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愈伤组织细胞
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  callus cells
    Scanning Electron Microscopy of ODO Freeze Fracture on Embryogenic Callus Cells of Barley
    大麦胚性愈伤组织细胞ODO冷冻断裂面的扫描电镜观察
短句来源
    Identification of Gravity-related Esterases (grEST1 and grEST2) in Carrot Callus Cells
    胡萝卜愈伤组织细胞中与重力相关酯酶(grEST1和grEST2)的鉴别
短句来源
    The Study of Human Interferon-alpha 2b Expressed by Ginseng Callus Cells
    人参愈伤组织细胞表达人干扰素α 2b的研究
短句来源
    On the basis of identification of two gravity related esterases (grEST1 and grEST2) in carrot callus cells (Cai et al . 1998), we continued the study of the characteristics of these two esterases.
    在从胡萝卜愈伤组织细胞中分离出两个与重力作用相关的酯酶(grEST1 和grEST2)后( 蔡伟明等1998) ,对这两个酯酶的性质作了进一步研究。
短句来源
    The pBIFN was transformed into Agrobacterium tumefaciens strain LBA4404. Then hIFN-α2b gene was introduced into Ginseng callus cells via Agrobacterium-mediated transformation.
    用冻融法将pBIFN导入根瘤农杆菌LBA4404,经卡那霉素和利福霉素筛选后利用农杆菌介导的转化法将hIFN-α2基因导入人参愈伤组织细胞
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  callus cell
    The chromosome number and ploidy of callus cell and the root tip cell of the plant obtained are variable. Among the regenerated plant there were euploid cells (2n=10, 20, 30, 40, 50, etc) and several kinds of aneuploid cells (2n = 5-49)
    所得到的愈伤组织细胞和植株根尖细胞染色体数目和倍性是不稳定的,二者有相同的趋向,其中有整倍体的细胞(2n=10,20,30,40,50),也有各种非整倍体的细胞(2n=5—49)。
短句来源
    The callus cell derived from the rape mesophyll cell resembled parenchymacell of intact plant and there was also a central vacuole and a layer of cytoplasm withsome organelles.
    脱分化的愈伤组织细胞类似于薄壁细胞,中央也有一个大液泡,液泡周围有一层含有一些细胞器的细胞质。
短句来源
    Histocytological observation revealed that the callus cell was haploidy with, N=12. Differentiation of green buds occurred on the medium supplemented with 0 1 mg/L ABA, whereas no plantlet was developed, however.
    经细胞学观察证明:愈伤组织细胞确为单倍性的,染色体数目为n= 12,正常的体细胞染色体数目为2n= 24,并在含有ABA 的原诱导愈伤组织培养基上分化出绿色小芽
短句来源
  “愈伤组织细胞”译为未确定词的双语例句
    STUDY ON CYTOKININ BINDING PROTEIN FROM CELL OF BAIBAILI TOBACCO CALLUS
    白百利烟草愈伤组织细胞细胞分裂素结合蛋白的研究
短句来源
    The cutting pieces of bulb of Fritillariae pallidiflorae were cultured on MS media supplemented with 2, 4-D, IAA, NAA or 2, 4-D+KT、 IaA+KT, NAA+KT. (2, 4-D, IAA, NAA 1mg/l, KT 0.1mg/l). Chromsome variations in calli and callus differentiation and chromosome ploidy of regenerated plants were studed.
    伊贝母鳞茎培养在附加2,4—D、IAA、NAA和2,4—D+KT、IAA+KT、NAA+KT的MS培养基上(2,4—D、IAA、NAA1毫克/升,KT0.1毫克/升),研究了愈伤组织细胞染色体的变异及愈伤组织的分化和再生植株的染色体倍性。
短句来源
    The neomycin phosphotransferaseⅡ(NPTⅡ)gene of the plasmid pLGVneo2103 was transferred into the tissue cells of tobacco leaves and the calli of corn and soybean by using the condenser discharge mode electroporation system, which was designed and constructed by our laboratory.
    用自制的电容放电式电激系统,将质粒pLGVneo2103上的NPTⅡ基因直接导入到烟草叶片组织细胞和玉米、大豆的愈伤组织细胞中。
短句来源
    Cytokinin binding proteins (CB-protein) which moleculer weight arc about 4400±100 dalton from cell of Baibaili tobacco callus was purified by affinity chromatography on benzylaminopurin-Knked sephrosc 4B column.
    利用BA-Sepharose 4B亲和层析技术丛白百利烟草(Nicotiana tabacum Baibaili)愈伤组织细胞分离提纯了分子量为4400±100道尔顿的细胞分裂素结合蛋白(CB-蛋白)。
短句来源
    Swell pieces of calli were observed under the microscope and it was revealed that the nonembroynic cells of the callus had changed to embroynic cells from which plant was developed through somatic embryogenesis on the MS culture medium supplemented with 2 mg/L 6 BA and 0 5 mg/L NAA.
    在 MS+6 - BA0 .1mg/ L+NAA0 .5mg/ L+CH50 0 mg/ L培养基上继代培养 ,再转入 MS+6 - BA2 mg/L +NAA 0 .5mg/ L的分化培养基上进行分化培养。 显微观察表明 ,在培养过程中愈伤组织细胞由非胚性细胞转变为胚性细胞 ,直至发育成体细胞胚胎和完整植株 ;
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  callus cells
Light and Nitrate Utilization by Soybean Callus Cells
      
We studied the role of light during exogenous assimilation of nitrate (the only source of nitrogen) by the callus cells of soybean (Glycine max).
      
The experimental results obtained were used to determine the possible causes of the heterogeneity of callus cells as regards their anoxia resistance.
      
In the SA-treated infected calli, the formation of necrotic lesions was observed in the zones of contact of the fungal mycelium with callus cells that limited pathogen growth.
      
Light and electron microscopic examinations revealed a large number of intracellular prismatic-shaped Ca oxalate crystals in both leaf and callus cells.
      
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  callus cell
The concentration of cytochrome P450 and ecdysone 20-monooxygenase activity in plants and callus cell culture of carpet bugleweed Ajuga reptans L.
      
It was demonstrated that the content of ecdysteroids in callus cell culture is higher than in the intact plant, with concurrent retention of a high ecdysone-20-monooxygenase activity.
      
All other cell lines contain a ribosome inhibitor, although only two callus cell lines show detectable amounts of immunoreactive proteins at the same Mr as dodecandrin.
      
Therefore, it appears that the ability of callus cell to differentiate into somatic embryos increases in the following order: octoploid >amp;lt; diploid >amp;lt; tetraploid.
      
The observed site of somatic embryo origin (SSEO) could originate from a superficial callus cell, possibly indicating a unicellular origin, or from epidermal and subepidermal callus cells, representing a multicellular origin.
      
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The present article deals with an investigation on the anther culture in vitro of tobacco and red pepper. The tobacco anthers were cultured in vitro on 4 different media.Best result was obtained with Nitsch H medium;Blaydes was ledd effective.The percentage of the embryoids formation increases with the addition of 10—20%(v/v)cocoanut milk.The embryoids may be induced with sucrose from 1—4%;and 3% is the optimum. The anthers of different developmental stages were cultured in vitro for com- parison.It is found...

The present article deals with an investigation on the anther culture in vitro of tobacco and red pepper. The tobacco anthers were cultured in vitro on 4 different media.Best result was obtained with Nitsch H medium;Blaydes was ledd effective.The percentage of the embryoids formation increases with the addition of 10—20%(v/v)cocoanut milk.The embryoids may be induced with sucrose from 1—4%;and 3% is the optimum. The anthers of different developmental stages were cultured in vitro for com- parison.It is found that when the anther with its pollen grains at the 1-nucleate stage,while the nucleus is situated on one side of the pollen,is the best material for haploid seedling induction. The embryoids were mainly formed in two ways:They were formed either from the development of equal division of the 1-nucleate pollen grain or from the vegetative nucleus of the unequal division of the 1-nucleate pollen grain.The generative nucleus may divide only once or several times but eventually the nucleus and its derivatives degenerate. The chromosome number of the root tip or shoot apex of the pollen plants is found to be n=24;the haploid nature of this plant was confirmed.Colchicine of different concentrations was used to double up the chromosomes of the haploid plantlets.In this way many diploids were obtained and among them a few were tetraploids.The production of the embryoids by different means was discussed. The red pepper anthers were cultured in vitro on NT and MS media,supplemented with different growth substances at the stage when its only nucleus is situated on one side of the pollen grain.The anthers gave rise to embryoids and calli.The develop- ment of the 1-nucleate pollen grain through“proembryo”to the formation of the seed- ling was observed.The development of the embryoids is similar to that of the zygotic embryo. The development of the embryoids from the pollen grains may cease to grow at any stage,hence a few of them may develop into seedlings. The connection between the multicellular“proembryo”and the pollen grain can be seen.The cells inside the pollen grain are large and stained lightly,while the cells of the embryoids outside the pollen grain are small,meristematic and closely arranged; some of them being in the initial stage of differentiation. The cells of the root tip and the callus have been examined with aceto-carmine squash method.The chromosome number was found to be n=12;therefore these plants and calli are haploids.

本文报道关于菸草和辣椒花药离体培养的研究结果。对于菸草花药离体培养曾进行四种培养基的对比试验,结果以 NH 效果最好,Blaydes 次之。10—20%(体积/体积)椰乳能提高花药成胚的百分比,对单倍体幼苗也有促进作用。蔗糖浓度从1—4%都能产生“胚状体”,以3%的蔗糖浓度产生“胚状体”的百分比最高,长出的幼苗也比较健壮。对花粉不同发育时期进行了比较试验,花药中花粉单核靠边时期进行培养的出苗率最高。“胚状体”的形成主要由单核花粉粒均等分裂或由营养核分裂两种方式形成“胚状体”。生殖核只进行几次分裂而最后逐渐退化消失。对菸草花粉植株进行根尖或茎尖制片,观察其染色体数目为24,证明是单倍体。用不同浓度的秋水仙碱对单倍体植株进行染色体加倍,得到许多二倍体后代,其中少数是四倍体。本文对花药离体培养和组织培养中产生“胚状体”问题进行了讨论。辣椒采用花粉单核靠边时期的花药,培养在 NT 及 MS 加有补充物质的培养基上,花药既产生“胚状体”又产生愈伤组织。观察到单核花粉最初分裂发育成多细胞“原胚”到最后形成幼苗的过程。“胚状体”的发育与合子胚的发育过程相似。在花粉发育成幼苗的各个发育时期都可能停止发育,长成幼苗的只是少数。...

本文报道关于菸草和辣椒花药离体培养的研究结果。对于菸草花药离体培养曾进行四种培养基的对比试验,结果以 NH 效果最好,Blaydes 次之。10—20%(体积/体积)椰乳能提高花药成胚的百分比,对单倍体幼苗也有促进作用。蔗糖浓度从1—4%都能产生“胚状体”,以3%的蔗糖浓度产生“胚状体”的百分比最高,长出的幼苗也比较健壮。对花粉不同发育时期进行了比较试验,花药中花粉单核靠边时期进行培养的出苗率最高。“胚状体”的形成主要由单核花粉粒均等分裂或由营养核分裂两种方式形成“胚状体”。生殖核只进行几次分裂而最后逐渐退化消失。对菸草花粉植株进行根尖或茎尖制片,观察其染色体数目为24,证明是单倍体。用不同浓度的秋水仙碱对单倍体植株进行染色体加倍,得到许多二倍体后代,其中少数是四倍体。本文对花药离体培养和组织培养中产生“胚状体”问题进行了讨论。辣椒采用花粉单核靠边时期的花药,培养在 NT 及 MS 加有补充物质的培养基上,花药既产生“胚状体”又产生愈伤组织。观察到单核花粉最初分裂发育成多细胞“原胚”到最后形成幼苗的过程。“胚状体”的发育与合子胚的发育过程相似。在花粉发育成幼苗的各个发育时期都可能停止发育,长成幼苗的只是少数。由单核花粉粒所形成的多细胞“原胚”可突出花粉壁外但仍与花粉粒相连,花粉粒内细胞大而染色淡,花粉壁外的多细胞“原胚”的细胞排列紧密,体积小而有分生能力,有的已开始分化。小苗的根尖和愈伤组织细胞,用醋酸洋红压片检查,其染色体数目为12,证明是单倍体。

Graft between tomato and potato is described. After they are grafted, the first nuclear movements toward the wound location occur in several tiers of cells adjacent to the contact layer. This property of cell nuclei is enriched by the supply of nutrients and energy, required for wound union. Subsequently callus is formed chiefly by cell division of both scion and stock next to the contact layer. The types of cell division are of mitosis and amitosis,but the latter is more fre-quenty observed among the dividing...

Graft between tomato and potato is described. After they are grafted, the first nuclear movements toward the wound location occur in several tiers of cells adjacent to the contact layer. This property of cell nuclei is enriched by the supply of nutrients and energy, required for wound union. Subsequently callus is formed chiefly by cell division of both scion and stock next to the contact layer. The types of cell division are of mitosis and amitosis,but the latter is more fre-quenty observed among the dividing cells. The abundant parenchymatous cells in the herbaceous stem of dicotyledonous plants have the ability to develop cal-lus except the cambial cells, hence in grafting them it should not be emphasi-zed that cambial cells of the two plants must be joined.

番茄和马铃薯嫁接后,首先在伤口附近出现核趋伤运动。细胞核的趋伤性促进了物质和能量的供应,对伤口的愈合甚为需要。其次是砧木和接穗二者贴近伤口的细胞分裂,产生愈伤组织。在草本茎中有发达的薄壁组织细胞,它们是除形成层外产生愈伤组织的细胞来源。愈伤组织形成后,沿伤口由薄壁细胞分化成木质部分子,将砧木和接穗的木质部连接起来,使维管束愈合。薄壁细胞变化成木质部分子的途径,在轴向是一曲线而不是一直线。

The effects of auxin and kinetin on the microfibril arrangement of the primary cell wall of callus cells of Abutilon avicennae Gaertn.have been studied by scanning as well as trans-mission electron microscope at various intervals following hormonal administration.The callus was induced from the hypocotyl of A.avicennae and cul-tured in vitro.Prior to the experi-mentation,the induced callus cells were first cultured in diluted MS basal me-dium for the purpose to reduce the residual hormone of the cells.Three...

The effects of auxin and kinetin on the microfibril arrangement of the primary cell wall of callus cells of Abutilon avicennae Gaertn.have been studied by scanning as well as trans-mission electron microscope at various intervals following hormonal administration.The callus was induced from the hypocotyl of A.avicennae and cul-tured in vitro.Prior to the experi-mentation,the induced callus cells were first cultured in diluted MS basal me-dium for the purpose to reduce the residual hormone of the cells.Three days later they were treated separa-tely with IAA and kinetin.The obser-vations were made at 24,48 and 72 hours following hormonal treat-ments. The scanning electron micros-copic observation showed that the fibrils of the callus cell wall when cultured in medium without plant hormone,were short and arranged randomly or sometimes parallel in appearance.Projections were noted on the surface of the cell wall.Fol-lowing the treatment with IAA at a dose of 2 ppm for 24 hours,the struc-ture change beme apparent.The short randomly distributed fibrils grew longer and thicker than those of the controls.At 48 or 72 hours,the elongated fibrils slipped over each other forming a loose network.On the outer surface of the wall,there were many projections.Treatment of the cal-lus cells for 24 hours with kinetin at 10 ppm,the fibrils appeared coarse and interwove with one another.At 48 hours the fibrils exhibited a slightly ordered orientation,some with incrusted patches or scalelike materials disposed in the interfibrillar spaces.During this period,some of the cells started to elongate and the fibrils began to arrange themselves in bun-dles,showing a pattern of deposition at right angle to the growing axis of the cell.As the treatment con-tinued,the bundled fibrils became tightened in ring or spiral form.Fi-nally,at 72 hours,the entire cell dif-ferentiated into a tracheary element with spiral thickening. The ultrastructural change of the cell wall microfibrils at the undif-ferentiated stage was also noted under hormonal treatment. The results obtained in this experiment indicate that the orien-tation of the fibrils of the callus cell wall could be subjected to change under certain concentration of phy-tohormones.Kinetin at a dose of 10 ppm was shown to be able to induce callus cell differentiation and to form tracheary elements by changing the orientation of fibrils in the cell wall of A.avicennae.It is,therefore,rea-sonable to assume that the cellular differentiation of plant cell is probably regulated by the orientation of the fibrillar deposition.

本文报道了浓度为2ppm的生长素和10ppm的激动素对苘麻愈伤组织细胞初生壁上微丝排列的影响。经激素处理后,分别在24、48和72小时用扫描电镜和透射电镜观察。结果发现:经生长素处理24小时的细胞,壁上纤丝较对照的长,相互交叉;48和72小时后纤丝增长加多,形成一松散网状结构。经激动素处理的细胞,壁上纤丝较粗,48小时后部分细胞出现有方向性地成束状排列,同时细胞开始延长,继而纤丝密集成环状,及至72小时,这些细胞分化发育成为环纹或螺纹导管细胞。在透射电镜下也观察到激素处理后在未分化的细胞中壁上微纤丝排列也发生变化。本实验结果表明,苘麻愈伤组织细胞壁上微丝的排列方向可以被某一定浓度的植物激素所改变。浓度为10ppm的激动素可诱使苘麻愈伤组织细胞壁微丝沉积方位的改变,从而导致细胞分化成导管细胞。因此,有理由认为,植物细胞的分化可能与植物激素控制着壁上微丝沉积的方位也有关。

 
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