     在含有5mg·L-12,4 D5+0 5mg·L-16 BA+1g·L-1AC的B5培养基上,愈伤组织细胞增殖倍数最大,达到4 7,褐化也得到部分控制.
短句来源
     Five different ecotypic garlic cultivars were chosen to select cell lines with salttolerance on solid MS medium containing 0.1%,0.3%,0.7%,1.0%,1.5% or 2.0% NaCl via direct inoculation and gradual culture method.
     用含有0.1%、0.3%、0.7%、1.0%、1.5%、2.0%NaCl的MS培养基,采用直接接种和逐级培养法,对5个不同生态型的大蒜品种进行耐盐愈伤组织细胞系的选择研究.
短句来源
     Swell pieces of calli were observed under the microscope and it was revealed that the nonembroynic cells of the callus had changed to embroynic cells from which plant was developed through somatic embryogenesis on the MS culture medium supplemented with 2 mg/L 6 BA and 0 5 mg/L NAA.
     在 MS+6 - BA0 .1mg/ L+NAA0 .5mg/ L+CH50 0 mg/ L培养基上继代培养 ,再转入 MS+6 - BA2 mg/L +NAA 0 .5mg/ L的分化培养基上进行分化培养。 显微观察表明 ,在培养过程中愈伤组织细胞由非胚性细胞转变为胚性细胞 ,直至发育成体细胞胚胎和完整植株 ;
短句来源
     The cutting pieces of bulb of Fritillariae pallidiflorae were cultured on MS media supplemented with 2, 4-D, IAA, NAA or 2, 4-D+KT、 IaA+KT, NAA+KT. (2, 4-D, IAA, NAA 1mg/l, KT 0.1mg/l). Chromsome variations in calli and callus differentiation and chromosome ploidy of regenerated plants were studed.
     伊贝母鳞茎培养在附加2,4—D、IAA、NAA和2,4—D+KT、IAA+KT、NAA+KT的MS培养基上(2,4—D、IAA、NAA1毫克/升,KT0.1毫克/升),研究了愈伤组织细胞染色体的变异及愈伤组织的分化和再生植株的染色体倍性。
短句来源
     Callus as cultured in LS medium containing sugar or glucose or maltose or soluble starch with 1 % , 2% , 3%,4% concentration respectively , added with 2,4 - D 3mg/L, 6- BA 2. 5mg/L, NAA 0.4 mg/L, the effects of various kinds and concentrations of carbon on callus growth rate and special activity of agavain - SH were studied.
     以LS培养基,分别添加蔗糖、葡萄糖、麦芽糖和可溶性淀粉四种碳源,各1%、2%、3%和4%四种浓度,附加2,4—D 3mg/L,6—BA2.5mg/L,NAA 0.4mg/L,比较了不同种类及浓度的碳素营养对剑麻愈伤组织细胞生长和蛋白酶诱导的影响。
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  callus cells
Light and Nitrate Utilization by Soybean Callus Cells
      
We studied the role of light during exogenous assimilation of nitrate (the only source of nitrogen) by the callus cells of soybean (Glycine max).
      
The experimental results obtained were used to determine the possible causes of the heterogeneity of callus cells as regards their anoxia resistance.
      
In the SA-treated infected calli, the formation of necrotic lesions was observed in the zones of contact of the fungal mycelium with callus cells that limited pathogen growth.
      
Light and electron microscopic examinations revealed a large number of intracellular prismatic-shaped Ca oxalate crystals in both leaf and callus cells.
      
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  callus cell
The concentration of cytochrome P450 and ecdysone 20-monooxygenase activity in plants and callus cell culture of carpet bugleweed Ajuga reptans L.
      
It was demonstrated that the content of ecdysteroids in callus cell culture is higher than in the intact plant, with concurrent retention of a high ecdysone-20-monooxygenase activity.
      
All other cell lines contain a ribosome inhibitor, although only two callus cell lines show detectable amounts of immunoreactive proteins at the same Mr as dodecandrin.
      
Therefore, it appears that the ability of callus cell to differentiate into somatic embryos increases in the following order: octoploid >amp;lt; diploid >amp;lt; tetraploid.
      
The observed site of somatic embryo origin (SSEO) could originate from a superficial callus cell, possibly indicating a unicellular origin, or from epidermal and subepidermal callus cells, representing a multicellular origin.
      
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The present article deals with an investigation on the anther culture in vitro of tobacco and red pepper. The tobacco anthers were cultured in vitro on 4 different media.Best result was obtained with Nitsch H medium;Blaydes was ledd effective.The percentage of the embryoids formation increases with the addition of 10—20%(v/v)cocoanut milk.The embryoids may be induced with sucrose from 1—4%;and 3% is the optimum. The anthers of different developmental stages were cultured in vitro for com- parison.It is found...

The present article deals with an investigation on the anther culture in vitro of tobacco and red pepper. The tobacco anthers were cultured in vitro on 4 different media.Best result was obtained with Nitsch H medium;Blaydes was ledd effective.The percentage of the embryoids formation increases with the addition of 10—20%(v/v)cocoanut milk.The embryoids may be induced with sucrose from 1—4%;and 3% is the optimum. The anthers of different developmental stages were cultured in vitro for com- parison.It is found that when the anther with its pollen grains at the 1-nucleate stage,while the nucleus is situated on one side of the pollen,is the best material for haploid seedling induction. The embryoids were mainly formed in two ways:They were formed either from the development of equal division of the 1-nucleate pollen grain or from the vegetative nucleus of the unequal division of the 1-nucleate pollen grain.The generative nucleus may divide only once or several times but eventually the nucleus and its derivatives degenerate. The chromosome number of the root tip or shoot apex of the pollen plants is found to be n=24;the haploid nature of this plant was confirmed.Colchicine of different concentrations was used to double up the chromosomes of the haploid plantlets.In this way many diploids were obtained and among them a few were tetraploids.The production of the embryoids by different means was discussed. The red pepper anthers were cultured in vitro on NT and MS media,supplemented with different growth substances at the stage when its only nucleus is situated on one side of the pollen grain.The anthers gave rise to embryoids and calli.The develop- ment of the 1-nucleate pollen grain through“proembryo”to the formation of the seed- ling was observed.The development of the embryoids is similar to that of the zygotic embryo. The development of the embryoids from the pollen grains may cease to grow at any stage,hence a few of them may develop into seedlings. The connection between the multicellular“proembryo”and the pollen grain can be seen.The cells inside the pollen grain are large and stained lightly,while the cells of the embryoids outside the pollen grain are small,meristematic and closely arranged; some of them being in the initial stage of differentiation. The cells of the root tip and the callus have been examined with aceto-carmine squash method.The chromosome number was found to be n=12;therefore these plants and calli are haploids.

本文报道关于菸草和辣椒花药离体培养的研究结果。对于菸草花药离体培养曾进行四种培养基的对比试验,结果以 NH 效果最好,Blaydes 次之。10—20%(体积/体积)椰乳能提高花药成胚的百分比,对单倍体幼苗也有促进作用。蔗糖浓度从1—4%都能产生“胚状体”,以3%的蔗糖浓度产生“胚状体”的百分比最高,长出的幼苗也比较健壮。对花粉不同发育时期进行了比较试验,花药中花粉单核靠边时期进行培养的出苗率最高。“胚状体”的形成主要由单核花粉粒均等分裂或由营养核分裂两种方式形成“胚状体”。生殖核只进行几次分裂而最后逐渐退化消失。对菸草花粉植株进行根尖或茎尖制片,观察其染色体数目为24,证明是单倍体。用不同浓度的秋水仙碱对单倍体植株进行染色体加倍,得到许多二倍体后代,其中少数是四倍体。本文对花药离体培养和组织培养中产生“胚状体”问题进行了讨论。辣椒采用花粉单核靠边时期的花药,培养在 NT 及 MS 加有补充物质的培养基上,花药既产生“胚状体”又产生愈伤组织。观察到单核花粉最初分裂发育成多细胞“原胚”到最后形成幼苗的过程。“胚状体”的发育与合子胚的发育过程相似。在花粉发育成幼苗的各个发育时期都可能停止发育,长成幼苗的只是少数。...

本文报道关于菸草和辣椒花药离体培养的研究结果。对于菸草花药离体培养曾进行四种培养基的对比试验,结果以 NH 效果最好,Blaydes 次之。10—20%(体积/体积)椰乳能提高花药成胚的百分比,对单倍体幼苗也有促进作用。蔗糖浓度从1—4%都能产生“胚状体”,以3%的蔗糖浓度产生“胚状体”的百分比最高,长出的幼苗也比较健壮。对花粉不同发育时期进行了比较试验,花药中花粉单核靠边时期进行培养的出苗率最高。“胚状体”的形成主要由单核花粉粒均等分裂或由营养核分裂两种方式形成“胚状体”。生殖核只进行几次分裂而最后逐渐退化消失。对菸草花粉植株进行根尖或茎尖制片,观察其染色体数目为24,证明是单倍体。用不同浓度的秋水仙碱对单倍体植株进行染色体加倍,得到许多二倍体后代,其中少数是四倍体。本文对花药离体培养和组织培养中产生“胚状体”问题进行了讨论。辣椒采用花粉单核靠边时期的花药,培养在 NT 及 MS 加有补充物质的培养基上,花药既产生“胚状体”又产生愈伤组织。观察到单核花粉最初分裂发育成多细胞“原胚”到最后形成幼苗的过程。“胚状体”的发育与合子胚的发育过程相似。在花粉发育成幼苗的各个发育时期都可能停止发育,长成幼苗的只是少数。由单核花粉粒所形成的多细胞“原胚”可突出花粉壁外但仍与花粉粒相连,花粉粒内细胞大而染色淡,花粉壁外的多细胞“原胚”的细胞排列紧密,体积小而有分生能力,有的已开始分化。小苗的根尖和愈伤组织细胞,用醋酸洋红压片检查,其染色体数目为12,证明是单倍体。

Graft between tomato and potato is described. After they are grafted, the first nuclear movements toward the wound location occur in several tiers of cells adjacent to the contact layer. This property of cell nuclei is enriched by the supply of nutrients and energy, required for wound union. Subsequently callus is formed chiefly by cell division of both scion and stock next to the contact layer. The types of cell division are of mitosis and amitosis,but the latter is more fre-quenty observed among the dividing...

Graft between tomato and potato is described. After they are grafted, the first nuclear movements toward the wound location occur in several tiers of cells adjacent to the contact layer. This property of cell nuclei is enriched by the supply of nutrients and energy, required for wound union. Subsequently callus is formed chiefly by cell division of both scion and stock next to the contact layer. The types of cell division are of mitosis and amitosis,but the latter is more fre-quenty observed among the dividing cells. The abundant parenchymatous cells in the herbaceous stem of dicotyledonous plants have the ability to develop cal-lus except the cambial cells, hence in grafting them it should not be emphasi-zed that cambial cells of the two plants must be joined.

番茄和马铃薯嫁接后,首先在伤口附近出现核趋伤运动。细胞核的趋伤性促进了物质和能量的供应,对伤口的愈合甚为需要。其次是砧木和接穗二者贴近伤口的细胞分裂,产生愈伤组织。在草本茎中有发达的薄壁组织细胞,它们是除形成层外产生愈伤组织的细胞来源。愈伤组织形成后,沿伤口由薄壁细胞分化成木质部分子,将砧木和接穗的木质部连接起来,使维管束愈合。薄壁细胞变化成木质部分子的途径,在轴向是一曲线而不是一直线。

This paper deals with the optimum culture medium inducing callus from excised Collars, i. e. buds on the root tips and excised petiole segmenes of Angelica sinesis and with the effect of hormones and difference in temperature upon the organic differentiation from callus. Cytological observation demonstrated that the plant regeneration from callus were completed in two ways, the formations of embryoid and adventitious bud. The methods of thin-Layer chromatography and paper chromatography have been used to identify...

This paper deals with the optimum culture medium inducing callus from excised Collars, i. e. buds on the root tips and excised petiole segmenes of Angelica sinesis and with the effect of hormones and difference in temperature upon the organic differentiation from callus. Cytological observation demonstrated that the plant regeneration from callus were completed in two ways, the formations of embryoid and adventitious bud. The methods of thin-Layer chromatography and paper chromatography have been used to identify the ability of the cultures to synthesize essential oil and carbohydrate. The result shows that the cultures may produce the same essential oil and carbohydrate as the root of cultivated Angelica sinesis does.

本文报道了用当归离体根颈即根端的芽和叶柄切段诱导愈伤组织的适宜培养基及激素与温度对器官分化的效应。通过愈伤组织的细胞学观察,判断植株再生方式为胚状体和不定芽(苗)。还应用薄层层析、纸层析法,初步鉴定证明所获得的培养物具有合成与栽培当归根同样成分的挥发油和碳水化合物的能力,进一步证明了一些学者提出的“植物的离体细胞在人工培养下,具有药物生物合成的全能性”。

 
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