助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   bar基因 的翻译结果: 查询用时:0.047秒
图标索引 在分类学科中查询
所有学科
农作物
生物学
园艺
农业基础科学
基础医学
更多类别查询

图标索引 历史查询
 

bar基因     
相关语句
  bar gene
     Using either primer pair 5--ACCATCGTCAACCACTACATCG-3' and 5'-GCTGCCAGAAACCCACGTCAT-3' or 5'-ACCATCGTCAACCACTACATCG-3' and 5'- GAGGTCGTCCGTCCACTCCTG-3' could amplify specific segment of bar gene cloned in the transgenic plants.
     用引物对5′-ACCATCGTCAACCACTACATCG-3′和5′-GAGGTCGTCCGTCCACTCCTG-3′或5′-ACCATCGTCAACCACTACATCG-3′和5′-GCTGCCAGAAACCCACGTCAT-3′分别可以扩增出转基因水稻99-1的bar基因特异片段420 hp和 86 hp。
短句来源
     METHODS: Plant expression plasmid pROSB carrying the gene encoding chimera SBR-CT~(ΔA1) and bar gene was transferred into Agrobacterium tumefaciens LBA4404 with the Freeze-thaw method,then recombinant Agrobacterium tumefaciens AT-RSB(pROSB、LAB4404) was transformed into tomato cotyledons by leaf discs method,and transgenic seedlings were identified by PCR and RT-PCR.
     方法:将含有携带编码嵌合体SBR-CTΔA1基因和bar基因片段的重组质粒pROSB经冻融法转化入农杆菌LBA4404,用叶盘转化法将重组农杆菌AT-RSB转化番茄子叶,得到转基因番茄株,用PCR及RT-PCR鉴定转化株。
短句来源
     AIM:To transform the gene encoding chimera SBR-CT~(ΔA1) and bar gene into tomato,and to obtaine the transgenic tomato.
     目的:将携带有编码嵌合体SBR-CTΔA1基因和bar基因的片段转入番茄,获得转基因番茄植株。
短句来源
     The Effects of the Insert Locus of Bar Gene on the Transgenic Wheat and Primary Research on Transgened Bar the AnNong 98005
     Bar基因的插入位点对其转基因小麦的影响及转育Bar基因安农98005的研究
短句来源
     CONCLUSIONS: Transgenic tomato seedlings carrying the gene encoding chimera SBR-CT~(ΔA1) and bar gene were obtained,which may provide useful experimental foundation for further study on edible vaccine against caries.
     结论:获得含嵌合体SBR-CTΔA1和bar基因的转基因番茄株,为可食用防龋疫苗的研究提供实验基础。
短句来源
更多       
  gene bar
     The complete sequence of the gene bar was amplified by PCR from the plasmid pCAMBIA1300-bar and cloned into the recombinant expression recombinant pET28a(+) at the BamHⅠ and HindⅢ.
     用BamH和Hind酶切,将bar基因与原核表达载体pET28a(+)连接,构建成重组质粒pET28bar。
短句来源
     Heredity of Abnormal Segregation Foreign Gene bar in Herbicide Resistant Rice 9311HR
     外源基因异常分离的抗除草剂水稻9311HR bar基因的遗传传递
短句来源
     cryIA(b)gene inherited and expressed together with the marker gene bar.
     在杂交后代中抗除草剂bar基因和目的基因cryIA(b)紧密连锁与协同表达 ;
短句来源
     PCR and PCR_Southern blot analysis suggested that the foreign gene bar had been transferred into wheat plants.
     对抗性植株进行PCR和PCR_Southern杂交检测 ,初步确定bar基因已导入小麦基因组。
短句来源
     A new rice variety "E32HR" which was resistant to herbicide "Basta" was bred by transferring gene bar from the donor, a genetically modified organism "HR" obtained by biolistic method, into the breeding line "E32", a restorer of the super hybrid rice "65396" (Peiai 64S/E32) through successive backcrossing for four times.
     以基因枪法转化获得的转bar基因水稻"HR"为供体亲本,两系法亚种间杂交稻优势组合"65396"的恢复系"E32"为受体亲本,经过4次回交转育成对除草剂Basta具有稳定抗性的水稻新品系"E32HR"。
短句来源
更多       
  bar-gene
     Transgenic bar-gene wheat 87158(BG 87) and 98005(BG 98) are planted at pot as experiment material as well their parent wheat Yangmai 87158(YM 87) and Annong 98005(AN 98) as control. Effects of bar-gene on rhizosphere microbes of wheat were studied through analyzing the change of microbial flora in reizosphere soil at different wheat growth stages.
     在盆栽实验条件下,以转bar基因小麦87158(BG87)、98005(BG98)和对照小麦扬麦87158(YM87)、安农98005(AN98)为材料,对小麦各生育期的根际微生物进行分析,研究bar基因对小麦根际微生物的影响。
短句来源
     Effects of bar-gene on microbial flora in wheat rhizosphere soil
     bar基因对盆栽小麦根际微生物的影响
短句来源
     From elongation stage to late growth time,there is a significant difference on quantity of bacteria and fungi in rhizosphere soil between bar-gene wheat and control while there is no difference on quantity of actinomycete.
     转bar基因小麦和对照小麦根际细菌和真菌的数量自孕穗期开始差异极显著,而放线菌的数量差异不明显。
短句来源
     bar-gene has no effects on microbes′ species in rhizosphere soil.
     转bar基因小麦根际细菌和真菌的主要类群与对照小麦基本相同。
短句来源
  a bar gene
     Regeneration of Transgenic Indica Rice Plants with a Cecropin B Gene and a bar Gene
     转抗菌肽B基因和bar基因籼稻植株的再生
短句来源
     For this purpose, we have constructed a recombinant Ti plasmid, in which the maize Ds sequence and a bar gene were ligated into the T DNA of pCAMBIA1300 to form a Ti plasmid pDsBar1300 (Fig.1).
     为此目的 ,将玉米的Ds因子及bar基因连接至载体pCAMBIA130 0的T DNA区域中 ,构建成重组Ti质粒pDsBar130 0。
短句来源
     binary vectorpDLBRBbarm which carried two independent T-DNAs, one containing a selectable marker neomycinphosphotransferase (nptⅡ) gene and the other a bar gene, was constructed.
     载体中,选择标记nptⅡ基因和另一代表外源基因的bar基因分别位于2个独立的T-DNA。
短句来源
     By the transformation ~~ith an expression plasmid that h. 3s a MAR-transgene-MAR structure by inserting two MARs at both sides of a bar gene, we tested the influence of MAR sequence mediattion on transgene expression in tr~tnsgenic rice.
     将MAR序列插入到bar基因两侧,构建具有“MAR-transgene-MAR”结构的表达质粒来转化受体材料,考察了MAR序列介导对转基因水稻中转基因表达的影响。
短句来源
     The regenerated plantlets harboring a HVA1 gene and a bar gene were used as experiment materials, this studies are carred out from two aspects,one includes herbicide resistance anlysis .
     本实验以基因枪介导获得的水稻再生植株(HVA1为目的基因,Bar基因为筛选标记)为材料,从两个方面进行了研究。
短句来源

 

查询“bar基因”译词为其他词的双语例句

     

    查询“bar基因”译词为用户自定义的双语例句

        我想查看译文中含有:的双语例句
    例句
    为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
      bar gene
    Three systems (gradation, delayed, and regeneration) for in vitro selection of transgenic wheat tissue using the bar gene, providing resistance to the herbicide phosphinothricin (PPT), were compared.
          
    violaceus plants that had been previously transformed by a vector containing the maize transposon system Spm/dSPm with bar gene located within the nonautonomous transposon.
          
    Stable expression of the promoterless bar gene in transformed rapeseed plants
          
    Expression of a synthetic cholera toxin B subunit in tobacco using ubiquitin promoter and bar gene as a selectable marker
          
    Northern blotting hybridizations showed that the bar transcript was most abundant in cells grown in 2 mol/L NaCl, and less abundant in 0.5 mol/L NaCl, indicating that expression of the bar gene was induced at high salinity.
          
    更多          
      gene bar
    We utilized gene transfer technology for genetic perennial ryegrass improvement, efficient regeneration, and Agrobacterium-mediated transformation of phosphinothricin acetyltransferase gene (bar).
          
    The unselectable gene (uidA) and the selectable gene (bar), on two separate plasmids, were transferred to maize (Hi-II derivative) by particle bombardment of embryogenic calli or suspension cells.
          
    The system was successfully applied to integrating phosphinothricin resistance gene bar and enhanced green fluorescence protein gene egfp into B.
          
    A 640-bp DNA fragment upstream to the start codon was employed to drive the expression of the reporter protein sGFP or a dominant selectable marker, the gene bar (resistance to ammonium glufosinate).
          
    Hartog) were electroporated in the presence of plasmid pEmuGN and/or pEmuPAT, which contained the reporter gene gus and selectable marker gene bar, respectively.
          
    更多          
      bar-gene
    As a control for this complementation experiment, Tpac1-7.2 was also transformed with an empty bar-gene vector, pBARGEM7.1.
          
      a bar gene
    Hypocotyl explants from young seedlings cocultivated with agrobacteria carrying a bar gene were selected on shoot-inducing media containing different concentrations of phosphinothricin (PPT) which is an active component of bialaphos.
          
    The resulting plasmid, pNIP-GUS, was transformed into Norway spruce (Picea abies L.) embryogenic cultures by co-bombarding with a plasmid containing a bar gene construct as a selectable marker.
          
    Three AC Karma plants (433, 436, and 437) carrying plasmid pRC62 containing a gus:npt fusion gene, and one Hy417 plant (438) carrying plasmid pBARGUS containing a bar gene and a gusA gene were recovered and characterized.
          
    A resulting construct with a Bar gene cassette for bialaphos selection in plant (rpE-VP2) was introduced into Agrobacterium tumefaciensby electroporation.
          
    The Agrobacterium strain used was LBA4404 carrying a `Super-binary' vector with a bar gene as a selectable marker for herbicide resistance in the plant cells.
          
    更多          


    Rice is one of the important crops in the world. Research on rice is emphasized in many coutries. Biotechnology opens a new way for crop improvement. The protoplasts isolated from rice "02428" were electroporated with plasmid pFEWZ16 containing CaMV 35S promoter/bar chimaeric gene and Act1 promoter/B. t chimaeric gene. PPT' calli were formed from protoplasts electroporated with plasmid pFWZ16. The calli could grew normally on MS4P8 medium, and differentiated on N6 medium. Some plants regenerated from the PPT...

    Rice is one of the important crops in the world. Research on rice is emphasized in many coutries. Biotechnology opens a new way for crop improvement. The protoplasts isolated from rice "02428" were electroporated with plasmid pFEWZ16 containing CaMV 35S promoter/bar chimaeric gene and Act1 promoter/B. t chimaeric gene. PPT' calli were formed from protoplasts electroporated with plasmid pFWZ16. The calli could grew normally on MS4P8 medium, and differentiated on N6 medium. Some plants regenerated from the PPT callus showed the resistant to 125 mg-L-1 PPT. They grew healthily after PPT treatment on their leaves, whereas all the plants from "02428"protoplasts electroporated without plasmid pFWZ16 and without PPT selection died. Data from PCR analysis and PCR -Southern assays showed two kinds of PPT resistant transgenic rice plants were obtained: (1)transgenic rice with bar gene but without B. t gene.(2)trans-genie rice with bar and B. t genes. The advantages of the wide compatibility cultivar "02428" used in this study and the potential application of transgenic plants to rice improvement were discussed.

    利用水稻“02428”原生质体进行遗传转化,转化原生质体的电击条件为:T_B=0.2s,CY=20,A=10kV,D=1mm,N_p=2~5~2~8。转化的质粒pFWZ16DNA含有CaMV35S-bar基因和Actl—B.t基因,电击后的原生质体培养过程中利用PPT(2~8mg·L~(-1))进行选择。选择6~10周后,在处理的原生质体中,再生出少量生长正常的愈伤组织,而阴性对照中再生的愈伤组织生长全部被抑制以至死亡。将经PPT选择生长正常的愈伤组织的一部分转到MS4P8培养基上,愈伤组织生长正常,而阴性对照全部变褐死亡;将经PPT选择生长正常的另一部分愈伤转到N6培养基上,1个月后再生出绿色植株。转到大田的绿色再生植株生长正常,经转化再生的植株涂125mg·L~(-1)的PPT后,植株7天后生长正常,而对照则失绿变黄,皱缩死亡。用PCR法、PCR—Southern法检测抗PPT的植株和愈伤是否含有Bar基因、B.t基因,实验结果说明本实验获得了两类抗除草剂草丁膦的水稻转基因植株。第一类含bar基因,第二类既含有bar基因也含有B.t基因。关于第二类转基因水稻...

    利用水稻“02428”原生质体进行遗传转化,转化原生质体的电击条件为:T_B=0.2s,CY=20,A=10kV,D=1mm,N_p=2~5~2~8。转化的质粒pFWZ16DNA含有CaMV35S-bar基因和Actl—B.t基因,电击后的原生质体培养过程中利用PPT(2~8mg·L~(-1))进行选择。选择6~10周后,在处理的原生质体中,再生出少量生长正常的愈伤组织,而阴性对照中再生的愈伤组织生长全部被抑制以至死亡。将经PPT选择生长正常的愈伤组织的一部分转到MS4P8培养基上,愈伤组织生长正常,而阴性对照全部变褐死亡;将经PPT选择生长正常的另一部分愈伤转到N6培养基上,1个月后再生出绿色植株。转到大田的绿色再生植株生长正常,经转化再生的植株涂125mg·L~(-1)的PPT后,植株7天后生长正常,而对照则失绿变黄,皱缩死亡。用PCR法、PCR—Southern法检测抗PPT的植株和愈伤是否含有Bar基因、B.t基因,实验结果说明本实验获得了两类抗除草剂草丁膦的水稻转基因植株。第一类含bar基因,第二类既含有bar基因也含有B.t基因。关于第二类转基因水稻的抗虫性有待测定。本工作利用生产上有重要应用价值的水稻广亲和品种“02428”,得到转基因植株,属首次报道。

    Herbicide resistant transgenic upland rice plants were recovered using biolistic method. Upland rice suspension cells and immature embryos were transformed by particle bombardment with a plasmid containing herbicide resistant bar gene. One week after bombardment, the bombarded cells were transferred to N6 medium with phosphinothricin (PPT) for selection. Fertile transgenic plants were regenerated from PPT resistant calli. Integration of bar gene was confirmed by Southern blot hybridization. Resistance of R0...

    Herbicide resistant transgenic upland rice plants were recovered using biolistic method. Upland rice suspension cells and immature embryos were transformed by particle bombardment with a plasmid containing herbicide resistant bar gene. One week after bombardment, the bombarded cells were transferred to N6 medium with phosphinothricin (PPT) for selection. Fertile transgenic plants were regenerated from PPT resistant calli. Integration of bar gene was confirmed by Southern blot hybridization. Resistance of R0 transgenic plants to herbicide PPT was shown by Basta (commercial form of PPT) applications in the greenhouse. Inheritance of bar gene was demonstrated by PCR analysis of R1 transgenic plants.

    以旱稻丹粳旱5-55、6-24、6-37(OryzasativaL.varjaponica)的悬浮细胞及未成熟胚为受体材料,采用基因枪法将含BAR基因的pDM302质粒DNA导入旱稻细胞中,经PPT筛选获得抗性愈伤组织,筛选出的愈伤组织在分化培养基上再生出完整的旱稻转基因植株。Southern分子杂交分析表明,外源BAR基因已整合到水稻基因组内,抗除草剂试验结果表明,转化植株对0.01%Basta(有效成分为PPT)有一定程度的抗性,说明外源BAR基因已在旱稻细胞内正确表达。90%的转基因植株可育并已结实,部分R1代植株PCR扩增结果证明外源BAR基因已遗传给后代

    Plasmid pCB1, containing bar gene under the control of CaMV 35s promoter was introduced to rice immature embryo using biolistic method. Three basta resistant transgenic rice plants were regenerated after selection. The integration of the bar gene in the genome of the transgenic rice plants was shown by PCR and Southern analysis. RNA dot analysis demonstrated the mRNA expression of the bar gene at transcriptional level in transgenic plants. Among 321 T 1 progenies of transgenic plant JY119 2, 274 plants showed...

    Plasmid pCB1, containing bar gene under the control of CaMV 35s promoter was introduced to rice immature embryo using biolistic method. Three basta resistant transgenic rice plants were regenerated after selection. The integration of the bar gene in the genome of the transgenic rice plants was shown by PCR and Southern analysis. RNA dot analysis demonstrated the mRNA expression of the bar gene at transcriptional level in transgenic plants. Among 321 T 1 progenies of transgenic plant JY119 2, 274 plants showed a high resistance to herbicide Basta, while the other 47 plants were sensitive. According to the Southern analysis of 8 randomly selected plants from those 274 Basta resistant plants, the bar gene has been stably inherited in T 1 progenies.

    利用基因枪转化系统,将含有由CaMV35S启动子启动的bar基因的转化质粒pCB1导入水稻幼胚,经筛选、再生得到3株抗除草剂Basta的转基因植株。经PCR及Southern分析,在转基因植株基因组中均检测到了bar基因的整合;经RNA点杂交分析,检测到了bar基因在转基因植株中RNA水平的表达。在转基因植株JY119-2的总计321株T1代自交后代中,有274株表现出除草剂抗性,47株敏感。从该274株抗性植株中随机选取8株进行Southern分析,结果均检测到了bar基因的整合,表明bar基因已遗传到了T1代植株中

     
    << 更多相关文摘    
    图标索引 相关查询

     


     
    CNKI小工具
    在英文学术搜索中查有关bar基因的内容
    在知识搜索中查有关bar基因的内容
    在数字搜索中查有关bar基因的内容
    在概念知识元中查有关bar基因的内容
    在学术趋势中查有关bar基因的内容
     
     

    CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
    版权图标  2008 CNKI-中国知网
    京ICP证040431号 互联网出版许可证 新出网证(京)字008号
    北京市公安局海淀分局 备案号:110 1081725
    版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社