It indicates that mouse oviduct epithelial cell, modified M16 media and ES cell media with cardiomyocyte media can be more successfully applied in mouse somatic cell nuclear transfer and isolation of ES cell from reconstructed embryos.
Lymphocytes were collected from peripheral blood by lymphocyte isolation liquid (density 1.088), and transferred into enucleated oocytes by intracyto- plasmic injection. Two hours later, oocytes injected with donor cell nuclei were activated for 6 hours by treatment in modified M16 medium containing 10 mmol/L SrCl_2, and then cocultured with mouse oviduct epithelial cell in mM16 medium.
Results: Among the embryos co-cultured with oviductal epithelial cells and endometrium of endometriosis,25.9% (P>0.05) and 11.1% (P<0.05) developed to expanded blastocyst after 72 hrs to 96 hrs of culture in vitro,as compared with 25.7% and 26.2% respectively in the controls.
The different effects of cell monolayers on the maturation, fertilization and embryos development were researched according to the design when porcine ovarian cortex cells (pOCCs), porcine follicular granule cells (pFGCs), porcine oviductal epithelial cells (pOECs) and porcine cumulus cells (pCCs) were co-cultured individually with porcine oocytes, fertilized oocytes and embryos during IVM, IVF and IVC.
3. When MFFC, mouse oviductal epithelial cells (MOEC) and mouse granulosa cells (MGC) were transfected at passage 3, the transfection efficiency was 27.8%, 13.7% and 14.2%, respectively, under the described transfection conditions.
The results showed that embryos co-cultured with oviductal epithelial cell and granulosa cell achieved significantly higher blastocyst rate compared with the control group (P<0.05) and co-cultured with oviductal epithelial cell had more embryo cell number than those with granulosa cell.
PurposeThe present study was designed to pursue the kinetic characteristics of acrosome reaction(AR) of sperm in the fallopian tube in vivo, to evaluate the effect of human oviductal epithelial cell(LIOEC) on the AR capacity of sperm and the possible mechanisms through the application of coculture system with HOEC in vitro.
The results showed that the oviduct epithelial cells were the primary site of immunoreactive EGF,TGFα,HB-EGF,AR and EGFR,which were present to a lesser extent in the stromal cells,smooth muscle cell layers and serosal tissue.
The results showed that the oviduct epithelial cells were the primary site of immunoreactive LIF,LIFR and gp130,which were present to a lesser extent in the stromal cells,smooth muscle cell layers and serosal tissue.
2. To take KSOM and KSOMFBS as culture medium, the embryos separately co-culture with mouse oviduct epithelial cells (MOEC) or cumulus cells (CC). The changes of embryo numbers from 2-cell to 4-cell and blastocysts numbers were observed.
PGE2 production by oviductal epithelial cells is stimulated by PAF, and WEB-2086, a PAF-receptor blocker, specifically blocks the PAF-induced PGE2 production.
However, 5 h later a positive staining was observed in the oviduct ampulla and included the cumulus granulosa cells lying in the peripheral parts of postovulatory cumuli oophori, and the oviductal epithelial cells of this region.
Thus, the localization and the relative amount of SBG in the isthmus and ampulla of pig's oviduct are compatible with its possible function in sperm binding to oviductal epithelial cells.
Non-neuronal acetylcholine and choline acetyltransferase in oviductal epithelial cells of cyclic and pregnant pigs
In conclusion, we show expression of ChAT in oviductal epithelial cells at different stages of the oestrus cycle and pregnancy, indicating that these cells can synthesize ACh in a cycle-dependent manner.