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A study of the efficiency of different methods to transfer a reporter gene to chicken embryonic cells
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The methods of transfection of a plasmid with a reporter gene involving DNA injection into chicken embryonic cells were studied.
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The parameters of the efficient transfection of chicken blastodermal cells with a foreign gene have been determined (20-24 and up to 40% in culture and embryos, respectively).
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A high efficiency of transfection of primordial germ cells isolated from the gonads has been obtained after DNA injection into the dorsal aorta of 2.5-day-old chicken embryos.
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Effects of Osmolytes on Unfolding of Chicken Liver Fatty Acid Synthase
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The proliferating multipotent cells are preserved in the ciliary-terminal zone of the retina of amphibians, fish, and chickens during their entire life.
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Its uniqueness suggests that yolk DNA may play a crucial role in early development of chickens.
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In this experiment, F2 chickens derived from Broilers crossing to Silky were used to study the effect of extracellular fatty acid binding protein (EX-FABP) gene on abdominal fat accumulation.
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Therefore, Silky chickens were selected for production of heterozygotes to confirm whether IGF2 locus was imprinting.
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IGF2 gene was expressed bialleleically in 1-day-old neonatal liver and 90-day-old liver, kidney, heart, and muscle of both heterozygote AB and BA chickens.
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The third section covers the data on liver differentiation, the formation of the glycogen organelle with embedded enzymes of glycogen synthesis, and the degradation of chick and rat embryos during development.
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Kinetics of β1-Adrenergic Reaction of the Chick Embryo Amnion
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Dose-dependent curves of the inhibitory β1-adrenergic reaction of the chick embryo amnion were analyzed on the basis of different mathematical models.
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A tenfold increase in chlororespiration during dark incubation of Chlorella perynoidosa Chick CALU-175 at high temperature doubled the initial chlorophyll fluorescence yield (F0).
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Extracellular Carbohydrates and Polysaccharides of the Alga Chlorella pyrenoidosa Chick S-39
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Egg Fertility in Domestic Fowl Gallus gallus domesticusas a Function of the Yolk Surface Area
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Development of the mitochondrial apparatus and blood supply of skeletal muscle fibers during ontogenesis of domestic fowl
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FILIAL IMPRINTING AND LEADERSHIP AMONG CHICKS IN FAMILY INTEGRATION OF THE DOMESTIC FOWL
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Tracheae obtained from the domestic fowl were incubated with a pronase/EDTA mixture to dissociate the epithelial cells.
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We concluded that egg transformation is a rare event in domestic fowl and further research for use of this technique in commercial poultry breeding is needed.
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