The studies on the fluorescence characteristic of the interaction between DNA and the complex of benzoic acid nitrogen mustard with Eu(Ⅲ)(Eu(BANM)_3·H_2O)in Tris-HCl medium (pH?7.2) suggested that the complex could be used as a fluorescence reagent for the determination of DNA.
In this paper,a review on achievement and progress of inorganic analysis and fluorometric analysis of China in 1984～1996 has been presented There are four parts in this paper:①Advances of new organic chromogenic and fluorometric reagents and its new reaction system;
Three new fluorescent reagents: 5-(4'-chloro-2'-carboxylphenylazo) rhodanine (CRACP), 5-(4'-bromo-2'-carboxylphenylazo) rhodanine (BRACP) and 5-(4'-iodo-2'-carboxylphenylazo) rhodanine (IRACP) were synthesized. Their absorbtion spectrum and fluorescence properties were studied.
So the synthesis of especial effective fluorescent reagents, establishing rapid, simple, high sensitive and selective methods for the determination of heavy metals and accomplishing the real-time, on-line, on-situ and automatic determination are of great value.
Hg(II) could react with the fluorescent reagent OVAQ (λex/em = 278/314 nm) to form a nonfluorescent complex in an ethanol-water medium of pH 6.00.
Separation and identification of aliphatic amine derivatives with a new fluorescent reagent using high-performance liquid chroma
Luminarin 9: A new fluorescent reagent for the precolumn derivatizatioin of main volatile and non-volatile N-nitrosamines in RP-
The fluorescent reagent 2-chloro-4-methoxy-6-(4-methoxy-1-naphthyl)-[1,3,5]triazine (CMMNT) has been synthesized and used for derivatization of mixtures of α-amino acids.
Excess reagent was extracted with n-hexane-ethyl acetate 9:1-10:1 (v/v); this enabled direct analysis using CE with no significant disturbance from the major fluorescent reagent degradation by-products.
Post-column derivatization withortho-phthalaldehyde made the assay more selective than when using pyrenemaleimide as thiol fluorogenic reagent.
An HPLC method for the determination of primary amino acids in tobacco, based on precolumn derivatization with the fluorogenic reagent naphthalene-2,3-dicarboxaldehyde, is described.
Asp and glu were derivatised using a fluorogenic reagent,o-phthaldialdehyde/2,3,4,6-tetra-O-acetyl-1-thio-β-D-glucopyranose (OPA/TATG).
The fluorogenic reagent 2-naphthyloxycarbonyl chloride (NOC-Cl) has been used for the automated precolumn derivatization of biogenic amines (BAs) at ambient followed by liquid-chromatographic separation of the derivatives formed.
Reduced plasma samples were derivatized using the thiol-specific fluorogenic reagent 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F) in borate borate buffer.