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磷酸化反应
相关语句
  phosphating reaction
     Phosphating Reaction and Its Application in Leather Industry
     磷酸化反应及其在皮革中的应用
短句来源
     Synthetic principles of ester exchanging reaction and phosphating reaction were investigated.
     探讨了酯交换反应和磷酸化反应的合成原理。
短句来源
  phosphorylation reaction
     The effect of Ca 2+ and Mn 2+ on secondary metabolism of Amycolatopsis mediterranei U 32 were studied using co culture method of filter paper slice and phosphorylation reaction in vitro to detecting antibiotics content and protein kinase activities.
     采用Ca2 + 、Mn2 + 和地中海拟无枝菌酸菌U 32共培养的方法 ,通过滤纸法检测抗生素含量和离体磷酸化反应检测激酶活性 ,研究Ca2 + 和Mn2 + 对地中海拟无枝菌酸菌U 32次生代谢的影响。
短句来源
     The subunit molecular weight is about 55×10 3. The enzyme activity assaying system is excessive ATP in standard reaction system. The surplus ATP of phosphorylation reaction was extracted then was decomposed by chloroplast Mg 2+ ATPase. After that the inorganic phosphorus was assayed by combined malachite green reagent.
     纯化的 Ca2 + / Ca M依赖性蛋白激酶经 SDS-聚丙烯酰胺凝胶线性梯度电泳 ,亚基分子量约为 5 5× 10 3.在 Ca2 + / Ca M依赖性蛋白激酶的标准反应体系中 ,存在过量的 ATP,提取磷酸化反应剩余的 ATP,用叶绿体 Mg2 + - ATP酶分解 ATP,最后用复合孔雀绿测定无机磷 ,从而来计算 Ca2 + / Ca M依赖性蛋白激酶的活性 .
短句来源
     The organosilicon grafted castor oil could undertake phosphorylation reaction easily at lower temperature. The average molecular weight of phosphorylated castor oil was ca. 1 200 and it had favorable stability.
     在较低的温度下,蓖麻油接枝产物可以发生磷酸化反应,生成的磷酸化蓖麻油平均相对分子质量为1 200左右,乳液稳定。
短句来源
  “磷酸化反应”译为未确定词的双语例句
     There was no measurable changes in tyrosine phosphorylation of STAT1,STAT3, STAT5a or STAT5b, nor activation of MAP kinases including ERK 1 /2, p38, and JUNK 1/2 in liver by 3 days of fasting.
     细胞内信号传递蛋白STAT1、STAT3、STAT5a和STAT5b均无酪氨酸磷酸化反应,MAP激酶中的ERK1、ERK2、P3S、JUNK1,JUNK2均无激活表现。
短句来源
     The optimum condition is as follows: n(-OH)∶n(P_2O_5) is 2.0∶1.0,esterification time is 3.5 hours,esterification temperature is 80 ℃,the dosage of H_2O is 4% PP, hydrolisis temperature is 50 ℃,hydrolisis time is 1.5 hours.
     采用正交试验研究了影响聚醚改性硅氧烷磷酸化的主要因素,得出磷酸化反应最优条件为:反应物中n(-OH)∶n(P2O5)为2.0∶1.0,酯化时间为3.5h,酯化温度为80℃,水解量为聚醚改性硅氧烷磷酸酯(简称PP)的4%,水解温度为50℃,水解时间为1.5h。
短句来源
     The optimum conditions of the phosphonation are as follow:-OH/P_2O_5/H_2O(molar ratio)=2.4∶1∶1.15,temperature of reaction is 70 ℃ for 240 min. The hydrolysis reaction takes at 60 ℃ for 60 min.
     磷酸化反应的工艺条件是n(-OH)∶n(P2O5)∶n(H2O)=2 4∶1∶1 15,70℃下反应4h,60℃下水解1h。
短句来源
     The optimum phosphorylation medium was an aqueous solution maintaining pH 12 0~12 5 at 0~10℃ and containing a high concentration of pyridine (the initial concentration above 2.2mol/L).
     最佳磷酸化反应介质为一保持pH120~125并含高浓度吡啶(初始浓度大于22mol/L)的0~10℃的水溶液。
短句来源
     Hypoxic PC activated PKC in VSMC and potentiated phosphorylation mediated by PKC.
     缺氧PC激活VSMC内PKC,并使PKC介导的蛋白磷酸化反应加强。
短句来源
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  相似匹配句对
     Protein phosphorylation in disease resistance response of plant
     植物抗病反应中的蛋白磷酸化
短句来源
     Phosphating Reaction and Its Application in Leather Industry
     磷酸化反应及其在皮革中的应用
短句来源
     From the result of above tests, optimal reaction solution was obtained.
     反应条件。
短句来源
     Its high activity and selectivity for Heck reactions were measured;
     BuHeek反应
短句来源
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  phosphorylation reaction
This number was reduced to about five under EGTA plus cyclic AMP conditions, whereas the presence of EGTA alone suppressed the phosphorylation reaction almost totally.
      
Soman did not show this effect, evidently because of the high velocity of the phosphorylation reaction.
      
Optimum experimental conditions have been explored, and the effects of Mn2+ and phosphocreatine (PCr) on the determination of the phosphorylation reaction have been assayed.
      
The method was used to monitor the time courses of the phosphorylation reaction in solution.
      
An attempt is made to compare the phosphorylation reaction with visual adaptation hypotheses taken from the electrophysiological literature.
      
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In a previous paper we reported that under flashes of the same duration and intensity, the nonoyclic PSP [FMN, Vit. K or Fe (CN)_6~≡ system] requires a longer dark time than the cyclic PSP (PMS system) to produce maximum flash yields (MFY) indicating that a longer dark reaction pathway exists in the former system. There were indications that they may differ also in the photochemical reaction paths, since their behavior toward o-phenanthroline (a photochemical inhibitor) is different. It was also reported that...

In a previous paper we reported that under flashes of the same duration and intensity, the nonoyclic PSP [FMN, Vit. K or Fe (CN)_6~≡ system] requires a longer dark time than the cyclic PSP (PMS system) to produce maximum flash yields (MFY) indicating that a longer dark reaction pathway exists in the former system. There were indications that they may differ also in the photochemical reaction paths, since their behavior toward o-phenanthroline (a photochemical inhibitor) is different. It was also reported that the noncyclic PSP has a higher MFY than the cyclic and both are affected by temperature. But the light flashes formerly used were rather long and interpretation was difficult. It is desirable to compare the two systems under shorter flashes of high intensity.These conditions were obtained by using natural sunlight reflected by a rotating mirror. The rapidly passing beam was focussed by a convex lens to the reaction vessel (Fig. 1). Flashes of 3.3×10~(-4) sec. with dark interval of 0.16 sec. could be obtained and the intensity was found to be saturating (Table 1). Long light flashes were obtained with rotating disc as in the previous paper. Preparation of reaction mixtures and analytic procedures were as earlier described.The following results were obtained:(1) Under short flashes (3.3 x 10~(-4) sec.)similar MFY are found in both cyclic and noncyclic PSP systems [PMS, Vit. K, FMN, Fe (CN)_6~≡]. And the MFY is not significantly affected by temperature (Table 2).(2) The concentration of o-phenanthroline for 50% inhibition of the MFY is 4.5×10~(-5) M in the PMS system and 0.95×10~(-5) M in the Vit. K (Fig. 2), FMN and Fe(CN)_6~≡ systems (Fig. 3).(3) In continuous light of low intensity (250lux) the concentration of o-phenanthroline for 50% inhibition of PSP is 0.95×10~(-5)M for the Vit. Kand 4.5×10~(-5) M for the PMS systems, being the same as in short flashes of saturating intensity. But in continuous light of high intensities (100,000lux) the concentration of the inhibitor required is higher in both cases. It is still higher if the temperature is increased from 5 to 30℃ (Fig. 2 and 4, Table 3). The temperature quotient (Q_(10) for the PMS system is 1.2 and for the Vit. K system 2.6.(4) The inhibition of MFY by o-phenanthroline is not affected by flash intensity (Vit. K system, Fig. 5) but is decreased by increasing flash duration (Fig.6 and 7).Two conclusions are tentatively drawn from the above results. (A) o-phenanthroline inhibits primarily the photochemical reaction center. The decreases in inhibition affected by longer flash durations and in continuous light by higher light intensities and higher temperatures are probably due to the increased rate of dark reactions leading to a more rapid turnover, i. e. repetition of the photochemical reaction center. (B) The same MFY in both cyclic (PMS) and noncyclic (Vit. K etc.) PSP shows that the number of phosphorylation site is the same in both systems. The higher sensitivity toward o-phenanthroline in the later system indicates that it has probably two photochemical reaction centers, one of which is concerned with O_2 evolution which is lacking in the former system.

在暗間隔0.16秒的3.3×10~(-4)秒閃光下,比較了PMS,Vit.K,FMN及Fe(CN)_6~≡光合磷酸化反应系統的每閃最高产量以及温度的影响,并观察邻二氮杂菲对这些反应系統每閃最高产量抑制的差异,以及在閃时延长时,它对閃光产量抑制程度的变化,得到結果如下: (1)在短閃光下,PMS,Vit.K,FMN及Fe(CN)_6~≡光合磷酸化反应系統的每閃最高产量都极接近,温度的影响也不太显著。(2)邻二氮杂菲对Vit.K,FMN及Fe(CN)_6~≡非循环光合磷酸化反应系統的每閃最高产量的50%抑制濃度都在0.96×10~(-5)M/左近,而对PMS系統的每閃最高产量的50%抑制濃度則力4.5×10~(-5)M。(3)邻二氮杂菲对PMS及Vit.K这两种类型的光合磷酸化反应系統在連續弱光下反应速度的50%抑制濃度与对这两反应系統的每閃最高产量的50%抑制濃度相同,即分别为4.5×10~(-5)M及0.95×10~(-5)M;同样温度(5℃)而光强增加到100000米烛光,则对这两反应系统反应速度的50%抑制浓度提高到依次为14.0×10~(-5)M及2.3×...

在暗間隔0.16秒的3.3×10~(-4)秒閃光下,比較了PMS,Vit.K,FMN及Fe(CN)_6~≡光合磷酸化反应系統的每閃最高产量以及温度的影响,并观察邻二氮杂菲对这些反应系統每閃最高产量抑制的差异,以及在閃时延长时,它对閃光产量抑制程度的变化,得到結果如下: (1)在短閃光下,PMS,Vit.K,FMN及Fe(CN)_6~≡光合磷酸化反应系統的每閃最高产量都极接近,温度的影响也不太显著。(2)邻二氮杂菲对Vit.K,FMN及Fe(CN)_6~≡非循环光合磷酸化反应系統的每閃最高产量的50%抑制濃度都在0.96×10~(-5)M/左近,而对PMS系統的每閃最高产量的50%抑制濃度則力4.5×10~(-5)M。(3)邻二氮杂菲对PMS及Vit.K这两种类型的光合磷酸化反应系統在連續弱光下反应速度的50%抑制濃度与对这两反应系統的每閃最高产量的50%抑制濃度相同,即分别为4.5×10~(-5)M及0.95×10~(-5)M;同样温度(5℃)而光强增加到100000米烛光,则对这两反应系统反应速度的50%抑制浓度提高到依次为14.0×10~(-5)M及2.3×10~(-5)M。如果再把温度从5℃提升到30℃,则50%抑制浓度更提高到25.0×10~(-5)M及4.0×10~(-5)M。(4) 邻二氮杂菲对Vit.K系统的短闪光的每闪最高产量的抑制程度不受闪光光强影响,然而当闪光的闪时增加时,抑制剂对闪光产量的抑制程度则随闪时增加而减少。由此推论,邻二氮杂菲的抑制部位与光合磷酸化反应的光化作用中心有关,增加闪光闪时,或在连续光下增加光强,或在饱和光下提高温度都使光化作用中心增加重复利用而使抑制剂的抑制作用减弱。循环(PMS)及非循环(Vit.K,FMN,Fe(CN)_6~≡)光合磷酸化反应系统的闪光产量相同,而对邻二氮杂菲的敏感程度却相差很大的现象,可以用放氧及磷酸化各有一光化作用中心解释之。非循环光合磷酸化反应系统包括有放氧及磷酸化两个,而循环光合磷酸化反应系统则仅需磷酸化一个光化作用中心。

Cardiac hemodynamics and myocardial utilization of carbohydrates during early stage of acute potassium antimony tartrate (PAT) intoxication were investigated in order to elucidate the relationship between functional and metabolic disturbances of heart. In 12 dogs, cardiac output was estimated by Fick's principle while coronary blood flow and myocardial metabolism were studied by coronary sinus catheterization. This study was designed in paired experiments. PAT 10 mg/kg was administered intravenously. Both control...

Cardiac hemodynamics and myocardial utilization of carbohydrates during early stage of acute potassium antimony tartrate (PAT) intoxication were investigated in order to elucidate the relationship between functional and metabolic disturbances of heart. In 12 dogs, cardiac output was estimated by Fick's principle while coronary blood flow and myocardial metabolism were studied by coronary sinus catheterization. This study was designed in paired experiments. PAT 10 mg/kg was administered intravenously. Both control and experimental observations were carried out on the same dog, at intervals of 1—2 weeks. The results of both periods were analyzed by t test of individual and group comparisons, aimed to exclude the possible influences other than PAT. During the first hour of the control period, the hemodynamic changes were insignificant, while both arterial and venous levels of lactic acid and pyruvic acid were elevated and the myocardial consumption of oxygen and pyruvic acid was augmented. During the 4th hour of the control period, the aortic blood pressure, total peripheral resistance and pulmonary vascular resistance were increased, while the cardiac index and the work of both ventricles reduced. These alterations might be ascribed essentially to the action of the anesthetic-sodium pentobarbital. One hour after PAT administration, the work of both ventricles was apparently not altered, while arterial and venous levels of glucose, lactic acid and pyruvic acid were elevated, A—V difference and myocardial consumption of lactic acid were increased. The oxygen extraction ratios of carbohydrates were likewise augmented. The mechanical efficiency of left ventricle was diminished as a result of augmented energy cost without corresponding increase of work. Four hours after PAT administration, aortic and pulmonary blood pressure, work of both ventricles and coronary blood flow were all decreased. Meanwhile, the myocardial energy cost was not reduced correspondingly. Hence, the mechanical efficiency of left ventricle was lowered. Thus it was deduced that cardiac functional impairment during PAT intoxication was due to metabolic derangement of the heart. However, the myocardial extraction of carbohydrates remained a linear function of their heightened arterial concentration, and the percentage of myocardial anaerobic metabolic rate was not increased. So it was suggested that the aerobic myocardial metabolism was not impaired, and that the lowered mechanical efficiency resulted probably from the impairment of storage or utilization of energy. The elevated level of glucose, lactic acid and pyruvic acid in blood during early stage of PAT intoxication indicated that the anaerobic glycolysis of the body as a whole was enhanced.

为闡明吐酒石心肌中毒时代謝与机能障碍的关系,研究了急性中毒早期狗心血流动力和心肌利用糖类能源的变化。狗12只,按Fick原理測心輸出量,用冠状窦插管法研究冠脉流量和心肌代謝。采用配对实驗,每狗实驗2次,观察对照期和肺动脉內注射吐酒石10毫克/公斤后变化。实驗結果作t测驗个别比較和组間比較以排除吐酒石外的影响因素。对照期1°时心脏血流动力变化不显著,但动靜脉中乳酸、丙酮酸增高,心肌耗氧量、丙酮酸消耗量增加。4°时主动脉压、总外周阻力和肺血管阻力增高,心脏指数与左右心室作功量减少。这些可能主要是戊巴比妥鈉的作用。吐酒石后1°,左右心室作功量不增加,而动靜脉中糖、乳酸、丙酮酸增加,乳酸的动靜脉差和心肌消耗量增加,糖类在心肌內氧化时所消耗氧量占心肌耗氧总量的%增加。因作功不增而耗能增加,故左室功率降低。吐酒石后4°主动脉压、肺动脉压、左右心室作功量及冠脉流量均减少,而心肌耗能量不减少,故左室功率也减少。結果說明吐酒石損害心肌机能与心肌代謝紊乱有关。但心肌仍能按比例提取糖类能源,且心肌无氧代謝%不增,說明心肌有氧代謝(能量釋放)无障碍。推測心功率降低可能是干扰了氧化磷酸化反应(能量貯存)或能量轉换(能量利用...

为闡明吐酒石心肌中毒时代謝与机能障碍的关系,研究了急性中毒早期狗心血流动力和心肌利用糖类能源的变化。狗12只,按Fick原理測心輸出量,用冠状窦插管法研究冠脉流量和心肌代謝。采用配对实驗,每狗实驗2次,观察对照期和肺动脉內注射吐酒石10毫克/公斤后变化。实驗結果作t测驗个别比較和组間比較以排除吐酒石外的影响因素。对照期1°时心脏血流动力变化不显著,但动靜脉中乳酸、丙酮酸增高,心肌耗氧量、丙酮酸消耗量增加。4°时主动脉压、总外周阻力和肺血管阻力增高,心脏指数与左右心室作功量减少。这些可能主要是戊巴比妥鈉的作用。吐酒石后1°,左右心室作功量不增加,而动靜脉中糖、乳酸、丙酮酸增加,乳酸的动靜脉差和心肌消耗量增加,糖类在心肌內氧化时所消耗氧量占心肌耗氧总量的%增加。因作功不增而耗能增加,故左室功率降低。吐酒石后4°主动脉压、肺动脉压、左右心室作功量及冠脉流量均减少,而心肌耗能量不减少,故左室功率也减少。結果說明吐酒石損害心肌机能与心肌代謝紊乱有关。但心肌仍能按比例提取糖类能源,且心肌无氧代謝%不增,說明心肌有氧代謝(能量釋放)无障碍。推測心功率降低可能是干扰了氧化磷酸化反应(能量貯存)或能量轉换(能量利用)的結果。吐酒石急性中毒早期,血中糖、乳酸、丙酮酸相应升高,提示全身无氧糖酵解加强。

The present paper deals with the respiration and oxidative phosphorylation in the isolated flight muscle mitochondria of the armyworm moth. The experimental results obtained showed that mitochondrial preparations from this insect not only oxidized many citric acid cycle intermediates (pyruvate plus malate, citrate, α-ketoglutarate, succinate, fumarate and malate), but also rapidly oxidized α-glycerophosphate and glutamate. In a phosphorylating medium the respiratory rate (Q_(O_2)) varied considerably with the...

The present paper deals with the respiration and oxidative phosphorylation in the isolated flight muscle mitochondria of the armyworm moth. The experimental results obtained showed that mitochondrial preparations from this insect not only oxidized many citric acid cycle intermediates (pyruvate plus malate, citrate, α-ketoglutarate, succinate, fumarate and malate), but also rapidly oxidized α-glycerophosphate and glutamate. In a phosphorylating medium the respiratory rate (Q_(O_2)) varied considerably with the substrates used. The Q_(O_2), of α-glycerophosphate was several times (2.5—6.0) higher than those of some citric acid cycle intermediates and glutamate. However, it was only 1.2 times that of succinate. Pyruvate (plus malate) was oxidized at the lowest rate (Q_(O_2)=16.6), which could be increased by the addition of some cofactors, such as CoA, thiamine pyrophosphate, NAD~+ and NADP~+, and under such conditions the Q_(O_2), of pyruvate as high as 50 was obtained. Combinations of pyruvate with some of the citric acid cycle intermediates individually also significantly enchanted the respiratory rate of mitochondrial preparations, which may approach or reach the level of α-glycerophosphate oxidation.It was demonstrated that a significant portion of the esterifieation of inorganic phosphate was coupled with the oxidation of those substrates. The average P/O ratios nearly reached the corresponding theoretical values. In contrast to the mitochondrial preparations from migratory locust and some other insects, the isolated armyworm moth mitochondria did not need bovine serum or plasma albumin for the coupling of phosphorylation with oxidation.2,4-Dinitrophenol was capable of uncoupling the reactions of phosphorylation from oxidation, as well as stimulating the latent ATPase activity in the mitochondria. In addition, the freshly prepared mitochondria also exhibited a strong Mg~(++)-sti- mulated ATPase activity, which was found to be inhibited partly by the additon of Ca~(++).Changes in the respiration and effeciency of oxidative phosphorylation during adult development (1, 2, 3, 4 and 8 days after emergence of the moth) have also been investigated and the results indicated that Q_(O_2), and P/O ratios of the mitochondria varied slightly with age. Generally, the mitochondria from the first-day moth were characterized by the low Q_(O_2)'S of pyruvate, α-glycerophosphate and succinate, and also by the low P/O ratio of glutamate.Finally, the problems concerning the characteristics of energy metabolism in the flight muscles of the armyworm moth and the relative importance of α-glycerophosphate and pyruvate oxidation via citric acid cycle in the maintenance of energy expenditure in muscle contraction during flight were discussed.

离体的粘虫蛾胸肌綫粒体制剂不仅能氧化三羧酸循环各中間产物,如丙酮酸(加苹果酸),檸檬酸,α-酮戊二酸,琥珀酸,延胡索酸以及苹果酸,而且尚能迅速氧化α-甘油磷酸和谷氨酸。在有磷酸受体系統存在时,α-甘油磷酸的呼吸率最高(Q_(O_2)值平均为101.4),比上述其他各底物高2.5—6.0倍,但仅比琥珀酸高1.2倍。丙酮酸的氧化速率最低(Q_(O_2),值平均为16.6),在后种情况下,反应系統中再加入輔酶A,輔羧酶,NAD~+以及NADP等輔助因子,可使丙酮酸的Q_(O_2)值提高2—3倍而达到50左右。丙酮酸与三羧循环各中間产物等分别組合进行同时氧化时,可使綫粒体的呼吸率接近或达到α-甘油磷酸的Q_(O_2)水平。在上述各底物氧化时,均表現偶联的呼吸鏈磷酸化反应。各底物的P/O比值基本上接近或达到相应的理論值。与东亚飞蝗和其他昆虫綫粒体制剂不同,粘虫蛾胸肌綫粒体的偶联磷酸化反应并不需要血浆清蛋白的保护。2,4-二硝基酚可使氧化和磷酸化发生解偶联現象,并激活綫粒体制剂的“潜在”ATP酶活力。同时,新鮮的粘虫胸肌綫粒体亦表現較高的Mg~(++)激活的ATP酶活力,后者,并受到Ca~(++)...

离体的粘虫蛾胸肌綫粒体制剂不仅能氧化三羧酸循环各中間产物,如丙酮酸(加苹果酸),檸檬酸,α-酮戊二酸,琥珀酸,延胡索酸以及苹果酸,而且尚能迅速氧化α-甘油磷酸和谷氨酸。在有磷酸受体系統存在时,α-甘油磷酸的呼吸率最高(Q_(O_2)值平均为101.4),比上述其他各底物高2.5—6.0倍,但仅比琥珀酸高1.2倍。丙酮酸的氧化速率最低(Q_(O_2),值平均为16.6),在后种情况下,反应系統中再加入輔酶A,輔羧酶,NAD~+以及NADP等輔助因子,可使丙酮酸的Q_(O_2)值提高2—3倍而达到50左右。丙酮酸与三羧循环各中間产物等分别組合进行同时氧化时,可使綫粒体的呼吸率接近或达到α-甘油磷酸的Q_(O_2)水平。在上述各底物氧化时,均表現偶联的呼吸鏈磷酸化反应。各底物的P/O比值基本上接近或达到相应的理論值。与东亚飞蝗和其他昆虫綫粒体制剂不同,粘虫蛾胸肌綫粒体的偶联磷酸化反应并不需要血浆清蛋白的保护。2,4-二硝基酚可使氧化和磷酸化发生解偶联現象,并激活綫粒体制剂的“潜在”ATP酶活力。同时,新鮮的粘虫胸肌綫粒体亦表現較高的Mg~(++)激活的ATP酶活力,后者,并受到Ca~(++)的部分抑制。不同发育日龄的粘虫蛾胸肌綫粒体的呼吸率和P/O比值也略呈差异,并与氧化底物有关。羽化后第一天,丙酮酸的Q_(O_2)值較低,第四天以后即增高并趋恒定。P/O比值除谷氨酸在第一天略低外,一般均不因发育日龄而显著变化。本文討論了粘虫蛾飞翔肌綫粒体能量代謝的若干特点井此較了α-甘油磷酸和丙酮酸-三羧酸循环底物的氧化在維持昆虫飞翔肌的能量需要方面的重要性。同时对肌綫粒体Mg~(++)-激活的ATP酶的功能和来源的問題也略加討論。

 
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