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The venom of Agklstrodon acutus (AAV) (0.5mg/kg) was given Intravenously to anesthetized dogs. It was observed that the AAV caused in the dogs a pro- nounced decrease in platelet counts and plasma fibrinogen levels, a marked prolon- gation of "PT" and KPTT tests, a significantly elevated levels of fibrinogen/fibrin degradation products (FDPs) in scrum, positive 3P test results and the occurrence of glomerular fibrin depositions. These data suggest that acute disseminated ntravascular coagulatiou(DIC) is induce... The venom of Agklstrodon acutus (AAV) (0.5mg/kg) was given Intravenously to anesthetized dogs. It was observed that the AAV caused in the dogs a pro- nounced decrease in platelet counts and plasma fibrinogen levels, a marked prolon- gation of "PT" and KPTT tests, a significantly elevated levels of fibrinogen/fibrin degradation products (FDPs) in scrum, positive 3P test results and the occurrence of glomerular fibrin depositions. These data suggest that acute disseminated ntravascular coagulatiou(DIC) is induce d by the AAV in the dogs, thereby aggravating severe bleedina produced directly by the same venom. In addition, the mechanism for the AAV-mediated acute DIC is discussed preliminarily. 用尖吻蝮蛇毒(Agkistrodon acutus venom,AAV)(0.5mg/kg)静注麻醉狗。观察到AAV对狗引起血小板计数和血浆纤维蛋白原含量明显下降,“PT”和KPTT试验显著延长,血清FDP大大增加,3P试验阳性以及肾小球内呈现纤维蛋白沉积。这些结果表明,AAV对狗引起了急性DIC,因而加重由AAV直接引起的严重出血。此外,对由AAV导致的急性DIC机理作了初步讨论。 The TSV (0.75mg/kg) was given intravenously to anesthetized rabbits. It was observed that the TSV caused a marked prolongation of TT (P<0.01), positive PPP test results and the microemboli in the microvasculature of the lungs, etc. These date suggest the animal model of acute DIC may be induced by TSV in the rabbits.The pathogenesis of DIC induced by TSV is also discussed preliminarily. 用竹叶青蛇毒(Trimeresurus stejnegeri venom,TSV)0.75mg/kg静注麻醉后的家兔,观察到TSV引起TT显著延长(P<0.01),PPP试验阳性以及肺微血管内出现微血栓等变化,提示应用竹叶青蛇毒可建立急性DIC动物模型。文中对竹叶青蛇毒导致的DIC机理作了初步讨论。 Twenty six rabbits were randomized divided into three groups, acute DIC(n=10). anisodamine treatme(n=10) andcontrol(n=6). Acute DIC was induced by intravenous infusion of thrombin (60u/kg) combined with EACA(0.4g/kg) for one hour. Microcirculatory blood flow in kidney. liver, stomach, jejunum, colon and skin weremeasured with Laser-Doppler Flowmeter at 0. 30. 60. 120minutes. The results showed that in DIC group microcirculatory blood flow in above organs we studied were all decreased as compared with that in... Twenty six rabbits were randomized divided into three groups, acute DIC(n=10). anisodamine treatme(n=10) andcontrol(n=6). Acute DIC was induced by intravenous infusion of thrombin (60u/kg) combined with EACA(0.4g/kg) for one hour. Microcirculatory blood flow in kidney. liver, stomach, jejunum, colon and skin weremeasured with Laser-Doppler Flowmeter at 0. 30. 60. 120minutes. The results showed that in DIC group microcirculatory blood flow in above organs we studied were all decreased as compared with that in control at 120 minutes and microcirculatory blood flow changes in skin was positively significantly correlated to these changes in kidney, liver, stomach and jejunum as well as colon. These results suggest that in acute DIC and after treatment with anisodamine. microcirculatory blood flow changes in kidney, liver and gastrointestinal tract could be speculated on that change in skin. 健康大耳白兔26只。用激光多普勒血流计测定皮肤与肾脏、肝脏、胃、空肠及结肠表面微循环血流量,随机分为三组:急性DIC组(n=10)、山莨菪碱治疗组(n=10)和对照组(n=6)。以凝血酶(60u/kg)加氨基乙酸(0.4克/kg)静脉输注1小时复制急性DIC模型,实验30、60、120分钟测定上述脏器表面微循环血流量变化。结果急性DIC组皮肤与肾、肝、胃、空肠及结肠微循环血流量在120分钟均较对照组明显降低,三组动物皮肤微循环血流量的变化与上述内脏表面微循环血流量的变化呈高度正相关。结果提示急性DIC时及山莨菪碱治疗后皮肤微循环血流量的动态变化可间接反映肾、肝、胃肠道微循环血流量的变化。
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