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重组卡介苗
相关语句
  recombinant bcg
    INFECTION OF DENDRITIC CELLS BY RECOMBINANT BCG
    重组卡介苗感染树突细胞的研究
短句来源
    Construction, Expression and Identification of a Recombinant BCG Vaccine Encoding Human Mycobacterium tuberculosis Heat Shock Protein 65
    人结核杆菌热休克蛋白65重组卡介苗疫苗的构建、表达及鉴定
短句来源
    Screening and construction of recombinant BCG strains expressing the Ag85B-ESAT6 fusion protein
    分泌表达Ag85B与ESAT6融合蛋白的重组卡介苗菌株的筛选
短句来源
    Construction of A Recombinant BCG Vaccine Expressing the Secretory Form Fusion Protein 85B-ESAT-6
    分泌性表达融合蛋白85B-ESAT-6重组卡介苗的构建和表达
短句来源
    Objective To screen and construct recombinant BCG strains which express the Ag85B-ESAT6 fusion protein.
    目的筛选获得分泌表达Ag85B与ESAT6融合蛋白的重组卡介苗(BCG)菌株。
短句来源
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  “重组卡介苗”译为未确定词的双语例句
    Kinetics of rBCG_initiated T Cell Immune Responses Monitored by ELISPOT Assay
    应用ELISPOT试验测定重组卡介苗诱导的T细胞应答
短句来源
    Identification of T cell epitopes of MalE protein expressed by recombinant Mycobacterium bovis BCG
    重组卡介苗表达的MalE蛋白T细胞表位的鉴定
短句来源
    The mean time to death and death rate in 2 month et al were used to valued the protection of BCG recombinant vaccines. Results: The gene of esat6 mpt64 ag85a ag85b were amplified by PCR. pYUB295 and PMD31 recombinants of these genes were constructed through recombinant technology and identified them by PCR enzyme digestion and DNA sequencing.
    方法:通过基因工程重组技术将结核分枝杆菌保护性抗原ESAT6、MPT64、Ag85A、Ag85B的编码基因分别与穿梭质粒载体PMD31、pYUB295重组,通过电穿孔技术导入卡介苗中,应用PCR扩增、PAGE电泳鉴定重组卡介苗
短句来源
    In order to determine the characteristics of T cell immune responses induced by recombinant Mycobacterium bovis BCG(rBCG)expressing MalE protein of Escherichia coli (rBCG·MalE),the splenocytes from immunized mice were treated to deplete CD4 + or CD8 + T cells by an immunomagnetic selection,then these cells were used to measure the IFN_γ of IL_2 responses to MalE protein or its peptides in enzyme linked immunospot (ELISPOT)assay.
    应用免疫磁性分离技术去除免疫小鼠脾脏细胞中CD4+或CD8+T细胞后 ,在酶联免疫斑点试验中证实表达大肠杆菌MalE蛋白的重组卡介苗 (rBCG·MalE)诱导的T细胞应答是CD4+T细胞依赖的。
短句来源
    The resulting recombinant expression plasmid pBCG3000-85B-ESAT-6 was then transformed by electroporation into BCG, and induced by heating.
    用电穿孔法将pBCG3000-85B-ESAT-6质粒转化BCG细胞,得到重组卡介苗
短句来源
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  recombinant bcg
Therefore, current studies aim at developing recombinant BCG (rBCG) strains secreting Th1-like cytokines to improve the effectiveness of the therapy.
      
Peripheral blood monocytes (PBMC) were stimulated by recombinant BCG and transformed into bacilli Calmette-Guerin activated killer (BAK) cells, and the effect of anticancer BAK cells was studied.
      
The result of MTT showed that the proliferation of PBMC in the recombinant BCG group was more powerful than in the other two groups (P >amp;lt; 0.05).
      
The result of LDH release assay showed that the antitumor activity of BAK cells stimulated by Recombinant BCG was the highest in all groups.
      
We conclude that the recombinant BCG can activate more PBMCs to anti-bladder cancer in vitro than wild-type BCG does.
      
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This study was intended to produce a new living vaccine against leptospirosis using BCG as vector. Leptospiral outer envelop antigen gene OmpL1 was amplified from the genome of pathogenic leptopira serova Lai 017 by PCR, and cloned in E.coliBCG shuttle plasmid pY6002. Recombinant plasmids were isolated by dot blotting with Digoxigeninlabeled OmpL1 gene. After transforming the recombinant plasmids in BCG (Shanghai strain) by electroporation, the genomic DNA of all 21 transformants were prepared and hybridized...

This study was intended to produce a new living vaccine against leptospirosis using BCG as vector. Leptospiral outer envelop antigen gene OmpL1 was amplified from the genome of pathogenic leptopira serova Lai 017 by PCR, and cloned in E.coliBCG shuttle plasmid pY6002. Recombinant plasmids were isolated by dot blotting with Digoxigeninlabeled OmpL1 gene. After transforming the recombinant plasmids in BCG (Shanghai strain) by electroporation, the genomic DNA of all 21 transformants were prepared and hybridized with OmpL1. It showed that 6 of the 21 transfomants were recombinants in which the OmpL1 gene had been integrated into the genome of BCG.By immunoblotting with OmpL1 infected rabbit antiserum, which was preabsorbed to remove antibody against E. coli and SPAHRP, three recombinants, pLI1, pLI2 and pLI3, were detected to express OmpL1 protein. The ability of expression is in the order of pLI2>pLI1>>plI3. These studies provide the possibility of further research on the development of highly efficient recombinant vaccines against leptospirosis.

采用PCR技术直接从致病性钩端螺旋体(简称钩体)赖型017菌株基因组中扩增钩体外膜蛋白基因OmpL1,并克隆到大肠杆菌—卡介苗穿梭质粒载体pY6002中,重组质粒经电转化导入卡介苗。斑点杂交筛选重组卡介苗,再通过免疫印迹对其表达进行初步研究。在所得6个重组卡介苗中,有3个表达了OmpL1基因产物,其中一个表达较强。该研究为发展新一代高效广谱的钩体基因工程疫苗打下了基础。

In order to determine the characteristics of T cell immune responses induced by recombinant Mycobacterium bovis BCG(rBCG)expressing MalE protein of Escherichia coli (rBCG·MalE),the splenocytes from immunized mice were treated to deplete CD4 + or CD8 + T cells by an immunomagnetic selection,then these cells were used to measure the IFN_γ of IL_2 responses to MalE protein or its peptides in enzyme linked immunospot (ELISPOT)assay.The results clearly indicated that T cell immune responses induced by rBCG·MalE...

In order to determine the characteristics of T cell immune responses induced by recombinant Mycobacterium bovis BCG(rBCG)expressing MalE protein of Escherichia coli (rBCG·MalE),the splenocytes from immunized mice were treated to deplete CD4 + or CD8 + T cells by an immunomagnetic selection,then these cells were used to measure the IFN_γ of IL_2 responses to MalE protein or its peptides in enzyme linked immunospot (ELISPOT)assay.The results clearly indicated that T cell immune responses induced by rBCG·MalE were CD4 + T cell_dependent.The results of analyzing T cell responses to MalE protein or PPD in different time points ofter rBCG·MalE immunization showed that initial Thl responses to rBCG or BCG were modulated during the course of infection to become a mixed Th1/Th2 responses.These results revealed new mechanisms in T cell immune responese against mycobacteria,and further showed that BCG is an effective live vector to express and deliver foreign antigens to host immune system.

应用免疫磁性分离技术去除免疫小鼠脾脏细胞中CD4+或CD8+T细胞后 ,在酶联免疫斑点试验中证实表达大肠杆菌MalE蛋白的重组卡介苗 (rBCG·MalE)诱导的T细胞应答是CD4+T细胞依赖的。对MalE、PPD特异T细胞应答的动态分析结果表明 ,rBCG·MalE、BCG诱导的特异CD4+T细胞应答存在Th1/Th2平衡转换现象 ,即起始阶段为Th1应答 ,一段时间后出现Th2应答 ,并逐步形成Th1/Th2混合应答。这些结果 ,为分枝杆菌T细胞应答规律提供了新的认识 ,同时 ,亦表明BCG是优良的外源抗原表达与运送载体。

Genetic recombinant Bacille Calmette-Guerin(rBCG)was constructed by introducing the extraneous gene into BCG.The long-term cellular and humoral response was induced by rBCG.The new results showed that rBCG was an effective,low-lost vaccine against many sorts of infective dieases and tumours.

基因重组卡介苗 (rBCG)是借助分子生物学技术 ,将外源基因导入BCG中构建而成的多价疫苗 ,rBCG可诱导长期的体液免疫和细胞免疫 ,初步的研究结果已显示出rBCG具有广阔的应用前景 ,有望发展成为一种经济有效的新型疫苗以预防传染病和一些肿瘤

 
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