A new method of preparation o-nitrobenzyl bromide using o-nitrotoluene as raw material, NaBrO3/NaHSO3 as bromination agent and AIBN as initiator was developed,under the optimum conditions n(o-nitrotoluene) ∶ n(sodium bromate) ∶ n(sodium bisulfite)=1 ∶ 4 ∶ 4,reaction terperature 70 ℃,reaction time 2 h,the yield is up to 73%.
The results show that in water under the conditions of n(hydrazine hydrate)∶n(o-nitrotoluene)=2∶1,the quantity of catalyst CuO/Al2O3 (n(CuO)∶n(Al2O3)=1∶2) 0.015g/mmol o-nitrotoluene, the reaction time for 50 min and the temperature of 80℃, the yield of o-toluidine is 98%.
The results showed that under the conditions of n(hydrazine hydrate)∶n(nitrotoluene)=2∶1,the quantity of catalyst FeO(OH) 0.015 g/mmol o-nitrotoluene and 50 min at 80℃ in water the yield of o-toluidine was 98%.
Under the optimal reaction conditions the reaction underwent for 3 h at 95 ℃ with A 1 as PTC in neutral reaction system, the mole ratio of potassium permanganate to O nitrotoluene was 2.5∶1, and the yield of ONBA was 95.0%.
The yield of 2 nitrobenzyl alcohol was 80% and the purity above 95% by using di(2 ethyl hexyl)peroxy dicarbonate catalyst under the optimum reaction condition,i. e. the molar ratio of 2 nitrotoluene to bromine was 2∶1,the bromination temperature was 64～66℃,the bromination time and the hydrolysis time were 2h and 16h respectively.
Indole was prepared by DMF di-n-propyl acetal and 2-nitrotoluene having reactive methyl, which involves using DMF di-n-propyl acetal instead of DMF dimethyl acetal to improve the reaction temperature, pyrrolidine as anti-polymerization agent, and reductive cyclization.
Limits of detection for 3-nitrophenol, nitrobenzene, 4 and 2-nitrotoluene were less than 60, 12, 60 and 280 ng L-1 respectively.
The Mn(III)/oxalate/O2 system was also able to transform other nitroaromatic compounds such as 2-nitrotoluene, 4-nitrotoluene, 2,4-dinitrotoluene, TNT - the latter to a lesser extent -, and their reduction derivatives.
The three strains are chemotactic toward 2,4,6-trinitrotoluene (TNT), 2,3-DNT, 2,4-DNT, 2,5-DNT, 2-nitrotoluene (NT), 4NT, and 4-methyl-5-nitrocatechol (4M5NC), but not toward 2,6-DNT.
The substrate specificities of the mutant enzymes were tested with the substrates indole, indoline, 2-nitrotoluene (2NT), naphthalene, biphenyl, and phenanthrene.
Active site residues controlling substrate specificity in 2-nitrotoluene dioxygenase from Acidovorax sp.