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   前列腺癌细胞pc-3 的翻译结果: 查询用时:0.553秒
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前列腺癌细胞pc-
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  prostate cancer cell pc-3
     Effect of human prostate cancer cell PC-3 on the proliferation and alkaline phosphatase activity of mice osteoblast-like cell MC3T3-E1 under 1 G and simulated microgravity
     1G和模拟微重力下前列腺癌细胞PC-3对小鼠成骨样细胞MC3T3-E1增殖和活性的影响
短句来源
     Effect of inhibition of survivin expression on the apoptosis of prostate cancer cell pc-3
     抑制survivin表达对前列腺癌细胞pc-3凋亡的影响
短句来源
     Studies on the Synthesis of Polyoxometalates Containing Sulfanilamide and Their Inhibition to Prostate Cancer Cell PC-3M
     含有磺胺的多金属氧酸盐的合成及抑制前列腺癌细胞PC-3M作用的研究
短句来源
     [Results] Interruption of the survivin expression of prostate cancer cell pc-3 could induce the apoptosis of pc-3 cells. The mechanism might be that the apoptosis pathway of caspase-3 was activated.
     [结果]阻断survivin在前列腺癌细胞pc-3中的表达后,能够引起 pc-3细胞的凋亡,其机制可能是由于活化了凋亡通路的caspase-3。
短句来源
     [Methods] Survivin expression on prostate cancer cell pc-3 was interrupted by RNA interference (RNAi) technigue and the apoptosis ratio of prostate cancer cell PC-3 was determined by flow cytometer.
     [方法]运用RNA干扰技术阻断survivin在前列腺癌细胞pc-3中的表达,采用流式细胞计数等方法检测survivin 被阻断后,对前列腺癌细胞pc-3凋亡的影响。
短句来源
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  prostate cancer cell line pc-3
     This study was to explore the effects of Omi/HtrA2 on PED/ PEA-15 expression and apoptosis of prostate cancer cell line PC-3. METHODS: Omi/HtrA2 expression and specific siRNA vectors were constructed and transiently transfected into PC-3 cells.
     本研究旨在探讨Omi/HtrA2对PED/PEA-15表达和前列腺癌细胞PC-3凋亡的影响。
短句来源
     Experimental Study of the Growth Inhibition in Vitro on Human Prostate Cancer Cell Line PC-3M by TRAIL
     TRAIL抑制人前列腺癌细胞PC-3M体外生长的实验研究
短句来源
     AIM: To observe the effect of E2F decoy DNA on proliferation and apoptosis of androgen-independent prostate cancer cell line PC-3M.
     目的:为观察转录因子E2F陷阱DNA对雄激素非依赖性前列腺癌细胞PC-3M增殖和凋亡的影响。
短句来源
     Inhibition of Survivin Gene Expression in Prostate Cancer Cell Line PC-3 by Vector-based RNAi
     RNAi抑制前列腺癌细胞PC-3中survivin基因表达的实验研究
短句来源
     Objective: To observe effect of E2F decoy DNA on the proliferation of androgen-independent prostate cancer cell line PC-3M.
     目的观察转录因子E2F陷阱DNA策略对雄激素非依赖性前列腺癌细胞PC-3M增殖的影响。
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  “前列腺癌细胞pc-3”译为未确定词的双语例句
     VP16 INDUCED APOPTOSIS IN PC3 CELLS
     VP-16诱导前列腺癌细胞PC-3凋亡的研究
短句来源
     Inhibition effects of the PC-3M cells on expression of exogenous p21 gene
     前列腺癌细胞PC-3M对外源基因pPSA-P21表达的抑制作用
短句来源
     Inhibitory effects of poly I∶C on growth of PC-3M cell lines
     Poly I∶C对人前列腺癌细胞PC-3M的生长抑制作用
短句来源
     Conclusion: The eukaryotic expression vector pcDNA3.1-NKX3.1 is successfully constructed and effectually expressed in PC-3 and LNCaP cells.
     结论:成功构建了真核表达载体pcDNA3.1-NKX3.1,转染前列腺癌细胞PC-3和LNCaP后能有效表达。
短句来源
     The virus obtained from transfected PA317 cells was transfected respectively into PC-3m,LNCaP,MCF7,MRC5 cells. The expression of CXCR4 mRNA was detected by RT-PCR.
     转染PA317病毒包装细胞,收获病毒上清分别转染前列腺癌细胞PC-3m、LNCaP及人胚肺成纤维细胞MRC5和人乳腺癌细胞MCF7,RT-PCR检测CXCR4mRNA表达。
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  prostate cancer cell line pc-3
The CD profiles of cultured epithelial cell strains derived from normal compared with malignant tissues were notably similar to each other and to that of the prostate cancer cell line PC-3.
      
In order to establish an animal model of bone metastasis, cells from a human prostate cancer cell line (PC-3) were injected into the tail veins of athymic nude mice while the inferior vena cava was occluded.
      
Garlic Compound, Diallyl Disulfide Induces Cell Cycle Arrest in Prostate Cancer Cell Line PC-3
      
All the compounds were evaluated against the prostate cancer cell line PC-3.
      
All in vitro and in vivo experiments were conducted using the human prostate cancer cell line PC-3.
      
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In order to study the efficiency of the antibody directed enzyme prodrug therapy (ADEPT),the effect of MTX α peptides on growth and apoptosis of the prostatic cancer cells was studied.Methotrexate a Phenylalanine (MTX α Phe) and Methotrexate a arginine (MTX α Arg) were used as prodrugs synthesised by solid phase methods.The cytotoxin and cell cycle of prostatic cancer cells,PC 3 and PC 3m were determined.MTX α Phe was hydrolyzed into MTX by carboxypeptidase (CPA) using HPLC and Mass Spectrograph.The...

In order to study the efficiency of the antibody directed enzyme prodrug therapy (ADEPT),the effect of MTX α peptides on growth and apoptosis of the prostatic cancer cells was studied.Methotrexate a Phenylalanine (MTX α Phe) and Methotrexate a arginine (MTX α Arg) were used as prodrugs synthesised by solid phase methods.The cytotoxin and cell cycle of prostatic cancer cells,PC 3 and PC 3m were determined.MTX α Phe was hydrolyzed into MTX by carboxypeptidase (CPA) using HPLC and Mass Spectrograph.The cytotoxin effect of the products enzymed by CPA was 100 times higher than that of the prodrugs with no obvious cytotoxin on the cells in vitro and former showed an obvious inhibition effects on the cells.Their cell cycle was determined and S phase fraction was lower than the control group,G 0/G 1 fraction was higher and the perliferation index obviously decreased resulting in apoptosis of the cancer cells.MTX α Phe would be considered an excellent prodrug.

研究前列腺癌的导向酶前体药物疗法(ADEPT),在体外观察前体药物甲氨喋呤α肽对前列腺癌细胞生长、凋亡的影响。用固相合成法合成了前体药物甲氨喋呤α苯丙氨酸(MTXαPhe)和甲氨喋呤α精氨酸(MTXαArg),并对前列腺癌细胞PC3、PC3m进行了细胞毒性及周期动力学分析。用高效液相色谱及质谱分析,MTXαPhe可被羧肽酶A(CPA)水解并且完全释放出MTX。经体外对前列腺癌细胞的毒性试验,前体药物基本上对细胞无毒,经CPA水解后其活性MTX细胞毒性大于前体药物100倍以上。对前列腺癌细胞具有显著抑制作用,S期比率明显低于对照组,G0/G1期比率高于对照组,细胞增殖指数明显减低,细胞并发生凋亡。认为MTXαPhe是一较理想的前体药物。

AIM: To study the efficiency 0f the antibody di-rected enzyme-pr0drug therapy(ADEPT),the effect 0f MTX-a-peptides on the pr0state cancer was studied in vivo and invitro. METHODS: The cell-toxic effect and cell cycle analysisof Methotrexate -a- Phenylalanine (MTX-α-Phe ) andMethotrexate-α-Arginine (MTX-α-Arg ) on the cells 0fprostate cancer, PC-3 and PC-3m were determined. Theinhibition of the growth 0f the tumor of the former drugs innude mouse was observed. RESULTS: The prodrugs almosthad not the toxic effect...

AIM: To study the efficiency 0f the antibody di-rected enzyme-pr0drug therapy(ADEPT),the effect 0f MTX-a-peptides on the pr0state cancer was studied in vivo and invitro. METHODS: The cell-toxic effect and cell cycle analysisof Methotrexate -a- Phenylalanine (MTX-α-Phe ) andMethotrexate-α-Arginine (MTX-α-Arg ) on the cells 0fprostate cancer, PC-3 and PC-3m were determined. Theinhibition of the growth 0f the tumor of the former drugs innude mouse was observed. RESULTS: The prodrugs almosthad not the toxic effect on the test cell in vitro. MTX-α-Phewas hydrolyzed into MTX by Carboxypeptidase (CP-A). Thetoxin effect of the products was about 1OOO times higher thanthat of the prodrugs and the former showed an obvious inhibi-tion effects on the prostate cancer cells. Their cell-cycle wasdetermined and showed that the growth of the cell was inhib-ited in the S-phase. The inhibition on the tumor growth ofprostate cancer of the animal model was obviously in the ther-apy of the ADEPT with the A-MTX-α-Phe combined with theCP-A. The living condition was better than other groups.CONCLUSION: The MTX-α-Phe and the CP-A was an idealADEPT.

目的:研究前列腺癌的导向酶-前体药物疗法(ADEPT),在体内外观察前体药物甲氨喋呤-α-肽对前列腺癌的作用.方法:用前体药物甲氨喋呤-α-苯丙氨酸(MTX-α-Phe)和甲氨喋呤-α-精氨酸(MTX-α-Arg)对前列腺癌细胞PC-3,PC-3m进行了细胞毒性及细胞动力学分析,并对前列腺癌荷瘤裸鼠模型行肿瘤生长抑制的观察.结果:体外细胞毒性试验,前体药物对任何细胞均无毒,但MTX-α-Phe经羧肽酶-A(CP-A)水解后,其细胞毒性大于前体药物约1000倍,对前列腺癌细胞有显著抑制作用,细胞动力学分析肿瘤细胞生长明显受阻于S期.动物模型体内试验,经抗人精浆蛋白单克隆抗体导向的羧肽酶-A-MTX-α-PheADEPT治疗组,肿瘤生长明显抑制,动物生活质量优于单用MTX组.结论:MTX-α-Phe+CP-A是一理想的前列腺癌的ADEPT对.

Human prostate secretory protein of 94 amino acids (PSP94) cDNA including signal and mature peptide has been successfully obtained by reverse transcription polymerase chain reaction (RT PCR) from human hypertrophic prostate mRNA.Sequence analysis indicated that the sequence of human PSP94 cDNA was the same as that of human natural PSP cDNA.The hPSP94 cDNA and human TNFα mutant (TNF Δ) gene were fused by an artificial linker SAPGTP.The chimeric gene coding for 5′PSP SAPGTP TNF Δ fusion protein under control...

Human prostate secretory protein of 94 amino acids (PSP94) cDNA including signal and mature peptide has been successfully obtained by reverse transcription polymerase chain reaction (RT PCR) from human hypertrophic prostate mRNA.Sequence analysis indicated that the sequence of human PSP94 cDNA was the same as that of human natural PSP cDNA.The hPSP94 cDNA and human TNFα mutant (TNF Δ) gene were fused by an artificial linker SAPGTP.The chimeric gene coding for 5′PSP SAPGTP TNF Δ fusion protein under control of the phage P RP L promoter was constructed.The expression was assayed by SDS PAGE,which showed that the amount of the expressed the protein was about 35% of total bacterial proteins.Western blot indicated that the molecular weight of 5′PSP SAPGTP TNF Δ was in accordance with the estimated value of 31 kD.Through biological activity analysis,the fusion protein has cytotoxicity activity of L929 and PC 3 cell.

利用RT-PCR从人肥大前列腺组织钓取94个氨基酸的人前列腺分泌蛋白(PSP94)全长cDNA,序列分析结果与文献报道的完全一致.将PSP94成熟肽与人TNFα衍生物(TNFΔ)通过Linker-SAPGTP在基因水平上融合成5′PSP94-TNFΔ,融合基因DNA序列分析结果与设计的相符合.5′PSP94-TNFΔ在大肠杆菌中表达产物分子量约为31kD,表达量约占菌体总蛋白量的35%.以L929细胞和人前列腺癌细胞株PC-3为靶细胞进行细胞毒分析结果表明,5′PSP94-TNFΔ融合蛋白既具有TNF的细胞毒活性,又具有对前列腺癌细胞PC-3的杀伤作用

 
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