Methods In a field trial on human metabolism, 3.0mg per subject of iron with 57 Fe enriched was orally administrated, and feces were collected by fecal monitoring. The isotope ratio of 57 Fe to 56 Fe in the prepared fecal sample was detected with the ORS-ICP-MS.
Conclusion The ratio of 57 Fe to 56 Fe in the fecal sample could be accurately determined by the quadrupole ICP-MS with ORS, based on which the dietary iron absorption could be evaluated and the metabolism of iron in the human body could also be traced.
PCR and denaturing gradient gel electrophoresis(DGGE) were used to track the development of fecal bacterial community of 5 diarrhea piglets. Clone libraries were created from complete 16S rDNA of 42-day fecal sample of piglet 1,2 and 5. From the library,clones had their V6-V8 regions matched predominant bands on the DGGE gel were then sequenced and their 16S rDNAs subjected to an online similarity search.
The study found that the average bacterial numbers in all feces from six Giant Pandas in three age groups were 10 3cfu/g～10 7cfu/g,in which,the bacterial numbers of feces in young,adult and old Giant Pandas were 7 3×10 5cfu/g,7 8×10 5cfu/g and 2 0×10 6cfu/g,respectively.
The fecal sample was extracted with acetonitrile, defatted with hexane, cleaned-up using C18 cartridge.
Efficacy was assessed by analyzing the reduction in fecal egg counts of a fecal sample taken between days 7 to 1 before treatment in comparison to a fecal sample taken between days 7 to 13 after treatment.
Genomic DNA was extracted directly from fecal sample of each animal and nested PCR was performed to amplify part of the 18S ribosomal RNA (18SrRNA) of the Cryptosporidium species.
Somatic coliphage concentrations in feces of feral pigeons were typically low with an occasional fecal sample having high numbers of either one of the two types of phages seen in this population of birds.
By contrast, the number of fruit species per gorilla fecal sample (average 0.8 species) was much lower than that for gorillas in the lowland habitats.
The object of this research is to make sure of the application of acid insoluble ash(AIA)as indicator to the dairy goat dagestion trials. Six Xi Nong Saanen dairy goats were selected and tests were made in specially constructed metabolism cages. The means and standard deviations of the recoveries of natural indicators SiO_and AIA(concentrated HCl,4N HCl and 2N HCl)in the goatf ecals are 97.9±10.5%,96.7±1.96,100.7±1.23% and 105.7±1.98% respectively.It is therefore concluded that only the mean recovery of 4N HCl-AIA...
The object of this research is to make sure of the application of acid insoluble ash(AIA)as indicator to the dairy goat dagestion trials. Six Xi Nong Saanen dairy goats were selected and tests were made in specially constructed metabolism cages. The means and standard deviations of the recoveries of natural indicators SiO_and AIA(concentrated HCl,4N HCl and 2N HCl)in the goatf ecals are 97.9±10.5%,96.7±1.96,100.7±1.23% and 105.7±1.98% respectively.It is therefore concluded that only the mean recovery of 4N HCl-AIA is not significantly different(P>0.05)from 100%.The digestibility of dry matter, crude ash,gross energy,ether extract,crude fiber,crude protein and nitrogen- free-extract in ration determined by SiO_2 and AIA indicator mentioned above showed that only all the values determined by the method of 4N HCl-AIA near (P>0.05)the traditional method of collecting totol fecal.After quantitative feeding,the fecal samples were collected and the 2N HCl-AIA content dairy was analysed for two weeks.From ihe 6th day on,constant(P>0.05)value determined daily appeared.The 2N HCl-AIA contents of the fecal samples in different fecal collec-tion period were also determined,and the mixed samples of the 6th,7th,and 8th days showed no significant difference(P>0.05). On the 15th day after quantitativa feeding the fecal samples were collected at interval of 4-hour a day(6 times ewery day)and analysed.The 4N HCl-AIA contents of the fecal samples collected at any 4-hour interval showed no signiticant difference(P>0.05)from the whole fecal samplescollected in the same day.
Four human rotavirus strains were isolated from 7 fecal specimens of pediatric patients with acute diarrhea and underwent 14 passages in CV-1 cell culture.The replication and specificity of these isolates in cell culture were confirmed by characteristic cytopathic effects, EM, ELISA, IF and viral RNA electrophoresis. All of the 4 isolates were long electrophero-type and no difference in RNA patterns was observed between the original fecal extract and the strain adapted in CV-1 cell culture for 7-14 passages.
In view of the exceptionally important role of the medical radiation protection in human health? in this paper we try to study on the monitoring of internal contamination of radionuclides in organism by different autoradiogra-phic methods* such as: monitoring of the body retention of isolated or combined radionuclides by freezing microautoradiography; monitoring of blood>bone marrow and excreta radioactive samples by smear autoradiographyi differentiation of two radionuclides contamination by double radionuclide...
In view of the exceptionally important role of the medical radiation protection in human health? in this paper we try to study on the monitoring of internal contamination of radionuclides in organism by different autoradiogra-phic methods* such as: monitoring of the body retention of isolated or combined radionuclides by freezing microautoradiography; monitoring of blood>bone marrow and excreta radioactive samples by smear autoradiographyi differentiation of two radionuclides contamination by double radionuclide autoradiogra-phy? especially? monitoring of low level of radionuclides contamination by fluorescence sensitization autoradiography. The sensitivity of autoradiographic formation was increased by the scintillator by 10 times.
粪样
fecal sample
查询“粪样”译词为用户自定义的双语例句
相关查询
2008 CNKI－中国知网