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   酯酶基因 在 农业基础科学 分类中 的翻译结果: 查询用时:0.781秒
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酯酶基因
相关语句
  esterase gene
    Expression of esterase gene from Ralstonia eutropha CH34 in Escherichia coli and properties of recombinant eaterase EstA
    Ralstonia eutropha CH34酯酶基因在大肠杆菌中的表达及酶学特性
短句来源
    Automated programming of degenerate primers and the cloning of the diamondback esterase gene.
    程序化设计简并引物与克隆小菜蛾酯酶基因
短句来源
    At the molecular biology level, esterase gene amplification is the main reason for the insecticide resistance of Myzus persicae.
    在分子水平上 ,桃蚜的抗性机制主要是酯酶基因的扩增。
短句来源
    The resistance mechanisms of homopteran pests to insecticides included metabolic resistance and target resistance, and the former was mainly involved in esterase gene amplification.
    同翅目害虫的抗性机制主要包括代谢抗性和靶标抗性 ,其中前者在分子水平上多涉及酯酶基因扩增。
短句来源
  “酯酶基因”译为未确定词的双语例句
    The inheritance of the amplified esterase genes has also been studied.
    同时对这些扩增的酯酶基因的遗传方式也有一些研究。
短句来源
    The esterases are attributed to closely linked loci named A and B, and overproduction of all esterases B is due to gene amplification.
    酯酶基因位于紧密连锁的A和B座位上。 现已知所有酯酶B的过量产牛都是基因扩增的结果。
短句来源
    Similarity alignment showed that the products of the cloned cDNA were very similar to those of insect esterases.
    经blastx检索genebank,发现此cDNA产物与其它昆虫抗性酯酶基因有高度的相似性。
短句来源
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  esterase gene
The product of αE7 gene, which is a member of α-esterase gene cluster, is probably playing a role in detoxyfication of the xenobiotic esters.
      
Thermostable esterase gene was cloned (Est-AF) from extremophilic microorganisms, Archaeoglobus fulgidus DSM 4304.
      
Genetic control of the other spikelet traits involved factors with large effects which are located in the region of linkage group 6 close to al6 and to an esterase gene, Esterase-E.
      
A genomic DNA library of strain 643A was introduced into Escherichiacoli TOP10F', and screening on tributyrin-containing agar plates led to the isolation of esterase gene.
      
The esterase gene (estA, 621 bp) encoded a protein (EstA) of 207 amino acid residues with molecular mass of 23,036 Da.
      
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in Culex pipiens, overproduction of nonspecific esterases is a commonmechanism of resistance to organol,hosphate insecticides. The esterases are attributed to closely linked loci named A and B, and overproduction of all esterases B is due to gene amplification. In order to determine whether the esterase Bl identified by electrophoretic studies in Culex pip lens mosquitoes from different countries is over-produced due to the amplification of the same DNA haplotype, the amplified region encompassing the structural...

in Culex pipiens, overproduction of nonspecific esterases is a commonmechanism of resistance to organol,hosphate insecticides. The esterases are attributed to closely linked loci named A and B, and overproduction of all esterases B is due to gene amplification. In order to determine whether the esterase Bl identified by electrophoretic studies in Culex pip lens mosquitoes from different countries is over-produced due to the amplification of the same DNA haplotype, the amplified region encompassing the structural esterase Bl gene was characterized by restriction mapping and RFLP. Thesame amplified haplotype was found in mosquitoes with an esterase Bl protein,independently of their geographical origin: French Guyana, Venezuela, Puerto Rico,California and Chuna. Large variations in amplification levels were observed. In addition to the highLly amplified 2.Ikb fragment, EcoRI digestion led to two other restriction fragments of low amplification in mosqintoes from the Americas but not from China. It is concluded that BI amplification has a unique origin, either in America or in Asia, and has subsequently spread on a large scale by migration. Thismigration is more limited than that of A2-B2 esterases, since Bl is confined to the Americas, Carribean and part of China, whereas the A2-B2 distribution now includes the Americas, Carribean, Asia, Africa, Pacific islands and Europe.

在库蚊Culexpipiens品系中,非专一性酯酶的过量产牛是对有机磷杀虫药剂抗性的普遍机理。酯酶基因位于紧密连锁的A和B座位上。现已知所有酯酶B的过量产牛都是基因扩增的结果。为了确定不同国家库蚊品系的酯酶B1的过量产生是否都是相同DNA单基因型扩增的结果,我们构建了酯酶B1结构基因扩增区的限制性内切酶酶切图谱,分析了限制性酶切片段长度多态性(RFLP)。研究发现不同地理位置的酯酶B1库蚊,如法属圭亚那、委内瑞拉、波多黎各岛、美国加利福尼亚和中国北京,都有着相同的单基因扩增,但在扩增水平上有较大的差异。我们认为无论在美洲或亚洲,凡是酯酶B1扩增的库蚊都为同一个起源,之后经迁移而传播到各地;同时发现酯酶B1扩增的库蚊与酯酶A2-B2扩增的库蚊相比,其迁移有一定的局限性;并巨酯酶B1扩增的库蚊仅仅限于美国、加勒比和中国的一些地区,而酯酶A2-B2扩增的库蚊则广泛地分布于美国、加勒比、亚洲、非洲、太平洋各岛及欧洲等地。

This paper reviews the recent researching development of Myzus persicae from the biology and ecology , esterase mechanism and molecular biology mechanism et. three aspects. The adapting ability of different color-forms vary widely. The resistance of green peach aphids to insecticides results mainly from the enhance the levels of the E4 carboxylesterase. Besides ,the marked insensitivity of acetylcholinesterase to insecticide enhanced the resistance of Myzus Persicae(Sulzer) to carbamate. At the molecular biology...

This paper reviews the recent researching development of Myzus persicae from the biology and ecology , esterase mechanism and molecular biology mechanism et. three aspects. The adapting ability of different color-forms vary widely. The resistance of green peach aphids to insecticides results mainly from the enhance the levels of the E4 carboxylesterase. Besides ,the marked insensitivity of acetylcholinesterase to insecticide enhanced the resistance of Myzus Persicae(Sulzer) to carbamate. At the molecular biology level, esterase gene amplification is the main reason for the insecticide resistance of Myzus persicae. The inheritance of the amplified esterase genes has also been studied.

本文从桃蚜的生物生态学、抗药性的酶学机理和分子生物学机理三个方面对桃蚜的最近的研究进展作了综述。不同体色的桃蚜对环境的适应能力不同。桃蚜的抗药性主要是由羧酸酯酶 E4扩增引起的。但是乙酰胆碱酯酶的不敏感性也增加了桃蚜对氨基甲酸酯类农药的抗性。在分子水平上 ,桃蚜的抗性机制主要是酯酶基因的扩增。同时对这些扩增的酯酶基因的遗传方式也有一些研究。

It is a normal strategy to clone protein family cDNA using the degeneracy of the code to design PCR primers from protein multiple\|sequence alignments. With the tools of blastp, blockmaker, CodeHop, SwissProt,and SpTrEMBL, degenerate primers were designed for cloning of the insect esterase cDNA. Using one pair of primers, a partial cDNA was obtained from the diamondback moth by RT\|PCR. Similarity alignment showed that the products of the cloned cDNA were very similar to those of insect esterases. The results...

It is a normal strategy to clone protein family cDNA using the degeneracy of the code to design PCR primers from protein multiple\|sequence alignments. With the tools of blastp, blockmaker, CodeHop, SwissProt,and SpTrEMBL, degenerate primers were designed for cloning of the insect esterase cDNA. Using one pair of primers, a partial cDNA was obtained from the diamondback moth by RT\|PCR. Similarity alignment showed that the products of the cloned cDNA were very similar to those of insect esterases. The results indicated that these degenerate primers designed by this software could be used to obtaining specific RT\|PCR product.

利用遗传密码简并性 ,针对特定的氨基酸序列设计简并引物 ,是克隆蛋白质家族cDNA的常规方法。文章介绍了利用blastp ,blockmaker,CodeHop ,SwissProt,SpTrEMBL等网络工具及数据库设计昆虫抗性酯酶的简并引物。用这对引物从抗有机磷杀虫剂的小菜蛾Plutellaxylostella中克隆了 1段cDNA。经blastx检索genebank,发现此cDNA产物与其它昆虫抗性酯酶基因有高度的相似性。研究表明 ,程序化设计的简并引物可信性强 ,阳性率高 ,能迅速得到满意结果

 
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