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   愈伤组织细胞 在 农业基础科学 分类中 的翻译结果: 查询用时:0.018秒
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愈伤组织细胞
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    -20~-40℃ was the major temperature scope of the callus cellinjury;
    愈伤组织细胞伤害的主要温度区域为-20~-40℃;
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  callus cell
The concentration of cytochrome P450 and ecdysone 20-monooxygenase activity in plants and callus cell culture of carpet bugleweed Ajuga reptans L.
      
It was demonstrated that the content of ecdysteroids in callus cell culture is higher than in the intact plant, with concurrent retention of a high ecdysone-20-monooxygenase activity.
      
All other cell lines contain a ribosome inhibitor, although only two callus cell lines show detectable amounts of immunoreactive proteins at the same Mr as dodecandrin.
      
Therefore, it appears that the ability of callus cell to differentiate into somatic embryos increases in the following order: octoploid >amp;lt; diploid >amp;lt; tetraploid.
      
The observed site of somatic embryo origin (SSEO) could originate from a superficial callus cell, possibly indicating a unicellular origin, or from epidermal and subepidermal callus cells, representing a multicellular origin.
      
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Being as cryoprotectants,5%DMSO- 10%glucuse or 5%DMSO- 10%sucrose could greatly reduce the injury of Actinidia chinensis callus cells. The in-jury of callus cells was minimal when freezed at the rate of- 1℃/min.to-60~-70℃。 The survival rate of the callus cells was maximal when rewarmed at the rate ofmore than 160℃/min. -20~-40℃ was the major temperature scope of the callus cellinjury;-60~- 75℃was the security temperature scope when callus cells were im-mersed in liquid nitrogen;-60℃ and-75℃ were the upper and...

Being as cryoprotectants,5%DMSO- 10%glucuse or 5%DMSO- 10%sucrose could greatly reduce the injury of Actinidia chinensis callus cells. The in-jury of callus cells was minimal when freezed at the rate of- 1℃/min.to-60~-70℃。 The survival rate of the callus cells was maximal when rewarmed at the rate ofmore than 160℃/min. -20~-40℃ was the major temperature scope of the callus cellinjury;-60~- 75℃was the security temperature scope when callus cells were im-mersed in liquid nitrogen;-60℃ and-75℃ were the upper and lower limits of criticaltemperatures respectively.

用5%二甲基亚砜+10%葡萄糖或5%二甲基亚砜+10%蔗糖作为冰冻保护剂,能有效减轻中华猕猴桃愈伤组织细胞的超低温伤害。以-1℃/min的速率降温至-60~-75℃时,对愈伤组织细胞的伤害作用最小;以大于160℃/min速率快速化冻,愈伤组织细胞的相对存活率最高。愈伤组织细胞伤害的主要温度区域为-20~-40℃;-60~-75℃为愈伤组织细胞投入液氮时的“安全温度区”,-60℃和-75℃分别为“临界上限温度”和“临界下限温度”。

Ti

利用农杆菌介导将带有新霉素磷酸转移酶(NPT-Ⅱ)基因和延缓衰老基因的Ti质粒导入番茄的愈伤组织细胞内,从而培养出能使番茄的浆果保存时间延长的植株。

 
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