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重组卡介苗
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  recombinant bcg
    The pYUB295 recombinant BCG was identified by amplified recombinant BCG genome DNA and showed Ag85a gene was transformed into BCG successfully. The SDS-PAGE result of Ag85a recombinant BCG culture filtrate showed: the extrinsic Ag85a gene could be expressed in BCG.
    pYUB295-ag85a重组卡介苗基因组DNA的PCR扩增以及培养上清液的PAGE电泳表明:ag85a-卡介苗重组疫苗构建正确,Ag85a蛋白在卡介苗中分泌表达。
短句来源
    Studies and Application of Recombinant BCG Vaccines
    重组卡介苗的研究与应用
短句来源
    Construction, Expression and Identification of a Recombinant BCG Vaccine Encoding Human Mycobacterium tuberculosis Heat Shock Protein 65
    人结核杆菌热休克蛋白65重组卡介苗疫苗的构建、表达及鉴定
短句来源
    Construction of A Recombinant BCG Vaccine Expressing the Secretory Form Fusion Protein 85B-ESAT-6
    分泌性表达融合蛋白85B-ESAT-6重组卡介苗的构建和表达
短句来源
    Construction and Screen of Recombinant BCG Strain Expressing and Secreting Human Interleukin 12 Protein
    分泌表达人IL-12基因重组卡介苗菌株的构建和筛选
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  “重组卡介苗”译为未确定词的双语例句
    Identification of T cell epitopes of MalE protein expressed by recombinant Mycobacterium bovis BCG
    重组卡介苗表达的MalE蛋白T细胞表位的鉴定
短句来源
    The mean time to death and death rate in 2 month et al were used to valued the protection of BCG recombinant vaccines. Results: The gene of esat6 mpt64 ag85a ag85b were amplified by PCR. pYUB295 and PMD31 recombinants of these genes were constructed through recombinant technology and identified them by PCR enzyme digestion and DNA sequencing.
    方法:通过基因工程重组技术将结核分枝杆菌保护性抗原ESAT6、MPT64、Ag85A、Ag85B的编码基因分别与穿梭质粒载体PMD31、pYUB295重组,通过电穿孔技术导入卡介苗中,应用PCR扩增、PAGE电泳鉴定重组卡介苗
短句来源
    Objective To construct a recombinant fusion protein CFP10-ESAT6 of Mycobacterium tuberculosis expressed in BCG,the 633bp lhp-esat6 fusion gene was amplified by PCR from pQE30-CFP10-ESAT6 plasmid and then cloned into E.
    目的 构建能表达结核分枝杆菌早期分泌蛋白CFP10 -ESAT6融合蛋白的重组卡介苗 (recombinantBCG ,rBCG)。
短句来源
    Objective To construct phosphate-specific transport system 1(PstS1)-BCG recombinant vaccine.
    目的:构建结核分支杆菌分泌蛋白磷酸盐特异转运系统1(PstS1)的重组卡介苗
短句来源
    The resulting recombinant expression plasmid pBCG3000-85B-ESAT-6 was then transformed by electroporation into BCG, and induced by heating.
    用电穿孔法将pBCG3000-85B-ESAT-6质粒转化BCG细胞,得到重组卡介苗
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  recombinant bcg
Therefore, current studies aim at developing recombinant BCG (rBCG) strains secreting Th1-like cytokines to improve the effectiveness of the therapy.
      
Peripheral blood monocytes (PBMC) were stimulated by recombinant BCG and transformed into bacilli Calmette-Guerin activated killer (BAK) cells, and the effect of anticancer BAK cells was studied.
      
The result of MTT showed that the proliferation of PBMC in the recombinant BCG group was more powerful than in the other two groups (P >amp;lt; 0.05).
      
The result of LDH release assay showed that the antitumor activity of BAK cells stimulated by Recombinant BCG was the highest in all groups.
      
We conclude that the recombinant BCG can activate more PBMCs to anti-bladder cancer in vitro than wild-type BCG does.
      
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This study was intended to produce a new living vaccine against leptospirosis using BCG as vector. Leptospiral outer envelop antigen gene OmpL1 was amplified from the genome of pathogenic leptopira serova Lai 017 by PCR, and cloned in E.coliBCG shuttle plasmid pY6002. Recombinant plasmids were isolated by dot blotting with Digoxigeninlabeled OmpL1 gene. After transforming the recombinant plasmids in BCG (Shanghai strain) by electroporation, the genomic DNA of all 21 transformants were prepared and hybridized...

This study was intended to produce a new living vaccine against leptospirosis using BCG as vector. Leptospiral outer envelop antigen gene OmpL1 was amplified from the genome of pathogenic leptopira serova Lai 017 by PCR, and cloned in E.coliBCG shuttle plasmid pY6002. Recombinant plasmids were isolated by dot blotting with Digoxigeninlabeled OmpL1 gene. After transforming the recombinant plasmids in BCG (Shanghai strain) by electroporation, the genomic DNA of all 21 transformants were prepared and hybridized with OmpL1. It showed that 6 of the 21 transfomants were recombinants in which the OmpL1 gene had been integrated into the genome of BCG.By immunoblotting with OmpL1 infected rabbit antiserum, which was preabsorbed to remove antibody against E. coli and SPAHRP, three recombinants, pLI1, pLI2 and pLI3, were detected to express OmpL1 protein. The ability of expression is in the order of pLI2>pLI1>>plI3. These studies provide the possibility of further research on the development of highly efficient recombinant vaccines against leptospirosis.

采用PCR技术直接从致病性钩端螺旋体(简称钩体)赖型017菌株基因组中扩增钩体外膜蛋白基因OmpL1,并克隆到大肠杆菌—卡介苗穿梭质粒载体pY6002中,重组质粒经电转化导入卡介苗。斑点杂交筛选重组卡介苗,再通过免疫印迹对其表达进行初步研究。在所得6个重组卡介苗中,有3个表达了OmpL1基因产物,其中一个表达较强。该研究为发展新一代高效广谱的钩体基因工程疫苗打下了基础。

Objective To construct a recombinant BCG secretively expressing ESAT 6 of Mycobacterium tuberculosis. Methods α antigen(α Ag) signal sequence and esat 6 gene were amplified from the genome of Bacille Calmette Guerin (BCG) and Mycobacterium tuberculosis by PCR respectively. esat 6 gene was cloned in E.coli BCG shuttle plasmid pMV261 to get pME . Then a new recombinant plasmid pSME was constructed by inserting BCGα Ag signal sequence into pME. Results The cloned genes α Ag signal sequence...

Objective To construct a recombinant BCG secretively expressing ESAT 6 of Mycobacterium tuberculosis. Methods α antigen(α Ag) signal sequence and esat 6 gene were amplified from the genome of Bacille Calmette Guerin (BCG) and Mycobacterium tuberculosis by PCR respectively. esat 6 gene was cloned in E.coli BCG shuttle plasmid pMV261 to get pME . Then a new recombinant plasmid pSME was constructed by inserting BCGα Ag signal sequence into pME. Results The cloned genes α Ag signal sequence and esat 6 were correctly inserted into the vector pMV261,which was confirmed by restriction endonuclease digestion and PCR amplification of pSME. Conclusion pSME was expected to secretively express ESAT 6 of Mycobacterium tuberculosis in BCG.This study provides the possibility of further researches on the development of new anti tuberculosis vaccine.

目的 构建分泌性表达结核分枝杆菌抗原蛋白 ESAT- 6的重组卡介苗。方法 分别以卡介苗(BCG)和结核分枝杆菌 H37Rv株基因组 DNA为模板 ,通过 PCR扩增得到约 117bp的 BCGα抗原 (α- Ag)信号肽序列和 2 85 bp的结核杆菌 esat- 6基因序列。将 esat- 6基因与大肠杆菌 -卡介苗穿梭质粒载体 p MV2 6 1重组 ,得到重组质粒 p ME。再将 BCGα- Ag信号肽序列克隆至 p ME中 ,得到重组质粒 p SME。结果 质粒 p SME用双酶切和 PCR扩增鉴定证实 ,克隆基因 α- Ag信号肽序列和 esat- 6正确插入载体 p MV2 6 1。结论 重组质粒 p SME可望在 BCG中分泌性表达结核分枝杆菌的免疫保护性抗原蛋白 ESAT- 6 ,该质粒的构建成功为改造卡介苗、发展新型结核病疫苗奠定了基础

Objective To identify T cell MalE epitopes protein by recombinant Mycobacterium bovis BCG(rBCG MalE). Methods The epitope repertoire of expressed MalE was analyzed in vitro by antigen presentation assay using dendritic cells as APC pulsed with rBCG.MalE, BCG wt or purified MalE protein, and further analyzed in vivo or in vitro using T cell proliferation assay, IFN-γ-ELISPOT and epitope mapping from immunized mice. Results rBCG MalE functionally expressed all 4 H-2 d restricted T cell epitopes of native...

Objective To identify T cell MalE epitopes protein by recombinant Mycobacterium bovis BCG(rBCG MalE). Methods The epitope repertoire of expressed MalE was analyzed in vitro by antigen presentation assay using dendritic cells as APC pulsed with rBCG.MalE, BCG wt or purified MalE protein, and further analyzed in vivo or in vitro using T cell proliferation assay, IFN-γ-ELISPOT and epitope mapping from immunized mice. Results rBCG MalE functionally expressed all 4 H-2 d restricted T cell epitopes of native MalE. Conclusion The p68-82 of expressed MalE was immunodominant epitope and the others were subdominant epitopes.

目的 鉴定重组卡介苗表达的MalE蛋白T细胞表位。方法 用抗原提呈试验、T细胞增殖试验、ELISPOT试验和表位作图测试重组MalE蛋白的T细胞表位。结果 重组BCG MalE功能性表达了MalE蛋白的 4种H 2 d 限制性T细胞表位。结论 在 4种表位中 ,p6 8~ 82是重组MalE蛋白的主要T细胞表位 ,而其余 3个为次要表位

 
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