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The object of this research is to make sure of the application of acid insoluble ash(AIA)as indicator to the dairy goat dagestion trials. Six Xi Nong Saanen dairy goats were selected and tests were made in specially constructed metabolism cages. The means and standard deviations of the recoveries of natural indicators SiO_and AIA(concentrated HCl,4N HCl and 2N HCl)in the goatf ecals are 97.9±10.5%,96.7±1.96,100.7±1.23% and 105.7±1.98% respectively.It is therefore concluded that only the mean recovery of 4N HCl-AIA... The object of this research is to make sure of the application of acid insoluble ash(AIA)as indicator to the dairy goat dagestion trials. Six Xi Nong Saanen dairy goats were selected and tests were made in specially constructed metabolism cages. The means and standard deviations of the recoveries of natural indicators SiO_and AIA(concentrated HCl,4N HCl and 2N HCl)in the goatf ecals are 97.9±10.5%,96.7±1.96,100.7±1.23% and 105.7±1.98% respectively.It is therefore concluded that only the mean recovery of 4N HCl-AIA is not significantly different(P>0.05)from 100%.The digestibility of dry matter, crude ash,gross energy,ether extract,crude fiber,crude protein and nitrogen- free-extract in ration determined by SiO_2 and AIA indicator mentioned above showed that only all the values determined by the method of 4N HCl-AIA near (P>0.05)the traditional method of collecting totol fecal.After quantitative feeding,the fecal samples were collected and the 2N HCl-AIA content dairy was analysed for two weeks.From ihe 6th day on,constant(P>0.05)value determined daily appeared.The 2N HCl-AIA contents of the fecal samples in different fecal collec-tion period were also determined,and the mixed samples of the 6th,7th,and 8th days showed no significant difference(P>0.05). On the 15th day after quantitativa feeding the fecal samples were collected at interval of 4-hour a day(6 times ewery day)and analysed.The 4N HCl-AIA contents of the fecal samples collected at any 4-hour interval showed no signiticant difference(P>0.05)from the whole fecal samplescollected in the same day. 试验旨在研究酸不溶灰分(Aeid Insoluble Ash,缩写为 AIA)在奶山羊消化试验中的应用。选择西农莎能奶山羊六只,在特制代谢笼内试验。试验测得内指示剂 SiO_2 及 AIA(浓 HCl、4NHCl 和2NHCl)在粪中的回收率分别为97.9±1.05%、96.7±1.96%、100.7±1.23%和105.7±1.98%。用 SiO_2 及三种 AIA 为指示剂测定日粮中干物质、粗灰分、总能、醚浸出物、粗纤维、粗蛋白质及无氮浸出物的消化率,除 4N HCl-AIA 法的各项测值接近于(P>0.05)传统的全粪收集法外,其它指示剂法均有一定差异。定量饲喂后,连续两个星期,逐日收集粪样,并分析其 2NHCl-AIA 含量,结果指出;从第六日始,各日测值趋于稳定(P>0.05)。试验测定了收粪期长短不同的粪样中2NHCl-AIA 含量,结果:6天、7天和8天的混合粪样差异不显著(P>0.05).于定量饲喂后的第15天,每隔4小时收集粪样一次,并分析之,结果:一天内任一4小时所采不完全粪样的4NHCl-AIA 含量同该日全粪收集样本未见有明显... 试验旨在研究酸不溶灰分(Aeid Insoluble Ash,缩写为 AIA)在奶山羊消化试验中的应用。选择西农莎能奶山羊六只,在特制代谢笼内试验。试验测得内指示剂 SiO_2 及 AIA(浓 HCl、4NHCl 和2NHCl)在粪中的回收率分别为97.9±1.05%、96.7±1.96%、100.7±1.23%和105.7±1.98%。用 SiO_2 及三种 AIA 为指示剂测定日粮中干物质、粗灰分、总能、醚浸出物、粗纤维、粗蛋白质及无氮浸出物的消化率,除 4N HCl-AIA 法的各项测值接近于(P>0.05)传统的全粪收集法外,其它指示剂法均有一定差异。定量饲喂后,连续两个星期,逐日收集粪样,并分析其 2NHCl-AIA 含量,结果指出;从第六日始,各日测值趋于稳定(P>0.05)。试验测定了收粪期长短不同的粪样中2NHCl-AIA 含量,结果:6天、7天和8天的混合粪样差异不显著(P>0.05).于定量饲喂后的第15天,每隔4小时收集粪样一次,并分析之,结果:一天内任一4小时所采不完全粪样的4NHCl-AIA 含量同该日全粪收集样本未见有明显差异(P>0.05)。 Four human rotavirus strains were isolated from 7 fecal specimens of pediatric patients with acute diarrhea and underwent 14 passages in CV-1 cell culture.The replication and specificity of these isolates in cell culture were confirmed by characteristic cytopathic effects, EM, ELISA, IF and viral RNA electrophoresis. All of the 4 isolates were long electrophero-type and no difference in RNA patterns was observed between the original fecal extract and the strain adapted in CV-1 cell culture for 7-14 passages. 用CV-1细胞从急性腹泻病儿7份粪便标本中直接分离出4株人轮状病毒(HRV),并适应传代14代。感染细胞出现特征性细胞病变,经电镜、ELISA、免疫荧光染色及病毒RNA电泳等试验证实HRV毒株在CV-1细胞中的繁殖及抗原特异性。病毒滴度为10~(6.0)TCID_(60)。分离的4株HRV毒株均为RNA电泳型长型,经CV-1细胞传7~14代后,RNA图型与原粪样相比未见变异。 In view of the exceptionally important role of the medical radiation protection in human health? in this paper we try to study on the monitoring of internal contamination of radionuclides in organism by different autoradiogra-phic methods* such as: monitoring of the body retention of isolated or combined radionuclides by freezing microautoradiography; monitoring of blood>bone marrow and excreta radioactive samples by smear autoradiographyi differentiation of two radionuclides contamination by double radionuclide... In view of the exceptionally important role of the medical radiation protection in human health? in this paper we try to study on the monitoring of internal contamination of radionuclides in organism by different autoradiogra-phic methods* such as: monitoring of the body retention of isolated or combined radionuclides by freezing microautoradiography; monitoring of blood>bone marrow and excreta radioactive samples by smear autoradiographyi differentiation of two radionuclides contamination by double radionuclide autoradiogra-phy? especially? monitoring of low level of radionuclides contamination by fluorescence sensitization autoradiography. The sensitivity of autoradiographic formation was increased by the scintillator by 10 times. 作者考虑到医学辐射防护对人类健康的特别重要性,为了能及时监测机体受放射性核素内污染的情况,研究设计了用各种不同水平的放射自显影术进行不同条件内污染的监测。先后摸索成功了用冰冻微观放射自显影术来监测体内呈游离态和结合态的内污染核素;涂片放射自显影对污染机体的血、髓、尿和粪样进行监测;并设计探讨用双标记放射自显影术对复合污染的核素进行鉴别监测;尤其是我们摸索成功的将闪烁液直接渗入液体乳胶中的荧光增敏放射自显影术,可以提高灵敏度达10倍之多,从而显著提高对低水平内污染核素的监测能力。
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