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   定量pcr 在 消化系统疾病 分类中 的翻译结果: 查询用时:0.023秒
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定量pcr     
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  quantitative pcr
    Methods 351 positive serum of HBsAg,anti-HBe,anti-HBc of hepatitis B and 30 healthy man wrer collected. Pre-S1 antigen and HBV markets were detected with ELISA and HBV-DNA with fluorescent quantitative PCR(FQ-PCR) respectively.
    方法:收集351例乙型肝炎小三阳患者血清和30例正常体检者血清,用ELISA方法检测前S1抗原,用荧光定量PCR方法定量检测HBV-DNA。
短句来源
    Tissue HBV DNA levels were determined by realtime fluorescence quantitative PCR.
    荧光实时定量PCR检测肝组织HBV DNA含量;
短句来源
    METHODS: Th1 cytokine (IL-2, IFN-γ, TNF-α) in supernatant and HCV RNA in serum were assayed by ELISA and fluorescent quantitative PCR respectively after PBMCs in patients with chronic HCV infection were cultured in vitro for 72 h. HCV RNA genotype was detected by RT-PCR.
    方法:体外培养慢性HCV感染患者PBMC72h后,ELISA检测培养上清Th1类细胞因子(IL-2,IFN-γ,TNF-α),荧光定量PCR检测患者血清HCV RNA含量,RT-PCR检测HCV RNA亚型.
短句来源
    The mRNA levels of tissue factor(TF),plasminogen activator inhibitor(PAI-1),monocyte chemoattractant protein(MCP-1),Gro-1,IL-6 and ICAM-1 were measured by quantitative PCR.
    并利用定量PCR的方法,检测胰腺组织中炎症相关因子组织因子(TF)、纤维蛋白溶酶原激活因子抑制因子(PAI-1)、单核细胞趋化吸引蛋白1(MCP-1)、Gro-1、IL-6和细胞间黏附分子-1(ICAM-1)mRNA的表达。
短句来源
    Grading quantitative PCR to measure serum HBV DNA levels
    分级定量PCR检测血清HBV DNA
短句来源
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  quantity pcr
    Real-time quantity PCR was applied to detect the contents of introcellular HBV mRNA and extracellular HBV DNA.
    荧光定量PCR分析PTW-6对细胞内HBV mRNA和上清液中HBV DNA含量的影响。
短句来源
    HBVDNA in serum were determined by fluorecence quantity PCR(FQ-PCR) . The patients were divided into 4 groups(HBVDNA< 103 copies/ml, 103 - 105 copies/ml, 105 - 107 copies/ml, >107 copies/ml).
    荧光定量PCR检测患者血清 DNA含量,并分成 HBVDNA小于103拷贝/ml、103~105拷贝/ml、105~107拷贝/ml、大于 107拷贝/ml4组。
短句来源
    Serum level of HBV-DNA was determined by real-time fluorescence quantity PCR. Serum PreS1 and HBeAg were determined by ELISA.
    方法30例健康对照者血清和272例未经抗病毒治疗的乙型肝炎患者血清,同时采用实时荧光定量PCR法测定HBV-DNA载量、ELISA法检测PreS1和HBeAg。
短句来源
  quantitative fluorescence pcr
    Objective To investigate the relation ship between serum cytokines and hepatitis B Methods The levels of serum cytokines,ALT,TBil and HBV DNA were measured in 96 patients with hepatitis B and 22 normal controls by ELISA,colorimetry and quantitative fluorescence PCR technique Results Except latent hepatitis,the level of serum TNF α,IL 6,IL 8,IL 12 and IL 18 in different type of hepatitis B were significantly higher than those of controls( P <0 001);
    方法 运用ELISA法、比色法及荧光定量PCR技术检测不同类型乙型肝炎患者血清细胞因子、ALT、TBil及HBV DNA水平 ,观察患者治疗前后细胞因子变化及其与预后的关系。 结果 除隐匿性肝炎外 ,急性、慢性及重型肝炎患者血清TNF α、IL 6、IL 8、IL 12、IL 18的水平均显著高于对照组(P <0 0 1) ,升高程度与病情相关 ;
短句来源
    Objective To study the relationship between levels of hyaluronic acid(HA),type Ⅳ collagen(ⅣC),Laminin (LN) in sera and hepatic fibrosis in patients with chronic hepatitis B.Methods 252 sera from patients with chronic hepatitis B were collected to measure hyaluronate,type Ⅳ collagen,laminin by radioimmunoassay,and sera HBV-DNA were also tested by quantitative fluorescence PCR respectively.
    目的 研究血清肝纤维化指标透明质酸 (HA)、Ⅳ型胶原 (ⅣC)及层粘蛋白 (LN)与慢性乙型肝炎肝纤维化的关系。 方法 对 2 5 2例慢性乙型肝炎患者用放射免疫法检测血清HA、ⅣC、LN水平及定量PCR检测血清HBV -DNA。
短句来源
    Objective:To investigate the relationship between serum cytokines and hepatitis B.Methods:The levels of serum cytokines, ALT, TBil and HBV-DNA were measured in 96 patients with hepatitis B and 22 normal controls by ELISA, colorimetry and quantitative fluorescence PCR technique.
    目的 探讨血清细胞因子水平与乙型肝炎病变的关系。 方法 运用ELISA法、比色法及荧光定量PCR技术检测不同类型乙型肝炎患者血清细胞因子、ALT、TBil及IBV -DNA水平 ,观察患者治疗前后细胞因子变化及其与预后的关系。
短句来源
  “定量pcr”译为未确定词的双语例句
    FQ-PCR technique was used to study the inhibitory effects of HOGS on the content of HBV-DNA in HepG2-2.2.15 cells.
    采用荧光探针定量PCR检测HOGS对HepG2-2.2.15细胞HBV-DNA复制的影响。
短句来源
    Methods The serum YMDD mutation,HBV DNA,ALT and HbeAg levels of 60 patients on lamivudine therapy were detected by FQ-PCR,ELISA,and rate method,respectively.
    方法采用实时荧光定量PCR、ELISA及速率法分别检测60例乙肝患者经拉米呋啶治疗后其血清YMDD、HBV DNA、乙肝标志物及ALT的变化情况。
短句来源
    The expression of Pokemon in all tissues was detected by real-time PCR.
    用real-time PCR(实时定量PCR,RT- PCR)法检测Pokemon蛋白mRNA在这些组织中的表达。
短句来源
    METHOD:after cultured 72 hour in vitro,cytokines (IL-2、IL-4、IL-10、IFN-γ、TNF-α) in supernatant secreted by PBMCs were detected by ELISA. HCV RNA in serum were assayed by fluorescent quantitation PCR.
    方法:体外培养慢性HCV感染患者PBMCs72小时后,ELISA检测培养上清细胞因子(IL-2、IL-4、IL-10、IFN-γ、TNF-α)含量,荧光定量PCR检测患者血清HCV RNA含量。
短句来源
    Furthermore,the mRNA of TF,PAI,MCAP,ICAM-1 and IL-6 in pancreata were significantly decreased in Egr-1 knockout mice.
    定量PCR检测结果表明,Egr-1基因剔除组,胰腺组织中TF、PAI-1,以及MCP-1、ICAM-1和IL-6 mRNA的表达,明显少于野生型组。
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  quantitative pcr
Quantitative PCR showed that 5α-dihydrotestosterone increases the level of the PTGF-β mRNA.
      
annua seedlings cultured in vitro by semi-quantitative PCR (SQ-PCR).
      
The real-time fluorescent quantitative PCR (RFQ-PCR) assay of A.
      
Gene and protein expression was analyzed by real-time quantitative PCR, immunohistochemistry and Western blotting, respectively.
      
Quantitative PCR and hydridization were used to determine the effect of inhibition of HCV by ribozymes.
      
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The correlation between hepatitis B virus(HBV)and hepatocellular carcinoma(HCC)has been confirmed by WHO since 1983.The viewpoint on the hepatitis B virus activity status is controversial after HCC established in patients and the effect surgical interventions does on hepatitis virus in this stage is unclear.We investigated 111 HCC patients with or without positive HBV markers the status of HBV activity by using PCR methods.The results show that replication process of hepatitis B virus is still strongly continuing...

The correlation between hepatitis B virus(HBV)and hepatocellular carcinoma(HCC)has been confirmed by WHO since 1983.The viewpoint on the hepatitis B virus activity status is controversial after HCC established in patients and the effect surgical interventions does on hepatitis virus in this stage is unclear.We investigated 111 HCC patients with or without positive HBV markers the status of HBV activity by using PCR methods.The results show that replication process of hepatitis B virus is still strongly continuing in some patients and surgical interventions may result in promoting the process of virus replication.It is Suggested that we should pay more attention to the virus as well as to HCC in clinical practice.

对收治的97例肝癌病人(其中31例经手术治疗)采用定性PCR方法,并进一步采用定量PCR方法对14例乙肝阳性肝癌病人外科处理前后配对,研究了病毒活动状态及外科干预对病毒活动变化的影响。结果显示:肝癌阶段乙肝病毒仍然在一大部分病人体内进行着活跃的复制活动;外科干预更加激发乙肝病毒的复制过程。建议在肝癌外科干预的同时应当防范乙肝的活动。

A 238 bp HEV cDNA was amplified from plasma of a patient positive to anti HEV IgM. It was inserted into the NcoⅠ site of HCV 5'NCR sequence cloned into the vector pSP72. A plasmid pSnc e was recombinated that can transcribe a mutated HCV RNA serving as the competitive RNA template for quantitative RT PCR assay. The pSnc e was determined by PCR and restrictive site analysis.

将克隆的HCV5’NCR序列反向插入pSP72的EcoRV切点,构建一种能转录HCV-RNA的质粒pSnc-2。以RT-PCR从抗HEVIgM阳性病人血浆中扩增一段238bp的HCV-cDNA,反向插入pSnc-2中克隆的5’NCR序列的NcoⅠ切点,构建插入突变型HCV-cDNA重组体pSnc-e。应用限制性酶切和PCR对这两种重组质粒进行了鉴定,为HCV-RNA的定量PCR提供有用的内参照模板。

Detection of the level of sIL-2R and the content of HCV-RNA in sera on 123 patients of hepatitis C by using sandwich ELISA and quantitutive PCR for clinical significance of slL-2R in all sorts hepatitis C and relationship between its change. The content of HCV shows that the level of slL-2R in serum of all sorts hepatitis C is higher than that health group (P<0. 01 )and the ctrrosis livers is highest. The change of leveL of sIL-2R is directly related to the content of HCV,and on the contrary in cirrhosis liver,it...

Detection of the level of sIL-2R and the content of HCV-RNA in sera on 123 patients of hepatitis C by using sandwich ELISA and quantitutive PCR for clinical significance of slL-2R in all sorts hepatitis C and relationship between its change. The content of HCV shows that the level of slL-2R in serum of all sorts hepatitis C is higher than that health group (P<0. 01 )and the ctrrosis livers is highest. The change of leveL of sIL-2R is directly related to the content of HCV,and on the contrary in cirrhosis liver,it shows that sIL-2R has mutual immunifaction and its level is related to immunity of body and hurt in liver.

为探讨sIL-2R在各型丙肝中的临床意义,以及其水平变化与HCV含量之间的关系,本文采用双抗体夹心法和定量PCR技术,对123例各型丙肝血清sIL-2R水平、HCV-RNA含量进行检测。结果显示,各型丙肝血清sIL-2R水平与正常组比较均有显著性升高(P<0.01),尤以肝硬化组升高最为明显。并且其水平变化在急性和慢性肝炎时与HCV含量呈正相关,在肝硬化时呈明显负相关。提示,sIL-2R具有双向免疫调节作用,其水平变化在一定程度上反映了机体的免疫状态和肝脏的病理损伤程度。

 
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