Using either primer pair 5--ACCATCGTCAACCACTACATCG-3' and 5'-GCTGCCAGAAACCCACGTCAT-3' or 5'-ACCATCGTCAACCACTACATCG-3' and 5'- GAGGTCGTCCGTCCACTCCTG-3' could amplify specific segment of bar gene cloned in the transgenic plants.
METHODS: Plant expression plasmid pROSB carrying the gene encoding chimera SBR-CT~(ΔA1) and bar gene was transferred into Agrobacterium tumefaciens LBA4404 with the Freeze-thaw method,then recombinant Agrobacterium tumefaciens AT-RSB(pROSB、LAB4404) was transformed into tomato cotyledons by leaf discs method,and transgenic seedlings were identified by PCR and RT-PCR.
CONCLUSIONS: Transgenic tomato seedlings carrying the gene encoding chimera SBR-CT~(ΔA1) and bar gene were obtained,which may provide useful experimental foundation for further study on edible vaccine against caries.
The complete sequence of the gene bar was amplified by PCR from the plasmid pCAMBIA1300-bar and cloned into the recombinant expression recombinant pET28a(+) at the BamHⅠ and HindⅢ.
A new rice variety "E32HR" which was resistant to herbicide "Basta" was bred by transferring gene bar from the donor, a genetically modified organism "HR" obtained by biolistic method, into the breeding line "E32", a restorer of the super hybrid rice "65396" (Peiai 64S/E32) through successive backcrossing for four times.
Transgenic bar-gene wheat 87158(BG 87) and 98005(BG 98) are planted at pot as experiment material as well their parent wheat Yangmai 87158(YM 87) and Annong 98005(AN 98) as control. Effects of bar-gene on rhizosphere microbes of wheat were studied through analyzing the change of microbial flora in reizosphere soil at different wheat growth stages.
From elongation stage to late growth time,there is a significant difference on quantity of bacteria and fungi in rhizosphere soil between bar-gene wheat and control while there is no difference on quantity of actinomycete.
For this purpose, we have constructed a recombinant Ti plasmid, in which the maize Ds sequence and a bar gene were ligated into the T DNA of pCAMBIA1300 to form a Ti plasmid pDsBar1300 (Fig.1).
binary vectorpDLBRBbarm which carried two independent T-DNAs, one containing a selectable marker neomycinphosphotransferase (nptⅡ) gene and the other a bar gene, was constructed.
By the transformation ~~ith an expression plasmid that h. 3s a MAR-transgene-MAR structure by inserting two MARs at both sides of a bar gene, we tested the influence of MAR sequence mediattion on transgene expression in tr~tnsgenic rice.
The regenerated plantlets harboring a HVA1 gene and a bar gene were used as experiment materials, this studies are carred out from two aspects,one includes herbicide resistance anlysis .
Three systems (gradation, delayed, and regeneration) for in vitro selection of transgenic wheat tissue using the bar gene, providing resistance to the herbicide phosphinothricin (PPT), were compared.
violaceus plants that had been previously transformed by a vector containing the maize transposon system Spm/dSPm with bar gene located within the nonautonomous transposon.
Northern blotting hybridizations showed that the bar transcript was most abundant in cells grown in 2 mol/L NaCl, and less abundant in 0.5 mol/L NaCl, indicating that expression of the bar gene was induced at high salinity.
We utilized gene transfer technology for genetic perennial ryegrass improvement, efficient regeneration, and Agrobacterium-mediated transformation of phosphinothricin acetyltransferase gene (bar).
The unselectable gene (uidA) and the selectable gene (bar), on two separate plasmids, were transferred to maize (Hi-II derivative) by particle bombardment of embryogenic calli or suspension cells.
A 640-bp DNA fragment upstream to the start codon was employed to drive the expression of the reporter protein sGFP or a dominant selectable marker, the gene bar (resistance to ammonium glufosinate).
Hartog) were electroporated in the presence of plasmid pEmuGN and/or pEmuPAT, which contained the reporter gene gus and selectable marker gene bar, respectively.
Hypocotyl explants from young seedlings cocultivated with agrobacteria carrying a bar gene were selected on shoot-inducing media containing different concentrations of phosphinothricin (PPT) which is an active component of bialaphos.
The resulting plasmid, pNIP-GUS, was transformed into Norway spruce (Picea abies L.) embryogenic cultures by co-bombarding with a plasmid containing a bar gene construct as a selectable marker.
Three AC Karma plants (433, 436, and 437) carrying plasmid pRC62 containing a gus:npt fusion gene, and one Hy417 plant (438) carrying plasmid pBARGUS containing a bar gene and a gusA gene were recovered and characterized.
A resulting construct with a Bar gene cassette for bialaphos selection in plant (rpE-VP2) was introduced into Agrobacterium tumefaciensby electroporation.
The Agrobacterium strain used was LBA4404 carrying a `Super-binary' vector with a bar gene as a selectable marker for herbicide resistance in the plant cells.
Rice is one of the important crops in the world. Research on rice is emphasized in many coutries. Biotechnology opens a new way for crop improvement. The protoplasts isolated from rice "02428" were electroporated with plasmid pFEWZ16 containing CaMV 35S promoter/bar chimaeric gene and Act1 promoter/B. t chimaeric gene. PPT' calli were formed from protoplasts electroporated with plasmid pFWZ16. The calli could grew normally on MS4P8 medium, and differentiated on N6 medium. Some plants regenerated from the PP...
Herbicide resistant transgenic upland rice plants were recovered using biolistic method. Upland rice suspension cells and immature embryos were transformed by particle bombardment with a plasmid containing herbicide resistant bar gene. One week after bombardment, the bombarded cells were transferred to N6 medium with phosphinothricin (PPT) for selection. Fertile transgenic plants were regenerated from PPT resistant calli. Integration of bar gene was confirmed by Southern blot hybridization. Resistance of ...
Plasmid pCB1, containing bar gene under the control of CaMV 35s promoter was introduced to rice immature embryo using biolistic method. Three basta resistant transgenic rice plants were regenerated after selection. The integration of the bar gene in the genome of the transgenic rice plants was shown by PCR and Southern analysis. RNA dot analysis demonstrated the mRNA expression of the bar gene at transcriptional level in transgenic plants. Among 321 T 1 progenies of transgenic plant JY119 2, 274 plants s...
bar基因
bar gene
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