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   酯酶基因 在 生物学 分类中 的翻译结果: 查询用时:0.471秒
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  esterase gene
    Expression of esterase gene from Ralstonia eutropha CH34 in Escherichia coli and properties of recombinant eaterase EstA
    Ralstonia eutropha CH34酯酶基因在大肠杆菌中的表达及酶学特性
短句来源
    STUDIES OF ESTERASE GENE AMPLIFICATION IN ORGANOPHOSPHATE RESISTANT CULEX PIPIENS COMPLEX FROM AMERICA AND CHINA
    中美有机磷杀虫药剂抗性库蚊复合种群酯酶基因扩增的研究(英文)
短句来源
    Automated programming of degenerate primers and the cloning of the diamondback esterase gene.
    程序化设计简并引物与克隆小菜蛾酯酶基因
短句来源
    The results showed that OP resistance and amplification of esterase were both lost when strain was not homozygous for the presence of the amplified gene,due probably to biological disadvantage of esterase gene.
    研究结果表明,当抗性种群中混有非酯酶基因扩增个体时,其抗性水平逐渐下降,酯酶扩增的概率逐渐减少,这可能与抗性基因在生物学上的不适应性有关;
短句来源
    In homozygous strain,esterase remained amplified and OP resistance was still stable,indicated that copies of esterase gene were not lost in offspring of this strain.
    在均一的抗性酯酶基因种群中,抗性水平始终较稳定,酯酶的活性也一直较高,这表明在均一的抗性酯酶基因种群的遗传中,酯酶等位基因拷贝几乎是不会丢失的。
短句来源
更多       
  esterase gene
    Expression of esterase gene from Ralstonia eutropha CH34 in Escherichia coli and properties of recombinant eaterase EstA
    Ralstonia eutropha CH34酯酶基因在大肠杆菌中的表达及酶学特性
短句来源
    STUDIES OF ESTERASE GENE AMPLIFICATION IN ORGANOPHOSPHATE RESISTANT CULEX PIPIENS COMPLEX FROM AMERICA AND CHINA
    中美有机磷杀虫药剂抗性库蚊复合种群酯酶基因扩增的研究(英文)
短句来源
    Automated programming of degenerate primers and the cloning of the diamondback esterase gene.
    程序化设计简并引物与克隆小菜蛾酯酶基因
短句来源
    The results showed that OP resistance and amplification of esterase were both lost when strain was not homozygous for the presence of the amplified gene,due probably to biological disadvantage of esterase gene.
    研究结果表明,当抗性种群中混有非酯酶基因扩增个体时,其抗性水平逐渐下降,酯酶扩增的概率逐渐减少,这可能与抗性基因在生物学上的不适应性有关;
短句来源
    In homozygous strain,esterase remained amplified and OP resistance was still stable,indicated that copies of esterase gene were not lost in offspring of this strain.
    在均一的抗性酯酶基因种群中,抗性水平始终较稳定,酯酶的活性也一直较高,这表明在均一的抗性酯酶基因种群的遗传中,酯酶等位基因拷贝几乎是不会丢失的。
短句来源
更多       
  lipase gene
    Breeding of Lipoprotein Lipase Gene Knockout Mice
    脂蛋白酯酶基因敲除小鼠的繁育
短句来源
  esterase gene
    Expression of esterase gene from Ralstonia eutropha CH34 in Escherichia coli and properties of recombinant eaterase EstA
    Ralstonia eutropha CH34酯酶基因在大肠杆菌中的表达及酶学特性
短句来源
    STUDIES OF ESTERASE GENE AMPLIFICATION IN ORGANOPHOSPHATE RESISTANT CULEX PIPIENS COMPLEX FROM AMERICA AND CHINA
    中美有机磷杀虫药剂抗性库蚊复合种群酯酶基因扩增的研究(英文)
短句来源
    Automated programming of degenerate primers and the cloning of the diamondback esterase gene.
    程序化设计简并引物与克隆小菜蛾酯酶基因
短句来源
    The results showed that OP resistance and amplification of esterase were both lost when strain was not homozygous for the presence of the amplified gene,due probably to biological disadvantage of esterase gene.
    研究结果表明,当抗性种群中混有非酯酶基因扩增个体时,其抗性水平逐渐下降,酯酶扩增的概率逐渐减少,这可能与抗性基因在生物学上的不适应性有关;
短句来源
    In homozygous strain,esterase remained amplified and OP resistance was still stable,indicated that copies of esterase gene were not lost in offspring of this strain.
    在均一的抗性酯酶基因种群中,抗性水平始终较稳定,酯酶的活性也一直较高,这表明在均一的抗性酯酶基因种群的遗传中,酯酶等位基因拷贝几乎是不会丢失的。
短句来源
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Pulsed-field gel electrophoresis analysis of Beijing and Californian populations of Culex pipiens complex mosquitoes revealed that the enzyme Mlul produced a band of 49kb. This probably is one whole amplification unit, the largest fragment found so far in the mosquitoes. The method for the preparation of large DNA molecules was described and the electrophoretic condition for the separation of large DNA molecules is discussed.

用脉冲电场凝胶电泳技术,从北京和美国加州的有机磷杀虫药剂抗性库蚊复合品系中分离出一条49kb的酯酶基因扩增片段。该片段可能是一个完整的扩增单元,也是迄今分离出的最大的一条昆虫酯酶基因扩增片段,扩增基因结构的研究正在进行之中。本文还重点讨论了DNA大片段的制备方法和脉冲电场电泳分离技术。

Organophosphate insecticide (OP) resistance in mosquitoes of Culex pipiens complex has been shown to be correlated with the increase in esterase activity resulting from overproduction of the esterase due to amplification of the corresponding structural gene. Several esterase B loci have been identified with Southern hybridization and restriction fragment length polymorphism (RFLP). In this paper, esterase B loci in four OP resistant strains of Culex pipiens pallens were identified with the restriction...

Organophosphate insecticide (OP) resistance in mosquitoes of Culex pipiens complex has been shown to be correlated with the increase in esterase activity resulting from overproduction of the esterase due to amplification of the corresponding structural gene. Several esterase B loci have been identified with Southern hybridization and restriction fragment length polymorphism (RFLP). In this paper, esterase B loci in four OP resistant strains of Culex pipiens pallens were identified with the restriction analysis of PCR fragments. The results indicate that various esterase loci have different selective advantages under different OP pressures. B 1 gene was amplified in temephos resistant strain, and B 2 gene was amplified in trichlorphon and chlorpyrifos resistant strains. The hybridzygote of B 1 and B 2 genes was detected in the malathion resistant strain. Different natural populations of C.pipiens pallens have different probability of B gene distribution. These results are discussed in relation to the selective advantage of esterase genes to OP pressure and mosquito control strategies.

酯酶基因扩增所产生的酯酶活性升高是库蚊 Culex pipiens 对有机磷杀虫剂抗性的主要机理之一。采用分子杂交技术和限制性酶切片段长度多态性 ( R F L P) 分析, 已鉴定出多种酯酶等位基因类型。该文通过酯酶基因特异性片段的 P C R 扩增及扩增片段的酶切片段分析, 对淡色库蚊 Culex pipiens pallens 四种有机磷抗性品系的酯酶等位基因进行分型, 并测定分析自然种群中不同酶型的频率分布。研究结果表明: P C R 分型方法具有快速、准确的特点。不同的有机磷杀虫剂对酯酶等位基因具有明显的选择作用。双硫磷品系为 B1 型; 毒死蜱和敌百虫品系为 B2 型; 马拉硫磷品系为 B1 型和 B1/ B2 杂合型。不同地区采集的种群表现出不同的酶型频率分布。该文就杀虫剂对酯酶等位基因选择作用及自然种群的酶型频率分布进行了讨论

Resistance to chlorpyrifos(OP insecticides)in Culex pipiens pallens was caused by single genes coding for increased OP detoxification by esterase B 2.Here,variation of OP resistance due to amplification of esterase B 2 was investigated in unselected strains.The results showed that OP resistance and amplification of esterase were both lost when strain was not homozygous for the presence of the amplified gene,due probably to biological disadvantage of esterase gene.In homozygous strain,esterase remained...

Resistance to chlorpyrifos(OP insecticides)in Culex pipiens pallens was caused by single genes coding for increased OP detoxification by esterase B 2.Here,variation of OP resistance due to amplification of esterase B 2 was investigated in unselected strains.The results showed that OP resistance and amplification of esterase were both lost when strain was not homozygous for the presence of the amplified gene,due probably to biological disadvantage of esterase gene.In homozygous strain,esterase remained amplified and OP resistance was still stable,indicated that copies of esterase gene were not lost in offspring of this strain.The evolution of OP resistance in mosquitoes is discussed.

淡色库蚊对毒死蜱抗性品系表现为酯酶B2基因扩增。本文对由酯酶B2基因扩增所导致的OP抗性在无杀虫剂压力下的变异性进行了研究。研究结果表明,当抗性种群中混有非酯酶基因扩增个体时,其抗性水平逐渐下降,酯酶扩增的概率逐渐减少,这可能与抗性基因在生物学上的不适应性有关;在均一的抗性酯酶基因种群中,抗性水平始终较稳定,酯酶的活性也一直较高,这表明在均一的抗性酯酶基因种群的遗传中,酯酶等位基因拷贝几乎是不会丢失的。根据研究结果,对蚊虫有机磷杀虫剂抗性的演变进行讨论

 
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