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免疫基因治疗
相关语句
  immune gene therapy
     Most of biotherapy methods are the study of tumor immune gene therapy on the principle of improving host immune response.
     目前的免疫基因治疗研究又多集中于细胞因子基因治疗。
短句来源
     immune gene therapy and receptor gene therapy.
     免疫基因治疗[6]; 受体基因治疗[7]等。
短句来源
     Conclusion:Cis-active element of α-fetoprotein gene can drive IFN-β gene specifically expressed in the human hepatoma cells,which present some valuable materials for the hepatoma-specific immune gene therapy.
     结论:AFP"持家基因"顺式调控序列在合成AFP的肝癌细胞中特异驱动IFN-β基因表达,该研究为肝癌特异性免疫基因治疗提供了资料。
短句来源
     New therapeutic approaches based on the advances in molecular biology and tumor immunology are expected to establish a more effective adjuvant therapy for gliomas. The active specific strategies of glioma immunotherapy including DC vaccines, heat shock proteins (HSPs) and molecular vaccines which are used to break the tolerance induced by glioma via cross immunologic reactions and the strategies of immune gene therapy involving proinflammatory factors such as B7, IFN-γ, GM-CSF have gained more attention and showed a promising prospect.
     随着分子生物学和肿瘤免疫学的发展,胶质瘤的主动特异性免疫治疗策略如树突状细胞(DC)疫苗、热休克蛋白(HSP)、通过交叉免疫反应打破肿瘤诱导的免疫耐受的分子疫苗以及B7、IFN-γ、GM-CSF等前炎性免疫基因治疗等已引起人们极大的注意并显示出令人鼓舞的应用前景。
短句来源
     The gene therapy for the breast cancer includes the gene flock,gene addition and immune gene therapy. It is confirmed that herceptin combined with chemotherapy can remarkbly improve the therapeutic effect of advanced metastatic breast cancer.
     乳腺癌的基因治疗主要包括癌基因封闭、基因添加、免疫基因治疗,已证实herceptin联合化疗能明显提高晚期复发转移乳腺癌的疗效。
短句来源
  immuno gene therapy
     Objective:Activation of murine spleen lympyocytes synergistically induced by B7 molecules and immunological factors was studied to explore immuno gene therapy of nonimmunogenic tumor.
     目的研究B7分子与免疫因子之间的协同作用,探索非免疫原性肿瘤的免疫基因治疗
短句来源
     Conclusions The expression of TGFβ 2 and TGFβ 1 was positively correlated with the tumor grade, so they could be a good target for immuno gene therapy of malignant gliomas.
     结论 TGFβ2 和TGFβ1表达水平与肿瘤恶性程度呈正相关 ,TGFβ2 和TGFβ1可作为恶性胶质瘤免疫基因治疗的候选基因。
短句来源
     Objective To explore immuno gene therapy of non immunogenic tumor.
     目的探索非免疫原性肿瘤的免疫基因治疗
短句来源
  “免疫基因治疗”译为未确定词的双语例句
     The experimental Study on SEA/B7.1 Immuogene Therapy to Murine Hepatoma
     小鼠肝癌SEA/B7.1免疫基因治疗的实验研究
短句来源
     Study on the Immunogenic Therapy for Osteosarcoma by Antisense TGF-β1 Gene
     应用反义TGF-β1基因进行骨肉瘤免疫基因治疗的研究
短句来源
     Studies of Immuno-gene Therapy of Cancer by MHC, B7 and IL-2 Gene Transfer and Preliminary Construction of Hepatocarcinoma Specific Gene Therapy System
     肿瘤的MHC、B7和IL-2及其联合的免疫基因治疗研究及肝癌特异性基因治疗系统的初步建立
短句来源
     Prevention of lymphoma with EL-4/GM-B7.1. C57BL/6 mice were inoculatedwith different times(one to three) tumor vaccines, and then the mice weresubsequently challenged with 5×104 wild-type EL-4 cells.
     (2)免疫基因治疗实验:用丝裂霉素 C 灭活的 EL-4、EL-4/pLXSN、EL-4/mGM-CSF、EL-4/mB7.1、EL-4/mGM-B7.1 细胞注射已用野生型 EL-4 细胞致瘤的 C57BL/6小鼠,观察淋巴瘤生长情况及小鼠存活时间。
短句来源
     Cancer Immuno-gene Therapy by Use of Adenovirus-mediated Glucose-regulated Protein 94 (GRP94) Expression
     复制缺陷型腺病毒介导糖调节蛋白GRP94表达应用于肿瘤免疫基因治疗
短句来源
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  immune gene therapy
In this review, we illustrate the recent developments in immune gene therapy.
      
Immune gene therapy of experimental mouse brain tumor with adenovirus-mediated gene transfer of murine interleukin-4
      
High transduction rates and the specific cytolysis of CD44v7/8-redirected CTLs are promising tools for an immune gene therapy approach for advanced cervical cancer.
      
Non-viral in vivo immune gene therapy of cancer: combined strategies for treatment of systemic disease
      
Progress reports on immune gene therapy for stage IV renal cell cancer using lethally irradiated granulocyte-macrophage colony-s
      
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Objective:To study the hepatoma cell-specific expression of human interferon gene mediated by retroviral vectors.Methods:Human interferon-β complementary DNA (IFN-β cDNA) was inserted into polylinker site of PMNSM retroviral vector to construct recombinant retroviral vector pMNSIFNB,in which the transcription of IFN-β gene was drived by SV40 early region promoter,and MNAIFNB,in which the transcription of IFN-β gene was drived by SV 40 early region promoter regulated by α-fetoprotein enhancer.The retroviral constructs...

Objective:To study the hepatoma cell-specific expression of human interferon gene mediated by retroviral vectors.Methods:Human interferon-β complementary DNA (IFN-β cDNA) was inserted into polylinker site of PMNSM retroviral vector to construct recombinant retroviral vector pMNSIFNB,in which the transcription of IFN-β gene was drived by SV40 early region promoter,and MNAIFNB,in which the transcription of IFN-β gene was drived by SV 40 early region promoter regulated by α-fetoprotein enhancer.The retroviral constructs were respectively introduced into PA317 amphotropic packaging cells by means of lipofect AMINETM mediated gene transfer procedure.The plasmids transfection efficiency was among(4 ̄25)×103 colonies/μg DNA/106 PA317 cells.The retrovirus infection efficiency was among(4.5 ̄500)×104 Coloney Forming Units(CFU)/ml.The recombinant retroviruses were used to infect human hepatoma cells,renal cell carcinoma cells and melanoma cell lines in the presence of 4 μg/ml polybrene.Results:Dot hybridization of total RNA from the neomycin resistant colonies and interferon expression assay indicated that human α-fetoprotein enhancer induced efficient and specific transcription and expression of IFN-β gene drived by the promoter of different origin in the human hepatoma cells in which α-fetoprotein was highly produced. Conclusion:Cis-active element of α-fetoprotein gene can drive IFN-β gene specifically expressed in the human hepatoma cells,which present some valuable materials for the hepatoma-specific immune gene therapy.

目的:研究逆转录病毒介导干扰素基因转移及在肝癌细胞中特异表达作用。方法:将人β干扰素(HuIFN-β)cDNA克隆到逆转录病毒载体PL位点,分别构建了转录受SV40启动子驱动的载体MNSIFNB和转录受人甲胎蛋白增强子(AFPe)调控的载体MNAIFNB。用脂质体转染法将载体分别转导PA317包装细胞,质粒转染率为每105PA317细胞(4~25)×103CFU/μg,病毒感染率(4.5~500)×104CFU/ml。重组病毒在4μg/mlpolybrene存在条件下感染人肝癌、肾癌及黑色素瘤细胞。结果:NeoR克隆RNA斑点杂交及IFN活性分析证明,人AFPe可促进异源启动子启动HuIFN-β基因在合成AFP的人肝癌细胞中高效特异转录和表达。结论:AFP"持家基因"顺式调控序列在合成AFP的肝癌细胞中特异驱动IFN-β基因表达,该研究为肝癌特异性免疫基因治疗提供了资料。

Inordertoinvestigatehepatoma-specificexpressionandspecificanti-hepatomaefectoftumornecrosisfactorgenes,weconstructedtheretroviralvectorsMNSTandMNATcontainingmurinetumornecrosisfactorgeneunderthetranscriptioncontrolofSV40earlyregionpromoterandalbuminenhancer/promoter,andtheretroviralvectorsLTSNandLTASNcontaininghumantumornecrosisfac-torgeneunderthetranscriptioncontrolofSV40earlyregionpromoterandhumanα-fetoproteinen-hancer/SV40earlyregionpromoter,respectively.TheconstructswereintroducedintoPA317celstopackageintorecombinantretroviruses,whichwereusedtoinfecttumorcellines.Bothofalbuminen-hancer/promoterandα-fetoprotinenhancerwereprovedtoinduceeficientandspecifictranscriptionandexpressionoftumornecrosisfactorgenesinhepatomacels.InsitugenetherapyusingretrovirusesMNATanditsproducingcelsPA317/MNATspecifcalyinducedregressionofexperimentalhepatoma.Itwasindicatedthatthetranscriptionalregulatorysequencesofthetumorcharacteristicproteins'“home-keeping”genesareofgreatsignificanceintumortissue-specificimmunegenetherapy....

Inordertoinvestigatehepatoma-specificexpressionandspecificanti-hepatomaefectoftumornecrosisfactorgenes,weconstructedtheretroviralvectorsMNSTandMNATcontainingmurinetumornecrosisfactorgeneunderthetranscriptioncontrolofSV40earlyregionpromoterandalbuminenhancer/promoter,andtheretroviralvectorsLTSNandLTASNcontaininghumantumornecrosisfac-torgeneunderthetranscriptioncontrolofSV40earlyregionpromoterandhumanα-fetoproteinen-hancer/SV40earlyregionpromoter,respectively.TheconstructswereintroducedintoPA317celstopackageintorecombinantretroviruses,whichwereusedtoinfecttumorcellines.Bothofalbuminen-hancer/promoterandα-fetoprotinenhancerwereprovedtoinduceeficientandspecifictranscriptionandexpressionoftumornecrosisfactorgenesinhepatomacels.InsitugenetherapyusingretrovirusesMNATanditsproducingcelsPA317/MNATspecifcalyinducedregressionofexperimentalhepatoma.Itwasindicatedthatthetranscriptionalregulatorysequencesofthetumorcharacteristicproteins'“home-keeping”genesareofgreatsignificanceintumortissue-specificimmunegenetherapy.

为探讨肿瘤坏死因子基因对肝癌的治疗作用,分别构建SV40早期启动子和白蛋白增强子/启动子调控小鼠肿瘤坏死因子基因的逆转录病毒载体MNST和MNAT,以及SV40早期启动子和人甲胎蛋白增强子/SV40启动子调控的逆转录病毒载体LTSN和LTASN。构建物导入PA317细胞中包装成重组病毒,感染肿瘤细胞,证明白蛋白增强子/启动子和甲胎蛋白增强子均能使肿瘤坏死因子基因在肝癌细胞中高效特异表达。MNAT病毒和PA317/MNAT产病毒细胞invivo法基因治疗有特异抗肝癌效果。提示组织特征蛋白“持家基因”转录调控序列在肿瘤组织特异性免疫基因治疗研究中有重要意义。

Objective To investigate the differences of tumorigenicity and metastasis of murine tumors with different immunogenicity by B7.1gene transfer.Method Using retroviral-mediated gene ...

Objective To investigate the differences of tumorigenicity and metastasis of murine tumors with different immunogenicity by B7.1gene transfer.Method Using retroviral-mediated gene transfer,we transduced B7.1cDNA into a panel of murine tumor lines with different immunogenicity to study the ef fect of B7.1 costimulation on antitumor immunity.Results After transduced with B7.1 cDNA.the immunogenic T lymphoma EL4 regressed completely,and tumorgenicity of three nonimmunogenic tu mors melanoma B16,mastocytoma P815 and mammary adenocarcinoma MA891 were significantly re duced in syngeneic mice.The experimental metastasis of B16 and spontaneous pulmonary metastasis of MA891 were profoundly suppressed.Moreover,immunization with B7.1 cDNA transduced B16 in duced systemic immunity against subsequently inoculated parental B16 tumor,while this immunization method did not provide protective immunity against established parental B16 tumor.Conclusion Our results show that varied extent of antitumor immunity can be induced to abrogate or decrease tumori genecity and metastasis through B7.1 gene transduction,depending on the immunogenic potential of tu mors.

为B71分子基因在肿瘤免疫基因治疗中的应用提供实验依据。方法通过逆转录病毒介导的基因转移技术,将B71cDNA导入具有不同免疫原性的小鼠肿瘤细胞获得表达,并观察转基因细胞在纯系小鼠体内生长和转移的改变。结果由于B7-1基因的转导表达,使具有免疫原性的EL4T淋巴瘤细胞致瘤性丧失;使非免疫原性的B16黑色素瘤、P815肥大细胞瘤和MA891乳腺癌细胞的致瘤性减弱;使B16细胞的实验性转移和MA891细胞的自发肺转移能力降低。通过转导B7-1基因的B16细胞的免疫接种,可使再次接种的亲本B16细胞的致瘤性减弱:但对已生长的亲本B16细胞影响不明显。结论B71基因导入不同的肿瘤细胞后,可引起机体产生不同程度抗肿瘤免疫反应,使肿瘤细胞在体内的生长和转移能力丧失或减弱,这种反应的强弱与肿瘤细胞本身的特性有关。

 
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