助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   过氧化物酶基因 在 水产和渔业 分类中 的翻译结果: 查询用时:0.059秒
图标索引 在分类学科中查询
所有学科
水产和渔业
生物学
植物保护
农作物
园艺
农业基础科学
外科学
妇产科学
中药学
更多类别查询

图标索引 历史查询
 

过氧化物酶基因
相关语句
  peroxidase genes
    Forty eight clones were sequenced,including 16 new genes,10 glutathione-s-transferase genes,5 glutathione peroxidase genes,2 catalase genes,3 hydroxysteroid dehydrogenase genes,4 tyrosine kinase genes,1 thyroid peroxidase-like protein gene,3 genes for cellulose,1 cAMP responsive element binding protein gene and 3 shell biomineralization related genes.
    选取含插入片段大小不同的48个克隆测序,其中有16个新基因,10个谷胱甘肽转移酶的基因,5个谷胱甘肽过氧化物酶基因,2个过氧化氢酶基因,3个羟基类固醇脱氢酶基因,4个酪氨酸激酶基因,1个类甲状腺过氧化物酶蛋白基因,3个纤维素酶基因,1个cAMP效应元件结合蛋白基因,3个贝壳生物矿化相关蛋白的基因。
短句来源
查询“过氧化物酶基因”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  peroxidase genes
Expressed sequence tags (EST) analysis of all putative peroxidase genes of A.
      
Intraspecific polymorphism in peroxidase genes located on Arabidopsis thaliana chromosome 5
      
Nucleotide sequences of five peroxidase genes AtPrx52-AtPrx56 located on the upper arm of chromosome 5 were comparatively analyzed for six Arabidopsis thaliana ecotypes and lines (Columbia, Dijon-M, Blanes-M, Enkheim-M, Ler, and K-156).
      
Polymorphism of the Catalase and Glutathione Peroxidase Genes and Macrovascular Complications in Non-Insulin-Dependent Diabetic
      
Results suggest that these species may be closely related in phylogeny, and/or geographic isolation changes the peroxidase genes through mutation and selection.
      
更多          


Screening of a cDNA library from Porphyra yezoensis with probe based upon cytosolic ascorbate peroxidase (APX,EC 1.11.1.11) genes identified a full-length clone apparently encoding a novel type of ascorbate peroxidase.The clone contained an open reading frame for a protein of 242 amino acids,which exhibited sequence similarity to known cytosolic APX from higher plants and contained two motifs conserved in peroxidases.The deduced amino acid sequence of P.yezoensis APX showed a hybrid-type structure,which was...

Screening of a cDNA library from Porphyra yezoensis with probe based upon cytosolic ascorbate peroxidase (APX,EC 1.11.1.11) genes identified a full-length clone apparently encoding a novel type of ascorbate peroxidase.The clone contained an open reading frame for a protein of 242 amino acids,which exhibited sequence similarity to known cytosolic APX from higher plants and contained two motifs conserved in peroxidases.The deduced amino acid sequence of P.yezoensis APX showed a hybrid-type structure,which was only found previously in an APX-B from a unicellular red alga Galdieria partite,similar to both chloroplastic and cytosolic isoforms of higher plants.It was possible that the hybrid-type structure was an important characteristic of red algal APX.APX cDNA from P.yezoensis showed a high (G+C) content (88.4% in the third-base position and 63.7% total) in the coding region.The nucleotide sequence data reported in this paper will appear in the GenBank database under accession number AY282755.

利用细胞质抗坏血酸过氧化物酶(APX)基因部分片段为探针,从条斑紫菜(Porhyrayezoensis)叶状体的cDNA文库中筛选出1种新型的编码APX的全长cDNA克隆。该克隆开放阅读框编码242个氨基酸,与高等植物已知的胞质APX序列相似,同时具有两个特征性的保守区域。推导的氨基酸序列具有与在一种单细胞红藻(Galdieriapartite)中发现的APX B相似的杂交类型结构,这种结构可能是红藻或者非绿色光合生物的APX的重要特征。该cDNA克隆编码区G+C含量达到63 7%,其中密码子的第3位G+C含量高达88 4%。本实验得到的抗坏血酸过氧化物酶基因的cDNA序列已登录到GenBank数据库中,登录序号为:AY282755。

A study was conducted to clone the differentially expressed genes in the mantle of abalone Haliotis discus hannai during vitamin E deficiency.Juvenile abalone [initial weight(0.71±0.00)g;initial shell length(15.49±0.04)mm] were fed 240 d with semi-purified diets containing 2 levels of vitamin E:3.5 mg/kg and 52.8 mg/kg.The cDNA subtractive library of the mantle of abalone vitamin E deficiency-treated was constructed using the method of suppression subtractive hybridization(SSH).The results showed that the constructed...

A study was conducted to clone the differentially expressed genes in the mantle of abalone Haliotis discus hannai during vitamin E deficiency.Juvenile abalone [initial weight(0.71±0.00)g;initial shell length(15.49±0.04)mm] were fed 240 d with semi-purified diets containing 2 levels of vitamin E:3.5 mg/kg and 52.8 mg/kg.The cDNA subtractive library of the mantle of abalone vitamin E deficiency-treated was constructed using the method of suppression subtractive hybridization(SSH).The results showed that the constructed cDNA subtractive library was a highly efficient one:the cDNAs were significantly enriched for differentially expressed sequences.One hundred positive clones were randomly picked and identified by PCR method;95% clones contained 100-900 bp inserts,which might be the cDNA fragments of differentially expressed genes in vitamin E deficiency group.Forty eight clones were sequenced,including 16 new genes,10 glutathione-s-transferase genes,5 glutathione peroxidase genes,2 catalase genes,3 hydroxysteroid dehydrogenase genes,4 tyrosine kinase genes,1 thyroid peroxidase-like protein gene,3 genes for cellulose,1 cAMP responsive element binding protein gene and 3 shell biomineralization related genes.In conclusion,the method to construct cDNA subtractive library could well reflect the gene information of abalone affected by vitamin E.Results of the present study provide the basal data to research of relationship between nutrients and gene expression of abalone.

为了克隆维生素E缺乏时皱纹盘鲍(Haliotis discus hannai)外套膜差异性表达的基因,本研究配制了维生素E缺乏水平(3.5 mg/kg)和正常添加水平(52.8 mg/kg)的人工饲料,喂养皱纹盘鲍幼鲍,初始体质量为(0.71±0.00)g,初始壳长为(15.49±0.04)mm,240 d后,采用抑制消减杂交法(Suppression Subtractive Hybridization,SSH)构建了维生素E缺乏组与正常组外套膜组织差异表达的cDNA消减文库。经检验,差异表达的基因均被富集了25-28倍,证明构建的cDNA消减文库具有很强的消减效率。从文库中随机挑取100个白色克隆摇菌液进行PCR鉴定,95%的克隆中均有100-900 bp的插入片段,这些片段可能是维生素E缺乏差异表达基因的cDNA片段。选取含插入片段大小不同的48个克隆测序,其中有16个新基因,10个谷胱甘肽转移酶的基因,5个谷胱甘肽过氧化物酶基因,2个过氧化氢酶基因,3个羟基类固醇脱氢酶基因,4个酪氨酸激酶基因,1个类甲状腺过氧化物酶蛋白基因,3个纤维素酶基因,1个cAMP效应元件结合蛋白基因,3个贝壳生物矿...

为了克隆维生素E缺乏时皱纹盘鲍(Haliotis discus hannai)外套膜差异性表达的基因,本研究配制了维生素E缺乏水平(3.5 mg/kg)和正常添加水平(52.8 mg/kg)的人工饲料,喂养皱纹盘鲍幼鲍,初始体质量为(0.71±0.00)g,初始壳长为(15.49±0.04)mm,240 d后,采用抑制消减杂交法(Suppression Subtractive Hybridization,SSH)构建了维生素E缺乏组与正常组外套膜组织差异表达的cDNA消减文库。经检验,差异表达的基因均被富集了25-28倍,证明构建的cDNA消减文库具有很强的消减效率。从文库中随机挑取100个白色克隆摇菌液进行PCR鉴定,95%的克隆中均有100-900 bp的插入片段,这些片段可能是维生素E缺乏差异表达基因的cDNA片段。选取含插入片段大小不同的48个克隆测序,其中有16个新基因,10个谷胱甘肽转移酶的基因,5个谷胱甘肽过氧化物酶基因,2个过氧化氢酶基因,3个羟基类固醇脱氢酶基因,4个酪氨酸激酶基因,1个类甲状腺过氧化物酶蛋白基因,3个纤维素酶基因,1个cAMP效应元件结合蛋白基因,3个贝壳生物矿化相关蛋白的基因。总的来说,利用抑制消减杂交的方法构建差异表达cDNA文库,可以比较好地反映维生素E对皱纹盘鲍影响的基因信息,为研究其他营养素对皱纹盘鲍基因表达的影响提供了基础数据。

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关过氧化物酶基因的内容
在知识搜索中查有关过氧化物酶基因的内容
在数字搜索中查有关过氧化物酶基因的内容
在概念知识元中查有关过氧化物酶基因的内容
在学术趋势中查有关过氧化物酶基因的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社