Bitter peptides were extracted from the hydrolysate of SPI by n-butanol and the molecular weight of the bitter peptides were between 300-1500D. The ratio of the bitter peptides covers 24.1% of the whole soy peptides.
The molecular weight distribution of peptides in Harpodon Nehereus hydrolysates and their bitter peptide fractions were determined by gel permeation chromatography on a Sephadex G-25 super fine column. Results indicated that the molecular weight range of the fraction enriched in bitter peptides was 470-1370 Da.
The molecular weight distribution of peptides in Harpodon Nehereus hydrolysates and their bitter peptide fractions were determined by gel permeation chromatography on a Sephadex G-25 super fine column. Results indicated that the molecular weight range of the fraction enriched in bitter peptides was 470-1370 Da.
Subsequently, the bitter fractions recognized were separated by reversed phase HPLC on LUNA C18 column and confirmed by sensory testing, three peptides with the most bitter flavor(p2, p4, p8) were obtained.
Enzymatic hydrolysis of soybean protein frequently results in bitter taste, and the bitterness in protein hydrolyzate has been a factor that hinders its application for a long time.
Three strains M1,M2,and M3 with high protease yield were isolated from soybean paste by natural fermentation. With three strains,Mucor sp.,Aspergillus niger and Aspergillus terricola,they were cultivated in solid and liquid mediums. The strain M3 was screened for protease-producing and debitterness activities.
Thus, according to our theory, by enzymatic hydrolysis, casein, soyabean-protein and zein form bitter peptides, whereas collagen and muscle protein do not form bitter peptides.
Two bitter peptides, H-Phe-Tyr-Pro-Glu-Leu-Phe-OH (I) and H-Val-Glu-Val-Phe-Ala-Pro-Pro-Phe-OH (II) were isolated from casein, hydrolyzed by α-chymotrypsin.
As could be shown recently for bitter amino acids, a polar (electrophilic) and a hydrophobic group are essential requirements for bitter peptides also.
The results suggest that the main reason for the bitterness of soybean protein hydrolysates prepared by Alcalase treatment are hydrophobic bitter peptides of relative molecular mass less than 1000.
Production of a Bitter Peptide from Casein and its Sensory Characterization.
例句来源
In this research,the protein is extracted by water from de-fatted soybean meal and purified by acidprecipitation In order to control the bitter peptide taste effectivily,double enzymolysis is used for the absorption byabsorbent carbon .The process technique and parameters of soy peptide are presented in this article and can beapplied to large-scale industrial production.
Bitter peptides are extracted from hydrolysate of chicken and its composition of amino acids is analyzed.Then the hydrolysis effect of bitter peptides is studied by using endoenzyme from Lactobacillus and Brewing Yeast,and the change of aminos acids content is analyzed before and after hydrolysis.
The bitter peptides obtained form the soybean protein hydrolyzed by pepsin were applied to Sephadex G-15 column.Three bitter fractions were collected.Their bitter value is 5,3,2.5 respectively and their molecular weight is 868,651,361 estimatde according to the equation:-lgKav =0.005 67M2/3 -0.182 79.The conditions of the column chromatography are as followings:the column,1.5 cm×76 cm;loading volume,1.5 mL;flow rate,0.5 mL/min;elution buffer,0.05 mol/L NaH2PO4-0.15 mol/L NaCl.