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棉花细胞
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  cotton cells
     cells, resistant Yumian 8 and susceptible Yumian 6, and two types of wilt pathogens (Verticillium dahlium Kle.b), high virulent V44 and low virulent V64. Different resistant cotton cells were induced directly by different virulent pathogens, and inter-induced by different virulent pathogens that had been induced by cotton cells.
     品种作为细胞系,选择高毒的V44和低毒的V64两种大丽轮枝菌(Verticilliumdahlium Kle.b)为黄萎病原菌。 设计不同毒力的病原菌分别直接诱导不同抗性的棉花细胞、不同毒力的病原菌和不同抗性的棉花细胞互相诱导的几种互作模式。
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     (S or R) The PAGE spectrums of POD isozymes from cotton cells showed that (1) all oligosaccharins induced cotton cells to produce new POD isozymes;
     棉花细胞的过氧化物酶 (POD)同工酶结果表明 :(1)所有寡糖素均诱导棉花细胞产生新的过氧化物酶 ;
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     When treated with different concentrations of oligochitosans, oxidative burst and the eliminated enzymes in suspended cotton cells culture could be changed, the oxidative burst maximum appeared in 20~30 minutes, and the maximum changes of SOD and CAT activities happened in 60~90 minutes.
     以不同浓度壳寡糖为激发子 ,均可诱导棉花悬浮细胞的活性氧迸发 ,其峰值出现时间都在 2 0~ 30min左右 ,还可以诱导棉花细胞中活性氧清除酶系活性的变化 ,SOD和CAT活性变化的最大值在处理后 6 0~ 90min左右。
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  cotton cell
     Gus Gene Expression in Transgenic Cotton Cell and Its Correlation with NPTⅡ and Bt Genes
     Gus基因在转基因棉花细胞中的表达及其与NPTⅡ基因、Bt基因表达的相关性
短句来源
     Study on Property and Selection of Saline Tolerance of Cotton Cell (Gossypium hirsutum L. )
     棉花细胞耐盐性筛选研究
短句来源
     Effects of LaCl_3 and TFA on Oligochitosan Induced Cotton Cell Resistance
     氯化镧和三氟乙酸对壳寡糖诱发棉花细胞抗性的影响
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  “棉花细胞”译为未确定词的双语例句
     Cell Engineering and New Germplasm Development in Cotton
     棉花细胞工程及新种质创造
短句来源
     In this study, an attempt is made to account for the observed difference in the susceptibility of Gossypium hirsutum L. (cv. BD-18 and Simian 3, resistant and susceptible to V. dahliae, respectively) to toxin of Verticillium dahliae, by looking for differences in the timing and level of production of NO and H_2O_2, and their effects on the expression of GST gene.
     本实验以棉花抗病品种BD-18和感病品种泗棉三号的悬浮细胞为材料,研究了棉花细胞在黄萎病菌毒素处理后H_2O_2和NO的变化,并用半定量RT-PCR的方法检测毒素、H_2O_2和NO对棉花谷胱苷肽-S-转移酶(GST)基因转录水平的影响。
短句来源
     SOME CHARACTERISTICS OF SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN UOTTON CELL SUSPENSION CULTURE
     棉花细胞悬浮培养胚胎发生和植株再生某些特性的研究
短句来源
     THE PRELIMINARY CYTOMORPHOLOGY OBSERVATION OF THE COTTON INDUCED MALE STERILITY BY FOSAMINE
     调节膦诱导雄性不育棉花细胞形态初步观察
短句来源
     Studies on the Formation of Fibers in Cell Suspension Cultures Derived from Various Cotton (Gossypium hirsutum L.) Explants
     不同来源的棉花细胞在悬浮培养条件下诱导形成纤维的研究
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  cotton cells
The onset and duration of these periods are somewhat different for soybean and cotton cells.
      
One to two weeks following bombardment, embryogenic cotton cells were placed in proliferation medium containing 100 μg/ml hygromycin.
      
β-Galactosidase activity in cultured cotton cells (Gossypium Hirsutum L.): A comparison between cells growing on sucrose and lac
      
Buffer capacity of cotton cells and effects of extracellular pH on growth and somatic embryogenesis in cotton cell suspensions
      
Cotton cells actively adjust medium with initial pH between 3 and 8 to near pH 5.5 in the early culture period.
      
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  cotton cell
Oligosaccharide Inhibits Ethylene Synthesis and Stimulates Somatic Embryogenesis in a Cotton Cell Culture
      
Auxins and cytokinin alone or in the presence of GA3 did not promote cotton cell elongation above the value for the treatment with GA3 alone.
      
A cotton cell suspension culture has been developed that provides unique opportunities for plant biologists to investigate early developmental events regulating cotton fiber properties, plant cell elongation, and cell wall biogenesis.
      
Buffer capacity of cotton cells and effects of extracellular pH on growth and somatic embryogenesis in cotton cell suspensions
      
When an aliquot of cotton cell suspension culture (Gossypium hirsutum L.
      
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β 1, 3 glucanase plays an important role in defense reaction of plants. The hydrolysate of cell walls was prepared from the hyphae of fusarium moniliforme inducing moniformae in cotton seeding. The induction activities of the hydrolysate to β 1, 3 glucanase were detected in cotton leaves and cultured cells. The induction of β 1,3 glucanase and β 1, 3 glucanase was studied in pretreatment cultured cells by salicylic acid. The results show that the hydrolysate of cell wall from fusarium moniliforme induced...

β 1, 3 glucanase plays an important role in defense reaction of plants. The hydrolysate of cell walls was prepared from the hyphae of fusarium moniliforme inducing moniformae in cotton seeding. The induction activities of the hydrolysate to β 1, 3 glucanase were detected in cotton leaves and cultured cells. The induction of β 1,3 glucanase and β 1, 3 glucanase was studied in pretreatment cultured cells by salicylic acid. The results show that the hydrolysate of cell wall from fusarium moniliforme induced β 1,3 glucanase and that calcium was needed during the induction. The pretreatment with salicylic acid promoted the response of enzymes POD and β 1, 3 glucanase in cotton cells. The induced accumulation of the emymes were accelerated by salicylic acid.

从引起棉花红腐病的病原真菌——串珠镰孢菌(Fusariummoniliforme)的菌丝中制备了细胞壁水解产物,测定了其对棉花叶片和棉花组培细胞的β-1,3-葡聚糖酶的诱导能力.结果表明,串珠镰孢菌菌丝细胞壁水解产物可以诱导棉花细胞的β-1,3-葡聚糖酶的活性增加.诱导过程需要钙离子.用水杨酸预先作用棉花组培细胞可以提高细胞对病原菌菌丝细胞壁水解产物的诱导敏感性,使β-1,3-葡聚糖酶和另一类与植物抗病反应有关的酶(过氧化物酶)达到最大活性的时间提前.

The plant cell transformation of cotton cultivars with plasmid containing Gus gene,NPTⅡ gene and Bt gene driven by CaMV 35S promoter was carried out by using Agrobacterium mediaum . The transgenic colli and leaf tissues of the putative transgenic cotton plants were treated with X glucuronide,and the transgenic tissue expressed the turned blue Gus gene.The results of Histochemical Gus assay suggested that the Gus gene was stably transmitted into the next generation cell in callus mitotic cycle. The Gus activity...

The plant cell transformation of cotton cultivars with plasmid containing Gus gene,NPTⅡ gene and Bt gene driven by CaMV 35S promoter was carried out by using Agrobacterium mediaum . The transgenic colli and leaf tissues of the putative transgenic cotton plants were treated with X glucuronide,and the transgenic tissue expressed the turned blue Gus gene.The results of Histochemical Gus assay suggested that the Gus gene was stably transmitted into the next generation cell in callus mitotic cycle. The Gus activity ,NPTⅡ and Bt activity of the progenies(R 1,R 2) of trasgenic cotton plants (R 0) showed the genetic correlation but partly coexpression,the expression of the three transgenes turn out to be heterogeneous.

通过农杆菌介导法将含有 Gus基因、NPT 基因、Bt基因的 Ti质粒转入棉花细胞中 ,用Gus组织化学检测法检测转化愈伤组织、再生棉株 R0 、R1和 R2 代植株组织细胞中的 Gus基因表达的水平 ,并用 NPT 活性速测法和室内生物测虫法检测三个外源基因在 R0 、R1和 R2 代植物植株组织中的表达情况 ,分析三者存在及表达的相互关系。结果表明 ,Gus基因在转化愈伤组织细胞有丝分裂增殖过程中能稳定遗传给后代细胞。转化棉株 R0 、R1和 R2 代 Gus活性、NPT 活性、抗虫性三者有一定的相关性但不完全是共表达的 ,各基因的表达水平是多相的

Employing HPT as a selection marker gene and hygromycin as a selection agent, The GFP gene was transformed into cotton via agrobacterium and regeneration plants were acquired. Transgenic plants were confirmed by southern hybridization, expression of the GFP gene was showed with fluorescence microscope. Using hygromycin as a selection agent in the cotton transformation, the reasonable selection concentration of should be 2.5~10 mg·L 1 if marker gene, such as GFP gene , exists or easy to be examined...

Employing HPT as a selection marker gene and hygromycin as a selection agent, The GFP gene was transformed into cotton via agrobacterium and regeneration plants were acquired. Transgenic plants were confirmed by southern hybridization, expression of the GFP gene was showed with fluorescence microscope. Using hygromycin as a selection agent in the cotton transformation, the reasonable selection concentration of should be 2.5~10 mg·L 1 if marker gene, such as GFP gene , exists or easy to be examined , the start concentration of hygromycin in the medium should be 2.5 mg·L 1 ; If no marker gene exists or the examination is very complicated, the start concentration of hygromycin could be higher as 10 mg·L 1 . So that the higher rate of transformation can be obtained.

以 HPT基因作为筛选标记基因 ,潮霉素作为筛选剂 ,通过农杆菌介导法将 GFP基因导入棉花细胞并得到再生植株。经过 Southern杂交证实外源基因已经整合到棉花基因中 ,荧光显微镜可以观察到绿色荧光 ,说明 GFP基因得到表达。研究确定了潮霉素作为棉花转化的筛选剂在农杆菌介导的转化中适用的浓度范围及筛选方法 ,即棉花转化中潮霉素的筛选浓度范围为 2 .5~ 1 0 mg· L- 1,在有 GFP等一些易于观察和检测的标记基因存在时 ,起始浓度可用较低 ( 2 .5 mg· L- 1)的筛选浓度 ;而在没有标记基因或检测方法较复杂时 ,起始浓度用较高 ( 1 0 mg· L- 1)的筛选浓度

 
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