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   重组卡介苗 在 畜牧与动物医学 分类中 的翻译结果: 查询用时:0.362秒
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重组卡介苗
相关语句
  recombinant bcg
    INFECTION OF DENDRITIC CELLS BY RECOMBINANT BCG
    重组卡介苗感染树突细胞的研究
短句来源
    Under heat induced (45℃) , the expression recombinant protein was analyzed by SDS-PAGE and its relative molecular mass is 22kD. Our results may lay a foundation for constructing recombinant BCG vaccine against F. hepatica.
    45℃下热诱导FH3基因在重组卡介苗中的表达,并对基因表达产物进行SDS一PAGE分析,结果显示,重组卡介苗总蛋白在约22kD处有新的蛋白带出现,符合FH3基因序列表达产物大小。
短句来源
    The infection and antigen presenting cell functions of dendritic cells (DC) with recombinant BCG expressing MalE protein (rBCG·MalE ) from Escherichia coli were investigated.
    在体内外试验中 ,树突细胞 (DC)均能摄取表达大肠杆菌麦芽糖结合蛋白 (MalE)的重组卡介苗 (rBCG·MalE)。
短句来源
    Given current developments,it is anticipate that recombinant BCG and DNA vaccines are the most promising.
    目前看来,重组卡介苗和DNA疫苗被认为是最有前途的候选疫苗。
短句来源
  “重组卡介苗”译为未确定词的双语例句
    Kinetics of rBCG_initiated T Cell Immune Responses Monitored by ELISPOT Assay
    应用ELISPOT试验测定重组卡介苗诱导的T细胞应答
短句来源
    In order to determine the characteristics of T cell immune responses induced by recombinant Mycobacterium bovis BCG(rBCG)expressing MalE protein of Escherichia coli (rBCG·MalE),the splenocytes from immunized mice were treated to deplete CD4 + or CD8 + T cells by an immunomagnetic selection,then these cells were used to measure the IFN_γ of IL_2 responses to MalE protein or its peptides in enzyme linked immunospot (ELISPOT)assay.
    应用免疫磁性分离技术去除免疫小鼠脾脏细胞中CD4+或CD8+T细胞后 ,在酶联免疫斑点试验中证实表达大肠杆菌MalE蛋白的重组卡介苗 (rBCG·MalE)诱导的T细胞应答是CD4+T细胞依赖的。
短句来源
    Molecular and Cellular Mechanisms of T Cell Immune Responses Induced by Recombinant Mycobacterium bovis BCG
    重组卡介苗诱导T细胞免疫应答的分子、细胞机理研究
短句来源
    To identify T cell epitopes of expressed MalE protein from recombinant Mycobac-terium bovis BCG(rBCG·MalE) and their diversity. The epitope repertoire of expressed MalE was analyzed in vitro by antigen presentation assay using dendritic cells as APC pulsed with rBCG MalE,BCG·wt or purified MalE protein,then analyzed in vivo or ex vivo using T cell proliferation assay, IFN-γ-ELISPOT and epitope mapping from immunized mice.
    为鉴定重组卡介苗表达的MalE蛋白T细胞表位及其多样性,采用抗原提呈试验、T细胞增殖试验、ELISPOT试验和表位作图测试重组MalE蛋白的T细胞表位,结果重组BCG·MalE功能性表达了MalE蛋白的四种H-2d限制性T细胞表位。
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  recombinant bcg
Therefore, current studies aim at developing recombinant BCG (rBCG) strains secreting Th1-like cytokines to improve the effectiveness of the therapy.
      
Peripheral blood monocytes (PBMC) were stimulated by recombinant BCG and transformed into bacilli Calmette-Guerin activated killer (BAK) cells, and the effect of anticancer BAK cells was studied.
      
The result of MTT showed that the proliferation of PBMC in the recombinant BCG group was more powerful than in the other two groups (P >amp;lt; 0.05).
      
The result of LDH release assay showed that the antitumor activity of BAK cells stimulated by Recombinant BCG was the highest in all groups.
      
We conclude that the recombinant BCG can activate more PBMCs to anti-bladder cancer in vitro than wild-type BCG does.
      
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In order to determine the characteristics of T cell immune responses induced by recombinant Mycobacterium bovis BCG(rBCG)expressing MalE protein of Escherichia coli (rBCG·MalE),the splenocytes from immunized mice were treated to deplete CD4 + or CD8 + T cells by an immunomagnetic selection,then these cells were used to measure the IFN_γ of IL_2 responses to MalE protein or its peptides in enzyme linked immunospot (ELISPOT)assay.The results clearly indicated that T cell immune responses induced by rBCG·MalE...

In order to determine the characteristics of T cell immune responses induced by recombinant Mycobacterium bovis BCG(rBCG)expressing MalE protein of Escherichia coli (rBCG·MalE),the splenocytes from immunized mice were treated to deplete CD4 + or CD8 + T cells by an immunomagnetic selection,then these cells were used to measure the IFN_γ of IL_2 responses to MalE protein or its peptides in enzyme linked immunospot (ELISPOT)assay.The results clearly indicated that T cell immune responses induced by rBCG·MalE were CD4 + T cell_dependent.The results of analyzing T cell responses to MalE protein or PPD in different time points ofter rBCG·MalE immunization showed that initial Thl responses to rBCG or BCG were modulated during the course of infection to become a mixed Th1/Th2 responses.These results revealed new mechanisms in T cell immune responese against mycobacteria,and further showed that BCG is an effective live vector to express and deliver foreign antigens to host immune system.

应用免疫磁性分离技术去除免疫小鼠脾脏细胞中CD4+或CD8+T细胞后 ,在酶联免疫斑点试验中证实表达大肠杆菌MalE蛋白的重组卡介苗 (rBCG·MalE)诱导的T细胞应答是CD4+T细胞依赖的。对MalE、PPD特异T细胞应答的动态分析结果表明 ,rBCG·MalE、BCG诱导的特异CD4+T细胞应答存在Th1/Th2平衡转换现象 ,即起始阶段为Th1应答 ,一段时间后出现Th2应答 ,并逐步形成Th1/Th2混合应答。这些结果 ,为分枝杆菌T细胞应答规律提供了新的认识 ,同时 ,亦表明BCG是优良的外源抗原表达与运送载体。

The infection and antigen presenting cell functions of dendritic cells (DC) with recombinant BCG expressing MalE protein (rBCG·MalE ) from Escherichia coli were investigated. In both in vitro and in vivo assays, DC can capture rBCG·MalE. 1%~2% of the DC population was infected in vivo by the labeled rBCG·MalE through FACS analysis, a similar percentage of MΦ population was also found to be infected. It was shown that both DC and MΦ can phagocytose, process rBCG·MalE, and present T cell epitopes of MalE...

The infection and antigen presenting cell functions of dendritic cells (DC) with recombinant BCG expressing MalE protein (rBCG·MalE ) from Escherichia coli were investigated. In both in vitro and in vivo assays, DC can capture rBCG·MalE. 1%~2% of the DC population was infected in vivo by the labeled rBCG·MalE through FACS analysis, a similar percentage of MΦ population was also found to be infected. It was shown that both DC and MΦ can phagocytose, process rBCG·MalE, and present T cell epitopes of MalE protein to specific T hybridoma FBU·B11 using in vitro antigen presentation assay. Following infection of mice, DC and MΦ were purified and the presence of immunogenic peptide MHC class Ⅱ complexes could be detected ex vivo on sorted DC, but not on purified MΦ. These results suggest that DC play a pivotal role on the initiation of protective immune responses against mycobacteria.

在体内外试验中 ,树突细胞 (DC)均能摄取表达大肠杆菌麦芽糖结合蛋白 (MalE)的重组卡介苗 (rBCG·MalE)。应用流式细胞术测得荧光标记的rBCG·MalE在体内感染DC的水平在 1 % 2 % ,与感染巨噬细胞 (MΦ)的水平相当。体外抗原提呈试验结果显示 ,DC和MΦ能吞噬、加工rBCG·MalE并向T细胞杂交瘤FBU·B1 1提呈MalE蛋白抗原表位 ,而在体内试验中 ,从rBCG·MalE免疫小鼠脾脏中纯化的DC ,可检测到MalE表位MHCⅡ复合物 ,而纯化的MΦs却测不到。这表明DC在起始抗分枝杆菌的免疫保护中发挥关键作用。

In the past two decades,new molecular technologies were used to develop a novel more effective vaccine.New candidate tuberculosis vaccines,including attenuated live vaccine;recombinant live vaccine;subunit protein vaccine and DNA vaccine have increased markedly.Correspondingly,the advent vaccines have been tested in cattle.The candidate vaccines have their own advantages and disadvantages.Given current developments,it is anticipate that recombinant BCG and DNA vaccines are the most promising.However,the shared...

In the past two decades,new molecular technologies were used to develop a novel more effective vaccine.New candidate tuberculosis vaccines,including attenuated live vaccine;recombinant live vaccine;subunit protein vaccine and DNA vaccine have increased markedly.Correspondingly,the advent vaccines have been tested in cattle.The candidate vaccines have their own advantages and disadvantages.Given current developments,it is anticipate that recombinant BCG and DNA vaccines are the most promising.However,the shared and fatal disadvantage is their poor protective efficacy.So it is mandatory that a major effort be made to understand how animal body response to M.bovis and why vaccines have failed in some cases,so we can develop the next generation of bovine tuberculosis vaccines.

在过去的20年里,人们应用分子生物学技术来研制更为有效的结核病疫苗,新型候选疫苗大量涌现。这些新型疫苗主要包括减毒活疫苗、重组活疫苗、亚单位疫苗和核酸疫苗。对防制牛结核病的各种新型疫苗也进行了相应的研究,并取得了令人振奋的进展。各种新型疫苗各有优缺点。目前看来,重组卡介苗和DNA疫苗被认为是最有前途的候选疫苗。但是,所有候选疫苗共同的也是致命的缺点是免疫保护力低。因此,牛结核病疫苗研制的主要努力方向还是在研究分支杆菌免疫机制和免疫失败原因的基础上,进一步增强现有候选疫苗的免疫效力或研制更为有效的新型疫苗。

 
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